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Considerations for insect quarantine facilities to assess the risk of industrial insects
Seungphil Chun,Taeho Ro,Haechul Park,Myoungchul Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
Precautionary measures are cost-effective for the introduction and management of industrial insects. However, the Influx of exotic pests has not been managed very well in Korea, because of the system problems such as the lack of quarantine facilities. Additionally, there is no managing facilities or regulations for their installation according to the categories about industrial insects in Korea. Firstly, we studied the foreign guidelines for arthropod containment system such as ACLs(Arthropod Containment Levels) and the applicable guidelines for insect quarantine facilities in Korea. Secondly, we classified the types of industrial insects by their characteristics and considered the practicable guidelines for installation of quarantine facilities according to the industrial insect types. Lastly, with the related references including the details about installation and management for LMO(Living genetically Modified Organism) research facilities, we discussed appropriate guidelines for installation and management of pest quarantine system in Korea.
박태호(Taeho Park),전범석(Bumseok Chun),장서일(Seo Il Chang) 한국소음진동공학회 2014 한국소음진동공학회 학술대회논문집 Vol.2014 No.10
The combined method utilizing airborne LiDAR and GIS data is suggested to extract 3-dimensional hybrid city model including roads and buildings. Combining the two types of data is more efficient to estimate the elevations of various types of roads and buildings than using either LiDAR or GIS data only. This method is particularly useful to model the overlapped roads around the so called spaghetti junction. The preliminary model is constructed from the LiDAR data, which can give wrong information around the overlapped parts. And then, the erratic vertex points are detected by imposing maximum vertical grade allowable on the elevated roads. For the purpose of efficiency, the erratic vertex points are corrected through linear interpolation method. To avoid the erratic treatment of the LiDAR data on the facades of buildings 2 meter inner-buffer zone is proposed to efficiently estimate the height of a building. It is validated by the mean value (=5.1%) of differences between estimated elevations on 2 m inner buffer zone and randomly observed building elevations.
이동 통신 단말기용 외장형 폴디드 모노폴 안테나 및 내장형 인테나
손태호(Taeho Son),정영훈(YoungHoon Joung),전홍표(Hongpyo Chun) 한국정보기술학회 2004 한국정보기술학회논문지 Vol.2 No.1
In this paper, folded monopole antenna and intenna for the Korea pes handset are designed and fabricated. The handset antenna for mobile system should be both small size and good performances. The stubby antenna based on helical structure and the retractable antenna formed a quarter wavelength antenna with a helical are commonly applied in mobile phone handset. We, in this study, design folded type monopole antenna and intenna which have better performances than conventional stubby antenna. For compensation of reducing inductance by the short length, the scheme of folding wire to increase series capacitance is used. Higher gain than helical stubby can get by widened effective areas and length of antenna using folding structure. It is shown that these new type antennas have better gain and radiation patterns than helical stubby from the measurements on 1.75~1.87 ㎓ frequency ranges.
Kong, TaeHo,Choi, Jung-Kyoung,Park, Hyeonseon,Choi, Byung Hyune,Snyder, Brian Jeffrey,Bukhari, Shefqat,Kim, Na-Kyeong,Huang, Xian,Park, So Ra,Park, Hyung Chun,Ha, Yoon Journal of Neurosurgery Publishing Group 2009 Journal of Neurosurgery Vol.111 No.1
<B>Object</B><P>Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent hematopoietic growth factor that both enhances the survival and drives the differentiation and proliferation of myeloid lineage cells. Recent studies have suggested that GM-CSF has a neuroprotective effect against CNS injury. In this paper, the authors investigated the neuroprotective effect of GM-CSF on neuron survival and locomotor behavior in a rat model of focal cerebral ischemic injury.</P><B>Materials</B><P>To understand its neuroprotective effect in vitro, GM-CSF was administered to a glutamate-induced excitotoxicity neuronal injury cell culture model that mimics the pathophysiology of focal hypoxic cerebral injury. In the animal study, the authors prepared a rat focal cerebral ischemia model by occluding the unilateral middle cerebral artery. They then examined the effects of GM-CSF administration on changes in infarct volume, apoptosis-related gene expression, and improvement in locomotor behavior.</P><B>Results</B><P>Treatment with GM-CSF significantly increased cell viability in a cell culture model of glutamate-induced neuronal injury. Furthermore, in vivo administration of GM-CSF at 60 μg/kg body weight daily for 5 consecutive days beginning immediately after injury decreased infarction volume, altered the expression of several apoptosis-related genes (<I>Bcl-2, Bax, caspase 3,</I> and <I>p53</I>), and improved locomotor behavior in the focal cerebral ischemia model.</P><B>Conclusions</B><P>The GM-CSF had neuroprotective effects in in vitro and in vivo experiments and resulted in decreased infarction volume and improved locomotor behavior. Although the specific mechanism involved in stroke recovery was not fully elucidated as it was not the primary focus of this study, administration of GM-CSF appeared to decrease the extent of neuronal apoptosis by modulating the expression of several apoptosis-related genes such as <I>Bcl-2, Bax, caspase 3, and p53</I>. Further investigations are necessary to better understand the role of GM-CSF on neural regeneration during the recovery phase of a stroke, as well as the intracellular signal transduction pathways that mediate neuroprotection.</P>
AHN, Taeho,BAE, Chun-Sik,YUN, Chul-Ho The Japanese Society of Veterinary Science 2016 The Journal of veterinary medical science Vol.78 No.4
<P>Previously, it has been suggested that the phenotypic level of albumin in peripheral blood mononuclear cells (PBMC) decreased in streptozotocin (STZ)-induced diabetic rats. Concomitantly, the production of oxidative stresses was also elevated in the diabetic PBMC compared to that of normal control. These results suggest the close relationship between PBMC-albumin and its antioxidant roles. Here, we expanded the previous studies and investigated the effect of selenium supplementation as inorganic (sodium selenate) forms on the levels of albumin expression and oxidative stress in PBMC of STZ-induced diabetic mice. Selenium intake recovered the decreased albumin levels to those of normal mice and reduced the production of reactive oxygen species (ROS). These results support that selenium intake may alleviate the etiology and pathology of PBMC in type 1 diabetic mice by restoring the decrease in albumin contents and the production of ROS.</P>
Transformation of <i>Escherichia coli</i> and protein expression using lipoplex mimicry
Yun, Chul-Ho,Bae, Chun-Sik,Ahn, Taeho Elsevier 2016 Protein expression and purification Vol.127 No.-
<P><B>Abstract</B></P> <P>We investigated a “one-step” method for transformation of and protein expression in <I>Escherichia coli</I> (<I>E. coli</I>) using a complex of <I>n</I>-stearylamine, a cationic lipid, and plasmid DNA, which mimics lipoplex-based approaches. When <I>E. coli</I> cells were treated with the cationic lipid-plasmid complex, the transformation efficiencies were in the range of approximately 2–3 × 10<SUP>6</SUP> colony-forming units. Further increase in the efficiency was obtained by co-treatment with calcium chloride (or rubidium chloride) and the complexes. Moreover, after DNA transfer, <I>E. coli</I> cells successfully expressed plasmid-encoded proteins such as cytochrome P450s and glutathione-<I>S</I>-transferase without overnight incubation of the cells to form colonies, an indispensable step in other bacterial transformation methods. In this study, we provide a simple method for <I>E. coli</I> transformation, which does not require the preparation of competent cells. The present method also shortens the overall procedures for transformation and gene expression in <I>E. coli</I> by omitting the colony-forming step.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Transformation of <I>E. coli</I> and protein expression were performed using lipoplex mimicry. </LI> <LI> Plasmid DNA was transferred to <I>E. coli</I> cells by the lipoplex. </LI> <LI> The plasmid-encoded genes were expressed in <I>E. coli</I> cells after the transformation. </LI> <LI> The protein expression was possible without preparation of competent cells or colony formation. </LI> </UL> </P>