한우 송아지의 질병발생과 폐사율 조사

강문일 ( Mun Il Kang ),한동운 ( Dong Un Han ),정용운 ( Yong Un Chung ),정도영 ( Do Young Chung ),이채용 ( Chai Yong Lee ),이정길 ( Chung Gil Lee ),위성환 ( Sung Hwan Wee ),조재진 ( Jae Jin Cho ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.3

From September 1996 to September 1999, 419 Korean-native calves with diseases under 6-month old collected from Kyonggi, Chungcheong, Chonlla and Kyongsang were examined by clinical, microbiological, parasitic, hematologic and histopathological mean. Among them, 124 cases were tested about the neutralization antibodies against infectious bovine rhinotracheitis virus(IBRV), parainfluenza-3 virus(PI-3V), bovine viral diarrhea virus(BVDV), bovine ephemeral fever virus(BEFV). In calf diseases in the survey, enteric diseases(72.8%) were most frequently involved and the following orders were taken by respiratory(17.4%) and reproductive(5.0%) disorders. In the causative pathogens associated with calf diseases and motality, 48.4% was induced by bacteria origin and also 35.6% by viral agents. Calf mortality was up to 76.3% in the cae of bacterial diseases and 55.7% in viral diseases. In bacterial diseases, frequent disorders were composed of colibacillosis(52.7%), salmonellosis(13.8%), pasteurellosis(12.8%) and campylobacteriosis(3.9%) and their mortalities showed 73.8% in colibacillosis, 73.0% in pasteurellosis, 67.9% in salmonellosis and 50.0% in campylobacteriosis(50.0%). Among the outbreaks of viral diseases, there were BVD(22.8%), bovine rotavirus infection (20.8%), bovine coronavirus infection(16.8%), bovine respiratory syncytial virus infection(15.4%), IBR(15.4%), Akabane disease(4.7%) and Chuzan diseases(3.4%). Interesting results through this studies were obtained the first isolate to Chuzan virus and Ainovirus in Korea which could be promised the development for diagnostic method and vaccines soon. Calf mortality to Akabane and Chuzan diseases was 100%. Main parasitic diseases were occupied by coccidiosis and babesiosis and their mortality of babesiosis was 20.0%. Other diseases were abomasal impaction(6.7%) and toxicosis(4.5%). The mortality of abomasal impaction was 89.3%. In some causes with malformations(1.9%) were confirmed as anasarca, derodidymus, polymelia, humerus hypoplasia, and tracheal collapse. Calf diseases had mostly been occurred in one month old group(52.5%) and its prevalence was 25.1% in two to three month old group and 22.4% in four to six month old group. In calf mortality by age, there were 37.9% in one month old group, 18.1% in two and three month old group, and 13.8% in four to six month old group, respectively. The older the age of calf, the less the prevalence of calf enteric diseases. Respiratory diseases in calves to be tested frequently occurred in one to two month old group (41.4%). In one month old calves, the prevalence of enteric disease was 80.0%(p<0.05) and that of reproductive and respiratory disease was 9.5% and 8.2%, respectively. In two month old and four to six month old, enteric disease was 65.7% and 63.8% and respiratory disease was 28.6% and 26.6%. Seasonal prevalence and mortality of Korean-native calf diseases were not a significant difference. Prevalence of calf diseases in summer(31.5%) frequently occurred to compare that in winter(20.3%). Abortion and malformation in calves frequently occurred in spring. Hematological values in 84 calves with clinical signs showed mild to marked leukocytosis. Also, there was slight increase in hematocrit, platelet, mean corpuscular volume and mean plasma volume, but all of those were included the higher level to normal ranges. Calves with respiratory signs showed slightly erythrocytosis. One hundred seventy three calves without clinical signs were not significant different to ill cases in hematological values, but number of platelets was in higher normal range. In 125 calves, 84.8% was taken the antibody to IBRV, but 72% with the antibody had recorded the titer level lower than log(2)5. The neutralizing antibody levels of higher than log(2)5 to PI-3V and BVD virus were 60.8% and 67.2% cases, respectively. There were the cases of 57.6% had the neutralizing antibody level lower than log(2)5 to BEFV.

국내 pasteurella anatipestifer 분리주의 오리에 대한 실험병리힉적 관찰

강문일(Mun Il Kang),고홍범(Hong Bum Koh) 한국예방수의학회 1993 예방수의학회지 Vol.17 No.1

The purpose of the study was pathologically to understand the pathogenecity of ducklings to a Korea isolate of Pasteurella anatipestifer (Pa) according to previous vaccination or not. One hundred eight ducklings were equally divided by six groups including 5 groups challenged with Pa after the inoculation of each killed vaccine prepared by undiluted (6×10⁹CFU/ml), ×5-, ×10-, ×20-, ×40- diluted concentrations of Pa, respectively, and one control group only inoculated with Pa without previous vaccination. Each bacterin was subcutaneously inoculated 2 times at 2 and 3-week old, and at 1 week after the final vaccination, all ducklings were subcutaneously inoculated with 0.5ml of Pa (6×10⁹CFU/ml) per head and sacrificed at 2 weeks later. Clinical signs were examined during the duration between the last vaccination and just before necropsy and all including dead and live cases during the experiment were carried out gross and histopathological examination. l. Among groups, the prevalence of clinical signs and mortality was gradually increased toward the groups inoculated with bacterins which made higher Pa-diluted concentration before challenge with Pa. Group treated with bacterin made by Pa-undiluted concentraion was no clinical signs and mortality, On the contrary, other groups with vaccination showed from 27.8% to 77.8% in clinical signs and from 11.1% to 27.8% in mortality. Control group was 100% clinical signs and 72.2% mortality. 2. Main clinical signs were early ocular discharge, greenish diarrhoea, sharp decrease of weight gain, ataxia, recumbency, torticollis, tremor of neck and death. 3. Principal gross findings were consisted of epicarditis and myocarditis, endocardial hemorrhage, increased volume of epicardial fluid with yellowish to red in colour, fibrinous airsacculitis and perihepatitis, mild splenohepatomegaly, pulmonary edema and congestion, petechiation of tracheal mucosa, and meningeal congestion and hemorrhage. 4. Main histopathological lesions comprised epicardium covered with fibrinous exudate including lots of heterophils, macrophages and homogenous eosinophilic fibrinous material, myocardium with edema, mononuclear cell infiltration and sometimes calcification in heart, diffuse vacuolar degeneration of hepatocytes and mononuclear cell infiltration in varing degrees of periportal areas in liver, mild hyperplasia of lymphocyte and reticular cells of spleen in live cases at autopy, but mild to marked deposition of eosinophilic substance and lymphocyte depletion in white pulp and compression of red pulp of spleen in dead case, mild to marked meningitis with many lymphocytes and few heterophils, perivascular lymphocytic infiltraion in brain and spinal cord, diffuse edema and congestion of interlobular space and lymphocytic hyperplasia around parabronchus of lungs. 5. Generally, the group treated with bacterin prepared by Pa-undiluted concentration prior to challenge showed milder severity and degree of pathological lesions than those in the other groups with vaccination. Grossly, the former was recorded just as 5.6% in their prevalence, but the latter was ranged from 50.0% to 88.9%.

돼지의 위궤양(胃潰瘍)에 관한 병리학적(病理學的) 연구(硏究)

강문일,임봉호,이정길,Kang, Mun-Il,Rim, Bong-Ho,Lee, Chung-Gil 대한수의학회 1982 大韓獸醫學會誌 Vol.22 No.1

Stomachs from 3,354 fattening pigs were examined at slaughter during the period from September 1980 to August 1981. Pigs of both sexes and $Landrace{\times}Hampshire$ crossbreds were included in the present studies, and they weighed about 60-120kg. Gross pathologic alterations of the stomach were classified as normal, epitnelial change, erosion, ulcer and scar formation. Representative tissue sections were taken from the stomach lesions at random and fixed in 10% buffered formalin. Cut sections were stained with hematoxylin and eosin, and examined histopathologically. The results obtained in the present studies were as follows. 1. In the seasonal prevalence of gastric ulceration, severe ulceration with erosion was shown during the Autumn and Winter, whereas mild ulceration was mainly shown during the Spring and Summer. 2. Of the 3,354 stomachs of the pigs, 20.8% were found to be normal. Of the rest, 40% had epithelial changes, followed by erosion (24.7%), ulcer (13.6%) and scar formation (0.9%), respectively. 3. In the prevalence of ulcers in the different regions of the stomach, the fundic region had the highest rate (61.6%) of ulceration, followed by cardiac (21.7%), esophageal (15.0%) and pyloric region (1.7%). 4. The principal gross changes were severe epithelial changes with keratotic proliferation in the esophageal region, and in the fundic region severe folding of the stomach wall was covered with bloody mucous exudates. 5. Main histopathological changes were inflammatory cell infiltrations in most cases, hemorrhages in acute ulcers and prominent proliferation of granulation tissues in chronic ulcers.

돼지의 Anisakis형충증(型蟲症)에 관(關)한 병리학적(病理學的) 연구(硏究)

강문일,임봉호,이정길,Kang, Mun Il,Rim, Bong Ho,Lee, Chung Gil 대한수의학회 1981 大韓獸醫學會誌 Vol.21 No.1

During the studies on the esophagogastric ulcers in swine, Anisakis type larvae were found associated with stomach lesions. 1. Of the 1,531 pigs examined, 11(0.7%) were infested with the parasite in the stomach. 2. The parasite was identified as Anisakis type I larvae, according to the morphological characteristics. 3. On gross examination, the parasites were seen penetrating gastric mucosa, resulting in hemorrhagic ulcers. 4. Main histopathological features were edema, massive eosinophilic infiltrations around the parasite, necrosis and perivascular eosinophilic accumulations in the submucosa.

형태학적 영향에 따른 수소 생산용 SPE - electrocatalysts 의 특성 연구

강문식,한학수,설용건,조영일 ( Mun Sik Kang,Hak Soo Han,Young Gun Shul,Yung Il Joe ) 한국화학공학회 1996 화학공학의이론과응용 Vol.2 No.2

물 전기분해용 SPE-composites의 제조 및 특성 고찰

강문식(Mun Sik Kang),오정훈(Jeong Hun Oh),심규성(Kyu Sung Sim),한학수(Hak Soo Han),설용건(Young Gun Shul),조영일(Yung Il Joe) 한국수소및신에너지학회 1995 한국수소 및 신에너지학회논문집 Vol.6 No.2

SPE-composites which was prepared with impregnation-reduction method for hydroen production were investigated with morphological and electrochemical analysis. As Pt reagent`s concentration increased, Pt loadings increased. However, reductant`s concentration had a little effect on Pt loadings. By the morphological analysis, it was found that Pt was deposited inside of SPE. Furthermore, with electrochemical analysis, we found that Pt loading, electricity, roughness factor and active surface area were 2.05 mg/cm², 14.20 mC, 21.55, 10.51 cm²/mg respectively at reducing agent concentration 0.05 ?/L. Therefore, we found the deposited Pt inside of SPE would give an effect on electrical characteristics of SPE-composites.

단크론항체를 이용한 광견병바이러스의 면역병리조직학적 진단

강문일,박남용,송재영,Kang, Mun-il,Park, Nam-yong,Song, Jae-yeong 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.2

For a immunohistochemical diagnosis of the frozen and paraffin-embedded tissues against rabies virus, mice were intracerebrally inoculated with challege virus standard(CVS) rabies virus and then were used to detect the rabies viral antigen by the immunoperoxidase(IP) and the avidin-biotin complex(ABC) method. In this study, the results confirmed that ABC and IP methods, although the former showed more specific and sensitive than the latter, were reliable and effective for the demonstration of rabies virus in both frozen and paraffin-embedded brain tissues prepared from rabies-infected mice. Additionally, IP technique using the monoclonal antibody against rabies virus could be recommended as a standard diagnostic tool instead of the present immunofluorescent method for the local veterinary services in Korea.

국내 사육 돈군내 Porcine cytomegalovirus에 관한 혈청역학적 연구

강문일,한미,한동운,김희선,김병한,김홍집,안수환,Kang, Mun-il,Han, Mi,Tajima, Tomoko,Han, Dong-un,Kim, Hee-sun,Kim, Byung-han,Kim, Hong-jib,Ahn, Soo-hwan 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4

The purpose of this study was to investigate the confirmation and prevalence of porcine cytomegalovirus (PCMV) infection of pigs in Korea using enzyme-linked immunosorbent assay (ELISA). Four hundred-eighty one sera tested were collected from the areas of Kyonggi, Kangwon, Chungcheong, Cholla, Gyongsang and Cheju during the year of 1991 to 1997 except 1994. PCMV antigen, OF-1 strain, for ELISA, was prepared 19-PFT-F cell line originated from porcine fallopian tube. Positive control was used by sera made from the specific pathogen free piglets which were infected with OF-1 strain. Three hundred-sixty seven of 481 sera (76.3%) were positive against PCMV. Antibody titers of these seropositives were classified by 129 (26.8%) cases in more than 1 : 12,800, 77 (16.0%) in 1 : 6,400, 76 (15.8%) in 1 : 3,200, 44 (9.2%) in 1 : 1,600, and 41 (8.5%) in 1 : 800, respectively. Also, the seropositive pigs were divided by 87.4% (76/87) in older than 6 month-old, 73.9% (238/322) in 2~6-month old, and 73.6% (53/72) in less than 2-month old, respectively. Regional prevalence rate of PCMV infection in Korea showed 89.7% (70/78) in Chungchong, 79.8/% (83/104) in Cholla, 79.4% (143/180) in Kyonggi, 79.3% (42/53) in Gyongsang, 50% (15/30) in Kangwon, and 38.9% (14/36) in Cheju area, respectively. In the sera collected in 1991, seropositive rate was appeared as 90.2% (37/41). From 1992 to 1997 except 1994, the average infection rate to PCMV was 77.5%. Consequently, these results confirmed that PCMV in Korean piggeries was introduced at least before the year of 1991. More importantly, PCMV infection has been prevailing nation-wide in pig herds in Korea.

다이캐스팅으로 제조한 AXE710 Mg 합금의 미세조직 및 크리프 특성

강문구 ( Mun Gu Kang ),소태일 ( Tae Il So ),정화철 ( Hwa Chul Jung ),신광선 ( Kwang Seon Shin ) 대한금속재료학회(구 대한금속학회) 2011 대한금속·재료학회지 Vol.49 No.9

To develop creep resistant die-cast Mg alloys, various alloying elements, including Ca, Ce, and Sr, were added to a Mg-Al alloy. The AXE710 alloy was produced on a 320 ton high-pressure die casting machine. The microstructure and creep properties of the alloy were examined. The creep behavior was investigated at 150℃ for stresses ranging from 50 to 100 MPa. The stress exponent was derived from the relationship between normalized secondary creep rates and compensated effective stresses. It was found to be 4.9, indicating that the dislocation climb is a dominant creep mechanism.

수소 생산을 위한 SPE - composites 의 제조법에 따른 형태학적 및 전기화학적 특성 고찰

강문식,박종호,심규성,한학수,설용건,조영일 ( Mun Sik Kang,Jong Ho Park,Kyu Sung Sim,Hak Soo Han,Young Gun Shul,Yung Il Joe ) 한국화학공학회 1996 화학공학의이론과응용 Vol.2 No.1