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대장암에서 CD31과 D2-40 발현에 의한 혈관성 및 림프관성 미세혈관 밀도 측정
윤상인(Sang In Youn),김범규(Beom Gyu Kim),차성재(Seong Jae Cha),박성준(Sung Jun Park),장인택(In Taek Chang),박성일(Sung Il Park),이태진(Tae Jin Lee) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.73 No.4
Purpose: Angiogenesis and lymphangiogenesis play important roles in the growth, progression and metastasis of colon cancer. We performed this study in order to investigate the significance of the CD31 and D2-40 expressions as prognostic factors in colon cancer. Methods: The angiogenic and lymphagiogenic microvessel density was assessed by immunohistochemical staining of CD31 and D2-40 on samples that were resected from 66 patients with colorectal cancer. Results: Strong correlation was observed between the CD31 microvessel density and being positive for having tumor emboli and lymph node metastasis (P=0.001, P=0.003). The D2-40 lymphatic vessel density was correlated with being positive for having tumor emboli (P=0.0001), the depth of invasion (P=0.0001), lymph node metastasis (P= 0.0001) and the cancer stage (P=0.0001). The D2-40 lymphatic vessel density was also correlated with the CD31 count (P=0.003). Conclusion: These results suggested that the CD31 and D2-40 expressions are useful predictors of lymph node metastasis and they are prognostic factors for colon cancer.
대장직장암에서 수술 전 생검 조직의 범내피세포와 림프관 표지자 발현의 의의
한규성(Gue Sung Han),김범규(Beom Gyu Kim),차성재(Seong Jae Cha),장인택(In Taek Chang),이태진(Tae Jin Lee) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.73 No.2
Purpose: Panendothelial markers such as factor Ⅷ, CD34, CD31, CD105 (endoglin) and D2-40 are useful to identify proliferating endothelium that is related to tumor invasion. This study was designed to identify the correlation between the expressions of panendothelial and lymphatic vessel markers in preoperative biopsy specimens and the clinicopathologic factors. Methods: Preoperative biopsy specimens from 72 patients were immunostained for CD105, CD34, CD31, Factor Ⅷ and D2-40. The microvessel and lympathic vessel densities (MVD and LVD) were counted in dense vascular foci (hot spots) on a ×200 field in each specimen. The correlation between these factors and the clinicopathologic parameters were analyzed. Results: The MVD by CD105 showed statistically significant correlation with tumor emboli, the T-stage, nodal metastasis and the stage, and the MVD by CD34 had statistically significant correlation with tumor emboli, nodal metastasis and the stage. The lympathic vessel density (LVD) by D2-40 showed a statistically significant correlation with tumor emboli, the T-stage and nodal metastasis. Conclusion: The MVD by CD105 and the LVD by D2-40 in preoperative biopsy specimens of colorectal cancers may be useful markers for the prediction of invasiveness.
김성수(Sungsoo Kim),이태진(Tae Jin Lee),김범규(Beom Kyu Kim),차성재(Sung Jae Cha),박성준(Sung Jun Park),장인택(In Taek Chang),박성일(Sung Il Park) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.72 No.5
Purpose: CD105 (endoglin) has been shown to be a more useful marker to identify the proliferating endothelium involved in tumor angiogenesis than are the panendothelial markers. The monoclonal antibody D2-40 is a specific lymphatic endothelial marker. Methods: We investigated CD105, lymphatic vessel marker (D2-40), vascular endothelial growth factor (VEGD)-A and the VEGF-D expressions as possible prognostic markers in the endoscopic biopsy tissue of stomach cancer patients. The pre-operative endoscopic biopsies and surgical biopsies from 73 patients were immunostained for CD105, D2-40, VEGF-A and VEGF-D. Positively stained microvessels were counted in densely vascular foci (hot spots) at a ×200 field in each specimen. Results: The microvessel density (MVD) and lymphatic vessel density (LVD), according to the CD105 and D2-40 expressions of the endoscopic biopsies, showed a statistically significant correlation with the surgical biopsies. The MVD via CD105 a showed statistically significant correlation with the histologic differentiation, T-stage, nodal metastasis and stage in the endoscopic biopsies and surgical biopsies, respectively. The lympathic vessel density (LVD) via D2-40 showed a statistically significant correlation with T-stage, nodal metastasis and stage in the endoscopic biopsies. The expressions of VEGF-A and VEGF-D showed a statistically significant correlation with the MVD and LVD. Conclusion: The MVD, as determined by the CD105 expression and the LVD as determined by the D2-40 expression may be useful markers for predicting the invasiveness with using a pre-operative endoscopic biopsy of stomach cancer.
Staphylococcus aureus α-toxin과 용연구균 간의 인혈액 배지에서의 상승 용혈반응의 민감성에 관한 연구
장인택,최철순,양용태 중앙대학교 의과대학 의과학연구소 1981 中央醫大誌 Vol.6 No.4
Recently, a new synergistic hemolysis between S. aureus α-toxin and factors of group A β-hemolytic streptococci, similar to CAMP phenomenon observed between S. aureus β-toxin and group B of streptococci, has been demonstrated (Choi and Yang, 1980). This phenomenon appeared to be one of useful presumptive tests for the primary differentiation of group A streptococci from other groups of β-hemolytic streptococci encountered in human infections as the CAMP test for group B streptococci. However, this new synergistic hemolysis did have a disadvantage that approximately 80%, of group A streptococci gave positive synergistic hemolysis reaction on sheep blood agar but only rarely the strains gave the synergistic hemolysis reaction on human blood agar (Lee et al., 1981). In this study, a sensitivity of the new synergistic hemolysis on human blood agar was studied. An attempt was made to enhance the production of streptococcal factors by the addition of dextrose, sucrose or lactose to human blood agar under the aerobic and microanaerobic (candle jar culture) conditions. Also the effect of each one of these three sugars on the production of α-toxin of S. aureus was investigated. In addition, a comparative sensitivity of synergistic hemolysis reaction, bacitracin susceptibility test and colony inhibition test was evaluated with group A β-hemolytic streptococci. The results are summarized as follows: The addition of dextrose, an inhibitor of streptolysin S, to human blood agar markdely inhibited the hemolytic activity and retarded the growth of colonies of group A streptococci, whereas both sucrose and lactose apparently retarded the growth of colonies but only slightly lowered the hemolytic activity of them. The production of α-toxin of S. aureus was markdely enhanced on the media supplemented with either 1%, dextrose or sucrose, and α-toxin production was further augmented by candle jar culture system, The sensitivity of the synergistic hemolysis reaction was much higer in candle jar culture system than in conventional culture system. However, the addition of one of three sugars to the human blood agar medium completely abolished the synergistic hemolysis reaction. Of 41 strains of group A streptococci tested, 39 strains (98.%) were bacitracin susceptible, 38 strains (92.6%) were sensitive to colony inhibition by dextrose, and 40 strains(97.5%) were positive in synergistic hemolysis reaction. All of group A streptococci that exhibited the synergistic hemolysis reaction were characterised by "crescent-shaped" synergistic hemolysis, while two of 5 strains of group B streptococci gave 'arrowhead-shaped' synergistic hemolysis. All of the other groups of streptococci tested were negative in these three parameters. This study clearly indicated that the microanaerobic (candle jar culture) condition is essential for the enhancement of the synergistic hemolysis reaction between S. aureus α-toxin and factors of group A β-hemolytic streptococci, and that an advantage of the dual test with the synergistic hemolysis test and bacitracin susceptibility test over the single test with bacitracin test in the presumptive. identification of group A streptococci.