수종의 구아닌 유사체의 DNA 절단작용에 대한 pH와 이온의 영향

이진하,최종미,김금수 ( Jin Ha Lee,Jong Myee Choi,Geum Soo Kim ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2

The physiological function of some guanine analogs separated in the reaction of guanine related compounds with 2,3-dihydroxy-2-propenal on double strand DNA was investigated. The reaction of the analogs with calf thymus DNA was carried out in various ranges of pH and in each buffer solution with different ionic strengths. The DNA-breaking action of the compounds was affected by pH, ionic strengths and species of ions.

군산·장항 해안지역 해수 , 해안저질토 및 어패류 중 중금속함량

이진하,한강완,조재영 ( Jin Ha Lee,Kang Wan Han,Jae Young Cho ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.4

Contents of heavy metals in sea waters, sediments, fishes, and shellfishes from Kunsan·Changhang coastal areas were investigated. Average contents of heavy metals in sea water were Pb 2.35, Cd 0.52, Cu 2.09, Zn 13.65 and Hg 0.24 ㎍/ℓ. Average contents of heavy metals in sediments were Pb 19.49, Cd 0.15, Cu 9.31, Zn 20.07 ㎎/㎏, and Hg 13.6 ㎍/㎏. Average contents of Pb, Cd, Cu, Zn, and Hg in Acantogobius hasta were 1.08, 0.05, 2.24, 58.14, and 0.151 ㎎/㎏ and those in Mugil cephalus were 1.63, 0.05, 1.51, 32.69, and 0.065 ㎎/㎏. Accumulation ratios of Pb and Cu in Acantogobius hasta were similar to Mugil cephalus but accumulation ratios of Zn and Hg in Acantogobius hasta were higher 2 times than in Mugil cephalus. Average contents of Pb, Cd, Cu, Zn, and Hg in Cycling sinensis were 1.03, 1.23, 6.83, 75.83, and 0.071 ㎎/㎏ and those in Ruditapes philippinarum were 0.93, 0.86, 5.90, 52.16, and 0.074 ㎎/㎏. Accumulation ratios of heavy metals in shellfishes were in the order of Cu$gt;Cd$gt;Zn$gt;Pb$gt;Hg, irrespective of shellfishes kind.

Effect of pH and Ionic Strengths on the DNA-Breaking Action of Some Guanine Analogs

이진하,최종미,김금수,Lee, Jin-Ha,Choi, Jong-Myee,Kim, Geum-Soo 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2

구아닌 관련 화합물과 2,3-dihydroxy-2-propenal과의 반응에서 분리된 수종의 구아닌 유사체들의 DNA에 대한 작용을 조사하였다. 각종 pH를 가진 다양한 완충용액 중에서 DNA와 이들 유사체와 반응을 행하여 이들 화합물이 pH나 이온의 강도에 따라 DNA 절단 기능이 다양하게 영향을 받는 것이 확인되었다. The physiological function of some guanine analogs separated in the reaction of guanine related compounds with 2,3-dihydroxy-2-propenal on double strand DNA was investigated. The reaction of the analogs with calf thymus DNA was carried out in various ranges of pH and in each buffer solution with different ionic strengths. The DNA-breaking action of the compounds was affected by pH, ionic strengths and species of ions.

Effect of Oxygen and Metal Ions on the DNA-Breaking Action of Some Guanine Analogs

이진하,Lee, Jin-Ha 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.4

Guanosine과 guanylic acid를 triose reductone으로 수식하여 조제(造製)한 $N^2$-(3-oxo-2-hydroxypropenyl) guanosine과 $N^2$-(3-oxo-2-hydroxypropenyl) guanylic acid의 DNA 절단작용에 대한 산소, 강환원제인 dithiothreitol, 및 수종의 금속 ion들의 영향을 agarose gel electrophoresis로 검토하였다. $Cu^{2+}$ 존재하에서 guanine 유도체 들은 그들의 산화 및 가수분해 작용에 의하여 DNA의 절단에 관여하고 있었고 질소 gas와 dithiothreitol 등은 산화 및 가수분해 작용을 방해하므로 DNA의 fragmentation을 지연 또는 저해시켰다. $Cu^{2+}$, $Pb^{2+}$, $Zn^{2+}$, $Fe^{2+}$, $Fe^{3+}$ 등의 ion 중에서, $Cu^+2$가 이 유도체들의 DNA-breaking 작용에 가장 강한 촉진역할을 하였다. The effect of oxygen, dithiothreitol and some metal ions on the DNA-breaking action of the guanine analogs, $N^2$-(3-oxo-2-hydroxypropenyl) guanosine and $N^2$-(3-oxo-2-hydroxypropenyl) guanylic acid, which were prepared by the modification of guanosine and guanylic acids with triose reductone, were investigated at $37^{\circ}C$ at physio logical pH by an agarose slab gel electrophoresis. The oxidation and the hydrolysis of the analogs were related to the fragmentation of double strand DNA but a nitrogoen gas and strong reducing reagent, dithiothreitol, interfered with the oxidation and the hydrolysis. Among the metal ions, $Cu^{2+}$, $Pb^{2+}$, $Zn^{2+}$, $Fe^{2+}$ and $Fe^{3+}$, $CuSO_4$ showed the strong accerelating effect on the DNA-breaking action of the guanine derivatives.

DNA Breaking Action of Guanine Analogs Modified by Triose Reductone

이진하,Lee, Jin-Ha 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.3

Triose reductone으로 수식된 guanine 유도체의 $\lambda$ 및 calf thymus DNA에 대한 절단작용을 agarose electrophoresis로 검토하였다. Tricylcic type인 $N^2$-(2-hydroxypropenylidene) guanin과 open type인 $N^2$-(3-oxo-2-hydroxypropenyl) guanine은 $CuSO_4$ 존재하에서 DNA 절단작용을 가지고 있으며 후자가 더 강한 활성을 가지고 있다. 또한, 그 guanine 유도체들의 DNA에 대한 절단작용은 triose reductone 보다 약한 활성을 나타냈다. Double strands breakage of guanine analogs modified by triose reductone on $\lambda$ and calf thymus DNA was investigated by using agarose slab gel electrophoresis, $N^2$-(2-hydroxypropenylidene) guanine and $N^2$-(3-oxo-2-hydroxypropenyl) guanine, possessed, the DNA breaking activity in the presence of $CuSO_4$, and the latter was more active than the tricyclic type analog. The DNA breakability of the analogs was weaker than that of triose reductone.

A Study on the Effect of Residual Stress between Body and Glaze of Pottery on the Mechanical Strength

이진하,나은상,최성철,Lee, Jin-Ha,Na, Eun-Sang,Choe, Seong-Cheol Materials Research Society of Korea 1999 한국재료학회지 Vol.9 No.2

In this study, the effect of residual stress on mechanical strength was investigated with 1 kind of whiteware body and 4 kinds of glazes which are produced in succeeding ceramic art place. Using dipping method, the body was coated for different times in order to manipulate the coating thickness and sintered in the different temperatures ($1200^{\circ}C$, $1250^{\circ}C$, $1300^{\circ}C$, $1350^{\circ}C$) for two thus hours. The sintered bodies were characterized by XRD, EPMA, FEM and UTM in order to study the forming of reaction layer between body and glazes, residual stresses and the effects of residual stresses on mechanical strength of pottery. At $1300{\circ}C$, we obtained maximum density and mechanical strength. By the finite element method, the residual stresses in surface of body were minimum in specific thickness of glazes and the mechanical strength of body in that thickness of glazes showed maximum when the firing temperature was settled.

군산 공업단지 인근 논 토양 및 수도체 중 중금속 함량

이진하,한강완,조재영 ( Jin Ha Lee,Kang Wan Han,Jae Young Cho ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.4

Contents of heavy metals in paddy soils and brown rice of near the Kunsan industrial complex area were investigated. Average total heavy metal contents in paddy soils were Pb 34.33, Cd 0.50, Cu 35.49, and Zn 71.67 ㎎/㎏ respectively. There are no correlation between distances from industrial complex and contents of heavy metals in paddy soils. Average contents of heavy metals in brown rice grown above paddy soil were Pb 1.74, Cd 0.07, Cu 3.54, and Zn 16.53 ㎎/㎏. Relationships between 0.1N HCl soluble heavy metals in paddy soils and contents of brown rice showed positive significant correlations for Pb at 1% level(r=0.6853^(**)) and for Zn at 5% level(r=0.5420^*). Relationships between total contents of heavy metals in paddy soils and contents of brown rice showed only positive significant correlation for Pb at 1% level(r=0.7131^(**)). Contents of heavy metals in paddy soils and brown rice of near the Kunsan industrial complex was polluted partly by the influence of smoke and dust out from industrial complex but not polluted anxious level.

TLC를 이용한 올리고당 각 성분 총 당량의 빠르고 정량적인 분석

이진하(Jin-Ha Lee),이형우(Hyoung-Woo Lee),이형기(Hyoung-Ki Lee),조동련(Dong-Lyun Cho),선우창신(Chang-Sin Sunwo),박기덕(Ki-Deok Park),최정식(Jeong-Shik Choi),김도원(Do-Won Kim),김도만(Doman Kim) 한국생물공학회 2004 KSBB Journal Vol.19 No.4

아마란스 안토시아닌의 안정성과 항산화 작용 조사

이진하(Jin-Ha Lee),이혜빈(Hye-Bin Lee),최선일(Sun-Il Choi),정태동(Tae-Dong Jung),조봉연(Bong-Yeon Cho),최승현(Seung-Hyun Choi),심완섭(Wan-Sup Sim),한웅호(Xionggao Han),이옥환(Ok-Hwan Lee) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.12

본 연구에서는 아마란스 안토시아닌 색소의 온도별(4, 25 및 40°C), pH별(3.0, 7.0, 및 9.0), 당에 대한 안정성을 12일간 4일 간격으로 색소 함량, 총 페놀 함량, DPPH 라디칼 소거능, 아질산염 소거능 및 FRAP를 측정하여 평가하였다. 아마란스 안토시아닌 색소는 저온 4°C과 pH 3.0에서 색소함량의 변화가 적었으며, glucose, xylose를 첨가 시 당의 첨가에 따른 색소 함량의 큰 변화는 보이지 않았으나 다른 당에 비해 fructose 첨가에 의해 색소 함량의 감소가 다소 크게 측정되었다. 아마란스의 항산화 효과를 측정한 결과 아마란스 추출물 1, 2.5, 5 mg/mL의 DPPH 라디칼 소거능은 37.67±0.87%, 57.65±0.52%, 88.09±0.10%로 평가되었고, 아질산염 소거능은 아마란스 추출물의 농도를 2.5, 5 및 10 mg/mL로 처리하였을 때 각각 61.53±0.03%, 64.54±0.46% 및 75.70±0.32%로 평가되었다. 또한, FRAP 활성은 아마란스 추출물 2.5, 5 및 10 mg/mL 농도에서 0.34±0.00, 0.40±0.01, 0.53±0.01로 추출물의 농도가 증가함에 따라 항산화 활성도 증가하였다. 이상의 결과는 향후 아마란스를 활용한 가공식품 미 천연 식품 색소 개발을 위한 기초자료로 활용할 수 있을 것으로 생각된다. This study investigated the stability of anthocyanin and antioxidant activities from amaranth baby leaf extracts. Amaranth baby leaf was examined to investigate the effects of pH, temperature, and sugars on the stability of anthocyanin. Antioxidant compounds and activities were measured based on total phenolic content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities, nitrite scavenging activity, and ferric ion reducing antioxidant power (FRAP). In the results, the stability of anthocyanin rapidly decreased at 40°C, while the stability of anthocyanin slowly decreased at 4°C. The type of sugar (glucose, fructose, xylose) did not influence the stability of anthocyanin. The stability of anthocyanin was reduced in the following order: pH 3> pH 9> pH 7.0. Total phenolic content was 5.62±0.12 mg gallic acid equivalent/g, DPPH radical scavenging activity of amaranth baby leaf extracts ranged from 37.67±0.87 to 88.09±0.10%, and FRAP value increased depending upon the concentrations of amaranth baby leaf extracts and ascorbic acid. The nitrite scavenging ability of amaranth baby leaf extracts ranged from 61.53±0.03 to 75.70±0.32%. In conclusion, amaranth baby leaf can be used as a potential source of natural antioxidant compounds and natural food colorants.

해당화 뿌리 추출물의 면역 중진 효과

이미경(Mi Kyoung Lee),이서호(Seo Ho Lee),최근표(Geun Pyo Choi),유창연(Chang Yeon Yu),이신영,이진하(Jin Ha Lee),이현용(Hyeon Yong Lee) 한국약용작물학회 2003 한국약용작물학회지 Vol.11 No.1

The biological activities of extracts from Rosa rugosae Radix were compared. About 78% of the growth of human hepato-carcinoma and 68% of human gastric cancer cell was inhibited in adding 0.5% ㎎/ml of the extracts of Rosa rugosae Radix respectively. The growth of human breast cancer cells was also inhibited in adding 0.5㎎/ml of the extracts as well as 66% of the human cancer cells. It was proved that the growth of human normal lung cell, scored as 20% for the extracts. Overall selectivity of the extracts on several human cancer cell line was over 4, which in higher than those from the Rosa rugosae Radix. The growth of both human immune B and T cells was enhanced up to 1.2 to 1.5 times by adding the extracts, compared to the controls. The secretion of tumor necrosis factor-alpha(TNF-α) from T cell was also increased up to 61.9pg/ml in adding the ethanol extract (0.5㎎/ml). Ethanol extract also increased up to about 61.3pg/ml of interleukin-6(IL-6) from B cell.