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한국에서 분리된 콕사키 바이러스 B3 cDNA의 클로닝 및 전체 염기서열 분석
정윤석(Yoon Suk Jung),김기순(Gee Sun Kim),박정구(Jung Goo Park),이윤성(Yoon Sung Lee),신수연(Soo Yeon Shin),천두성(Doo Sung Chun),지영미(Young Mi Jee),김문보(Mun Bo Kim),나병국(Byung Gook Na),윤재득(Jae Deuk Yoon),이광호(Gwang Ho Lee 대한바이러스학회 2000 Journal of Bacteriology and Virology Vol.30 No.1
We have determined and analyzed the full-length cDNA sequence of a coxsackievirus B3 (CVB3) Korean isolate (CVB3-Korea/97) which has been known as a general human pathogen. The whole genome contains 7,400 nucleotides and has a single large open reading frame with 6,555 nucleotides that encodes a potential polyprotein precursor of 2,185 amino acids. The genome also contains a 5 non-coding region (NCR) of 741 bases and a 3' NCR of 104 bases followed by poly(A) tail. Sequence homologies of nucleotides and deduced amino acids between the CVB3-Korea/97 strain and the prototype (Nancy strain) were 81.7% and 91.5%, respectively. The genes encoding the functional proteins including viral protease and RNA dependent RNA polymerase showed higher homology than those encoding the structural proteins. We have further analyzed the sequences of 5' NCR, VP1 and VP2 of CVB3-Korea/97, which are known as cardiovirulent determining factors at the nucleotide and amino acid levels. Although the CVB 3-Korea/97 strain was isolated from an aseptic meningitis patient without cardiomyopathy, its 234th nucleotide and 165th amino acid were uracil and Asn as same as those of other cardiovirulent strains one. However, the 155th amino acid of VP1, which closely associated with cardiovirulence, was replaced with Arg by single nucleotide substitution from A2916 to T2916. Moreover, additional amino acid substitutions were observed in the flanking region of Asp155. Taken together, amino acid(s) substitution in VP1 may play a critical role in determining cardiovirulence of the CVB3- Korea/97 strain rather than individual nucleotide replacements in the 5' NCR and/or an amino acid substitution in VP2.
PMSG 기반의 풍력 에너지 변환 시스템의 Flying Start를 위한 가상 저항 선정 방식
이윤성(Yoon-Seong Lee),추경민(Kyung-Min Choo),정원상(Won-Sang Jeong),김진욱(Jin-Wook Kim),이준신(Junsin Yi),원충연(Chung-Yuen Won) 한국조명·전기설비학회 2021 한국조명·전기설비학회 학술대회논문집 Vol.2021 No.11
In this paper a virtual resistance selection for flying start of a PMSG-based wind energy conversion system(WECS) was proposed. In the WECS, when the wind turbine rotates at a certain speed or more, the power converter start to generating. Therefore, when sensorless is applied, a technique for estimating the correct rotor speed and position is required. The virtual resistance is applied to estimate the speed and position of the rotor, and the flying start of the sensorless PMSG-based wind energy conversion system is performed. The validity of the proposed method was verified through PSIM simulation.