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RISS 인기검색어
- 검색키워드
- 저자 : Alsberg, Eben (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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Highly Porous Electrospun Nanofibers Enhanced by Ultrasonication for Improved Cellular Infiltration
Lee, Jung Bok,Jeong, Sung In,Bae, Min Soo,Yang, Dae Hyeok,Heo, Dong Nyoung,Kim, Chun Ho,Alsberg, Eben,Kwon, Il Keun Mary Ann Liebert 2011 Tissue engineering. Part A Vol.17 No.21
<P>A significant problem that affects tissue-engineered electrospun nanofibrous scaffolds is poor infiltration of cells into the three-dimensional (3D) structure. Physical manipulation can enhance cellular infiltration into electrospun scaffolds. The porosity of electrospun nanofibers was highly enlarged by ultrasonication in an aqueous solution. The porosity and related property changes on a series of nanofibers were observed to be dependent on ultrasonication time and energy. To evaluate cell infiltration into the scaffold, fibroblasts were seeded onto these nanofibers and cultured for different lengths of time. The penetration levels of these cells into the scaffold were monitored using confocal lazer scanning microscopy. The cell infiltration potential was greatly increased with regard to an increase in pore size and porosity. These 3D nanofibrous scaffolds fabricated by an ultrasonication process allowed cells to infiltrate easily into the scaffold. This approach shows great promise for design of cell permeable nanofibrous scaffolds for tissue-engineering applications.</P>
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Jeong, Sung In,Burns, Nancy A.,Bonino, Christopher A.,Kwon, Il Keun,Khan, Saad A.,Alsberg, Eben The Royal Society of Chemistry 2014 Journal of Materials Chemistry B Vol.2 No.46
<P>A significant problem affecting electrospun nanofibrous tissue scaffolds is poor infiltration of cells into their three-dimensional (3D) structure. Environmental and physical manipulation, however, can enhance cellular infiltration into electrospun scaffolds. In this work, RGD-modified alginate mats with increased thickness and porosity were achieved by pairing high humidity electrospinning with post-processing ultra-sonication. RGD-modified alginate, polyethylene oxide (PEO), and an FDA-approved, nonionic surfactant blends were electrospun in 20 and 50% relative humidity conditions. Mats electrospun in high humidity conditions resulted in significantly increased mat thickness and decreased fiber diameters. The mats' alginate content was then isolated <I>via</I> ionic crosslinking and PEO/surfactant extraction. Finally, the alginate-only mat was post-processed by ultra-sonication to further enhance its cross-sectional thickness. Cell morphology, proliferation, and infiltration into the scaffolds were evaluated by seeding fibroblasts onto the alginate mat. Cell spreading, growth and infiltration improved with increased humidity and ultra-sonication. This approach shows great promise for the design of cell-permeable nanofibrous scaffolds for tissue-engineering applications.</P>
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Seo, Jungmok,Shin, Jung-Youn,Leijten, Jeroen,Jeon, Oju,Bal Ö,ztü,rk, Ayç,a,Rouwkema, Jeroen,Li, Yuancheng,Shin, Su Ryon,Hajiali, Hadi,Alsberg, Eben,Khademhosseini, Ali American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.16
<P>Biophysical cues can potently direct a cell’s or tissue’s behavior. Cells interpret their biophysical surroundings, such as matrix stiffness or dynamic mechanical stimulation, through mechanotransduction. However, our understanding of the various aspects of mechanotransduction has been limited by the lack of proper analysis platforms capable of screening three-dimensional (3D) cellular behaviors in response to biophysical cues. Here, we developed a dynamic compression bioreactor to study the combinational effects of biomaterial composition and dynamic mechanical compression on cellular behavior in 3D hydrogels. The bioreactor contained multiple actuating posts that could apply cyclic compressive strains ranging from 0 to 42% to arrays of cell-encapsulated hydrogels. The bioreactor could be interconnected with other compressive bioreactors, which enabled the combinatorial screenings of 3D cellular behaviors simultaneously. As an application of the screening platform, cell spreading, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) were characterized in 3D gelatin methacryloyl (GelMA) hydrogels. Increasing hydrogel concentration from 5 to 10% restricted the cell spreading, however, dynamic compressive strain increased cell spreading. Osteogenic differentiation of hMSCs was also affected by dynamic compressive strains. hMSCs in 5% GelMA hydrogel were more sensitive to strains, and the 42% strain group showed a significant increase in osteogenic differentiation compared to other groups. The interconnectable dynamic compression bioreactor provides an efficient way to study the interactions of cells and their physical microenvironments in three dimensions.</P> [FIG OMISSION]</BR>
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