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Park, Sook-Jahr,Park, Kyung-Ah,Park, Cjerl-Woo,Park, Won-Seck,Kim, Jeong-Ok,Ha, Yeong-Lae The Korean Society of Food Science and Nutrition 1996 Preventive Nutrition and Food Science Vol.1 No.2
Molecular weight and partial amino acid sequence of the cis, 9-cis, 12-octadecadienoate isomerase(linoleate isomerase) of Butyrivibrio fibrisovens A-38 were determined. Linoleate isomerase was isolated from the bac-teria cultured anaerobically and purified by ultracentrifugation in conjunction with Sepharose 6B column chro-matography, Phenyl sepharose 4B column chromatography and fast performance liquid chromatography (EPLC). The isomerase was single polypeptide with 19KD of molecular weight, when determined by SDS-PAGE. Fourteen amino acids sequence of N-terminal of the linoleate isomerase was N-GEIDKYPRIIKQQ determined by Edman method.
Sook-Jahr Park,Kyung-Ah Park,Cherl-Woo Park,Won-Seck Park,Jeong-Ok Kim,Yeong-Lae Ha 한국식품영양과학회 1996 Preventive Nutrition and Food Science Vol.1 No.2
Molecular weight and partial amino acid sequence of the cis,9-cis,12-octadecadienoate isomerase(linoleate isomerase) of Butyrivibrio fibrisolvens A-38 were determined. Linoleate isomerase was isolated from the bacteria cultured anaerobically and purified by ultracentrifugation in conjunction with Sepharose 6B column chromatography, Phenyl sepharose 4B column chromatography and fast performance liquid chromatography(FPLC). The isomerase was a single polypeptide with 19KD of molecular weight, when determined by SDS-PAGE. Fourteen amino acids sequence of N-terminal of the linoleate isomerase was N-GEIDKYPRRIIKQQ determined by Edman method.
Park, Seong Kyu,Park, Sook Jahr,Park, Sang Mi,Cho, Il Je,Park, Chan Ik,Kim, Young Woo,Kim, Sang Chan Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P><I>Laminaria japonica</I> has been frequently used as food supplements in many of the Asian countries and as a drug in traditional oriental medicine. This research investigated the effects of <I>Laminaria japonica</I> extract (LJE) on acute phase inflammation in a carrageenan-induced paw edema model, as assessed by histomorphometric and immunohistochemical analyses. The effect of LJE was also evaluated in Raw264.7 cells stimulated with lipopolysaccharide (LPS) in the aspect of the inhibition of nitric oxide (NO), prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>), and proinflammatory cytokines production. NO, PGE<SUB>2</SUB>, tumor necrosis factor (TNF)-<I><I>α</I></I>, interleukin-1<I><I>β</I></I>, and interleukin-6 contents were assayed by ELISA, and inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 expressions were determined by western blot analyses. In rats, LJE treatment inhibited carrageenan-induced paw edema formation and infiltration of inflammatory cells in H&E staining. LJE treatment prevented the ability of LPS to increase the levels of iNOS and COX-2 protein in a concentration-dependent manner. Consistently, LJE suppressed the production of TNF-<I><I>α</I></I>, interleukin-1<I><I>β</I></I>, and interleukin-6. Treatment of the cells with LJE caused inhibition of inhibitor of <I><I>κ</I></I>B<I><I>α</I></I> phosphorylation induced by LPS, suggesting LJE repression of nuclear factor-<I><I>κ</I></I>B activity by LPS. In conclusion, this study shown here may be of help to understand the action mechanism of LJE and the anti-inflammatory use of <I>L. japonica</I>.</P>
Protective effects of Korean red ginseng extract on cadmium-induced hepatic toxicity in rats
Sook Jahr Park,Jong Rok Lee,Mi Jeong Jo,Sang Mi Park,Sae Kwang Ku,Sang Chan Kim 고려인삼학회 2013 Journal of Ginseng Research Vol.37 No.1
Korean red ginseng is known to regulate the immune system and help the body struggle infection and disease. Cadmium is widely distributed in the environment due to its use in industry. Exposure to cadmium is problematic causing organ dysfunction. This study was conducted to evaluate the protective effect of Korean red ginseng extract (RGE) against cadmium-induced hepatotoxicity in rats. In experiments, animals were orally administrated with RGE (25, 50 mg/kg) for 7 d and then intravenously injected with cadmium (CdCl2, 4 mg/kg) to induce acute hepatotoxicity. Cadmium caused the elevated levels of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase in serum. In contrast, pretreatment with RGE significantly reduced those serum indexes related with liver damage. In histopathological analysis, RGE decreased the centrilobular necrosis around central veins and the peripheral hemorrhage around portal triads. Moreover, RGE restored the deficit in hepatic glutathione level resulting from cadmium treatment. RGE also inhibited the increase in the expression of Bad, a representative apoptosis marker protein, induced by cadmium treatment. Collectively, these results demonstrate that RGE can reduce the cadmium-induced hepatic toxicity, partly via anti-oxidative and anti-apoptotic process.
Park, Sook Jahr,Kim, Young Woo,Park, Moon Ki,Byun, Sung Hui,Kim, Sang Chan,Lee, Jong Rok Springer-Verlag 2016 INFLAMMATION Vol.39 No.2
<P>Lipopolysaccharides (LPS) strongly stimulate immune cells, and unabated activation of immune system by LPS may lead to an exacerbation of sickness and depression. In this study, stigmasta-3,5-dien-7-one (ST) was isolated from Phragmitis rhizoma as a negative regulator of LPS-induced inflammation in macrophages. ST effectively reduced nitric oxide (NO), prostaglandin E-2, and pro-inflammatory cytokine levels, which were markedly raised by LPS treatment. In addition, ST blocked the nuclear factor-kappa B (NF-kappa B) signaling pathway via down-regulation of phospho-p38 mitogen-activated protein kinase and phosphorylation and degradation of the inhibitor of NF-kappa B alpha. To our knowledge, this is the first study showing anti-inflammatory activities of ST isolated from Phragmitis rhizoma.</P>
Protective effects of Korean red ginseng extract on cadmium-induced hepatic toxicity in rats
Park, Sook Jahr,Lee, Jong Rok,Jo, Mi Jeong,Park, Sang Mi,Ku, Sae Kwang,Kim, Sang Chan The Korean Society of Ginseng 2013 Journal of Ginseng Research Vol.37 No.1
Korean red ginseng is known to regulate the immune system and help the body struggle infection and disease. Cadmium is widely distributed in the environment due to its use in industry. Exposure to cadmium is problematic causing organ dysfunction. This study was conducted to evaluate the protective effect of Korean red ginseng extract (RGE) against cadmium-induced hepatotoxicity in rats. In experiments, animals were orally administrated with RGE (25, 50 mg/kg) for 7 d and then intravenously injected with cadmium ($CdCl_2$, 4 mg/kg) to induce acute hepatotoxicity. Cadmium caused the elevated levels of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase in serum. In contrast, pretreatment with RGE significantly reduced those serum indexes related with liver damage. In histopathological analysis, RGE decreased the centrilobular necrosis around central veins and the peripheral hemorrhage around portal triads. Moreover, RGE restored the deficit in hepatic glutathione level resulting from cadmium treatment. RGE also inhibited the increase in the expression of Bad, a representative apoptosis marker protein, induced by cadmium treatment. Collectively, these results demonstrate that RGE can reduce the cadmium-induced hepatic toxicity, partly via anti-oxidative and anti-apoptotic process.
박숙자 ( Sook Jahr Park ),박문기 ( Moon Ki Park ),이종록 ( Jong Rok Lee ) 한국환경과학회 2019 한국환경과학회지 Vol.28 No.11
Chrysanthemum boreale Makino (C. boreale) is widely distributed in Asian countries, and has traditionally been used to treat various inflammatory diseases including bronchitis. In this study, we aimed to isolate biologically active compounds from leaves and stems of C. boreale. Chemical components were purified by column chromatograpy and recyclic HPLC, and characterized from their spectral data (IR, MS, NMR). Biological activity experiments were conducted for Farnesyl-protein transferase (FPTase) activity, apoptosis and nitirc oxide (NO) release. As a results, three sesquiterpene lactones were isolated. Compound 1 (4-methoxy-8-O-acetyl-10-hydroxy-2,11(13)-guaiadiene-12,6-olide) showed strong cytotoxic activities having an average growth inhibition of 50% (GI<sub>50</sub>) value of 1.89 ㎍/㎖against human colon adenocarcinoma cells. Compound 1 also showed a low half maximal inhibitory concentration (IC<sub>50</sub>) value of 10 ㎍/㎖ for NO release. In the caspase 3 activity, compound 1 and compound 2 (8-O-(2-carbonyl-2-butyl)-3,10-dihydroxy-4,11(13) -guaiadiene-12,6-olide) exhibited 94% and 90% apoptosis inhibition activity, respectively. Compound 3 (4,8-O-diacetyl -10-hydroxy-2(3),11(13)-guaiadiene-12,6-olide) showed a strong inhibitory effect on FPTase activity with 90% inhibitory activity at a concentration of 100 ㎍/㎖. These results clearly show the presence of lactone compounds in the leaves and stems, which may partially contribute to the pharmacological activity of C. boreale.