An Approach to Plant Identification Technology

Tomoo Aoyama,Ryo Akashi,Hidenori Umeno,Umpei Nagshima 제어로봇시스템학회 2006 제어로봇시스템학회 국제학술대회 논문집 Vol.2006 No.10

Nutritive Evaluation of Forage Plants Grown in South Sulawesi, Indonesia II. Mineral Composition

Nasrullah, Nasrullah,Niimi, Mitsuhiro,Akashi, Ryo,Kawamura, Osamu Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.1

In order to evaluate the nutritive value of the forage plants in South Sulawesi, Indonesia, 266 samples (61 grasses and 65 legumes grown in the dry season, 60 grasses and 80 legumes grown in the rainy season) were collected from the highland and the lowland in 1998 to 2000, and were subjected to the determination of mineral composition. The mean contents of Ca, Mg, P, Na and K in grasses were 0.6, 0.3, 0.5, 0.1 and 2.3%, respectively, and in legumes were 1.8, 0.5, 0.8, 0.1 and 1.7%, respectively. The least-squares analysis of variance demonstrated as follows; For the grasses, Ca content was significantly affected by the year${\times}$season, year${\times}$altitude, and the season${\times}$altitude interactions. Mg content was significantly affected by year and season and P content was significantly affected by altitude, year and by the year${\times}$altitude interaction. For the legumes, Ca content was significantly affected by altitude and the year${\times}$season, season${\times}$altitude and the year${\times}$altitude interactions and Mg content was significantly affected by season or altitude and by the year${\times}$season interaction. These results indicate that Ca content of forage plants grown at the lowland in rainy season was higher than at the highland in dry season. Mg content of forage plants grown at the lowland in dry season was higher than at the highland in rainy season and P content of forage plants grown in the highland was higher than in the lowland.

Morphological Traits of Lotus japonicus (Regal) Ecotypes Collected in Japan

Hashiguchi, Masatsugu,Tsuruta, Shin-Ichi,Akashi, Ryo Korean Society for Bioinformatics 2011 Interdisciplinary Bio Central (IBC) Vol.3 No.1

Forty-seven wild accessions of Lotus japonicus Regal (Japanese trefoil) indigenous to Japan were investigated for nine morphological characters. Average temperature and annual precipitation were negatively correlated with stem color and seed weight. On the other hand, latitude was positively correlated with these traits. Consequently, accessions from sites at higher latitudes with low temperatures and precipitation tend to have dark red stems and heavy seeds. Cluster analysis based on nine morphological characters classified 47 wild accessions into six major groups. Cluster I included four accessions of tall and erect plants. These plants are phenotypically similar to commercial variety 'Empire'. Cluster II consisted of three accessions of creep plants with pale red stems. Cluster III contained 24 accessions that had average values for all morphological characters evaluated. Cluster IV included two accessions of erect plants with rounded leaflets and dark red stems. Cluster V included four accessions of small, creep plants with pale red stems. Cluster VI included seven accessions of small and erect plants, a phenotype that also applies to ?Gifu B-129?, which is used as experimental strain worldwide. These data were deposited into LegumeBase, an online database (http://www.legumebase.brc.miyazaki-u.ac.jp/) supported by the National BioResource Project (NBRP) in Japan.

Biochemical changes in recombinant inbred lines of soybean (Glycine max L. Merr.) under storage-related stresses

Jiamtae Pakanan,Hashiguchi Takuyu,Pongpiyapaiboon Sorawich,Lelapiyamit Intouch,Tanaka Hidenori,Hashiguchi Masatsugu,Akashi Ryo 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.4

Soybean is a major crop that is rich in proteins, lipids, and isofavones. Although the seeds are widely used for food and industrial purposes, soybean is recognized as a crop that does not store well, as storage conditions greatly afect seed quality. In this study, we selected 10 recombinant inbred lines (RILs) of soybean that are either sensitive or tolerant to storage, and examined the biochemical characteristics of the lines and their parents under storage-related stresses. The germination rates of all of the lines in the tolerant group were higher than those of the sensitive group for all storage conditions. Malondialdehyde (MDA) content was higher in the sensitive group than in the tolerant group, with the MDA content typically increasing under long-term storage conditions. Superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities were also more afected by long-term storage, but not by higher temperatures. Isofavones were also assayed and it was found that the aglycones (except glycitein) increased with an increase of storage temperature. Malonyl isofavone glucosides tended to decrease under long-term storage and high temperature conditions, suggesting that malonyl glucosides are converted to their aglycones. These results illustrate the mechanisms underlying storage-related stresses and the importance of screening for lines that can tolerate environmental stresses.

Short-term grazing behavior of cattle under indoor housing for a new-bred tetraploid ruzigrass (Brachiaria ruziziensis Germain et Everard)

Ishigaki, Genki,Nitthaisong, Pattama,Prasojo, Yogi Sidik,Kobayashi, Ikuo,Fukuyama, Kiichi,Rahman, Mohammad Mijanur,Akashi, Ryo Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.5

Objective: The preference evaluation of cattle is an important factor for estimation and improvement of the grazing amounts of newly introduced or bred grasses or cultivars in barn. This study was performed to assess the grazing behavior (the amount of grazing and/or the grazing speed) of cattle as indirect method using newly bred Brachiaria ruziziensis tetraploid strain 'OKI-1'(BR) hay as treatment group and Cloris gayana 'Callide' (CG) hay as control group. It also compared the feasibility of using behavioral differences between two groups as one criteria for evaluating preference by Japanese black cattle in barn. Methods: Three experiments were carried out using 12 growing Japanese Black cattle including 6 males and 6 females. In each experiment, the four Japanese Black cattle (2 males and 2 females) were placed in separated stall and allowed to graze BR and CG in manger that was separated into two portions for about 30 min. The position and behavior of the cattle were recorded, and weighed the residual of each gay at 15 and 30 minutes after experiment start. Results: The BR was superior to CG in chemical composition such as protein, fibers and non-fibrous carbohydrate. The cattle, over all, tended to prefer BR over CG in the first half 15 minutes in terms of the time spent and amount of grazing. Additionally, growing cattle exhibited neophilia for BR bred newly. Conclusion: These findings indicated the current approach could be applied for one of criteria to evaluate the preference of hay by Japanese black cattle under indoor housing environment.

Particle Inflow Gun을 이용한 벼 캘러스 내의 효율적 유전자 도입

송인자,배창휴,최대옥,이효연,Song, In-Ja,Bae, Chang-Hyu,Choi, Dae-Ock,Ryo Akashi,Lee, Hyo-Yeon 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.4

본 연구실에서 개발된 particle inflow gun (PIG)은 조작이 간편하고, 사용비용도 저렴하며, 식물 세포 내의 유전자 도입효율이 높은 특징을 갖고 있다. PIG 장비를 이용하여 벼 캘러스 내로의 유전자 도입 조건을 검토하기 위해서 사용된 vector는 pIG121Hm으로서 T-DNA 내부에 intron GUS ($\beta$-glucuronidase)와 hygromycin 및 kanamycin 저항성 유전자를 포함하고 있다. 또한 벼 캘러스 내에 물리적으로 DNA를 도입할 때에 DNA 도입 효율과 관계가 높은 요인들을 GUS의 발현빈도를 통하여 조사하였다. 그 결과 gold particle에 DNA를 부착하는 과정에 사용되는 spermidine과 calcium chloride의 경우 무첨가구에 비해 16 mM의 spermidine과 1.5 M의 calcium chloride 첨가구에서 GUS 발현율이 각각 2배, 3배 증가하였다. 그리고 1회 분사되는 gold particles양이 2 mg의 경우 가장 높은 GUS 발현율을 보여주었으며, 또한 PIG장비의 분사거리와 헬륨의 압력은 벼의 배양세포의 경우 12cm의 분사거리에서 3.5 bar (50 psi)의 헬륨압력으로 분사하였을 때 GUS 발현율이 가장 높았다. 이상의 결과에서 PIG 장비를 이용한 유전자 도입은 본 연구에서 검토한 최적의 조건을 이용하였을 경우 기존에 많이 사용되고 있는 Biolistic Gun (Bio-Rad 사)과 거의 비슷한 유전자 도입효율을 보여 주었다. 특히 PIG 장비의 경우 조작이 매우 간편하고, 분사에 사용되는 일회용 부품이 필요하지 않기 때문에 대량의 반복실험을 필요로 하는 연구에서 손쉽게 사용되리라 기대된다. We have developed a homemade particle inflow gun (PIG) system which has simple operation method, low price and high gene introduction efficiency into rice callus. Rice callus were inflowed with gold particles containing DNA of a plasmid, pIG121Hm, harboring intron GUS ($\beta$-glucuronidase) gene, NPTII gene and HPT gene. For optimal GUS transient expression, the effects of parameters on DNA delivery efficiency of the PIG system was investigated by scoring transient GUS expression. The highest number of blue spots was observed at 16 mM of spemidine and 1.5 M of calcium chloride, respectively. And the amount of gold particles required for the best GUD expression was 2 mg. Optimum GUS transient expression was observed at target distance of 12 cm and helium pressure of 3.5 bar (50 psi). Gene introduction efficiency of the PIG system was observed almost similar to that of the Biolistic Gun (Bio-Rad Company). Since PIG system is simple to operate and one doesn't need disposable accessaries, the PIG system can be easily applied to various replication experiments.