Mercury Chloride가 마우스 복강대식세포 및 EMT-6 세포의 Nitric Oxide 생성에 미치는 영향

권근상,고대하,기노석,염정호,Kwon, Keun-Sang,Koh, Dai-Ha,Ki, No-Suk,Youm, Jung-Ho 대한예방의학회 1997 Journal of Preventive Medicine and Public Health Vol.30 No.2

Balb/c 마우스의 복강대식세포와 동종 마우스의 유선암에서 기원한 EMT-6 세포를 배양하는 조건에 여러 농도의 수은을 첨가하여 nitrite와 nitrite 생성의 변화를 관찰한 결과는 다음과 같다. 복강대식세포 및 EMT-6 세포가 생성하는 nitrite와 nitrate 양은 공히 배양시작 12시간 후에 생성량에 비해 24 시간 후에는 2배, 36시간 후에는 3배의 농도로 측정되었다. 이때 nitrite와 nitrate 농도 사이에 매우 밀접한 상관관계가 관찰되었다. 수음첨가에 따라 nitrite 및 nitrate 생성량은 용량 의존적 관계로 현저한 감소를 보이며, 24 시간 또는 36시간 후의 세포생존률도 역시 수은농도에 비례하여 감소되는데, 복강대식세포의 생존률 감소가 EMT-6 세포의 것에 비해 더욱 현저하였다. 이들 세포내에서 생성되는 ATP의 양은 복강세포의 경우 그 생존률과 비례하는 경향이었으나, EMT-6세포의 경우는 비교적 높은 생존률에도 불구하교 배양액내에 수은농도를 증가시킴에 따라 ATP생산은 현저히 감소하였다. 이상의 결과는 면역세포인 복강대식세포 뿐아니라 암세포인 EMT-6 세포에서도, L-arginine으로부터 nitric oxide를 생성하는 생화학적 반응이 수은에 의해 공히 억제될 수 있음을 보여준다. 한편 수은의 세포성 면역에 미치는 독성은 수은이 면역세포의 ATP생성과 관련한 에너지 대사과정의 장애을 초래하여 nitric oxide 생성에 필요한 반응에너지의 공급을 억제시키기 때문에 나타나는 현상으로 사료된다. The effects of treatment with mercury chloride on the nitrite and nitrate syntheses were observed in peritoneal macrophages from Balb/c mice and EMT-6 cells in vitro. The cells were cultured in Dulbecco's modified Eagle's medium(DMEM) with cytokines. Amounts of nitrite and nitrate in the culture media after 24 and 36 hours of culture were about 2-fold, and 3-fold of those measured after 12 hours respectively. There were very close associations Between the amounts of nitrite and nitrate measured in the culture media according to culture time. The survival rate of peritoneal macrophages was significantly decreased by mercury chloride added into the media in dose-dependent manner, however the survivals of EMT-6 cells were not influenced by mercury chloride concentration in media. Nitrite and nitrate syntheses were dose-dependently decreased by mercury chloride added in culture media. ATP synthesis also decreased in EMT-6 cells by mercury chloride. These results reported here suggest that the disorder of cell mediated immunity by mercurials could be related to the inhibition of nitric oxide synthesis which seems to be caused by the inhibition of ATP synthesis.

전방사단의 말라리아 예방약에 대한 비용-효과 분석

권근상 ( Kwon Keun Sang ),정창식 ( Jung Chang Sik ),백승곤 ( Ba다 Seung Gon ),황대환 ( Hwang Dae Hwane ),강성욱 ( Kang Sung Wook ),박순정 ( Park Soon Jung ) 국군의무사령부 2002 대한군진의학학술지 Vol.33 No.1

RAW 264.7 Cell에서 납에 의한 NO 생성의 조절에 미치는 Glutathione의 효과

오경재,권근상,윤욱희,신새론,Oh, Gyung-Jae,Kwon, Keun-Sang,Yoon, Wook-Hee,Shin, Sae-Ron 대한예방의학회 2002 Journal of Preventive Medicine and Public Health Vol.35 No.4

Balb/c 마우스의 복강내에 Abelson leukemia virus (A-MuLV)를 주입하여 발생시킨 대식세포주 RAW 264.7 세포의 배양조건에 납과 NAC및 BSO를 첨가하여 세포생존율과 NO 및 ATP 생성량의 변화를 관찰한 결과, 납을 처리한 기본배양조건에서 RAW 264.7 세포의 생존율은 각 농도에서 차이가 없었으며 NO의 생성량은 $0.5{\mu}M$의 납농도에서부터 용량 의존적으로 감소하였으나 ATP의 생성량은 각 농도군에서 차이가 없었다. NAC을 전처리하고 납을 처리한 배양조건에서의 NO 및 ATP의 생성량은 대조군과 차이가 없었다. BSO를 전처리하고 납을 처리한 배양조건에서의 NO의 생성량은 납만 처리했을 때와 달리 각각의 농도군에서 대조군과 차이가 있었다. ATP생성량은 역시 차이가 없었다. 이상의 결과에서 납의 농도가 증가함에 따라 ATP의 생성량은 변화가 없으면서 NO가 감소하는 것을 볼 때, 납에 의한 대식세포에서 NO생성의 억제기전은 수은 및 카드뮴 등과 같이 미토콘드리아에 영향을 미쳐 ATP생성이 억제됨으로 L-arginine-NO경로에서 ATP를 필요로 하는 iNOS가 작용을 못하여 NO 생성이 저하되는 기전과는 다른 기전이 있음을 보여준다. 또한 iNOS의 조효소인 세포내 GSH를 증가시키는 NAC을 전처리했을 때 NO의 생성량이 대조군 수준으로 회복되고 세포내 GSH를 감소시키는 BSO를 전처리했을 때는 오히려 NO의 생성량에 영향을 미치지 않는 납의 농도에서조차 NO 생성의 감소가 일어난 것으로 볼 때 GSH는 대식세포에서 NO생성을 저하시키는 납의 독성에 보호작용이 있음을 확인할 수 있었다. Objectives : To evaluate the elect of glutathione(GSH) on lead induced modulation of nitric oxide(NO) synthesis, and to examine how lead modulates NO production in macrophages. Methods : This study was observed in a culture of RAW 264.7 cells, which originated from a tumor in a Balb/c mouse that was induced by the Abelson murine leukemia virus. The compounds investigated were lead chloride, N-acetyl-cystein(NAC), and Buthionine Sulfoximine( BSO). Results : ATP synthesis in RAW 264.7 cells was unchanged by each lead concentration exposure in a dose dependent manner. The NO synthesis was decreased when exposed to lead($PbCl_2$) concentration $0.5{\mu}M$. The presence of $300{\mu}M$ NAC, used as a pretreatment in the culture medium, caused the recovery of the lead induced decrease in NO synthesis, but in the presence of $300{\mu}M$ BSO as a pretreatment, there was no recoverey. Pretreatment with NAC and BSO had no affect on ATP synthesis at any of the lead concentrations used. Conclusions : These results indicated that GSH has a protective effect toward lead toxicity, and suggested that the inhibition of NO production in macrophage due to lead toxicity may be related to cofactors of iNOS (inducible nitric oxide synthase)

도로교통소음에 대한 주민들의 반응

고대하,염정호,권근상,Koh, Dai-Ha,Youm, Jung-Ho,Kwon, Keun-Sang 한국환경보건학회 2004 한국환경보건학회지 Vol.30 No.3

Valid evaluation of community reaction to road-traffic noise exposure is important for the policy making and quality of life. The purpose of this study is to evaluate the relationship between community reaction and nighttime road-traffic noise. The study measured nighttime roadside noise caused by high traffic which is greater than LAeq(22:00-07:00) 65dB(A) and lower than 65dB(A) at Chonju city, from March to April, 2003. Three hundred sixty seven subjects, aged from 20 to 65, were selected from those who are residing close to the major roads. They were asked to answer the questions regarding noise source, stress(PWI-SF), annoyance, disturbance of specific activity, disturbance of sleep, somatic symptom, and four suggested confounding factors. The relationship between annoyance, somatic symptom and noise exposure was not significant. Adjusted odds ratio(95% C.I.) for disturbance of communication and disturbance of attention and rest were 1.59(1.03-2.71), 1.64(1.06-2.81), respectively. On the other hand sleep disturbance was indicated as 1.34(0.77-2.32). The results suggested that federal policy-making about road-traffic noise should consider community reaction evaluated by various perspective including annoyance, disturbance of specific activity, disturbance of sleep, and confounding factors.

소아에서 일차성 방광요관역류의 자연소실율 및 관련 인자

강은영,김민선,권근상,박은혜,이대열,Kang, Eun-Young,Kim, Min-Sun,Kwon, Keun-Sang,Park, Eun-Hye,Lee, Dae-Yeol 대한소아신장학회 2007 Childhood kidney diseases Vol.11 No.1

목적 : 소아의 일차성 방광요관역류는 요로감염과 이로 인한 신손상의 위험성이 높지만 방광이 성장함에 따라 자연 소실되거나 호전되는 경향이 있다. 본 연구에서는 일차성 방광요관역류의 임상적 특성과 자연소실율 및 그와 관련된 인자들을 분석하고자 하였다. 방법 : 1991년 10월부터 2003년 7월까지 본원 소아과에서 일차성 방광요관 역류로 진단되어 예방적 저용량 항생제 치료를 받았던 총 149명 소아를 대상으로 후향적으로 의무기록을 조사하였다. 대상 환아들은 12-18개월마다 동위원소 방광요도 조영술을 실시하여 역류가 소실되지 않은 경우 3년 이상 추적 검사하였다. 결과 : 대상 환아 149명 중 남아는 73.2%로 여아보다 많았고, 1세 미만은 69.1%로 이 중 남아가 86.4%로 대부분이었다. 역류의 진단 당시 평균 연령은 $16.1{\pm}21.1$개월이었고, 임상 양상으로는 요로 감염이 91.3%로 가장 많았다. 역류의 자연 소실 median time은 24개월이었고, 총 3년 누적소실율은 61.7%였다. 역류의 자연 소실과 관련된 인자들 중 남아, 1세 미만 영아, 낮은 역류의 등급, 일측성 역류, 산전 초음파상 수신증이 있어 역류가 진단된 경우, 형제간에 역류의 가족력이 없었던 경우, 진단당시 신 스캔상 신피질 결손이 없었던 경우, 추적 검사상 요로감염 재발 및 신손상이 없었던 경우의 자연 소실율이 더 높았다. 단변량 분석과 다변량 분석을 이용하여 역류의 자연소실과 독립적으로 관련된 유의한 인자들로는 1세 미만 영아 relative risk 1.77(P<0.05), 낮은 역류 등급(I+II) 2.98(P<0.05), 추적 중 신반흔 형성이 없는 군2.23(P<0.05), 신위축이 없는 군 5.20(P<0.01)이었다. 결론 : 본 연구결과 소아의 일차성 방광 요관 역류는 높은 자연 소실율을 보였으며, 특히 영아기에 진단된 경우 IV 등급 이상의 높은 등급에서도 자연 소실율이 높아 조기의 수술적 치료는 피하고 약물요법을 지속하는 것이 바람직하다. 또한 역류의 자연 소실은 진단 당시 연령, 역류의 등급과 추적 검사상 신반흔 및 신위축의 유무와 관련이 있을 것으로 사료된다. Purpose : To analyze the clinical characteristics, spontaneous resolution rate and predictive factors of resolution in children with primary vesicoureteral reflux(VUR). Methods : Between October 1991 and July 2003, 149 children diagnosed with primary VUR at Chonbuk National University Hospital were reviewed retrospectively. All of the patients were maintained on low-dose antibiotic prophylaxis and underwent radionuclide cystograms at 1 year intervals over 3 years after the initial diagnosis of VUR by voiding cystourethrogram was made. Results : The median time to resolution of VUR was 24 months and the total 3 year-cumulative resolution rate of VUR was 61.7%. The following variables were associated with resolution of VUR according to univariate analysis-; age<1 year, male gender, mild grade of reflux, unilateral reflux, congenital hydronephrosis as clinical presentation at time of diagnosis of VUR, absence of focal defects in the renal scan at diagnosis, absence of recurrent UTI, renal scars and small kidney during follow-up. After adjustment by Cox regression model, five variables remained as independent predictors of VUR resolution; age<1 yew, relative risk 1.77(P<0.05), VUR grade I+II 2.98(P<0.05), absence of renal scars 2.23(P<0.05), and absence of small kidney 5.20(P<0.01) during follow-up. Conclusion : In this study, spontaneous resolution rate of VUR, even high grade reflux, is high in infants during medical management, and it was related to age, reflux grade at diagnosis, absence of renal scars and small kidney during follow-up. Therefore early surgical intervention should be avoided and reserved for the selected groups.

일부 지역 주민들의 스트레스 관련요인에 대한 연구

이정미,길상선,권근상,오경재,Lee, Jeong-Mi,Kil, Sang-Sun,Kwon, Keun-Sang,Oh, Gyung-Jae 대한예방의학회 2003 예방의학회지 Vol.36 No.2

Objectives : This study evaluated the stress of community residents using the General Health Questionnaire, GHQ-60, as an instrument of stress measurement. Methods : The study included 2100 residents, aged 20 and over, living in three areas, a large city, a medium sized city and a rural area, between June and September 2001. A questionnaire interviewing method was used to collect data. The data were analyzed using a t-test, ANOVA, Pearson's correlation coefficients and multiple regression analysis. Results : In this study, the degree of stress, as measured by the GHQ-60, was shown to be significantly higher in the following categories: females, people over 60 years old, people engaged in the primary industries and labor work, low incomes, the divorced and the bereaved, people who received no more than an elementary education, people who suffer from chronic diseases and non-exercisers. A factor analysis suggested that there were three factors of social dysfunction factors; psychosomatic symptom, and depression and anxiety, The social dysfunction factors was statistically significant for the groups described above. The factor of psychosomatic symptoms was statistically significant in the rural residents, and in the groups describedabove. The depression and anxiety factor was statistically significant in the large city residents, people aged between 20-29 years, students, unmarried persons, university graduates and those having suffered from chronic diseases. From the multiple linear regression analyses, chronic disease, exercise, gender and income, proved to be significant stress related factors Conclusions : This study suggests that special attention should be given to the management of the chronic invalided, non-exercisers, females and snail income earners, in order to maintain and promote the psychological health of residents in a community.

Mercuric Chloride에 의한 MDCK 세포의 세포사멸

이주형,염정호,권근상,Lee, Ju-Hyoung,Youm, Jung-Ho,Kwon, Keun-Sang 대한예방의학회 2006 예방의학회지 Vol.39 No.3

Objectives: Mercury is a hazardous organ-specific environmental contaminant. It exists in a wide variety of physical and chemical states, each of which has unique characteristics for the target organ specificity. Exposure to mercury vapor and to organic mercury compounds specifically affects the CNS, while the kidney is the target organ for inorganic Hg compounds. Methods: In this study, mercury chloride $(HgCl_2)$ was studied in a renal derived cell system, i.e., the tubular epithelial Madin-Darby canine kidney (MDCK) cell line, which has specific sensitivity to the toxic effect of mercury. MDCK cells were cultured for 6-24 hr in vitro in various concentrations (0.1-100 M) of $HgCl_2$, and the markers of apoptosis or cell death were assayed, including DNA fragmentation, caspase-3 activity andwestern blotting of cytochrome c. The influence of the metal on cell proliferation and viability were evaluated by the conventional MTT test. Results: The cell viability was decreased in a time and concentration dependent fashion: decreases were noted at 6, 12 and 24 hr after $HgCl_2$, exposure. The increases of DNA fragmentation were also observed in the concentrations from 0.1 to 10 M of $HgCl_2$ at 6 hr after exposure. However, we could not observe DNA fragmentation in the concentrations more than 25 M because the cells rapidly proceeded to necrotic cell death. The activation of caspase-3 was also observed at 6 hr exposure in the $HgCl_2$ concentrations from 0.1 to 10 M. The release of cytochrome c from the mitochondria into the cytosol, which is an initiator of the activation of the caspase cascade, was also observed in the $HgCl_2-treated$ MDCK cells. Conclusions: These results suggest that the activation of caspase-3 was involved in $HgCl_2-induced$ apoptosis. The release of cytochrome c from the mitochondria into the cytosol was also observed in the $HgCl_2-treated$ MDCK cells. These findings indicate that in MDCK cells, $HgCl_2$ is a potent inducer of apoptosis via cytochrome c release from the mitochondria.

NO 및 H₂O₂가 니켈의 세포독성에 미치는 영향

오경재(Gyung-Jae Oh),권근상(Keun-Sang Kwon) 대한직업환경의학회 2003 대한직업환경의학회지 Vol.15 No.1

한 대학병원 간호사의 직무스트레스와 사회심리적 스트레스와의 관련성

김환철(Hwan-Cheol Kim),권근상(Keun-Sang Kwon),고대하(Dai-Ha Koh),임종한(Jong-Han Leem),박신구(Sin-Goo Park),신주연(Joo-Youn Shin),이의철(Yeui-Cheol Lee),김용규(Yong-Kyu Kim) 대한직업환경의학회 2006 대한직업환경의학회지 Vol.18 No.1

보건진료원 업무활동의 시대에 따른 비교

염정호(Jung-Ho Youm),권근상(Keun-Sang Kwon) 한국농촌의학 지역보건학회 2004 농촌의학·지역보건 Vol.29 No.1