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Sobhan, Abdus,Oh, Jun Hyun,Lee, Jin Young Trans Tech Publications 2018 Applied Mechanics and Materials Vol. No.
<P>Peanut protein Ara h2 is one of the serious food allergens contributing to the allergic reaction, causing the common reasons for food-related life threating problems over the world. The objective of this study is to develop single-walled carbon nanotubes (SWCNTs) based biosensor using 1-pyrenibutanoic acid succinimidyl ester (1-PBASE) as a linker for rapid detecting peanut allergen Ara h2 in foods. The detection principal of this biosensor was based upon the binding of Ara h2 to the anti-Ara h2 on the linker-modified SWCNTs connecting gold electrode in biosensor silicon template. The measurements of each step were achieved using linear sweep voltammetry (LSV) with a potentiostat for Ara h2 detection. The application of anti-Ara h2 as pAbs on linker immobilized SWCNTs surface increased the sensor resistance values. The developed biosensor showed significant resistance response with the increase in the concentration of Ara h2 from 0 to 1000 ng/mL. Indirect enzyme-linked immunosorbent assay (ELISA) was successfully applied to confirm the specificity of antibodies using 96-well microplates. Scanning electrone microscopy (SEM) confirmed the microstructure of SWCNTs. Sensor sensitivity and specificity were tested with different peanut allergens and the detection range (1-1000 ng/mL).</P>
Sobhan, Abdus,Oh, Jun-Hyun,Park, Mi-Kyung,Lee, Jinyoung Oxford University Press 2018 Journal of AOAC International Vol.101 No.5
<B>Abstract</B><P>Background: The peanut protein Arachis hypogaea (Ara h) 6 is one of the most serious food allergens that contributes to food-related, life-threatening problems worldwide. The extremely low allergic dose demands for more selective and rapid methods for detecting Ara h 6. Objective: The goal of this study was to develop a single-walled carbon nanotube (SWCNT)-based biosensor for the rapid detection of Ara h 6 in commercial food products. Methods: The detection principle of this biosensor was based on the binding of Ara h 6 to the anti-Ara h 6 antibody (pAb) through 1-pyrenibutanoic acid succinimidyl ester. The resistance difference (ΔR) was calculated via linear sweep voltammetry using a potentiostat. Results: The ΔR increased as the Ara h 6 concentrations increased above the range of 100-107 pg/L. A specificity analysis showed that the anti-Ara h 6 pAb selectively interacted with Ara h 6 molecules in the buffer solution (pH 7.4). Conclusions: This research proposes that an SWCNT-based biosensor in self-assembly with antibodies could be an effective tool for the rapid detection of allergen proteins in food. Highlights: The developed biosensor exhibited higher sensitivity and selectivity. Application studies resulted in precise Ara h 6 detection in peanut-containing processed food.</P>
Assessment of peanut allergen Ara h1 in processed foods using a SWCNTs-based nanobiosensor
Sobhan, Abdus,Oh, Jun-Hyun,Park, Mi-Kyung,Kim, Seung Wook,Park, Chulhwan,Lee, Jinyoung Informa UK (TaylorFrancis) 2018 Bioscience, biotechnology, and biochemistry Vol.82 No.7
<P>The goals of this research were to develop a rapid single-walled carbon nanotube (SWCNT)-based biosensor and to employ it to commercial food products for Ara h1 detection. The SWCNT-based biosensor was fabricated with SWCNTs immobilized with antibody (pAb) through hybridization of 1-pyrenebutanoic acid succinimidyl ester (1-PBASE) as a linker. The resistance difference (R) was calculated by measuring linear sweep voltammetry (LSV) using a potentiostat. Resistance values increased as the concentration of Ara h1 increased over the range of 1 to 10(5)ng/L. The specific binding of anti-Ara h1 pAb to antigen including Ara h1 was confirmed by both indirect ELISA kit and biosensor assay. The biosensor was exposed to extracts prepared from commercial processed food containing peanuts, or no peanuts, and could successfully distinguish the peanut containing foods. In addition, the application of present biosensor approach documented the precise detection of Ara h1 concentrations in commercially available peanut containing foods.</P>
Abdus Sobhan,오준현,박미경,김승욱,박철환,이진영 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.1
Food allergies are global trending issues in the food industry. Peanut allergy is an especially serious problem with emerging allergen symptoms because peanuts are utilized worldwide as a processing food source. Arachis hypogaea 1 (Ara h1) is a main seed storage protein from peanut materials that derives allergic medical symptoms. In this study, a single-walled carbon nanotube (SWCNT)-based biosensor was developed to detect Ara h1. The developed biosensor utilizes an Ara h1 antibody as a receptor for the target material, SWCNTs as a signal transfer, and 1- pyrenebutanoic acid succinimidyl ester (1-PBSE) as a linker between the SWCNTs and the receptors. SWCNTs dramatically transferred the biological reaction between the antibody and the antigen into measurable signals of electrical responses. The sensor capacity of the developed SWCNT-based biosensor, including the limit of detection (LOD, 1 ng/ mL), the detection range (1-1,000 ng/mL), and the washing amounts (three times), was confirmed.