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( Xing Ai Gao ),( Wan Taek Ju ),( Yong Feng Zhang ),( Rong De Jin ),( Si Yan Liu ),( Ro Dong Park ) 한국키틴키토산학회 2012 한국키틴키토산학회지 Vol.17 No.1
A chitosanase-producing bacterium, Bacillus cereus D-11, was isolated from an environmental soil sample. The optimal culture condition for chitosanase production by B. cereus D-11 was 3 days of cultivation at 30 o C in a medium composed of 0.5% inoculation concentration (1.4×10 8 CFU/mL), 0.7% colloidal chitosan, 1% yeast extract and 1% NaCl at initial pH 7.0. The highest activity achieved was 7.8 U/mL. The crude enzyme preparation from Bacillus cereus D-11 degraded the chitooligomers pentamer, hexamer, and heptamer into (GlcN)2-4, but not degraded the chitooligomers less than (GlcN)4. These results indicate that B. cereus D-11 chitosanase cleaves the oligomeric chains in the endo-splitting manner and the catalytic domain of D-11 chitosanase recognizes and needs at least 5 glucosamine units for hydrolytic catalysis of the glycosidic linkages.
Preparation of Chitooligosaccharides from Chitosan using Crude Enzyme of Bacillus cereus D-11
( Xing Ai Gao ),( Yong Feng Zhang ),( Ro Dong Park ),( Xiao Huang ),( Xin Ying Zhao ),( Jiao Xie ),( Rong De Jin ) 한국응용생명화학회 2012 Journal of Applied Biological Chemistry (J. Appl. Vol.55 No.1
In order to enzymatically produce chitooligosaccharide using the crude enzyme preparation from Bacillus cereus D-11, we first studied the optimal reaction conditions. It was found that the optimal temperature for hydrolysis of chitosan was 55oC. The ratio of enzyme/substrate should not be lower than 0.13 U/mg in the reaction mixture. The enzyme activity was stable below 50oC. The products of enzymatic reaction were analyzed by both thin layer chromatography and high performance liquid chromatography. Under the appropriate condition, chitosan was hydrolyzed using the enzyme preparation. The resulting chitooligosaccharides were purified and separated by Dowex (H+) ion exchange chromatography. From 4 g soluble chitosan, 0.95 g (GlcN)2, 1.43 g (GlcN)3, and 1.18 g (GlcN)4 were recovered.
Analysis and study of compact inductive power transfer systems for EV charging
Ai, Yongle,Hu, Xiaoqi,Li, Xing,Zhang, Xin The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.5
The double-sided LCC topology provides an efficient compensation method for electric vehicle (EV) wireless charging systems. However, the existence of two compensation coils results in an electric vehicle wireless charging device with a large volume, high power consumption, and low efficiency. To solve these problems, this paper proposes a wireless charging structure in which the compensation coils are separately integrated into the transmitting and receiving coils. First, the number of turns of the transmitting coil is optimized to maximize the coupling coefficient of the transmitting coil. Secondly, to minimize the redundant coupling effect, the relative placement of the compensation coils is studied. Based on the proposed coil integration method, it is possible to ignore the redundant coupling between the compensation coils and the transmitting and receiving coils. Then, the Ansys Maxwell and Ansys Twin Builder are used to build a joint simulation circuit to construct the proposed wireless charging system. Simulation and experimental results show that the system output power is 3.09 kW with a gap of 150 mm, and that the transmission efficiency is 95.49%. In addition, the integrated solution has a high transmission efficiency in the presence of front-to-back misalignment and vertical misalignment of electric vehicles.
Identification of Specific Gene Modules in Mouse Lung Tissue Exposed to Cigarette Smoke
Xing, Yong-Hua,Zhang, Jun-Ling,Lu, Lu,Li, De-Guan,Wang, Yue-Ying,Huang, Song,Li, Cheng-Cheng,Zhang, Zhu-Bo,Li, Jian-Guo,Xu, Guo-Shun,Meng, Ai-Min Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10
Background: Exposure to cigarette may affect human health and increase risk of a wide range of diseases including pulmonary diseases, such as chronic obstructive pulmonary disease (COPD), asthma, lung fibrosis and lung cancer. However, the molecular mechanisms of pathogenesis induced by cigarettes still remain obscure even with extensive studies. With systemic view, we attempted to identify the specific gene modules that might relate to injury caused by cigarette smoke and identify hub genes for potential therapeutic targets or biomarkers from specific gene modules. Materials and Methods: The dataset GSE18344 was downloaded from the Gene Expression Omnibus (GEO) and divided into mouse cigarette smoke exposure and control groups. Subsequently, weighted gene co-expression network analysis (WGCNA) was used to construct a gene co-expression network for each group and detected specific gene modules of cigarette smoke exposure by comparison. Results: A total of ten specific gene modules were identified only in the cigarette smoke exposure group but not in the control group. Seven hub genes were identified as well, including Fip1l1, Anp32a, Acsl4, Evl, Sdc1, Arap3 and Cd52. Conclusions: Specific gene modules may provide better understanding of molecular mechanisms, and hub genes are potential candidates of therapeutic targets that may possible improve development of novel treatment approaches.