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( Sergiy Oilynyk ),( Seik Wan Oh ) 한국응용약물학회 2012 Biomolecules & Therapeutics(구 응용약물학회지) Vol.20 No.5
Actoprotectors are preparations that enhance body stability against physical loads without increasing oxygen consumption or heat production. Or, in short, actoprotectors are synthetic adaptogens with a significant capacity to improve physical performance. This paper explores the history of actoprotectors` development, their pharmacological properties, mechanism of action, and practical application to the improvement of mental and physical performance. A brief summary of the clinico-pharmacological characteristics of the main representatives of this class (bemitil and bromantane) is provided. Some other synthesized compounds, and even natural ones such as ginseng, also are regarded as potential actoprotectors, and these are treated herein as well. Actoprotectors, owing to their wide-ranging pharmacological activities, high efficiency and safety, can be applied under either normal or extreme conditions.
A case of Pulmonary thrombo-embolism after cesarean section
( Chul Kwon Lim ),( Yun Seok Yang ),( Seik Oh ),( Chan Hee Jin ),( Jin Ho Choi ),( Min Seok Choi ),( Kwan Young Oh ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
The patient was a 31-year-old pregnant woman at 31+4 weeks of gestation with a history of previous Cesarean section, overweight (BMI=28.3) and post-cerclage status. She was hospitalized due to preterm labor and was medicated with tocolytics. On the 10th hospital day, emergency C-section was performed because of rupture of membrane and preterm labor. During operation, nothing unusual was detected. On the 1st day after operation, she complained worsened fatigue with severe dyspnea accompanied by oppressive chest pain. While being uncooperative, the patient's physical examination showed hypotensive, tachycardia, hypoxemia and tachypnea. On ultrasound finding, definite deep vein thrombosis was not detected in both femoral veins, however, on echocardiogram, right ventricle dilatation was severe with D shaped left ventricle. Pulmonary thromboembolism was suspected. Bradycardia and cardiac arrest were checked. After CPR her vital sign was recovery. Therefore, extra corporeal membrane oxygenationI (ECMO) and emergency pulmonary thrombo-embolectomy was conducted. During the operation, small amount thrombi was found in left pulmonary artery. And large amount thrombi was found in right pulmonary artery as well. After the condition improved, the patient was discharged. Pulmonary embolism is one of diseases with the highest mortality. On life threatened condition, emergent thromboembolectomy under ECMO may be an effective management of pulmonary embolism.
Sphingosine Kinase Assay System with Fluorescent Detection in High Performance Liquid Chromatography
Jin, You-Xun,Yoo, Hwan-Soo,Kihara, Akio,Choi, Chang-Hwan,Oh, Seik-Wan,Moon, Dong-Cheul,Igarashi, Yasuyuki,Lee, Yong-Moon The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.11
Activation of Sphingosine kinase (Sphk) increases a bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Therefore, inhibition of Sphk activity was an important target for the development of anticancer drugs. As a searching tool for Sphk inhibitor, we developed fluorescent Sphk activity assay combined with high performance liquid chromatography (HPLC). Previously we established murine teraticarcinoma mutant F9-12 cells which lack S1P lyase and stably express Sphk1. By using F9-12 cells, optimal assay conditions were established as follows; $100\;{\mu}M\;of\;C_{17}-Sph\;and\;30\;{\mu}g$ protein of F9-12 cells lysate in 20 min. Sphingosine analog $C_{17}-Sph$ was efficiently phosphorylated by Sphk activity ($K_{m}:67.08\;{\mu}M,\;V_{max}\;:1507.5\;pmol/min/mg$). New product $C_{17}-S1P$ was separated from S1P in reversed-phase HPLC. In optimized conditions, 300 nM of phorbol 12-myristate 13-acetate (PMA) increased Sphk activity approximately twice while $20\;{\mu}M$ of N,N-dimethylsphingosine (DMS) reduced 70% of Sphk activity in F9-12 cells lysate. In conclusion, we established non-radioactive but convenient Sphk assay system by using HPLC and F9-12 cells.
( Yeon Ju Lee ),( Ji Eun Kim ),( So Yong Jang ),( Seik Wan Oh ) 한국응용약물학회 2013 Biomolecules & Therapeutics(구 응용약물학회지) Vol.21 No.3
This study was aimed at investigating the possible effects of phytoceramide (Pcer) on learning and memory and their underlying mechanisms. Phytoceramide was orally administered to ICR mice for 7 days. Memory performances were assessed using the passive avoidance test and Y-maze task. The expressions of phosphorylated cAMP response element binding protein (pCREB), brain-derived neurotrophic factor (BDNF) were measured with immunoblot. The incorporation of 5-bromo-2-deoxyuridine (BrdU) in hippocampal regions was investigated by using immunohistochemical methods. Treatment of Pcer enhanced cognitive performances in the passive avoidance test and Y-maze task. Immunoblotting studies revealed that the phosphorylated CREB and BDNF were signifi cantly increased on hippocampus in the Pcer-treated mice. Immunohistochemical studies showed that the number of immunopositive cells to BrdU was signifi cantly increased in the hippocampal dentate gyrus regions after Pcer-treatment for 7 days. These results suggest that Pcer contribute to enhancing memory and BDNF expression and it could be secondary to the elevation of neurogenesis.
Sphingosine 1-Phosphate and Sphingosine Kinase Activity during Chicken Embryonic Development
Choi, Chang-Hwan,Jeong, Ji-Seon,Yoo, Bo-Im,Jin, You-Xun,Moon, Dong-Cheul,Yoo, Hwan-Soo,Oh, Seik-Wan,Hong, Seon-Pyo,Lee, Yong-Moon 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.4
The chicken embryo has been weil used in studies of the developmental process, and during development sphingosine and sphingosine 1-phosphate (So1P) are considered critical mediators of cell death and survival. In this study, we compared the sphingolipid contents of chicken embryos during the early embryonic development period from day 3 to day 6. HPLC analyses of sphingosine and So1P in chicken embryos revealed that sphingosine levels were greatly reduced on day 4 whereas So1P levels were not significantly changed. Sphingosine kinase(Sphk) activities, which require sphingosine as substrate to produce So1P, were also greatly reduced on day 4. Collectively, we found sphingosine levels and Sphk activities, but not So1P levels are changed in early stage of chicken embryos development.
Yoo Jae Myung,Lee Youn Sun,Choi Heon Kyo,Lee Yong Moon,Hong Jin Tae,Yun Yeo Pyo,Oh Seik Wan,Yoo Hwan Soo The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.3
Oxidative stress has been reported to elevate ceramide level during cell death. The purpose of the present study was to modulate cell death in relation to cellular glutathione (GSH) level and GST (glutathione S-transferase) expression by regulating the sphingolipid metabolism. LLCPK1 cells were treated with H$_2$O$_2$ in the absence of serum to induce cell death. Subsequent to exposure to H$_2$O$_2$, LLC-PK1 cells were treated with desipramine, sphingomyelinase inhibitor, and N-acetylcysteine (NAC), GSH substrate. Based on comparative visual observation with H202-treated control cells, it was observed that 0.5 $\mu$M of desipramine and 25 $\mu$M of NAC exhibited about 90 and $95\%$ of cytoprotection, respectively, against H$_2$O$_2$-induced cell death. Desipramine and NAC lowered the release of LDH activity by 36 and $3\%$ respectively, when compared to $71\%$ in H$_2$O$_2$-exposed cells. Cellular glutathione level in 500 $\mu$M H202-treated cells was reduced to 890 pmol as compared to control level of 1198 pmol per mg protein. GST P1-1 expression was decreased in H$_2$O$_2$-treated cells compared to healthy normal cells. In conclusion, it has been inferred that H$_2$O$_2$-induced cell death is closely related to cellular GSH level and GST P1-1 expression in LLC-PK1 cells and occurs via ceramide elevation by sphingomyelinase activation.