미역 열수 추출물의 항산화 및 아질산 제거효과

김윤수(Yoon-Soo Kim),남형근(Hyung-Gun Nam),신현재(Hyun-Jae Shin),나명순(Myung-Soon Na),김미혜(Mi-Hye Kim),이철원(Chul-Won Lee),김종수(Jong-Soo Kim),박옥란(Yu Lan Piao),차월석(Wol-Suk Cha) 한국생물공학회 2011 KSBB Journal Vol.26 No.2

For the development of functional food and cosmetics using the hot water extract of Undaria pinnatifida, the concentrations of vitamin, amino acid and element and activities of antioxidant and nitrite scavenging were investigated. The results are shown as follows: Vitamin C and E concentrations were 0.301 and 0.11 mg/100 g, respectively. Mineral concentrations were an order of Ca > Mg > K > Fe. The concentrations of total amino acids were an order of Glu > Ala > Val > Leu > Gly > Pro. Total phenol concentration and DPPH radical scavenging activity were increased with the increase of the concentration of extract. Especially, when the extract concentrationwas increased from 1.0 to 10.0 mg/mL, the total phenol concentration was increased from 0.043 to 0.125 OD 725 nm. DPPH radical scavenging activity at 50 mg/mL was 70.1%. The antioxidant activity of extract was stable in range of 80 to 140℃ and pH 3-9. The nitrite scavenging activity was increased with the decreaseof pH and the increase of it’s extract concentration. Especially, it was 83.4% at 50 mg/mL (pH 1.2). These results showed that the hot water extract of U. pinnatifida can be applied to functional food and cosmetics.

숭어 추출물을 이용한 주름개선 효과

조숙정(Suk-Jung Cho),김윤수(Yoon-Soo Kim),남형근(Hyung-Gun Nam),신현재(Hyun-Jae Shin),류은미(Eun-Mi Ryu),나명순(Myung-Soon Na),안병권(Byeong-Kwon Ahn),최두복(Du-Bok Choi),차월석(Wol-Suk Cha) 한국생물공학회 2011 KSBB Journal Vol.26 No.3

This study was to suggest the method for wrinkle improvement using Mugil cephalus extract. In order to evaluate the cosmetic product made of M. cephalus extract, the experiments were conducted with subjects and skin states for 4 weeks. Effect of cosmetics containing M. cephalus extract on the water holding capacity and evaporation of the women skin were investigated. The water holding capacity was increased after 1 h of application. But after 2 h, it was decreased. In the case of the water evaporation, it was increased with the increase of time. On the clinical trial, it was also found that water, oil, and rough level on the women skin were increased and wrinkles were improved. Side effects were also not detected during the application and treatment of cosmetics. Based on this result, the application of M. cephalus extract had positive effects on the improvement of wrinkles and skin in women. Therefore, it will be used to develop the wrinkle improvement therapy for women.

갯장어껍질 열수 추출물의 항산화 효과

신현재(Hyun-Jae Shin),김윤수(Yoon-Soo Kim),남형근(Hyung-Gun Nam),나명순(Myung-Soon Na),김미혜(Mi-Hye Kim),강형봉(Hyeong-Bong Kang),이희덕(Hee-Duck Lee),최두복(Du Bok Choi),최온유(On-You Choi),차월석(Wol-Suk Cha) 한국생물공학회 2011 KSBB Journal Vol.26 No.1

For the development of functional food and cosmetics using hot water extract of Muraenesox cinereus’s skin, contents of vitamin, amino acid and element, and antioxidant activity were investigated. The results are shown as follows: among vitamins, A(0.21mg/100 g), C (78.12mg/ 100 g), D3 (0.03 mg/100 g), E (1.97 mg/100 g) and Niacin (2.53mg/100 g) were detected, respectively.Mineral contents were an order of K > P > Na >Mg > Ca > Fe and Zn. Contents of total amino acids were an order of Pro > Gly > Arg > Glu > Phe > Ala. Especially, the sum of total amino acids was 27.17 mg/100 mL, which was about 4.0 fold higher than that of free amino acid. DPPH radical scavenging activity of hot water extract of M. cinereus’s skin at 25 mg/mL was 63.5% and did not increase at above 50 mg/mL. Activities of antioxidant enzymes in the liver of ethanol-treated rats using hot water extract of M. cinereus’s skin were investigated. Compared to control group, activities of ADH and GSH-px were decreased. In the case of CAT and SOD activity, they were increased. These results showed that the hot water extract of M. cinereus’s skin can be applied to raw macterial for functional food and cosmetics.

고주파요법과 갯장어 추출물 병행처리가 남성피부에 미치는 영향

노현주(Hyun-Ju No),김윤수(Yoon-Soo Kim),남형근(Hyung-Gun Nam),신현재(Hyun-Jae Shin),류은미(Eun-Mi Ryu),나명순(Myung-Soon Na),안병권(Byeong-Kwon Ahn),최두복(Du-Bok Choi),차월석(Wol-Suk Cha) 한국생물공학회 2011 KSBB Journal Vol.26 No.3

This study aimed to examine the treatment effect of Muraenesox cinereus extract product combined with high frequency on men’s damaged skin. The subjects were treated by a product containing M. cinereus extract combined with high frequency therapy for six weeks and tested in skin condition. First, for moisture level change, there was no significant difference between the control group treated only by high frequency therapy and the experimental group treated by a M. cinereus extract product combined with high frequency therapy. But the subjects who increased consistently in moisture level in the cheek and chin were more common in the experimental group. Second, for an oil level, prominently high increase was found in both the control group and the experimental group. Finally, for a rough level, while there was little difference in the control group between immediately after peeling and after six weeks, high improvement effect on a curve was found in the experimental group. Based on this result, cosmetics using M. cinereus extract may have a positive effect on men’s skin which is exposed to several stress factors. Accordingly, this result will contribute to developing men’s functional cosmetics.

비정제 유채유의 산패에 미치는 금속이온의 영향

김연순(Youn-soon Kim),김윤수(Youn-su Kim),남형근(Hyung-gun Nam),서광엽(Gwang-yeob Seo) 대한환경위생공학회 2009 대한환경위생공학회지 Vol.24 No.1

In order to investigate effect of metal ion and antioxidant on rancidity of crude rapeseed oil (CRO), Fe²?, Cu²?, Co²?, Ni²?, Cr²?, Sn²?, Mn²?, Zn²?, and antioxidants including BHA, Vitamin C, and Tocopherol were used. The specific gravity and refractive index of CRO were 0.92g/㎤ and 1.45,respectively. The chromaticities of light, red, and yellow in CRO were 88.6 and 98.7, respectively. Among various fatty acids, Oleic acid (C18:1) concentration was highest, 62.3% and Linoleic acid (C18:2) concentration was 19.16%. In the case of Linolenic acid (C18:3) and Palmitic acid (C16:0), they were 9.88 and 5.2%, respectively. The concentrations of unsaturated fatty acids and saturated fatty acid were 92.2 and 7.8%, respectively. The degree of expediting rancidity of CRO was an order of Fe²?> Cu²?> Cr²?> Zn²?> Ni²?> Al²?> Mn²?> Mn²?> Sn²?> Co²?> Li²?. Especially, when Cu²? and Fe²? was used, the peroxide value concentration was about 4.0 fold higher than non addition of them. The inhibition effect of rancidity of CRO using antioxidant with Cu²? and Fe²? was an order of BHA> Vitamin C>Tocopherol.

갯장어 및 버섯 혼합추출물의 아토피 및 여드름 피부개선 효과

차월석(Wol-Suk Cha),유지현(Ji-Hyun Yoo),민명자(Myung-Ja Min),남형근(Hyung-Gun Nam),김윤수(Yun-Soo Kim),신현재(Hyun-Jae Shin),김종수(Jong Soo Kim),최온유(On You Choi),김란(Ran Kim),최두복(DuBok Choi) 한국생물공학회 2010 KSBB Journal Vol.25 No.4

본 연구에서는 갯장어 및 버섯 혼합추출물을 이용하여 아토피 피부염 및 여드름의 개선효과를 확인하기 위하여 항산화 테스트와 아토피 피부염 임상시험자 15명에게 4주 동안 혼합추출물을 복용과 함께 환부에 도포하도록 하였다. DPPH 소거능은 혼합추출물 농도증가와 함께 증가하였다. 특히 혼합추출물농도가 1.0 mg/L에서 50 mg/L로 증가 할 경우 DPPH 소거능은 10.1에서 81.4%로 증가 하였다. 환원력도 추출물농도가 증가 함에 따라 증가 하였다. 특히 추출물 25 mg/L에서 75 mg/L로 증가 할 경우 0.05 OD (700 nm)에서 0.27 (700 nm)로 급격히 증가 하였고 추출물 농도가 100 mg/L일 경우는 0.31 (700 nm)였다. SOD활성은 추출물 농도가 100 mg/L일 경우는 45.7%였다. 아토피성 피부염의 가려움과 붉은 반점에 대하여 임상 시험 결과 임상시험자 모두 완치에 가깝도록 개선되는 효과를 나타내었다. 여드름 피부의 임상시험자 5명 모두 임상시험 후 염증 면포가 완화되어 여드름의 등급이 낮아지는 결과를 나타내었다. 여드름 피부의 임상시험자 모두 얼굴피부의 유수분 및 거칠기 상태가 정상피부의 수준에 이르도록 긍정적인 변화를 나타내었다. 혼합추출물을 피부에 직접 적용하여 임상시험한 결과, 아토피 피부염 및 여드름 피부의 임상시험자 모두 어떠한 부작용도 나타내지 않았다. 이상의 결과는 갯장어 및 버섯 혼합추출물이 아토피 피부염 및 여드름 피부관리를 위한 의약품 및 화장품의 원료로 개발될 충분한 가치가 있는 것으로 사료된다. This study was conducted to investigate effect of complex extract of various mushrooms and sharp toothed eel on the skin conditions with atopic dermatitis and acne symptoms. The total phenol concentration was increased in order of hot water> ethanol> ethyl acetate> petroleum ether> chloroform extract. Especially, when the hot water extract was used, it was about 2-3 fold higher than that of ethyl acetate, petroleumether, and chloroform extract. When the complex extract concentration was increased from 1.0 to 50 mg/L, the DPPH scavenging rate increased from 10.1 to 81.4%. The reduction power was sharply increased from 0.05 to 0.27 (700 nm) when the complex extract concentration was increased from 25 to 75 mg/L. However, above 100 mg/L, it was not decreased. In the case of SOD-like activity, it was 45.7% at 100 mg/L. Total numbers of patients with atopic dermatitis were 15 and 5 patients with severe acne symptoms. According to photos taken before and after the treatment and questionnaire results, considerable improvements in skin conditions are observed in the patients with atopic dermatitis and acne. For atopic patients, erythema and edema have been improved but the degree of effect was dependent on the individual’s constitution. Concerning acne, the effect of coating of the extracts was prominent for first week and the degree decreased with time till 4 weeks. The complex extracts wereefficient in soothing rash and maturation. Side effects such as a scar were not detected during the application and treatment.