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A New Pyrrole Constituent from the Fruits of Lycium chinense
진영원,E Ray Kim,Young-Won Chin,김진웅 한국생약학회 2011 Natural Product Sciences Vol.17 No.3
Phytochemical investigation of Lycium chinense fruits led to isolation of a new pyrrole compound. The structure of this compound was confirmed as a 5-methoxymethyl-1H-pyrrole-2-carbaaldehyde, a new natural product, by interpretation of 1D (1H, 13C) and 2D (HMQC, HMBC) spectroscopic data along with HRMS and IR spectroscopic data.
초임계 이산화탄소를 이용한 약용식물 성분의 선택적 추출
최영해,박은정,김영림,진영원,전성호,정승남,유기풍,김진웅,Choi, Young-Hae,Park, Eun-Jung,Kim, Young-Leem,Chin, Young-Won,Jeon, Seong-Ho,Joung, Seung-Nam,Yoo, Ki-Pung,Kim, Jin-Woong 한국생약학회 1999 생약학회지 Vol.30 No.1
Supercritical fluid extraction (SFE) technique was applied to extract cytotoxic substances from five medicinal plants including Angelica gigas, Angelica acutiloba, Aralia cordata, Spirodela polyrhiza, Bupleurum falcatum, and Acanthopanax sessiliflorus. The cytotoxicities against P388, A549, and HL-60 cell lines were determined for the supercritical carbon dioxide extracts of five plant materials employed and were compared with those of the conventional organic solvent extracts such as n-hexane, $CHCl_{3}$, and MeOH to evaluate the SFE as an alternative method to conventional organic solvent extraction. In most cases, the SFE extracts of plant materials showed enhanced cytotoxicities when compared with those of other organic solvent extracts. In addition, the optimum temperature and pressure of SFE for extraction of the cytotoxic substances were largely affected by both the plant species and the cell lines tested. These results suggested that SFE could be an alternative to the conventional organic solvent method for the selective extraction of cytotoxic compounds from plants.
혼합추출 및 개별추출 방식의 삼정환의 항산화 및 항비만효과
한경선 ( Kyung Sun Han ),왕경화 ( Jing Hwa Wang ),임동우 ( Dong Woo Lim ),진영원 ( Young Won Chin ),최영희 ( Young Hee Choi ),최한석 ( Han Seok Choi ),이명종 ( Myeong Jong Lee ),김호준 ( Ho Jun Kim ) 한방비만학회 2014 한방비만학회지 Vol.14 No.2
Objectives: This study is to confirm the effect of combined extract and individual extract of Samjunghwan (SJH) in anti-oxidative and anti-obesity effect. Methods: Combined ethanol extract of readily made SJH and individual ethanol extract of Atractylodes japonica, Cortex lycii radicis, and Morus alba Linne was combined after the extraction. To evaluate the anti-oxidative effect of SJH, total phenol compound and2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability were conducted. Real-time quantitative-polymerase chain reaction analysis of transcription factor peroxisomeproliferator-activated receptror γ (PPARγ), adenosine monophosphate-activated protein kinase(AMPK)-α1, tumor necrosis factor-α (TNFα) and 3-hydroxy-3-methylglutaryl CoA reducatase(HMG-CoA reductase) were done with 3T3-L1 cells to investigate the ant-obesity effect. Also,cell viability analysis were done to see to toxicity of SJH.Results: Individual extract of SJH showed significant decrease in TNFα and AMPK transcription while PPARγ showed significant increase. Combined extract and individual extract of SJH both showed decrease in HMG-CoA reductase. DPPH free radical scavenging ability and total phenolcompound was analogous between two groups. Conclusions: Individual extract of SJH appears to be more effective in anti-oxidation andanti-obesity effect compared to combined extract of SJH.
RAW 264.7 세포에서 Metformin과 병행투여 시 상승효과를 나타내는 한약재의 선별 연구
김형구 ( Hyung Gu Kim ),왕경화 ( Jing Hua Wang ),채희성 ( Hee Sung Chae ),진영원 ( Young Won Chin ),최한석 ( Han Seok Choi ),김호준 ( Ho Jun Kim ) 한방비만학회 2014 한방비만학회지 Vol.14 No.1
This study was performed to evaluate the effects of Metformin and Lonicerae Flos, Agrobacterium Rhizogenes, Coptidis Rhizoma, Atractylodis Rhizoma Alba, Houttuyniae Herba extracs combinations on hypoglycemia in RAW 264.7 cells. Methods: Expressions of Sirt1, p-adenosine monophosphate-activated kinase (p-AMPK), AMPK- alpha, peroxisome proliferator activated receptor (PPAR)-alpha, PPAR-gamma, X-box binding protein 1 (XBP-1), tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were analyzed by real time polymerase chain reaction and Western blotting analysis. Results: The level of gene expression of Sirt1, p-AMPK, AMPK-alpha, PPAR-alpha and XBP-1 in relation to that of beta-actin were increased or decreased significantly with the Metformin and Lonicerae Flos, Agrobacterium Rhizogenes extracts combination groups. The level of gene expression of TNF-alpha and IL-6 were increased significantly with the Metformin and Houttuyniae Herba, Coptidis Rhizoma extracts combination groups. Conclusions: Metformin and Lonicerae Flos, Agrobacterium Rhizogenes extracts combination groups showed synergistic hypoglycemic effects by increasing AMPK and PPAR gene expression in RAW 264.7 cells.
백출, 어성초, 금은화, 황금, 황련과 Metformin의 병용 투여 시 항산화 효과 및 전지방세포 분화 억제 효과
장세주 ( Seju Chang ),한경선 ( Kyungsun Han ),왕경화 ( Jing Hua Wang ),채희성 ( Hee Sung Chae ),최영희 ( Yong Hee Choi ),진영원 ( Young Won Chin ),최한석 ( Han Seok Choi ),김호준 ( Hojun Kim ) 한방비만학회 2015 한방비만학회지 Vol.15 No.1
Objectives: This study was to investigate the antioxiative capacity, antiobesity effects of Atractylodes Rhizoma Alba, Houttuyniae Herba, Lonicerae Flos, Agrobacterium Rhizogenes, and Coptidis Rhizoma on Raw 264.7 and 3T3-L1 cell lines. Methods: Three different types of herb extracts (A. Rhizoma Alba, H. Herba, L. Flos, A. Rhizogenes, and C. Rhizoma; water 100%, ethanol 30%, ethanol 100%) were used in this study. Total polyphenol compound, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reactive oxygen species (ROS) activity, NO production and cell proliferation were measured. Results: Total polyphenol compound measurement of L. Flos, A. Rhizogenes, and C. Rhizoma extracts were higher than A. Rhizoma Alba, H. Herba. DPPH radical scavenging activity, ROS activity and NO production of A. Rhizogenes, C. Rhizoma extracts were lower than L. Flos, A. Rhizoma, and H. Herba. Conclusions: Metformin and A. Rhizogenes, C. Rhizoma, A. Rhizoma Alba, and L. Flos extracts combination groups showed synergistic effect on adipocyte differentiation inhibition and antioxidative activity.
상심자와 건조상심자 추출물의 항산화 효과 및 전지방세포 분화억제 효과에 관한 연구
김형구 ( Hyung Gu Kim ),왕경화 ( Jing Hua Wang ),임동우 ( Dong Woo Lim ),채희성 ( Hee Sung Chae ),진영원 ( Young Won Chin ),최한석 ( Han Seok Choi ),김호준 ( Ho Jun Kim ) 한방재활의학과학회 2014 한방재활의학과학회지 Vol.24 No.4
ObjectivesThis study was to investigate the antioxidative capacity, antiobesity effect and anti-diabetes effects of Mori Fructus and dried Mori Fructus in Raw 264.7 cells and 3T3-L1 cells.Methods3 different types of Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. And 3 different types of dried Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. Total polyphenol compund, total favonoid compound, DPPH radical scavenging, ROS activity, NO, cell proliferation were measured in the experiment. Expressions of adipogenic transcription factors including C/EBP-α, C/EBP-β, PPAR-α, PPAR-γ, AMPK-α were analyzed by Real time PCR. ResultsMori Fructus extracts measurements are higher than dried Mori Fructus extracts measurements at Total flavonoid compound and total flavonoid compound. Mori Fructusextracts measurements are lower than dried Mori Fructus extracts measurements at DPPH radical scavenging, ROS activity, NO. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts inhibit the expression of C/EBP-α, C/EBP-β genes. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts promote the expression of PPAR-α, PPAR-γ, AMPK-α genes. ConclusionsMori Fructus is effective on inhibiting the oxidation and dried Mori Fructus is effective on inhibiting the obesity and diabetes. (J Korean Med Rehab 2014;24(4):1-13)