Solubilization of Proteins from Human Lymph Node Tissue and Two-Dimensional Gel Storage

Alessandra Bernadete Trovo De Marqui,Alessandra Vidotto,Giovana Mussi Polachini,Claudia De Mattos Bellato,Hamilton Cabral,Andreia Machado Leopoldino,Jose Francisco De Gois Filho,Erica Erina Fukuyama,F 한국생활환경학회 2006 BMB Reports Vol.39 No.2

Solubilization of Proteins from Human Lymph Node Tissue and Two-Dimensional Gel Storage

De Marqui, Alessandra Bernadete Trovo,Vidotto, Alessandra,Polachini, Giovana Mussi,De Mattos Bellato, Claudia,Cabral, Hamilton,Leopoldino, Andreia Machado,De Gois Filho, Jose Francisco,Fukuyama, Erica Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.2

In the present study, we compared six different solubilization buffers and optimized two-dimensional electrophoresis (2-DE) conditions for human lymph node proteins. In addition, we developed a simple protocol for 2-D gel storage. Efficient solubilization was obtained with lysis buffers containing (a) 8M urea, 4% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate), 40 mM Tris base, 65 mM DTT(dithiothreitol) and 0.2% carrier ampholytes; (b) 5M urea, 2M thiourea, 2% CHAPS, 2% SB 3-10 (N-decyl-N, N-dimethyl-3-ammonio-1-propanesulfonate), 40mM Tris base, 65 mM DTT and 0.2% carrier ampholytes or (c) 7M urea, 2M thiourea, 4% CHAPS, 65 mM DTT and 0.2% carrier ampholytes. The optimal protocol for isoelectric focusing (IEF) was accumulated voltage of 16,500 Vh and 0.6% DTT in the rehydration solution. In the experiments conducted for the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), best results were obtained with a doubled concentration (50 mM Tris, 384 mM glycine, 0.2% SDS) of the SDS electrophoresis buffer in the cathodic reservoir as compared to the concentration in the anodic reservoir (25 mM Tris, 192 mM glycine, 0.1% SDS). Among the five protocols tested for gel storing, success was attained when the gels were stored in plastic bags with 50% glycerol. This is the first report describing the successful solubilization and 2D-electrophoresis of proteins from human lymph node tissue and a 2-D gel storage protocol for easy gel handling before mass spectrometry (MS) analysis.

Recurrent Optic Neuritis as the Initial Symptom in Demyelinating Diseases

Alessandra Billi,Falcão-Gonçalves,Denis Bernardi Bichuetti,Enedina Maria Lobato de Oliveira 대한신경과학회 2018 Journal of Clinical Neurology Vol.14 No.3

Background and Purpose Optic neuritis (ON) is an inflammation of the optic nerve that can be recurrent, with unilateral or bilateral presentation. Diagnosing recurrent cases may be challenging. We aimed to compare patients with recurrent ON as their initial symptom according to their following final diagnoses: multiple sclerosis (MS), neuromyelitis optica spectrum disorders (NMOSD), or chronic relapsing inflammatory optic neuropathy (CRION). Methods The medical records of patients with initial recurrent ON who were followed at the Neuroimmunology Clinic of the Federal University of São Paulo between 2004 and 2016 were analyzed retrospectively. Patients were classified according to their final diagnosis into MS, NMOSD, or CRION, and the characteristics of these groups were compared to identify predictive factors. Results Thirty-three patients with recurrent ON were included, and 6, 14, and 13 had final diagnoses of MS, NMOSD, and CRION, respectively. Most of the patients were female with unilateral and severe ON in their first episode, and the initial Visual Functional System Score (VFSS) was ≥5 in 63.6%, 85.7%, and 16.7% of the patients with CRION, NMOSD, and MS, respectively. Anti-aquaporin-4 antibodies were detected in 9 of 21 (42.8%) tested patients. Seven of nine (77.8%) seropositive NMOSD patients experienced transverse myelitis episodes during the follow-up period. A multivariate regression analysis showed that the VFSS at the last medical appointment predicted the final diagnosis. Conclusions A lower VFSS at the last medical appointment was predictive of MS. Patients with NMOSD and CRION have similar clinical characteristics, whereas NMOSD patients tend to have worse visual acuity.

Role of 18F-FDG PET/CT in the Carcinoma of the Uterus: A Review of Literature

Alessandra Musto,Domenico Rubello,Gaia Grassetto,Maria Cristina Marzola,Sotirios Chondrogiannis,Anna Margherita Maffione,Lucia Rampin,David Fuster,Francesco Giammarile,Patrick M. Colletti 연세대학교의과대학 2014 Yonsei medical journal Vol.55 No.6

In the present review we reported the value of 18F-fluorodeoxyglucose (FDG) PET/CT in face of uterine cancer, in terms of sensitivity, specificity and accuracy. Moreover,we made a comparison with the other imaging techniques currently used to evacuate these tumors including contrast-enhanced CT, contrast enhanced-MRI and transvaginal ultrasonography. FDG PET/CT has been reported to be of particularvalue in detecting occult metastatic lesions, in prediction of response to treatmentand as a pro-gnostic factor.

Somatosensory Conduction Pathway in Spastic Paraplegia Type 5

Alessandra Vanotti,Lorenzo Nanetti,Davide Rossi Sebastiano,Elisa Visani,Dunja Duran,Daniela Di Bella,Elisa Sarto,Claudio Caccia,Valerio Leoni,Franco Taroni,Caterina Mariotti 대한신경과학회 2014 Journal of Clinical Neurology Vol.10 No.4

Robotic versus Laparoscopic versus Open Gastrectomy: A Meta-Analysis

Alessandra Marano,형우진,최윤영,김유민,김지은,노성훈 대한위암학회 2013 Journal of gastric cancer Vol.13 No.3

Purpose: To define the role of robotic gastrectomy for the treatment of gastric cancer, the present systematic review with meta-analysis was performed. Materials and Methods: A comprehensive search up to July 2012 was conducted on PubMed, EMBASE, and the Cochrane Library. All eligible studies comparing robotic gastrectomy versus laparoscopic gastrectomy or open gastrectomy were included. Results: Included in our meta-analysis were seven studies of 1,967 patients that compared robotic (n=404) with open (n=718) or laparoscopic (n=845) gastrectomy. In the complete analysis, a shorter hospital stay was noted with robotic gastrectomy than with open gastrectomy (weighted mean difference: -2.92, 95% confidence interval: -4.94 to -0.89, P=0.005). Additionally, there was a significant reduction in intraoperative blood loss with robotic gastrectomy compared with laparoscopic gastrectomy (weighted mean difference:-35.53, 95% confidence interval: -66.98 to -4.09, P=0.03). These advantages were at the price of a significantly prolonged operative time for both robotic gastrectomy versus laparoscopic gastrectomy (weighted mean difference: 63.70, 95% confidence interval: 44.22to 83.17, P<0.00001) and robotic gastrectomy versus open gastrectomy (weighted mean difference: 95.83, 95% confidence interval:54.48 to 137.18, P<0.00001). Analysis of the number of lymph nodes retrieved and overall complication rates revealed that these outcomes did not differ significantly between the groups. Conclusions: Robotic gastrectomy for gastric cancer reduces intraoperative blood loss and the postoperative hospital length of stay compared with laparoscopic gastrectomy and open gastrectomy at a cost of a longer operating time. Robotic gastrectomy also provides an oncologically adequate lymphadenectomy. Additional high-quality prospective studies are recommended to better evaluate both short and long-term outcomes.

Deux Manifestes Poétiques chez Ennius : Annales, fr. 1 et 206‐211 Skutsch

Alessandra Romeo 韓國西洋古典學會 2012 西洋古典學硏究 Vol.49 No.-

The Prooemia, preserved in fragmentary form, which are assigned to books 1 and 7 of the Annals of Ennius, combine the idea of the epic as a sacred song (the story of Rome being the subject of a laudative idealization) with the idea, inherited from Alexandrian poets “philologists”, of poetry as a learned technique. With verses that probably open the poem, Musae quae pedibus magnum pulsatis Olympum (fr. Skutsch 1), Ennius takes openly stand in the Homeric tradition: he usually refers to Homer’s epic model and the poetic function it represented, to inherit him the rôle of initiator (the verse was probably followed by the episode of the dream in which Ennius was consecrated ‘Homer reincarnated’). But at the same time Ennius proclaims himself the first poet dicti studiosus: he suggests an image of himself not only as vates but also as primus inventor of a learned poetry that creates a new language and a new rhythm. This selfdefinition finds in the Prooemium a favourable space: it is, however, a particular Prooemium since it is not at the beginning of the work but at the beginning of Book 7. Ennius presents his poem and its originality in relation to the topos of primus ego in two Prooemia: at the beginning of the poem, in the form of a dream initiatory as a moment of poetic investiture according to the Theogony, and then at the beginning of Book 7 in literary terms, making an agonistic reference to Naevius. The political function and duty of the Roman poet requires a poetic form that defines new literary terms. This definition is the only one that can reflect the rôle of the poet and express the change of the poetic function in relation to the model of the poet “philologist” of Alexandrian tradition.

Effect of Drag Stages Surface Roughness on the Compression Ratio of a TMDP

Alessandra Dal Bianco,Luca Bonmassar 한국진공학회 2016 Applied Science and Convergence Technology Vol.25 No.6

The rotor of a turbomolecular drag pump is generally made of an aluminum alloy. Its surface finish is affected by various processes that the rotor itself undergoes during the manufacturing phase. The impact of different surface finishes on the pumping performances of a turbomolecular pump has been mainly investigated by Sawada et al [1]. The present work aims to broaden the previous bibliographic study to the drag stages of a turbomolecular pump by testing the impact of different surface finishes on the compression ratio of the pump. Experimental tests have been made focusing on two processes: the corundum sandblasting and the glass microspheres shot-peening. Both the processes flatten and/or physically remove EDM melted spheres; in particular, blasted surfaces obtained by glass shotpeening are generally smoother than surfaces obtained by corundum sandblasting. In order to characterize the surface texture left by such processes, preliminary surface roughness measurements have been made on the drag rotor disks of several pumps. The experimental tests conducted on both sandblasted and shot-peened rotors confirms previous results obtained on the turbo stages by Sawada et al. [1], showing that the average roughness of the surface has an impact on the compression ratio of the pump; in particular, an increment in the surface roughness causes a corresponding increment in the compression ratio of the pump and vice versa. For the tested pumps, the higher surface roughness gives a factor of increment of about 2 on the measured hydrogen maximum compression ratio of the pump.

Low-Dose Mepolizumab Effectiveness in Patients Suffering From Eosinophilic Granulomatosis With Polyangiitis

Alessandra Vultaggio,Francesca Nencini,Susanna Bormioli,Emanuele Vivarelli,Laura Dies,Oliviero Rossi,Paola Parronchi,Enrico Maggi,Andrea Matucci 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.5

Eosinophilic granulomatosis with polyangiitis (EGPA) is a vasculitis characterized by multisystemic manifestations including asthma. Mepolizumab (300 mg/4 weeks) has recently been approved for EGPA. However, real-life data are scarce and report experiences with high doses of mepolizumab intravenously administered (750 mg/4 weeks). The aim of our study was to investigate in a real-life setting whether mepolizumab in EGPA patients at low doses would enable us 1) to control asthma symptoms, 2) to obtain oral corticosteroids (OCS) and/or immunosuppressors tapering and 3) to maintain clinical remission and avoid disease relapses. Mepolizumab (100 mg/4 weeks) was subcutaneously administered for 12 months in 18 EGPA patients with uncontrolled severe asthma. Symptoms, annual asthma exacerbation rates, OCS-sparing effects, lung function and eosinophil activation markers were monitored. The proportion of patients with clinical remission or relapse was also evaluated in month 12. A significant decrease in the annual rate of asthma exacerbations in association with significant changes in asthma control were observed. Specifically, 66.6% of the patients experienced no exacerbations during the mepolizumab treatment. Most patients (77.7%) were able to reduce the daily OCS dose by at least 50%. Four patients also stopped cyclosporine A during the study period. No EGPA relapse was observed and a large majority of the patients achieved clinical remission (94.3%). Clinical benefits were paralleled by reduction in blood eosinophils and serum levels of eosinophil activation markers. Low-dose mepolizumab showed clinically relevant benefits in exacerbation rates, asthma symptoms, OCS and immunosuppressive use in EGPA patients. These effects occurred without any EGPA relapse for extrapulmonary manifestations.

Elastoplastic response and recoil of lattice structures under hyperbolic hardening

Alessandra Bonfanti,Stavros Syngellakis,Atul Bhaskar 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.4

Elastoplastic response and recoil analyses for hexagonal honeycomb lattice structures are presented when hardening is described by a hyperbolic law. By exploiting the translational symmetry of the problem, the analysis is reduced to that of a thin beam under combined bending and axial loading coupled with the kinematics of lattice deformation and its relationship with cell wall deformation. A closedform solution for the load-curvature relationship of a beam with rectangular cross-section is obtained. A systematic study of beam response, as the stress-strain curve of the constituent material approaches an ideal elastic-perfectly plastic law, is presented. The analysis is then applied to an infinite honeycomb sheet under remote tensile load to obtain the apparent non-linear structural response. Apparent recoil of such a lattice material upon unloading is also calculated in closed form, when unloading is assumed to take place along a linear stress-strain curve. The analytical results are in excellent agreement with the numerical calculations.