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DNA Sequencing of 120 kD Antigen of Helicobacter pylori
DOH, YOUNG MI,KIM, JAE IM,BAIK, SEUNG CHUL,CHO, MYUNG JE,LEE, WOO KON,RHEE, KWANG HO 경상대학교 유전공학연구소 1991 遺傳工學硏究所報 Vol.10 No.-
A genomic libary of Helicobacter pylori DNA in E. coli Y1090 was constructed into bacteriophage λgtll. The fusion proteins from recombinant phages were screened with monoclonal antibodies to 120 kD antigen of Helicobacter pylori. One clone(HPR4) was positive. The molecular weight of the fusion protein was 165kD. therefore, 51 kD size of the 120 kD antigen was cloned. The cloned gene in HPR4 is supposed to encode for carboxy-terminal region of 120 kD antigen. The K_pnⅠ-Sph Ⅰ fragment in 5'-terminal region of insert DNA was selected as a gene fragment corresponding to the 120 kD antigen portion(51kD) of the fusion protein. The nucleotide sequence of 1552bp was determined.
Cloning and DNA Sequencing of Flagellin Gene of Helicobacter pylori
RHEE, KWANG-HO,LEE, WON-KON,CHO, MYUNG-JE,DOH, YOUNG-MI,BAIK, SEUNG-CHUL 경상대학교 유전공학연구소 1992 遺傳工學硏究所報 Vol.11 No.-
A λgtll expression library of Helicobacter pylori DNA was screened with flagllin-specific rabbit antiserum for molecular cloning of the flagellin gene of Helicobacter pylori. A positive clone,λHPF4, was obtained and the recombinant antigen expressed from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA ahowed that an open reading frame of 1536 nucletides encodes a polypeptide with a predicted molecular size of 54kd. This open reading frame did not show any homology with flaA gene encoding 56kd protein of Helicobacter pylori amd was confirmed as a uniques sequence through homology searching. The cloned antigen is tentatively supposed to be the carboxy-termonal region of the other flagellin protein of Helicobacter pylori, flaB, with the molecular weight of 58kd.
Helicobacter pylori 편모 유전자의 클로닝 및 염기서열 분석
이광호,이우곤,조명제,도영미,백승철,강경희,박필성,이상룡 경상대학교 유전공학연구소 1993 遺傳工學硏究所報 Vol.12 No.-
A λgt11 expression libary of H. pylori DNA in E. coli Y1090 was screened with flagellin-specific rabbit antiserum for molecular cloning of the flagellin gene of H. pylori. A positive clone, λHPF4, was obtained and the recombinant antigen expressed from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA showed that an open reading frame composed of 1,536 nucleotides encodes a polypepride with a oredicted molecular size of 54kd. This open reading frame did not show the homology with flaA gene encoding 56kd protein of H. pylori and was confirmed as a unique sequence through homoligy searching. Therefore, the cloned antigen is supposed to be the carboxy-terminal region of the other flagellin protein of H. pylori, flaB, with the molecular weight of 58kd.