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정달상,김철원,Jeong, Dal Sang,Kim, Chul Won 국립한국농수산대학교 교육개발센터 2014 현장농업연구지 = Journal of practical agricultural resear Vol.16 No.1
Olive flounder (Paralichthys olivaceus) has a great potential value for aquaculture in Korea. The theoretical sex ratio of this flounder is close to 1:1 as it is in many other type of fish. However, according to the size selection during artificial seedling production, there is a possibility of unbalanced sex ratio. The actual flounder culturing needs female seedlings because of faster growth than male in economically. However, little is known about the sex ratio of cultured flounder. The six populations consisted of three wild populations and three cultured populations were analyzed for investigating the sex ratio. The sex ratio of wild populations ranged from 1.10 to 1.93 in female and 0.52 to 0.91 in male. And the sex ratio of cultured populations ranged from 0.20 to 2.15 in female and 0.46 to 4.88 in male. This result indicated that the sex ratio of cultured flounder varies depending on the size selection method.
감성돔(Acanthopagrus schlegelii) 치어의 포르말린에 대한 내성 및 조직학적 반응
명정인 ( Jeong In Myeong ),민병화 ( Byunng Hwa Min ),박미선 ( Mi Seon Park ),황형규 ( Hyung Kyu Hwang ),도정완 ( Jeung Wan Do ),정경일 ( Kyung Il Jeoung ),장영진 ( Young Jin Chang ),정달상 ( Dal Sang Jeong ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.6
Black seabream juveniles Acanthopagrus schlegelii held at 20°C were exposed to formalin at concentrations of 50 to 400 ppm, and tolerance values were determined by calculating median lethal concentration values (LC50) through probit analysis. The 12-, 24,48, 72- and 96-h LC50 values for formalin were 297, 233, 171, 162 and 157 ppm, respectively. The histological effects of formalin on gill and liver tissues in this fish were determined. No histological effects were observed in the control group. The intensity of cell damage increased with the concentration of, and duration of exposure to, formalin. Hyperplasia, separation and epithelial necrosis,epithelial lifting, lamellar synechiae and collapsed lamellae were observed in gill tissues exposed to formalin. Hepatic lesions in liver tissues of fishes exposed to formalin were characterized by cloudy swelling of hepatocytes, necrosis, cytoplasmic vacuolization,deposition of pigments, spongiosis hepatis, nuclear hypertrophy, dilation of sinusoids and bile stagnation. The LC50 values and histological results obtained in this study will aid in designing treatment regimens to minimize toxic side effects and increase efficacy.
수온 변화에 따른 방사무늬김(Neopyropia yezoensis) 패각 사상체의 각포자 형성량 비교
이은택,정달상,김철원,최성제,Eun Taek Lee,Dal Sang Jeong,Chul Won Kim,Sung Je Choi 국립한국농수산대학교 교육개발센터 2023 현장농업연구지 = Journal of practical agricultural resear Vol.25 No.3
This study investigated effect of water temperature change on the formation and release of conchospores of Neopyropia yezoensis. We observed that conchocelis growth and conchospores formation in oyster shell at labolatory during 7 weeks. In order to investigate the amount of conchospore formation in oyster shells, which was being cultured at 28℃, was moved to 10℃, 18℃, 28℃, and culture during 6 weeks. At 10℃, we observed an average of 127 for 1 week, 127 for 2 weeks, 95 for 3 weeks, 90 for 4 weeks, 76 for 5 weeks, and 75 for 6 weeks. At 18℃, we observed an average of 141 for 1 week, 135 for 2 weeks, 94 for 3 weeks, 153 for 4 weeks, 162 for 5 weeks, and 2 for 6 weeks. At 28℃, we observed an average of 167 for 1 week, 102 for 2 weeks, 148 for 3 weeks, 157 for 4 weeks, 270 for 5 weeks, and 138 for 6 weeks. Conchospores released from the shell grew into a young thalli in the culture for 6 weeks, and the number of ones was counted. The number of young thalli were investigated at 10℃, 0 for 1 week, 189 for 2 weeks, 200 for 3 weeks, 89 for 4 weeks, 56 for 5 weeks and 27 for 6 weeks. At 18℃, It observed 0 for 1 week, 26 for 2 weeks, 546 for 3 weeks, 16 for 4 weeks, 17 for 5 weeks and 154 for 6 weeks. It was not observed at 28℃.
크롬 노출에 따른 숭어, Mugil cephalus의 혈액성분 변화
안태영 ( Tae Young Ahn ),정달상 ( Jeong Dal Sang ),김준환 ( Jun Hwan Kim ),강주찬 ( Ju Chan Kang ) 한국어병학회 2013 한국어병학회지 Vol.26 No.2
크롬은 여러 산업으로 배출되어 수 환경을 오염시키는 주요 중금속 중 하나이며, 특히 Cr(Ⅵ)은 수용액 내에서 주로 크롬산 이온으로 존재하며, 생체막을 쉽게 통과하여 산화 스트레스 및 생체 축적을 일으킨다. 따라서 본 연구는 숭어를 대상으로 그들의 혈액성분의 변동에 미치는 크롬의 영향을 검토하였다. RBC count 및 Ht 값은 50 ㎍/L Cr 농도에서 유의적인 감소를 나타냈으나, Hb 농도의 유의한 변동은 관찰되지 않았다. 혈청 칼슘농도는 50 ㎍/L Cr의 농도에서 유의적인 감소를 보였고, 마그네슘농도는 100 ㎍/L Cr의 농도에서 유의적인 증가를 보였다. Glucose 및 total protein 농도는 가장 높은 크롬농도인 200㎍/L Cr에서 유의적인 증가를 보였으나, 중성지방인 triglyceride 농도는 노출기간 동안 유의한 변화가 관찰되지 않았다. GOT 및 GPT 활성은 100 ㎍/L Cr 이상의 농도에서 유의적인 증가를 보였고, ALP활성은 가장 높은 크롬농도인 200 ㎍/L Cr에서 유의적인 증가를 보였다. 이상의 결과는 숭어의 혈액성상과 혈청 무기성분은 크롬 농도 50 ㎍/L 이상, 혈청유기성 분과 효소성분은 크롬농도 100 ㎍/L이상에서 변동이 나타나는 것으로 결론 내릴 수 있다. The study was carried out to investigate the changes of hematological parameters induced by waterborne exposure of chromium (Cr) in the mullet, Mugil cephalus. The mullet was exposed to sub-chronic concentrations of chromium (0, 25, 50, 100, 200 ㎍/L Cr) for 4 weeks. The major hematological findings were significant decreases in the red blood cell (RBC) count and hematocrit value in mullet exposed to ≥50 ㎍/L Cr. Although serum calcium concentration was significantly reduced at ≥50 ㎍/L Cr, magnesium concentration was found to be significantly increased at ≥100 ㎍/L Cr. The serum glucose and total protein concentrations were significantly increased at 200 ㎍/L Cr. However, serum triglyceride concentration did not show any noticeable changes in the range of 25∼200 ㎍/L Cr of chromium compared to control group during the experimental period. A significant increment of GOT (glutamic oxalate transminase) and GPT (glutamic pyruvate transminase) activities was noticed at ≥100 ㎍/L Cr. These results indicate that hematological and serum biochemical changes in the mullet by waterborne exposure to chromium are affected at more than 50 ㎍/L Cr.