치주조직 유관세포에서의 Nitric Oxide 및 Nitric Oxide Synthetase의 생물학적 특성에 관한 연구

윤형진,윤동환,유형근,신형식,Yoon, Hyung-Jin,Yoon, Dong-Whan,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 1997 Journal of Periodontal & Implant Science Vol.27 No.4

Bone remodeling is characterized by the coupling of osteoclast-mediated bone resorption and osteoblast-mediated bone formation. The process is tightly regualted at the local level by an incompletely known netwotk of peptide and non-peptide fators. Nitric oxide(NO), synthesized by nitric oxide synthetase(NOS) from L-arginine, is becoming recognized as an important bioregualtory molecule in a variety of tissue, but little is known about its possible role in periodontal tissue. The purpose of this study is to investigate the expression of nitric oxide synthetase(NOS) in inflamed gingiva and the effects of cytokine on the expression of NOS protein. The expression of NOS in gingival tissue was evaluated by immunohistochemical staining for $NOS_1$, $NOS_2$, $NOS_3$. The effect of cytokine on the expression of NOS in human periodontal ligament cells and osteoblast-like HOS cells by western blot analysis. Further, we studied that NO functions in periodontal ligament cells as a regulatory molecule. PDL cells incubated with NOS inhibitor and donor. The protein expression, type I collagen & non-collagenous protein, nitrate production and cell proliferation were evaluated The results were as follows. 1. $NOS_1$, $NOS_2$, $NOS_3$ was rarely distributed in healthy gingiva, but stronger stained in gingival epithelium, endothelial cells, and mononuclear cells of inflammed gingiva. 2. The cytokine stimulated $NOS_1$, and $NOS_3$ protein were not inducing or inhibitory effect to compared with control in PDL and HOS cells. 3.Incubation of cells with combination of $TNF-{\alpha}$, $IFN-{\gamma}$, LPS result in a time dependant increase in $NOS_2$ expression, reaching a maximal level after 24 hours of stimulation. 4. The osteonectin protein inhibitory effect of NMA, inhibitor of NOS, was reversed by Larginine in dose dependant manner. 5. NMA decreased cell poliferation and nitrate production, but the inhibitory efffect of NMA was also prevented by the NO donor, sodium nitropruiside. These results suggest that exogenously synthesized NO was playing a stimulating effect on cell proliferation or on non-collagenous protein expression. Therefore NO have an important role in mediation of localized bone destruction associated inflammatory bone disease such as periodontitis.

Chlorhexidine용액 구강양치와 치은연상치태 제거의 임상 및 미생물학적 변화에 대한 연구

윤형진,강현구,신형식,Yoon, Hyung-Jin,Kang, Hyun-Koo,Shin, Hyung-Shik 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.2

The purpose of this study was to assess the clinical and microbiological effect of chlorhexidine rinse and supragingival plaque control following scaling and root planing on adult periodontal disease. 14 patients with adult periodontitis were selected for the study . They had not taken antibiotics for 6 months and history of dental treatment for 6 months before the study. Patients received a supragingival scaling and root planing under local anesthesia, plaque control group was subjected to professional plaque control 2 times for a period 2 week, chlorhexidine rinse group were subjected to twice daily 0.2% chlorhexidine rinse for a period 2 week. Clinical examination (plaque index, gingival index, probing pocket depth) and distribution of the bacteria morphology of subgingival plaque were monitored on baseline (0 week), 1 week, 2 week, 4 week and 6 week. The results were as follows : 1. Plaque index in chlorhexidine rinse group , plaque control group and control group was significantly reduced during all weeks (P<0.05). 2. Probing pocket depth was significantly reduced at 2, 4, 6 week (P<0.05) in chlorhexidine rinse group and control group, plaque control group was significantly reduced during all weeks (P<0.05). 3. Gingival index was significantly improved at 2, 4, 6 weeks(P<0.05) in chlorhexidine group and plaque control group, control group was significantly improved at 1, 2, 4 weeks (P<0.05). 4. Percentage of cocci was significantly increased at 1, 2, 4 and 6 weeks in chlorhexidine rinse group and control group, plaque control group was significantly increased at 2, 4 and 6 weeks(P<0.05). 5. Percentage of non-motile rods in all group were not significantly changed when compared with those of baseline (0 week) (P<0.05). 6. Percentage of motile rods was significantly reduced during all weeks (P<0.05) in chlorhexidine rinse group, plaque control group was significantly reduced at 2, 4, 6 weeks and 1, 2 and 4 weeks in control group. 7. Percentage of spirochetes was significantly reduced during all weeks (P<0.05), plaque control group was significantly reduced at 2, 4, 6 weeks and 1, 2, 4 weeks in control group. This results were suggested that clinical and microbiological effect of chlorhexidine rinse and supragingival plaque control following scaling and root planing on periodontal disease

중환자실 시설기준 작성을 위한 기본시설 수준 설정 연구 - 국내·외 시설기준 및 국내 종합병원 병상규모 별 시설 현황분석을 기초로 하여

윤형진,조준영,Yoon, Hyung Jin,Cho, Jun Young 한국의료복지시설학회 2017 의료·복지 건축 Vol.23 No.3

Purpose : This study is a basic research to establish guidelines for healthcare facility in Korean healthcare market. As a first step, it is a main purpose for making beginning point of ICU guideline to set a basic level of ICU facility size and quality by analysis current ICU facilities and existed domestic guidelines : law, criteria of healthcare insurance review and assessment service, credit of Korea institute for healthcare accreditation. Methods : First of all, the all requirements of existed guidelines are put together and summarized. The summary is compared with that of US, UK, and Australia to know its quality difference with international market. In addition, all hospitals in Korean market are classified based on total bed number and ICU bed number to know the most occupied facility size range in the market. Second of all, by comparing 15 ICUs' current setting of the public general hospital, a general condition of ICU facility would be extracted based on function, floor area per bed, services Results : 72.8% of hospitals in Korea are belonging below 500 beds hospital. Among them 200-299 beds hospitals occupied 35.3% and are shown as the most occupied hospital size. As 15 public general hospitals are analyzed, it is clear that the more bed size and services the more ICU area per bed. As a result it is sure that the 300~500 bed may be a clear line as a general ICU condition whose function and bed number relatively consistent in the range. Implications : to keep the qualified medical environment and contemporary hospital trend, the guideline as a minimum requirement keep naturally out from current healthcare settings and should reflect their limit to reconcile with the new trend in the market.

국가지정입원격리병상의 시설별 면적구성에 관한 연구 - 2016년 국가지정입원격리병상 확충사업대상을 중심으로

윤형진,권순정,Yoon, Hyung Jin,Kwon, Soon Jung 한국의료복지건축학회 2017 의료·복지 건축 Vol.22 No.3

Purpose: Since the facility guidelines for National Designated Isolation Unit wards(NDIUs) had been edited since 2016, all hospital who want to expand or install NDIU should adapt the new guidelines. Instead of providing area requirement, by the way, only essential or optional facility requirements are suggested except patient bedroom in the guidelines. So, as analyze area and area composition of the NDIUs, it could be expected that this study has a role as an area planing reference for not only NDIU but also another airborne infection isolation room. Methods: For the area analysis, 18 sample hospitals are selected among 2016 year applicants. All rooms in NDIUs are grouped as zones whether those are negative air pressurized or not and programed room or not. At the end, area of the zones are summarized and analysed a relationship between area increase and bed number by both correlation analysis and regression analysis. In addition, department usable and gross area per bed, N/G ratio, G/N ratio, and average area ratio of each zone is calculated. Results: First of all, rooms in none negative air pressurized zone of the NDIUs haven't shown a regular installation so that only those in negative air pressurized zone are targeted for the area analysis. Second of all, patient room unit(0.92) and support area(0.79), by correlation analysis, are correlated with total net area. Patient room unit(0.94) and total net area(0.79) are also shown a correlation with bed number. Department usable area($R^2=0.63$, y=36.278x + 102) and patient room unit area($R^2=0.89$, y= 27.993x - 0.8924) has a relationship with bed number by regression analysis. Average N/G is shown as 0.85 and G/N 1.36. Average area ratio of circulation, doffing area, patient room unit, and support area are 25.4%, 9.1%, 50.9%, and 14.6% in order. Implications: This study is a basic research for exploring the NDIUs guidelines to find resonable evidence to develop it for its practical use. Still, it is possibly expected that the guideline is to be developed by post occupancy evaluation in the area of where minimum requirement or facility grade needs to be defined, and by further studies with various perspectives.

응급실에서의 감염관리 표준지침의 반영에 관한 연구

윤형진(Yoon, Hyung Jin),오준걸(Oh, Joon-Gul) 한국산학기술학회 2015 한국산학기술학회논문지 Vol.16 No.11

응급실은 병원으로 입원하는 주요 경로로서, 감염환자의 1차적 내원의 가능성이 높은 곳이다. 국내에서 질병관리본부 가 2009년에 제정한 “응급실에서의 감염관리 표준지침”이 응급실 감염환자 관리 방안과 표준시설기준을 규정하고 있으나 각 병원 응급실의 감염환자 시설은 병원마다 차이가 있고 지침에 따른 일관된 감염환자 관리시설을 갖추었다고 보기 어렵다. 본 연구는 응급실에서의 감염관리 표준지침의 실효성을 알아보기 위해 법적 강제성을 갖는 “응급의료에 관한 법률”과 비교 하여, 첫째 지침이 법률 시설기준에 반영된 여부를 분석하였고, 둘째 2009년 전후에 신축 또는 개축한 응급실들을 분석대상 으로 하여 각 시설기준 항목들의 반영여부를 파악하였다. 그 결과 지침과 법률 시설기준의 상호연관성은 없었다. 분석대상 응급실들의 시설은 법적기준을 모두 충족한 반면 지침의 감염관리시설은 선별적으로 적용되어 있었고, 설치 항목들이 건축 년도에 관계없이 분석대상 응급실에 대부분 적용되어 “응급실에서의 감염관리 표준지침”의 제정과는 무관한 것으로 나타났 다. 본 연구 결과는 향후 응급실의 감염관리시설의 법적기준 체계 보완에 참고자료로 활용 될 수 있을 것이다. Korea Centers for Disease Control and Prevention(CDC) has been provided not only manual of managing infectious patient but also functional requirement of space in emergency department(ED) by distributing “Guideline for infection control in emergency department(GICED)”in 2009. To understand how much the guideline enforces its functional requirement on ED planning practice, it is compared to Emergency Medical Service Act(EMSA) a basic standard for ED planning. As a result, it is clear that those have different focal point in functional program and don’t share infection control issue. By reviewing target hospitals’ EDs opened around 2009, all ED have satisfied with the EMSA requirement but guideline. Those are selectively adapted infection control related spaces CDC guideline suggested regardless of open year so that target EDs are not to be influenced by the guideline. This research can support as a reference research when the EMSA are going to be reinforced by infection contol issue.

호흡기 감염병 예방을 위한 보건소 상시 선별진료소 활용방안 연구 - 음압 결핵 검진실을 중심으로

윤형진 ( Yoon Hyung Jin ),한수하 ( Han Su Ha ) 한국의료복지건축학회 2021 의료·복지 건축 Vol.27 No.4

Purpose: Tuberculosis(TB) care unit in public health center should be carefully considered to be re-designed as an infection safety environment for both patient and healthcare workers. So, for the enhancement, this study analyses the facility requirements for co-using the screening clinic as a TB and other respiratory disease care unit. Methods: Not only screening clinic facility guidelines from “A Study for Standard Triage Design and Construction Document” but also the guidelines of TB care and related medical facility are reviewed; KDCA, CDC, ECDC and WHO as a TB care, and FGI and NHS for facility. The facility requirements are summarized space, approach, and mechanical requirement in order. By comparing the summary and screening clinic facility guidelines, supplementations are proposed for TB care unit setting. Results: The result of this study shows that both the space program and mechanical requirement of the screening clinic and that of TB care unit are almost identical and could be share, which include direct airflow or negative air pressure in an exam room. To increase functional and economical efficiency, however, it is necessary to consider a multi-functional negative pressured room, So care process may be re-designed based on a room type; face-to-face room or glass wall inbetween. Implications: The facility guidelines for TB care unit of a public health center should be developed to build a safe environment for infection control by reflecting its medical plan and budget.

국제표준 ISO/IEC 9126, ISO/IEC 25010, ISO/IEC/IEEE 29119와 비교한 TMMi, TPI기반 테스팅 프론티어 역량 평가 모델 개선 방안

윤형진(Hyung-Jin Yoon),최진영(Jin-Young Choi) 한국정보과학회 2014 한국정보과학회 학술발표논문집 Vol.2014 No.6

병원시설기준의 정비와 미래를 위한 준비

윤형진(Yoon, Hyung Jin) 대한건축학회 2017 建築 Vol.61 No.6

소프트웨어 제품과 프로세스 관점에서 국제표준과 비교를 통한 테스팅 프론티어 역량평가 모델 개선 방안

윤형진(Hyung-jin Yoon),최진영(Jin-Young Choi) 한국정보과학회 2015 정보과학회 컴퓨팅의 실제 논문지 Vol.21 No.2

테스팅 프론티어 역량평가 모델은 10년 전 만들어진 ISO/IEC 9126의 소프트웨어 제품 품질기준과 TMMi, TPI의 프로세스 점검 기준들을 사용하다보니, Agile, TDD와 같은 새로운 프로세스와 App, Web 같은 새로운 종류의 소프트웨어 제품을 개발하는 회사들에 대한 역량평가가 어려울 수밖에 없다. 이런 문제에 대한 개선을 위하여 1) 소프트웨어 제품 품질 국제표준 ISO/IEC 9126, ISO/IEC 25010 그리고 SW 테스팅 프로세스 국제표준 ISO/IEC/IEEE 29119 part2 고찰, 2) TCAM 소개, 3) 소프트웨어 제품품질 관점에서 ISO/IEC 9126, ISO/IEC 25010와 TCAM 비교, 분석 4) 소프트웨어 테스팅 프로세스 관점에서 ISO/IEC/IEEE 29119 part2와 TCAM 비교, 분석, 5) TCAM의 개선을 위한 방안을 제안한다. The Testing Frontier Capability Assessment Model (TCAM) is based on ISO/IEC 9126, TMMi and TPI. Since ISO/IEC 9126, TMMi and TPI were made over 10 years ago, TCAM faces the problem that it can not assess and analyze the capability of small businesses that employ new software development methods or processes, for example Agile, TDD(Test Driven Development), App software, and Web Software. In this paper, a method to improve the problem is proposed. The paper is composed of the following sections: 1) ISO/IEC 9126, ISO/IEC 25010 and ISO/IEC/IEEE 29119 part 2 review 2) TCAM review 3) software product quality perspective comparison, and analysis between ISO/IEC 9126, ISO/IEC 25010 and TCAM 4) comparison, and analysis between ISO/IEC/IEEE 29119 part2 and TCAM and 5) proposal for the improvement of TCAM.

사용자 스트레스 경감을 위한 모듈러 음압격리병동 건축계획 연구 - COVID-19 감염환자 치료 경험 의료진 및 관계자 포커스 그룹 인터뷰 내용을 중심으로 -

윤형진(Yoon, Hyung Jin),최광석(Choi, Kwangseok) 대한건축학회 2024 대한건축학회논문집 Vol.40 No.3

This study conducted a literature review on user experiences in COVID-19 medical settings in advance. Additionally, it summarized requirements from focus group interviews (FGI) concerning the architectural planning elements of the Modular Airborne Infection Isolation Ward. Consequently, the primary purpose of the modular facility and user needs based on patient severity were determined. Moreover, common planning issues and solutions, including facility usage, location & scale, space composition & circulation, equipment, and stress reduction environment, were organized as construction planning elements of the modular airborne infection isolation ward. The implications suggest that designing airborne isolation wards for mildly-to-moderately infected patients requires a different planning approach compared to high-risk infected patients, addressing the mismatched hard stay environment for relatively low-severity patients, which can stress both patients and medical staff. The modular infection isolation ward, leveraging modularity characteristics, should be adaptable to accommodate such distinctiveness and diversity.