철 과잉투여가 흰쥐의 Hepatic Fibrogenesis와 Collagen 및 TGF - βI 유전자 발현에 미치는 영향

박종환(Jong-Hwan Park),이현영(Hyun Young Lee),정연희(Youn-Hee Joung),김해영(Hae-Yeong Kim),양영목(Young Mok Yang) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.2

철 이온의 과잉 투여에 의한 흰쥐의 간 섬유화로의 진행과 이에 관련된 유전자 발현 및 세포의 미세구조와 형태변화를 분석하기 위해 흰쥐에 carbonyl iron(3%)을 함유한 사료를 섭취시켜 인위적으로 철의 과잉상태를 유발하였다. 정상 식이군과 철 이온이 함유된 사료를 섭취한 군의 간 조직에서 total RNA를 추출하여 철 이온의 투여에 의한 collagen type Ⅰ, Ⅲ mRNA 및 TGF-βⅠ mRNA의 유전자 발현 수준을 정량분석하였다. 그리고 전자현미경을 이용하여 간 세포의 미세구조의 변화를 관찰하였다. 그 결과 정상 식이군에 비해 철이온을 함유한 사료를 섭취한 군에서 type Ⅰ collagen mRNA와 TGF-βⅠ mRNA의 유전자 발현 수준이 유의적으로 증가됨을 확인하였으나, type Ⅲ collagen mRNA의 유전자 발현수준은 유의적인 차이를 발견할 수 없었다. 또한 철 이온의 과잉섭취에 의해 간 조직 내의 각 세포의 변형이 일어났으며, 이로 인하여 hepatocyte의 괴사, 세포질의 변화, HSC의 증대, 세포 소기관들의 변화, ECM 성분의 변화 및 증대, 그리고 collagen섬유의 상당한 축적을 확인하였다. Iron excess is known to affect long-term iron accumulation and tissue change such as fibrosis in liver. To determine the changes of expression level of genes associated with fibrosis by short-term iron exposure, we measured liver mRNA levels by reverse transcription polymerase chain reaction (RT-PCR) in rats fed dietary carbonyl iron (3%, wt/wt) for 9 weeks. The results showed that the expression of the collagen (Ⅰ, Ⅲ) and transforming growth factor (TGF)-βⅠ mRNAs was enhanced in high-dose iron treated rat, compared to normal-dose iron treated rat. An electron microscopy study revealed that excess iron caused increase of collagen fibrils in liver. The cell shapes and compositions of hepatocytes and extracellular matrix (ECM) in liver were changed by the iron-treatment. Also, necrosised hepatocytes were broadly seen in ECM. Taken together, we suggest that iron overload affects changes of collagen and TGF-βⅠ gene expression and these changes are associated with liver fibrogenesis.

고혈압 형질에 대한 Disease model의 개발에 관한 연구

양영목,문언수 건국대학교 1996 學術誌 Vol.40 No.2

This study was undertaken to develop Disease Models for hypertension. The risk factors of hypertension reported are genetic factors, age, body size, excercise, stress, smoking and alcohol. We selected the 2,973 persons who had been taken annual routine health examination from hospital of college of medicine, Kon-kuk University. And we studied the prevalance of hypertension and the effect of sex, age, blood glucose level(BGL), cholesterol, body mass index(BMI) and Broca Index(BRI) by Disease Models for hypertension. There were significant differences in the levels of blood pressure among body mass index, Broca index, age, sex and blood glucose level by Disease Models for hypertension. The effects of variance component of BMI, age, sex, BGL, cholesterol and error for systolic blood pressure and diastolic blood pressure were 13.1% and 13.5%, 8.8% and 7.0%, 2.8% and 4.1%, 1.7% and 1.2%, 0.3% and 0.7%, and 73.3% and 73.5%, respectively. Key Words : Disease Model, hypertension, SBP, DBP, variance component

乳牛의 泌乳能力에 影響을 미치는 遺傳과 環境效果에 관한 硏究

梁榮穆,梁熙晟 건국대학교 1989 論文集 Vol.29 No.2

This study was undertaken to investigate the genetic and environmental effects for 305-day and ME 305-day milk yield, far yield and fat percent by using three different mixed model. Lactation records were collected from 1973 to 1987 on Holstein cows in 29 dairy farms of Krung-Ki, Chung-Nam, Kyung-Nam, Kyong-Buk, and Kang-Won provinces. In total there were 778 records and 35 sires. The results obtained in this study are summarized as follows. L. The overall mean of 305-day and ME305-day adjusted lactation traits were 6205.6Kg and 7227.2Kg for milk yield, 220.4Kg and 252.6Kg for fat yield, 3.67% and 3.54% for fat percent, respectively. 2. The effects of herd and sire for all traits, the effects of age at calving for 305-day milk yield, 305-day and ME305-day fat field, ME305-day fat percent under model (2), and the effect of parity for 305-day and ME305-day milk yield, 305-day fat yield under model (3) were highly significant (P< 0.01), respectively. 3. The effect of age al calving was increased over time and after 73∼84 month's age apeared declining. 4. The effect of calving season showed that cows that calved during winter and spring season produced more milk yield and fat yield than cows that calved during summer and autumn season, and that cows that calved during summer season were the highest fat percent. 5. The effect of parity was increased over time and third lactation was the highest milk yield and forth lactation was the highest fat yield, but third lactation was the lowest fat percent. 6. The sire of number 22 was the highest rank for milk and fat yield, the sire of number 32 was the highest rank for fat percent. 7. Use of mixed model(3) that parity was added to mixed model(2) caused to change the ranking of the sires. 8. The sire and error component of variance for milk yield and fat yield were increased, and the error component of variance for fat percent was decreased by age adjustment. The heritabilities for milk yield, fat yield and fat percent of 305-day and ME305-day were increased in ME305-day, were 0.25 and 0.29, 0.25 and 0.27, 0.43 and 0.58, respectively. 9. In the correlation, between age at calving and parity was high and positive value (0.93), between milk yield and fat yield was positive values (0.78∼ 0.79), between fat yield and fat percent was positive values (0.29∼0.31), between milk yield and fat percent was negative values(-0.32∼-0.36).

PHA로 자극된 T cell에서 분비된 미지의 물질이 지니는 Human Neutrophil의 수명연장, Superoxide 및 Leukotriene C₄생산증가 작용효과

허억,양영목 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-

There are several direct and indirect ways in which T cells could enhance the anti-bacterial capabilities of neutrophils. However it is not yet clear out which molecule or cytokine produced by T cells is involved in the phagocytic action and viability sustaining activity of neutrophils. The aim of this study was about a factor, which produced by the phytohaemagglutinin(PHA)-stimulated T cells, may control those neutrophil actions. Human peripheral blood T cells and neutrophils were isolated by Ficoll-paque density sedimentation from heparinized blood of healthy adult donors. The purity of these cells were more than 90%. T cells were stimulated in various dose(0.1-10gg/ml) of PHA for various times of incubation(0-3 days), and then PHA-stimulated T cell conditioned medium was collected in order to find an optimal dose and incubation time for the neutrophil viability. It was found out that 1,ug/ml of PHA in 12 hours incubation was maximal effective condition for the neutrophil sustaining viability. The effects of PHA-stimulated T cell conditioned medium(TCM) on the neutrophils were used for the comparison with PHA-nonstimulated TCM or enriched medium alone. Neutrophil sustaining viability with PHA-stimulated TCM for 24 hours incubation was significantly higher than other groups (80 ± 10 vs 25 ±15 vs 13 ± 9; p <0.01). The superoxide prodution from neutrophils with PHA-stimulated TCM for 24 hours incubation were also significantly higher than other groups(25±4 vs 11±4 vs 7±5; p <0.01). In the leukotriene C4 (LTC4) release, neutrophils with PHA-stimulated TCM for 24 hours incubation were different from other group (105 ± 20 vs 65 ±1O vs 25 ± 32 ; p <0.01), and unlikely other parameters the cells with PHA-nonstimulated TCM was different from the cells with enriched medium alone(65 -1:10 vs 25-± 32; p<0.05). In two dimension electrophoresis it was shown that PHA-stimulated T cells enhanced three proteins(66kD, 60kD, 45kD) and diminished one(40kD) in the production and/or release of proteins in comparison with PHA-nonstimulated T cells. These data suggest that these proteins from the PHA-stimulated T cells might be involved in the phagocytic actions of neutrophils.

Disease Model의 개발에 관한 기초 연구

양영목,문언수 건국대학교 의과학연구소 1994 건국의과학학술지 Vol.4 No.-

This study was undertaken to develop disease models that investigate effects of environmental factors and estimate genetic parameters for polygenic multifactorial traits, such as hypertension, diabetes, schizophrenia, allergies and cancers. The disease model (1) obtained by the linear model procedure can show significant effects on all environmental factors of the disease, and the disease model (@) can investigate effects of significant environmental factors. The genetic parameter, heritability for the disease can be estimated by regression model (6)) and genetic model (7) using the regression coefficient (b) of offspring on parent(h²=2b).

담낭담석 보유군과 비보유군 사이의 혈중 콜레스테롤 농도의 비교

이태의,양영목,문언수 건국대학교 의과학연구소 1999 건국의과학학술지 Vol.8 No.-

The aim of this study was to compare the serum cholesterol level between patients with and without gallbladder stones. Serum cholesterol levels were analyzed in 126 patients with gallbladder stones and in 99 controls. The analysis by t-test showed that patients with gallbladder stones had higher serum cholesterol level than controls in both sexes. Patients with gallbladder stones over 40 years of age had higher serum cholesterol levels than controls of same age in both sexes, but, there were no differences in serum cholesterol level between the patients with gallbladder stones and the controls below 40 years of age.

Disease Model에 의한 비만과 연령이 고혈압에 미치는 영향과 유전적 다형성에 관한 연구

문언수,양영목,박종환 건국대학교 의과학연구소 1996 건국의과학학술지 Vol.6 No.-

본 연구는 고혈압에 영향을 미치는 주요 환경요인의 하나인 비만과 연령의 효과를 Disease Model을 이용하여 측정하였으며, 앞으로 PCR(polymerase chain reaction) 기법을 이용하여 고혈압 군과 정상 혈압 군의 안지오텐신 전환효소 유전자의 intron 16에 위치한 287bp의 insertion / deletion의 유전적 다형성과 비만 및 비만 유전자, 나이 및 노화 유전자 등과의 관련성을 추적하기 위한 기초적인 자료를 얻기 위하여 실시하였다. 본 연구에서 이용된 자료는 1994년도에 실시된 직장인 성인에 대한 정기 건강진단 자료 총 2,973명에 대한 것이었다. 본 연구의 결과를 요약하면 다음과 같다. 1) 고혈압의 유병률은 수축기 혈압에서 15.5%와 이완기 혈압에서 27.2%의 높은 유병률을 나타내 이완기 고혈압 쪽에서 유병률이 더 높았다. 2) 연령에 따른 고혈압의 유병률은 남녀 모두 연령이 증가할수록 높아졌으며 40대 초까지는 남성이 높다가 40대 중반부터 남녀가 비슷하게 나타났다. 또한 남녀 모두 40대 초부터 유병률이 급격히 높아졌으며 60대 이후의 남성과 50대 중반의 여성에서는 약 60%의 높은 유병률을 보였다. 3) 비만에 따른 혈압 상승 효과는 전체 군에서는 비만도의 증가에 따라 혈압도 상승하였는데, 고혈압 군에서 SBP와 DBP 모두 BMI 값이 아주 높은 곳에서 최고 혈압치를 보였다. 4) 본 연구에서 분석된 요인들의 분산성분의 크기는 체질량지수(BMI)에서 SBP=13.1%와 DBP=13.5%로 나타났으며, 다음으로 연령에서 SBP=8.8%와 DBP=7.0%, 성별에서 SBP=2.8%와 DBP=4.1% 순이었다. 5) 고혈압에서의 유전적 다형성의 활용 등 고혈압의 원인 규명에 관련된 모든 연구 결과를 종합하고 평가하는 데 있어서 Disease Model 은 유용하게 활용될 수 있다. We studied the prevalance of hypertension and the effect of sex, age, body mass index(BMI) and Broca index(BRI(%)) by Disease Model for hypertension. We selected the 2,973 persons who had been taken annual routine health examination from hospital of college of medicine, Kon-Kuk University. Obesity is a cause of reversible hypertension that is typically associated with increases in both peripheral resistance and cardiac output. There were significant differences in the levels of blood pressure among body mass index, Broca index, age and sex by Disease Model for hypertension. The effects of variance component of BMI, age, sex and error for systolic blood pressure and diastolic blood pressure were 13.1% and 13.5%, 8.8% and 7.0%, 2.8% and 4.1%, and 75.3% and 75.4%, respectively. The Disease Models and the tools of molecular biology provide the means to define the environmental factors and the genetic basis of hypertensive diseases.

혼합 모형에 의한 젖소 종모우(種牡牛)의 육종가(育種價) 추정에 관한 연구

이광전,양희성,양영목 한국낙농학회 1988 韓國酪農學會誌 Vol.10 No.3

本 硏究에서 種牡牛의 育種價를 推定하기 위하여 使用된 資料는 1985年 11月부터 1987年 12月까지 京畿, 忠南, 慶南, 慶北, 江原 지역의 21개 牧場에서 搾乳日數 90일 이상의 618頭의 産乳 記錄을 이용하였다. 각 種牡牛의 娘牛數가 10頭 이상인 것만을 分析에 이용하였고, 그 種牡牛의 數는 24頭였다. 모든 記錄은 305日, 2回, 成牛 基準으로 補正을 하였으며, 使用된 補正係數로 USDA-DHIA의 것을 이용하였다. 分析後 얻어진 결과는 아래와 같았다. 1. GLM에 의해 補正한 乳量의 全體平均은 7531.6367㎏이었다. 2. 分析에 사용한 Model 1, 2에서 Model 1의 種牡牛의 分散은 132578.0496이고, Model 2에서의 Genetic Group이 3Group과 8Group인 경우에 150770.7385와 168732.4724였다. 3. Model 1의 Error 分散은 1878482.636이고, Model 2의 경우 1911965.352였다. 4. Model 1과 Model 2의 3Group, 8Group의 遣傳率은 0.26, 0.29, 0.32였다. 5. Model 1에서의 育種價는 -870.909㎏에서부터 +1043.93㎏에 이르기까지 다양하였고, 가장 높은 育種價를 나타낸 種牡牛는 登錄番號 1597697이었다. 6. Model 2에서의 Genetic group이 3Group인 경우에 있어서 H-Y-S의 育種價는 4492.1㎏에서 7930.54㎏까지 다양하였고, 가장 높은 育種價를 나타낸 지역은 京畿 安城이었고 Genetic group에서는 Group 3(미국)이 가장 높았으며, Genetic group 1에서 가장 높은 育種價를 나타낸 種牡牛는 登錄番號 1597697이었다. 7. Model 2에서의 3Group의 경우 全 種牡牛 중 育種價가 가장 높은 登錄番號는 1597697이었다. 8. Model 2에서의 Genetic group이 8Group인 경우에 있어서 H-Y-S의 育種價는 4313.53㎏에서 7745.51㎏까지 다양하였고, 가장 높은 지역은 3Group과 같았으며, Genetic group에서는 미국에서 1974年부터 1975年도 사이에 태어난 種牡牛 group이 가장 높았고 Genetic group 1에서 Group 8까지의 각각 가장 높은 育種價를 나타낸 種牡牛는 登錄番號 10418, 10419, 10353, 311885, 341252, 1597697, 1702698, 1705850이었다. 9. Model 2에서의 8Group의 경우 全 種牡牛 중 育種價가 가장 높은 登錄番號는 1597697이었다. This study was undertaken to estimate the sires' breeding value by mixed model procedures. Lactation records were collected from November, 1985 to December, 1987 on Holstein cows in 21 dairy farms of Kyung-Kee, Chung-Nam, Kyung-Nam, Kyung-Buk and Kang-Won provinces. In total there were 618 records sired by 24 sires, the number of daughters from each sire were more than 10. All records were standardized to 305-days, 2X milking and mature-equivalent by USDA-DHIA correction factors. The results obtained were as follows. 1. The average M.E. milk yield was estimated as 7531.6367kg by GLM procedure. 2. The sire variances were 132578.0496, 150790.7385, 168732.4724 on Model 1, 3 group of Model 2 and 8 group of Model 2, respectively. 3. The error variances were 1878482.636 and 1911965.352 on Model 1 and Model 2 respectively. 4. The heritabilities for milk yield were 0.26, 0.29 and 0.32 on Model 1, 3 group of Model 2 and 8 group of Model 2, respectively. 5. The breeding value of sires evaluated by Model 1 ranged widely from -870.909Kg to +1043.93Kg, the sire of registered number 1597697 was the highest rank. 6. In 3 group of Model 2, the breeding value of H-Y-S ranged widely from 4492.1Kg to 7930.54Kg, Kyung-Kee An-Sung region' and group 3 (U.S.A.) were the highest rank. The sires of registered number 10512, 311885 and 1597697 were the highest rank at genetic group 1, 2 and 3, respectively. 7. The sire of registered number 1597697 was the highest rank at 3 group of Model 2. 8. In 8 group of Model 2, the breeding value of H-Y-S ranged widely from 4313.53Kg to 7745.51Kg. The highest region was equal to 3 group, the sire group of U.S.A.(borned 1974-1975) was the highest rank at genetic group, the sires of registered number 10418, 10419, 10353, 311885, 341252, 1597697, 1702698, 1705850 were the highest rank of the genetic group 1-8, respectively. 9. The sire of registered number 1597697 was the highest rank of the 8 group of Model 2.

본태성 고혈압 환자에서 Angiotensin I-Converting Enzyme Gene의 유전적 다형성에 관한 연구

문언수,박종환,양영목 건국대학교 1997 學術誌 Vol.41 No.2

The ACE (angiotensin converting enzyme) is a key component of the renin-angiotensin system thought to be important in the pathogenesis of hypertension and cadiovascular disease. Deletion polymorphism in the ACE gene may be a risk factor for myocardial infarction. In the present study, ACE deletion(DD)/insertion(II) polymorphism detected by PCR analysis appears to be associated with hypertension in Koreans and its nucleotide was subcloned into T-vector and its nucleotide sequence were determined : Cloning of ACE gene revealed a 287bp Insertion/Deletion polymorphism in the intron 16. We identified the angiotensin I -converting enzyme genotype in 127 hypertensive and 189normotensive Korean subjects(total=316). There was no significant difference In genotype frequency between the hypertensive group(n=127) and the normotensive group(n=189). We found high s19nlflcant differences for ACE genotype distribution between the male group and the female group in total(P=0.001) and in hypertensive Korean subjects(P=0.013). The frequency of D allele in Korean males is higher than that of Korean females(0.451 : 0.283) in hypertensive group. Key Words : Angiotensin 1-conyerting enzyme, ACE genotype, Genetic polymorphism, Hypertension

305일 산유량 추정을 위한 보정계수(補正係數)의 개발에 관한 연구

이광전,손진기,강민식,양영목 한국낙농학회 1990 韓國酪農學會誌 Vol.12 No.3

本 硏究에서는 305日 産乳量을 推定하기 위한 補正係數를 算出하고 그 正確度를 알아보기 위해 경기, 강원, 충청, 경북지역의 38개 牧場에서 蒐集한 記錄中 1,710個의 記錄을 産次別로 2個群(1産, 2産以上) 分娩月別로 3個群(12月∼5月, 6月∼8月, 9月∼11月)으로 나누어 分析하였으며 그 結果는 다음과 같다. 1. 305日 補正係數의 算出時 모든 回歸式의 R²은 0.96∼0.99로 높게 나타났다. 2. 305日 實際産乳量과 本 硏究의 補正係數로 推定한 乳量의 相關係數는 0.96이고 平均間 有意差는 나타나지 않았다. 3. USDA 補正係數로 推定한 乳量은 實際産乳量보다 過小推定되었으며 平均間 有意差를 나타냈다(P<0.01). 4. 總乳量과 月檢定乳量과의 相關係數는 6번째 月檢定乳量이 r=0.8775로 가장 높게 나타났으며 大體로 4번째부터 6번째까지가 r=0.8689∼0.8775로 높게 나타났다. 5. 各 月檢定乳量間의 相關關係를 보면 隣接한 月檢定乳量間의 相關係數가 r=0.75∼0.84로 높게 나타났다. 6. 總乳量에 대한 累積乳量의 相關係數는 連續 5回의 月檢定乳量의 累積에서 r=0.93으로 높게 나타났고 回를 거듭할수록 1에 가까와졌다. 7. 産次別로는 2産以上이 1産보다 夏節期 分娩時 乳量의 減少가 많은 것으로 나타났다. A total of 1710 lactation records obtained from Holstein cows raised at 38 ranches in Kyong-Gi, Kang-Won, Chung-Chong, and Kyong-Puk were analyzed to calculate 305-day correction factors and to test the accuracy of the factors. Records were classified into 2 parity groups (1 and 2-7 parites) and 3 calving month groups (Dec.- May, June-Aug. and Sept.-Nov.). The results obtained in this study are summarized as follows: 1. The R² values of regression equations to calculate 305-day correction factors were high (R² = 0.96-0.99). 2. Correlation coefficient between the actual 305-day yield and the estimated yield by 305-day correction factors in this study was high (r = 0.96) and there was no significant difference. 3. The estimated 305-day milk yield by USDA factors was underestimated than the actual 305-day milk yield and there was high significant difference between the two yields (p < 0.01). 4. The correlation between total yield and monthly test yield was the highest 6th month (r = 0.88) and the R² values appeared to be the high in 4th, 5th, 6th months (r = 0.87-0.88). 5. Correlation coefficients between adjacent monthly test productions was higher than others (r = 0.75-0.84). 6. Using the cumulative monthly test records to calcualte correction factors, the cumulative productions over the 5 months were available (r = 0.93-0.99). 7. The milk yields of cows were more severely affected by summer calving than the first lactating heifers.