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- 저자 : Amit Pratap (검색결과 3 건)
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Bodi Surya Pratap Chandra Kishore,Amit Kumar,Purabi Saikia,Nikhil Lele,Arvind Chandra Pandey,Parul Srivastava,Bimal Kumar Bhattacharya,Mohamed Latif Khan 국립중앙과학관 2020 Journal of Asia-Pacific Biodiversity Vol.13 No.4
The present study focused on forest type classification and major plant species assemblages in Mudumalaiforest region using Airborne Visible/Infrared Imaging Spectrometer Next Generation. The phytosociologicalanalysis exhibited a total of 657 individuals (1095 individuals ha-1) of 24 tree speciesbelonging to 22 genera and 18 families. The highest tree density was contributed by Tectona grandis (132individuals ha-1 and 12.05% of total tree density) followed by Anogeissus latifolia (105 individuals ha-1 and9.59% of total tree density). The support vector machine study showed the dominance of SouthernTropical Semi-Evergreen forests (31%) followed by Southern Tropical moist deciduous forests (26.7%) andSouthern Tropical dry deciduous forests (24.8%) with a very high accuracy (92.37%). The comparativeanalysis of the existing forest types with Champion and Seth’s (1968) classification of forests exhibited achange of 30% in forest types in terms of their structure, composition, and extent over a period of 50years. The spectral angle mapperebased study emphasized the defining role of elevation, rainfall, andtemperature in species distribution, and physiognomy with dominance of A. latifolia (w19.22%). Thestudy implies the high spectral fidelity of airborne images for forest type mapping and plant speciesdiscrimination in tropical forests.
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Akash Bhangale,Sushant Wadekar,Sandeep Kale,Amit Pratap 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4
Biosurfactants are amphipathic molecules,consisting of hydrophobic and hydrophilic domains, which can partition at the interfaces between different fluid phases,such as oil/water or water/air interfaces. Mannosylerithritol lipids (MELs) are a type of glycolipid. The yeast Candida (Pseudozyma) antarctica (ATCC 32657) secretes an extracellular MEL, with biosurfactant properties, when grown on a water soluble and insoluble substrate, such as glycerine or oils. Value added MEL was biologically synthesized, by using two different types of honey as natural and newer water soluble carbon source. Those types of honey as a carbon source were optimized at different concentration of total carbohydrate present in the honey. Further optimized substrate concentration of honey was monitored for 15 days,for studies of MEL yield and cell growth concentration per day on shake flask batch culture. Surfactant properties,such as surface tension, interfacial tension, foaming,emulsification and wetting of MEL, were observed. Produced MEL was confirmed by thin layer chromatography (TLC),Fourier Transform Infrared (FTIR) spectra, and 1H Nuclear Magnetic Resonance (NMR) techniques.
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( Ritika Rampal ),( Saurabh Kedia ),( Mohamad Nahidul Wari ),( Deepak Madhu ),( Amit Kumar Singh ),( Veena Tiwari ),( V. Pratap Mouli ),( Srikant Mohta ),( Govind Makharia ),( Vineet Ahuja ) 대한장연구학회 2021 Intestinal Research Vol.19 No.2
Background/Aims: Crohn’s disease (CD) and intestinal tuberculosis (ITB) remain “difficult-to-differentiate” diseases. We have previously documented peripheral blood frequency of CD4<sup>+</sup>CD25<sup>+</sup>FOXP3<sup>+</sup> T-regulatory cells (Treg) as a biomarker to differ entiate CD and ITB. We tried to validate these results in a larger cohort of CD and ITB patients. Methods: Seventy treatment naïve patients of CD (n=23) and ITB (n=47) (diagnosed by standard criteria) were recruited prospectively from October 2016 to May 2017. Patients with history of antitubercular therapy in the past were excluded. The frequency of Treg cells in periph eral blood was determined by flow cytometry, and compared between CD and ITB patients. Results: Similar to our previous study, frequency of Treg cells in peripheral blood was significantly increased in ITB as compared to CD patients (40.9 [inter quartile range, 33-50] vs. 24.9 [interquartile range, 14.4-29.6], P< 0.001). Further, the receiver operating characteristics curve also showed good diagnostic accuracy with an area under the curve (AUC) of 0.77 (95% confidence interval, 0.65-0.89) and a FOXP3<sup>+</sup> cutoff value of >31.3% had a sensitivity and specificity of 83% and 82.6% respectively, to differentiate ITB from CD. Even for the indeterminate cases (n=33), Treg cell frequency had similar diagnostic accuracy with an AUC of 0.85 (95% confi dence interval, 0.68-0.95) and a cutoff of 32.37% had sensitivity and specificity of 87% and 95% respectively, to differentiate ITB from CD. Conclusions: The current findings validate that the increased frequency of CD4<sup>+</sup>CD25<sup>+</sup>FOXP3<sup>+</sup> Treg in the peripheral blood can be used as a biomarker with high diagnostic accuracy to differentiate ITB from CD. (Intest Res 2021;19:232-238)
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