http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
임신말기 자궁내 발육상태와 자궁적출 시기가 특정질병 제어 형질전환 복제동물 생후 생존에 미치는 영향
우제석(Jae-Seok Woo),황성수(Seongsoo Hwang),오건봉(Keon Bong Oh),이휘철(Hwi-Cheul Lee),양병철(Byoung-Chul Yang),임기순(Gi-Sun Im),이명식(Myeung Sik Lee),김민규(Minkyu Kim),노환국(Whangook Nho),박수봉(Soo-Bong Park),홍성구(Sunggoo Hon 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.4
Bioorgan transgenic cloned mini pig has a problem of growth retardation in uterus during end of pregnancy so that survival rate is very low in newborn piglet. In order to support their life after birth, cesarean section of fetus with sufficient growth in uterus was tested in this study. First of all, fetus growth measured using a ultrasound scanner during pregnancy in transgenic mini pig, comparing normal pig. After 113 days for delivering, fetus was removed out of uterus. Fetus growth for normal pig was 1.8 cm at 4weeks and 14.4 cm at end of pregnancy (15 weeks). At 113 days, fetus growth was 15.9±4 cm in ultrasound scanner and real growth measurement from fetus removal out of uterus was 16.0±2 cm. It is very a similar result between measurement of ultrasound scanner and real measurement. Therefore, using ultrasound scanner for measuring fetus growth will be useful to predict fetus growth in uterus.
이종이식에 활용할 α1,3-galactosyltransferase 비활성화 및 Membrane Cofactor Protein 발현 동형접합 형질전환 돼지 개발
이건섭,박상현,이해선,지수정,이주영,변승준,황성수,김경운,옥선아,오건봉,Lee, Gunsup,Park, Sang Hyoun,Lee, Haesun,Ji, Soo-Jeong,Lee, Joo Yung,Byun, Sung-June,Hwang, Seongsoo,Kim, Kyung Woon,Ock, Sun A,Oh, Keon Bong 한국수정란이식학회 2017 한국동물생명공학회지 Vol.32 No.3
Transplantation is considered to be a very useful approach to improve human welfare and to prolong life-span. Heterologous organ transplantation using pig organs which are similar to human beings and easy to make mass-production has known as one of the alternatives. To ensure potential usage of the pig organ for transplantation application, it is essentially required to generate transgenic pig modifying immuno-related genes. Previously, we reported production of heterozygous ${\alpha}1,3$-galactosyltransferase (GalT) knock-out and human membrane cofactor protein (MCP) expressing pig ($GalT^{-MCP/+}$), which is enforced for suppression of hyperacute and acute immunological rejection. In this study, we reported generation of homozygous pig ($GalT^{-MCP/-MCP}$) by crossbreeding $GalT^{-MCP/+}$ pigs. Two female founders gave birth to six of $GalT^{-MCP/-MCP}$, and seven $GalT^{-MCP/+}$ pigs. We performed quantitative real-time PCR, western blot, and flow cytometry analyses to confirm GalT and MCP expression. We showed that fibroblasts of the $GalT^{-MCP/-MCP}$ pig do not express GalT and its product Gal antigen, while efficiently express MCP. We also showed no expression of GalT, otherwise expression of MCP at heart, kidney, liver and pancreas of transgenic pig. Taken together, we suggest that the $GalT^{-MCP/-MCP}$ pig is a useful candidate to apply xenotransplantation study.
돼지에서 TRAF4 유전자 특성 및 Tight junction 관련 기능 분석
윤정희(Jeong-hee Yun),황인설(In-Sul Hwang),황성수(Seongsoo Hwang),박미령(Mi-Ryung Park) 한국산학기술학회 2020 한국산학기술학회논문지 Vol.21 No.5
Tumor necrosis factor receptor associated factor 4 (TRAF4)는 사람의 유방암에서 과발현 되며, 암세포전이, ROS 및 세포 극성 형성 등에 관여하는 것으로 알려져 있다. 그러나 돼지에서는 아직까지 그 기능과 특성에 대한 연구가 보고 된 바 없다. 따라서 본 연구에서는 돼지 TRAF4의 mRNA 전장서열을 분석하고, 그 기능과 특성을 알아보고자 수행되었다. TRAF4의 전장서열을 밝히기 위해 돼지 신장유래세포(pK15)에서 total RNA 추출하여 RACE (Rapid Amplification of cDNA ends) PCR을 수행하였다. 2,030 염기쌍의 mRNA 전장서열을 분석하였고, 470개의 아미노산으로 구성 되어 있는 것을 확인하였다. 사람과 쥐의 Homology를 분석한 결과 각각 93 % 그리고 90 %의 유사도를 가지며, 사람과는 8개, 쥐와는 12개의 아미노산 차이가 있음을 확인하였다. qPCR을 통하여 TRAF4, CLDN4, OCLN 그리고 TJP1의 발현을 분석한 결과 세포의 confluency 정도에 따라 발현이 다르게 나타남을 확인하였고, 세포가 40% 증식한 그룹 보다 60 %와 80 % 이상 증식 한 그룹에서 유의적으로 높게 나타났다. 또한 TRAF4의 기능을 확인하기 위하여 TRAF4 siRNA 처리 한 결과 TRAF4와 tight junction 관련 유전자가 낮게 발현됨을 관찰하였다. 따라서 사람과 마우스와 같이 돼지에서도 TRAF4가 발현되며, 세포-세포 간 중요한 역할을 하는 tight junction에 관여하는 것으로 사료된다. Tumor necrosis factor receptor associated factor 4 (TRAF4) is found to be overexpressed in human breast cancer. It plays a role in cancer metastasis, production of reactive oxygen species, and cell polarity at membranes. The characteristics and functions of TRAF4 in pigs have not yet been identified. As the first step of research, the mRNA sequence of TRAF4 in porcine cells has been determined. To obtain the full-length sequence, rapid amplification of cDNA ends (RACE) has been carried out. Upon cloning, 2,030 bp of nucleotides were found to encode 470 amino acids, and 8 and 12 amino acids were different from those of the human and mouse TRAF4, respectively. The coding region of porcine TRAF4 was shown to be 93% and 90% homologous to human and mouse TRAF4, respectively. qPCR was conducted to determine the relative expression level of TRAF4. TRAF4 expression in pK15 was enhanced by cell-cell contacts. The mRNA levels of CLDN4, OCLN, and TJP1 at 60% and 80% confluency were significantly higher than at 40% confluency. Further, TRAF4 and tight junction-related genes were down-regulated upon treatment with TRAF4 siRNA. Thus, TRAF4 may affect the function of tight junctions in pig.