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Identification of Glycine Max MicroRNAs in Response to Phosphorus Deficiency
Aihua Sha,Yinhua Chen,Hongping Ba,Zhihui Shan,Xiaojuan Zhang,Xuejun Wu,Dezheng Qiu,Shuilian Chen,Xinan Zhou 한국식물학회 2012 Journal of Plant Biology Vol.55 No.4
MicroRNAs (miRNAs) are endogenous small RNAs regulating plant development and stress responses. In addition, phosphorus (P) is an important macronutrient for plant growth and development. More than two hundred miRNAs have been identified in Glycine Max and a few of miRNAs have been shown to respond to P deficiency,however, whether there are other miRNAs involved in P deficiency response is largely unknown. In this study, we used high-throughput small RNA sequencing and wholegenome-wide mining to identify the potential miRNAs in response to P deficiency. After sequencing, we deduced 183known, 99 conserved and 126 novel miRNAs in Glycine Max. Among them, in response to P deficiency, the expressions of 27 known, 16 conserved and 12 novel miRNAs showed significant changes in roots, whereas the expressions of 34known, 14 conserved and 7 novel miRNAs were significantly different in shoots. Furthermore, we validated the predicated novel miRNAs and found that three miRNAs in roots and five miRNAs in shoots responded to P deficiency. Some miRNAs were P-induced whereas some were P-suppressed. Together these results indicated that the miRNAs identified might play important roles in regulating P signaling pathway.
The Association of SERPINE2 Gene with COPD in a Chinese Han Population
Aihua Wang,Wei Xiao,Yingqiu Yin,Ping Chen,Qiji Liu,Qinfeng Yu 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.6
Purpose: Polymorphisms of several candidate genes have been studied and associated with the development of chronic obstructive pulmonary disease (COPD). One such candidate is the SERPINE2 (Serpin peptidase inhibitor, clade E member 2) gene. Materials and Methods: To assess whether the SERPINE2 gene is associated with COPD in a Chinese Han population. Samples were collected from a Chinese Han population and analyzed for the association of single nucleotide polymor phisms (SNPs) or haplotypes of SERPINE2 gene with COPD in a case-control study. Three SNPs including rs840088 G/A in intron 1, rs1438831 A/G in 5’ upstream sequence and rs3795879 G/A in intron 3 were detected using the polymerase chain reaction (PCR)-based restriction fragment length polymorphism technique in 409 COPD subjects and 411 controls. Genotyping of the SREPINE2 polymorphisms at positions rs840088, rs1438831and rs3795879 was performed. Results: We found that none of the rs840088G/A, rs1438831G/A and rs3795879 G/A polymorphisms were associated with the disease. The p-values were 0.630, 0.208 and 0.398 respectively. Conclusion: Our data suggested that there was no significant association between SERPINE2 polymorphism and COPD susceptibility in the Chinese Han population.
Chen, Qian,Zhang, Zhijian,Liu, Jinbo,He, Qinghua,Zhou, Yuepeng,Shao, Genbao,Sun, Xianglan,Cao, Xudong,Gong, Aihua,Jiang, Ping Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.3
Because Schwann cells perform the triple tasks of myelination, axon guidance and neurotrophin synthesis, they are candidates for cell transplantation that might cure some types of nervous-system degenerative diseases or injuries. However, Schwann cells are difficult to obtain. As another option, ectomesenchymal stem cells (EMSCs) can be easily harvested from the nasal respiratory mucosa. Whether fibrin, an important transplantation vehicle, can improve the differentiation of EMSCs into Schwann-like cells (SLCs) deserves further research. EMSCs were isolated from rat nasal respiratory mucosa and were purified using anti-CD133 magnetic cell sorting. The purified cells strongly expressed HNK-1, nestin, $p75^{NTR}$, S-100, and vimentin. Using nuclear staining, the MTT assay and Western blotting analysis of the expression of cell-cycle markers, the proliferation rate of EMSCs on a fibrin matrix was found to be significantly higher than that of cells grown on a plastic surface but insignificantly lower than that of cells grown on fibronectin. Additionally, the EMSCs grown on the fibrin matrix expressed myelination-related molecules, including myelin basic protein (MBP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and galactocerebrosides (GalCer), more strongly than did those grown on fibronectin or a plastic surface. Furthermore, the EMSCs grown on the fibrin matrix synthesized more neurotrophins compared with those grown on fibronectin or a plastic surface. The expression level of integrin in EMSCs grown on fibrin was similar to that of cells grown on fibronectin but was higher than that of cells grown on a plastic surface. These results demonstrated that fibrin not only promoted EMSC proliferation but also the differentiation of EMSCs into the SLCs. Our findings suggested that fibrin has great promise as a cell transplantation vehicle for the treatment of some types of nervous system diseases or injuries.
Adaptive Sampling for Low Power Mobile Sign Language Video Communication
Xiaolei Chen,Aihua Zhang,Xinzhu Yang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.12
In this paper, we propose an adaptive sampling technique that achieves considerable energy savings while maintaining the required intelligibility level for mobile sign language video communication. The adaptive sampling scheme adjusts the sampling rate of the camera sensors dynamically according to the properties of sign language video communication and available battery power of mobile devices. Experimental results show that this adaptive scheme performs better than continuous sensing scheme in balancing the energy- intelligibility tradeoffs.
Ling Fan,Zhao Chen,Wenbin Fu,Nenggui Xu,Jianhua Liu,Aiping Lu,Ziping Li,Shengyong Su,Taixiang Wu,Aihua Ou 사단법인약침학회 2016 Journal of Acupuncture & Meridian Studies Vol.9 No.5
This study aims to investigate the molecular mechanisms of acupuncture in the remission of depression. A depressive disorder model was induced by exposing Sprague–Dawley rats to chronic unpredictable stress. The rats were divided into five groups: healthy (blank group) and stressed rats (model group), and stressed rats treated with acupuncture (acupuncture group), riluzole (riluzole group), acupuncture combined with botulinum toxin A (BTX-A) injection (acupuncture+BTX-A group) or riluzole combined with BTX-A injection (riluzole+BTX-A group). Behavioral analysis showed significant differences in sucrose consumption, weight, and horizontal or vertical movements between the model and both the riluzole and acupuncture groups. No obvious differences between the riluzole+BTX-A and acupuncture+BTX-A groups were found. Moreover, no significance differences in glutamate content in the hippocampus were found among the riluzole+BTX-A, acupuncture+BTX-A and model groups (p>0.05). Western blots and reverse transcription polymerase chain reactions were employed to detect protein and mRNA expressions of VGLUT2, SNAP25, VAMP1, VAMP2, VAMP7, and syntaxin1; no obvious differences among the riluzole+BTX-A, acupuncture+BTX-A and model groups were found. These data suggest that soluble N-ethylmaleimide-sensitive factor attachment receptor proteins are involved in the remission of depression in rats treated with acupuncture.
Zhijian Zhang,Qian Chen,Jinbo Liu,Qinghua He,Yuepeng Zhou,Genbao Shao,Xianglan Sun,Xudong Cao,Aihua Gong,Ping Jiang 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.3
Because Schwann cells perform the triple tasks of myelination, axon guidance and neurotrophin synthesis, they are candidates for cell transplantation that might cure some types of nervous-system degenerative diseases or injuries. However, Schwann cells are difficult to obtain. As another option, ectomesenchymal stem cells (EMSCs) can be easily harvested from the nasal respiratory mucosa. Whether fibrin, an important transplantation vehicle, can improve the differentiation of EMSCs into Schwann-like cells (SLCs) deserves further research. EMSCs were isolated from rat nasal respiratory mucosa and were purified using anti-CD133 magnetic cell sorting. The purified cells strongly expressed HNK-1, nestin, p75NTR, S-100, and vimentin. Using nuclear staining, the MTT assay and Western blotting analysis of the expression of cell-cycle markers, the proliferation rate of EMSCs on a fibrin matrix was found to be significantly higher than that of cells grown on a plastic surface but insignificantly lower than that of cells grown on fibronectin. Additionally, the EMSCs grown on the fibrin matrix expressed myelination-related molecules, including myelin basic protein (MBP), 2’,3’-cyclic nucleotide 3’-phosphodiesterase (CNPase) and galactocerebrosides (GalCer), more strongly than did those grown on fibronectin or a plastic surface. Furthermore, the EMSCs grown on the fibrin matrix synthesized more neurotrophins compared with those grown on fibronectin or a plastic surface. The expression level of integrin in EMSCs grown on fibrin was similar to that of cells grown on fibronectin but was higher than that of cells grown on a plastic surface. These results demonstrated that fibrin notonly promoted EMSC proliferation but also the differentiation of EMSCs into the SLCs. Our findings suggested that fibrin has great promise as a cell transplantation vehicle for the treatment of some types of nervous system diseases or injuries.
Liu, Xiping,Wang, Chen,Zheng, Aihua Journal of International Conference on Electrical 2012 Journal of international Conference on Electrical Vol.1 No.3
The operation principle of an axial flux-switching hybrid excitation synchronous machine (AFHESM) is analyzed and its topology structures are proposed in this paper. After some comprehensive analysis of the operation principle to axial flux electrical machine, flux-switching electrical machine and hybrid excitation electrical machine, the operation principle of AFHESM is given. Combined with some typical topological structures of hybrid excitation electrical machine, some possible topological structures are proposed and some comprehensive comparisons are carried out. The analysis results show that the stator-separated AFHESM has some advantages such as less AM turns, less impact on the demagnetization of PM, less magnetic flux-leakage and higher efficiency compared to other topologies.