외식업 창업자의 특성과 서비스지향적인 노력이 창업성공에 미치는 영향에 관한 연구

신금순(Shin, Keum-Soon),양동우(Yang, Dong-Woo) 대한경영학회 2007 大韓經營學會誌 Vol.20 No.6

國民學校 兒童 第二大曰齒의 萌出 段階別 萌出時期에 關한 硏究

申金栢(Keum Back Shin) 대한치과의사협회 1982 대한치과의사협회지 Vol.20 No.11

韓國人 齒牙硬組織中 弗素濃度에 關한 硏究

申金栢(Keum Back Shin) 대한치과의사협회 1979 대한치과의사협회지 Vol.17 No.8

방사면역측정법에 의한 혈액 Cyclosporin 량 측정법

신금철 ( Keum Chul Shin ),김현주 ( Hyun Joo Kim ),서일택 ( Ii Tack Seo ) 대한임상검사과학회 1990 대한임상검사과학회지(KJCLS) Vol.22 No.1

Cyclosporin is an immunosuppressive agent. Widly used for prevention and management of graft rejection in kidney, pancreas, heart, liver and bone marrow transplantation. To optimise immunosuppressive therapy and to avoid the toxicity due to overdose following cyclosporin therapy, measurements of the drug level in whole blood or serum, plasma is indicated. Currently, two methods are in routine use for measurements of blood cyclosporin, namely high performance liquid chromatography (HPLC) and radioimmunoassay (RIA) . howerver, in aspects of routine application, the HPLC measurement of cyclosporin has some problems physiochemical properties of the molecule make its extraction, chromatography and detection relatively difficult. The RIA base on competitive binding of radiolabelled and unlabelled cyclosporin to fixed number of antibody sites is widly used in practice. In RIA, the antibody directed to cyclosporin and its metabolite are monoclonal specific or nonspecific. The data obtained from monoclonal nonspecific RIA were always 2. 5-3. 0 times higher than the HPLC results become the polyclonal antibody react crossly with cyclosporin metabolites. But the results from monoclonal specific Rf"A are comparable with HPLC method. Irrespective of the technique used to measure the cyclosporin the results is markedly affected by the choice of sample matrix. Because cyclosporin has a high affinity for erythrocyte and blood constituents, the separation protocol should be used before RIA. We now introduce the two RIA method, monoclonal specific and monoclonal nonspecific, developed from Sandoz Ltd the results of the RIA.

참나무류 목초액이 표고의 균사생장, 버섯 생산량 및 품질에 미치는 영향

신금철 ( Keum Chul Shin ),김남규 ( Nam Kyu Kim ),조종원 ( Jong Won Cho ),이상용 ( Sang Yong Lee ),이종규 ( Jong Kyu Lee ) 한국산림과학회 2008 한국산림과학회지 Vol.97 No.1

하악골 침범을 포함한 골화석증 1예

신금백,김도윤,오희명,Shin, Keum-Back,Kim, Do-Yun,Oh, Hee-Myung 대한안면통증구강내과학회 1999 Journal of Oral Medicine and Pain Vol.24 No.1

The authors experienced a diagnosis of a benign form of osteopetrosis based on the comprehensive data obtained from (1) clinical examination, (2) radiological and nuclear medicinal findings of the thickening of cortex of mandible, femur, clavicle, and the increased bone density of mandible, maxilla, cranial base, calvarium, lumbar, femur, and additionally (3) histopathological findings of sclerotic bony tissue in a Korean male of 38-year-old.

직장암 유래 전이성 구강 암종의 진단예

신금백,강기현,채규삼,Shin, Keum-back,Kang, Kee-hyun,Chae, Gyu-sam 대한안면통증구강내과학회 1999 Journal of Oral Medicine and Pain Vol.24 No.2

최근 저자들은 좌측 안검하수, 안면마비, 좌측 협궁부의 부종 및 안모비대칭을 호소하는 49세 한국 여성 환자로부터, 임상적으로 우측 하악지 부위에서의 융기성 경결성 병소를 발견하고, 이에 대한 진단 및 감별진단을 위해 (1) 문진상 직장 선암(直腸 腺癌)에 대한 과거 수술병력 소견, (2) 두부 전산화단층촬영사진상 우측 하악지 전내방에서의 비교적 균질한 괴(塊)의 존재와 이것에 의한 우측 하악지 내측 피질골의 파괴, 또한 경사대(傾斜臺)를 중심으로 뇌실질 조직에 비해 약간 고밀도를 보이는 불규칙한 괴(塊)의 존재와 이것에 의한 접형동(蝶形洞)과 주변 골 및 좌측 상악골의 파괴, 그리고 전신 골스캔상 비강 부위와 좌측 상악골 부위에서의 hot spot의 존재, 그리고 후전방 흉부 일반방사선촬영사진상 전폐야에 걸친 다양한 크기의 수 많은 결절의 산재를 나타낸 영상화 검사 소견, (3) 과거 전신병력 추적 소견상 간 우엽에서의 중심부에 hyperechoic focus를 가진 과녁 모양의 경계가 양호한 괴(塊)의 존재를 나타낸 간부위 초음파 검사 소견을 채득한 후 이들 자료를 종합, 분석, 평가한 결과, 과거 전신병력상의 원발성 직장 선암의 원격 전이에 의해 발생된 것으로 판단되었으며, 또한 영상화 검사에서 관찰된 우측 하악지 내측 피질골의 파괴상 및 좌측 상악골의 파괴상 역시 원발성 직장 선암의 원격 전이에 의해 발생된 것으로 판단되었다. The authors experienced a oral carcinoma involving the medial cortex of the right ramus of mandible and the left side of the maxilla which was metastasized from primary rectal adenocarcinoma based on the comprehensive evaluation of data obtained from (1) the past medical history of primary rectal adenocarcinoma, (2) the computerized tomographic views of the homogeneous mass on the anteromedial region of the right ramus of mandible destructing the medial cortex of the right ramus of mandible, and the irregular mass around the clivus spreading into the sphenoid sinus and destructing the left side of the maxilla ; the bone scanning of hot spots on the nasal region and the left side of maxilla ; the posteroanterior chest radiographic view of multiple nodular radiopacities on the lung, (3) the retrospective review of the sonographic view of a hypoechoic, well-demarcated, target-like mass with central hyperechoic focus on right lobe of liver in a Korean female of 49-year-old who complained the ptosis of left eyelid, the swelling on left zygomatic area, the left facial paralysis, the facial asymmetry and a round-shaped, elevated lesion with a hematoma on the mucosa covering the right ramus of mandible.

Nd:YAG레이저조사가 경화상아질에 미치는 영향에 관한 주사전자현미경적 연구

김문현,신금백,Kim, Moon-Hyeon,Shin, Keum-Back 대한안면통증구강내과학회 1999 Journal of Oral Medicine and Pain Vol.24 No.4

In order to obtain the basic data concerning the optimal parameters in using Nd:YAG laser as a therapeutic modality to dentinal hypersensitivity, the author prepared 3 sections of sound dentin and 10 sections of sclerotic dentin with thickness of $0.5mm{\pm}0.1mm$ from human extracted teeth of anteriors and premolars, and applied the laser energy from a fiberoptic delivered, free running, pulsed Nd:YAG laser (wavelength 1064nm, pulse duration $120{\mu}sec$, fiber diameter $320{\mu}m$) to surfaces of sound and sclerotic dentin sections for 1 second with contact/unidirectional moving mode of the fiber under speed of 3mm~4mm/sec and parameters of 0.5W/10Hz, 1.0W/10Hz, 1.5W/10Hz, 2.0W/10Hz: $62J/cm^2$, $124J/cm^2$, $187J/cm^2$, $249J/cm^2$. The author comparatively evaluated the characteristics of ultrastructural changes on surfaces of sound and sclerotic dentin sections irradiated by the pulsed Nd:YAG laser using the scanning electron microscopy. A fairly ill-defined bordered surface of partially closed and melted dentinal tubules can be seen on the scanning electron microscopic feature of the sound dentin surface irradiated by the pulsed Nd:YAG laser with energy density of $62J/cm^2$. The physical modification of sound dentin surface extensively occurred depended on the increase of energy density from $62J/cm^2$ to $124J/cm^2$, $187J/cm^2$, $249J/cm^2$. While, a fairly well-defined bordered surface of partially closed and melted dentinal tubules with thickened peritubular dentin can be seen on the scanning electron microscopic feature of the sclerotic dentin surface irradiated by the pulsed Nd:YAG laser with energy density of $62J/cm^2$. The physical modification of sclerotic dentin surface of a fairly rough, shallow depression with many cracks, thickened peritubular dentin and structureless dentinal tubules extensively occurred depended on the increase of energy density from $62J/cm^2$ to $124J/cm^2$, $187J/cm^2$, $249J/cm^2$ compared to those of sound dentin surface irradiated by the pulsed Nd:YAG laser under the same parameters. Therefore, it is recommended that the pulsed Nd:YAG laser as a therapeutic modality to dentinal hypersensitivity should be applied with the less energy density than $62J/cm^2$ on the sound dentin surface, and its energy density on the partially sclerotic dentin surface should be lower than that on the sound dentin surface to preserve tooth from unnecessary excessive structural destruction.

Nd:YAG 레이저조사가 치경부 백악질에 미치는 영향에 관한 주사전자현미경적 연구

최복,이정연,신금백,Choi, Bok,Lee, Jeong-Yeon,Shin, Keum-back 대한안면통증구강내과학회 2000 Journal of Oral Medicine and Pain Vol.25 No.4

In order to obtain the basic data concerning the optimal lasing conditions in using Nd:YAG laser as an adjunctive modality of periodontal therapy of root planing without irreversible structural deterioration of cervical cementum, the author selected 36 human teeth having no cervical abrasion and caries (; 12 anteriors, 12 premolars. 12 molars) extracted due to periodontal diseases, and divided them into 4 groups as Group I, II, III and IV (; each group of 3 anteriors, 3 premolars, 3 molars), and prepared a cementum specimen with thickness of $1.0mm{\pm}0.2mm$ sectioned longitudinally at the middle of mesio-distal thickness (; Group I) or horizontally at 1mm-2mm below the cemento-enamel junction (; Group II, III, IV) from each tooth of each group by low speed diamond wheel saw, and treated them with 0.5 M ethylene diamine tetraacetic acid (; EDTA, pH=7.4) for 2 minutes for elimination of remnants during tooth-sectioning. And the author applied the laser energy from a fiberoptic delivered, free running, pulsed Nd:YAG laser (; wavelength 1064nm. pulse duration $120{\mu}sec$, fiber diameter $320{{\mu}m}$) to cementum surfaces in triplicates of one experiment under the following lasing conditions: 1. stationary mode of fiber in contact to cementum surfaces without air-spray (; Group I )/with air-spray (; Group II), 2. unidirectional moving mode of fiber in contact to cementum surfaces under speed of 3mm-4mm/sec without air-spray (; Group III)/with air-spray (; Group IV), 3. energy per pulse (mJ/pulse) [; energy density ($J/cm^{2}$)] in order of 1.0W/10Hz (100J/pulse); $124J/cm^{2}$, 0.5W/10Hz (50mJ/pulse); $62J/cm^{2}$, 0.4W/10Hz (40mJ/pulse); $50J/cm^{2}$, 0.3W/10Hz (30mJ/pulse); $37J/cm^{2}$, 4. exposure time of 1 second. And the author applied the platinum coating on surfaces of cementum specimens, and evaluated the characteristics of ultrastructural change on surfaces of cementum using the scanning electron microscopy. In general the ultrastructural loss of cervical cementum irradiated under the same lasing condition of laser energy density occurred least in specimens of Group IV. And especially, the ultrastructural loss of cervical cementum irradiated under the laser energy density of $37J/cm^{2}$ almost did not occur in specimens of Group IV. Therefore, it is considered that the pulsed Nd:YAG laser should be applied with the lasing conditions of unidirectional moving mode of fiber in contact to cementum surfaces under speed of 3mm-4mm/sec with air-spray and of laser energy density within $37J/cm^{2}$ as an adjunctive modality of periodontal therapy of root planing without irreversible structural deterioration of cervical cementum.

Fusobacterium nucleatum 추출물이 사람 치은 섬유아세포와 HOS 941세포의 성장과 마우스 비장세포의 TNF-α 생성에 미치는 효과

오희명,송요한,신금백,Oh, Hee-Myung,Song, Yo-Han,Shin, Keum-Back 대한안면통증구강내과학회 1999 Journal of Oral Medicine and Pain Vol.24 No.4

F. nucleatum is a gram-negative obligate anaerobe which is the principal and most frequent cause of gingival inflammation and is the predominant pathogen isolated in subsequent periodontal breakdown. It is also one of the most numerous bacteria found in subgingival plaque samples from healthy sites; its numbers are about 10-fold greater in plaque from periodontally diseased sites. The purpose of this study is to examine the effects of outer membrane(OM), outer membrane vesicle(OMV), and lipopolysaccharide(LPS) from F. nucleatum ATCC 25586 strain on the growth of human gingival fibroblasts and HOS 941 cells, and on the $TNF-{\alpha}$ production / $TNF-{\alpha}$ mRNA expression of mouse splenocytes. For the examination of cytotoxic effects, $TNF-{\alpha}$ production and $TNF-{\alpha}$ mRNA expression, the MTT assay, the ELISA and the RT-PCR were performed, respectively. All extracts of F. nucleatum tested were cytotoxic to both of human gingival fibroblasts and HOS 941 cells, and the significant difference of cytotoxic activity among the extracts was not observed. In the effects of these extracts on the $TNF-{\alpha}$ production / $TNF-{\alpha}$ mRNA expression of mouse splenocytes, all extracts of F. nucleatum tested also stimulated the $TNF-{\alpha}$ production / $TNF-{\alpha}$ mRNA expression, but the effects of the OM extracts on the $TNF-{\alpha}$ production / $TNF-{\alpha}$ mRNA expression were higher than those of the OMV and the LPS extracts. The pattern of the $TNF-{\alpha}$ mRNA expression was similar to that of the $TNF-{\alpha}$ production. These results indicate that F. nucleatum seems to contribute to the pathogenesis of periodontal diseases at least by its cytotoxicity, directly and its $TNF-{\alpha}$ production, indirectly.