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( Ji Yeon Byun ),( Hae Young Choi ),( Ki Bum Myung ),( You Won Choi ) 대한피부과학회 2009 Annals of Dermatology Vol.21 No.1
Background: Depending on the light dose and concentration of photosensitizer for photodynamic treatment (PDT), a multitude of dose-related events are demonstrable in PDT-treated cells. Sublethal doses may result in the alteration of cytokine release and consequently modify immune actions, rather than cause cell death. Objective: The purpose of this study was to investigate cytokine expression in cultured HaCaT cells after intense pulse light (IPL) treatment or PDT utilizing 5-aminolevulinic acid (ALA) and IPL at sublethal doses. Methods: Cultured HaCaT cells were treated with either IPL only (4, 8 and 12 J/cm2) or ALA-IPL PDT (100 μmol/L of ALA; 0, 4, 8, and 12 J/cm2 of IPL). The expression of IL-10, TGF-β1 and TNF-α was investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. Results: IL-10 protein increased up to 5.95-fold after IPL treatment and up to 2.85-fold after PDT. TGF-β1 mRNA and protein showed slight increases after both IPL treatment and PDT, of which the latter induced slightly larger increases. TNF-α mRNA and protein showed no induction or reduction after PDT. Conclusion: Increased expressions of IL-10 and TGF-β1 was observed after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses, and that of TGF-β1 may be related to the therapeutic effect for psoriasis. The finding that IL-10 induction was more marked after IPL treatment than after PDT suggests that other mechanisms than IL-10 induction in keratinocytes after PDT may participate in the anti-inflammatory effect of PDT. (Ann Dermatol(Seoul) 21(1) 12∼17, 2009)
Doubling Time of Serum Tumor Marker in HCC Patients Predicts Recurrence after Curative Treatment
( Ji Hye Je ),( Yang Jae Yoo ),( Young-sun Lee ),( Sang Jun Suh ),( Young Kul Jung ),( Ji Hoon Kim ),( Yeon Seok Seo ),( Hyung Joon Yim ),( Jong Eun Yeon ),( Kwan Soo Byun ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: Alpha-fetoprotein (AFP) and protein-induced vitamin K absence (PIVKA-II) have been used as a marker for hepatocellular carcinoma (HCC). We aimed to investigate the correlation of doubling time (DT) of tumor markers (AFP, PIVKA-II) after curative treatment with recurrence of HCC. Methods: Between January 2005 and December 2013, total of 451 patients were enrolled. After excluding the patient with loss to follow- up, tumor remnant and absence of repeated tumor marker, 213 patients who received curative treatment were analyzed (Liver transplantation, surgical resection, radiofrequency ablation, percutaneous ethanol injection). Serum AFP and PIVKA II levels before and after the curative treatment were collected and used for analysis. Tumor marker DT was calculated as DT=t log 2/(log (marker 2nd)-log (marker 1st)) where t was the time interval between from nadir level after treatment to level at recurrence or 2 years later after treatment. Results: Mean age was 56 years. HBV infection was the most common etiology of HCC (78.4%), and most of the patients were in BCLC stage 0 and A (77.9%). During the follow up, 127 of 213 patients (59.6%) were recurred. Median DT of AFP and PIVKA-II was 20months and 18.7months. In comparing the HCC patients with or without early recurrence, DT was significantly lower in the early recurrence group (AFP and PIVKA II: 10.4 vs 26.78 months; P<0.001 and 8.75 vs 24.67 months; P<0.001, respectively). In univariate analysis, BCLC stage, tumor size, PIVKA-II level at diagnosis, AFP DT and PIVKA-II DT were significantly related to recurrence rate. In multivariate analysis, AFP DT and PIVKA-II DT and BCLC stage at diagnosis were only independent predictor of recurrence after curative treatment on HCC. Conclusions: In HCC patients with curative treatment, the DT of AFP and PIVKA-II from nadir level after the treatment is useful tools to predict early recurrence.
( Ji Hye Je ),( Ji Hoon Kim ),( Yang Jae Yoo ),( Sung Hee Kang ),( Hae Rim Kim ),( Sang Jun Suh ),( Young Kul Jung ),( Yeon Seok Seo ),( Hyung Joon Yim ),( Jong Eun Yeon ),( Kwan Soo Byun ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Several staging systems for hepatocellular carcinoma (HCC) have been purposed but there is no worldwide consensus which staging system is best. We identifi ed the best staging system evaluating the predictive ability for outcome in patients with hepatocellular carcinoma in Korea. Methods: Between January 2004 and December 2009, total of 875 patients with HCC who were diagnosed and treated at the Korea University Guro hospital were enrolled. They were followed up till April 2014. All patients were classifi ed by modifi ed UICC, Barcelona-Clinic Liver Cancer (BCLC), Cancer of the Liver Italian Program (CLIP), Chinese University Prognostic Index (CUPI), Japan Integrated Staging (JIS), Tokyo score. The demographic data, clinical variables, tumor characters, residual liver function and initial treatment modalities were reviewed. The discriminatory ability of staging system was quantifi ed using C-index. And the homogeneity and monotonicity of staging system was assessed using likelihood ratio χ2 test correlated with a Cox`s regression model neutralized by Akaike information criterion (AIC). Results: 654 (74. 7%) patients died and the median survival time was 25 months. The 5-years survival was 28. 1%. There were significant differences between all stage in modifi ed UICC, BCLC, CLIP, CUPI, JIS, Tokyo score. In the overall group of patients, CLIP stage had higher discrimination ability (C-index 0. 76) and the best monotonicity of gradient and homogeneity ability (LR χ2 test : 319. 541, P < 0. 008, AIC : 7789. 566). Also JIS system had higher discrimination ability (C-index 0. 75) and higher monotonicity of gradient and homogeneity ability (LR χ2 test : 320. 356, P<. 0001, AIC : 7792. 751). Conclusions: The CLIP and JIS stage systems showed the best prognostic stratifi cation in HCC patients of Korea.
Age-Related Changes of Adult Neural Stem Cells in the MouseHippocampal Dentate Gyrus
Ji-Yeon Jung,Kang-Ok Byun,Yeon-Jin Jeong,Won-Jae Kim KOREAN ACADAMY OF ORAL BIOLOGY 2008 International Journal of Oral Biology Vol.33 No.2
This study was designed to investigate the changes in the properties of the neuronal setm cells or progenitor cells associated with age-related decline in neurogenesis of the hippocampal dentate gyrus (DG). Active whole cells cycle marker Ki67 (a marker of whole cell cycle)-positive and S phase marker bromodeoxyuridine (BrdU)-positive. Neural stem cells gradually were reduced in the hippocampal subgranular zone (SGZ) in an age-dependant manner after birth (from P1 month to P1 year). The ratio of BrdUpositive cells/Ki67-positive cells was gradually enhanced in an age-dependent manner. The ratio of Ki67-positive cells/accu-mulating BrdU-positive cells at 3 hrs after BrdU injection was injected once a day for consecutive 5 days gradually decreased during ageing. TUNEL- and caspase 3 (apoptotic terminal caspase)-positive cells gradually decreased in the dentate SGZ during ageing and immunohistochemical findings of glial fibrillary acid protein (GFAP) were not changed during ageing. NeuN, a marker of mature neural cells, and BrdU-double positive cells gradually decreased in an age-dependent manner but differentiating ratio and survival rate of cells were not changed at 4 wks after BrdU injection once a day for consecutive 5 days. The number of BrdU-positive cells migrated from the hippocampal SGZ into granular layer and its migration speed was gradually declined during ageing. These results suggest that the adult neurogenesis in the mouse hippocampal DG gradually decrease through reducing proliferation of neural stem cells accompanying with cells cycle change and reduced cells migration rather than changes of differentiation.
The Expressions of TGF-β1 and IL-10 in Cultured Fibroblasts after ALA-IPL Photodynamic Treatment
( Ji Yeon Byun ),( Ga Youn Lee ),( Hae Young Choi ),( Ki Bum Myung ),( You Won Choi ) 대한피부과학회 2010 Annals of Dermatology Vol.23 No.1
Background: Topical photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) was originally used for treating superficial skin tumors. The application of PDT to other inflammatory dermatoses like acne vulgaris, psoriasis, granuloma annulare, localized scleroderma and lichen sclerosus has recently been introduced. However, the underlying mechanisms are not well understood. We`ve previously reported the induction of tumor growth factor (TGF)-β1 and interleukin (IL)-10 after PDT with ALA and intense pulsed light (IPL) in cultured HaCaT cells. Objective: The purpose of this study was to investigate the expressions of TGF-β1 and IL-10 in cultured fibroblasts after PDT with using ALA and IPL. Methods: Cultured fibroblasts were treated with ALA-IPL PDT (1umol/L of ALA; 0, 4, 8 and 12 J/cm2 of IPL). The expressions of TGF-β1 and IL-10 were investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. Results: TGF-β1 mRNA and protein were reduced down to 0.52- and 0.63-fold, respectively, after PDT and the IL-10 protein was increased up to 2.74-fold after PDT. Conclusion: The reduction of TGF-β1 was prominent after PDT and so an antisclerotic effect can be expected after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses. (Ann Dermatol 23(1) 19~22, 2011)
Case Reports : A Case of Dedifferentiated Liposarcoma That Developed in the Dermis
( Ji Yeon Byun ),( You Won Choi ),( Hae Young Choi ),( Ki Bum Myung ) 대한피부과학회 2008 Annals of Dermatology Vol.20 No.4
Dedifferentiated liposarcoma is a variant of liposarcoma, and this is characterized by the coexistence of well-differentiated liposarcoma with areas of poorly differentiated, non-lipogenic tumor and this is also known to be associated with more aggressive behavior. Dedifferentiated liposarcoma occurs principally in the retroperitoneum or the deep soft tissue of limbs, but it can also occur in subcutaneous locations. We report here on a peculiar case of dedifferentiated liposarcoma that developed in the dermis, which is an exceedingly rare location for this type of tumor. The occurrence of this tumor in the dermis made it easy to surgically remove and monitor for recurrence, and we expect this patient to have a better prognosis than that of a patient with dedifferentiated liposarcoma located in the retroperitoneum or deep soft tissue. (Ann Dermatol (Seoul) 20(4) 204∼208, 2008)