신생아 고 17α-Hydroxyprogesterone치의 추이

박은영,조진형,이수진,양승,김광남,신재훈,오필수 대한소아내분비학회 2003 Annals of Pediatirc Endocrinology & Metabolism Vol.8 No.2

Sulfhydryl기와 세포막 구성성분의 대사 변화에 따른 다형핵 백혈구 기능의 변경

신재훈(Jeh-Hoon Shin),이정수(Chung-Soo Lee),한은숙(Eun-Sook Han),신용규(Yong-Kyoo Shin),이광수(Kwang-Soo Lee) 대한약리학회 1989 대한약리학잡지 Vol.25 No.1

면역 보체가 결합되어 있는 zymosan에 의하여 활성화된 다형핵 백혈구에서 세포 투과성 물질인 N-ethylmaleiamide과 Hg⁺⁺은 superoxide 라디칼 생성, NADPH oxidase 활성도 및 lysosomal enzyme (lactic dehydrogenase, β-glucuronidase)의 유리를 억제하였다. 세포막 단백에 특이적인 p-chloromercuribenzoic acid와 p-chloromercuribenzenesulfonic acid는 superoxide 라디칼 생성에 영향을 주지 않았으나 NADPH oxidase 활성도와 lysosomal enzyme의 유리를 억제하였다. 식작용 중에 세포막과 세포내의 sulfhydryl기는 반응시간에 따라 점진적으로 감소하였다. N-ethylmaleiamide와 Hg⁺⁺은 세포막과 세포내의 sulfhydryl기를 모두 감소시켰다. P-Chloromercuribenzoic acid와 p-chloromercuribenzenesulfonic acid는 세포막의 sulfhydryl기를 유의하게 감소시켰으나 세포내 용해성 sulfhydryl기에는 영향을 주지않았다. Cysteine과 mercaptopropionylglycine는 superoxide 라디칼의 생성과 lysosomal enzyme의 유리를 억제하였다. Gluthathione은 superoxide생성에 영향을 주지 않았으나 뚜렷하게 lactic dehydrogenase의 유리를 억제하였다. N-ethylmaleiamide에 의한 superoxide 생성의 억제는 cysteine과 mercaptopropionyl-glycine에 의하여 반전되었으나 gluthathione의 영향은 없었다. N-ethylamleiamide에 의한 NADPH oxidase의 비활성화는 gluthathione, cysteine과 mercaptopropionylglycine에 의하여 저해되었다. Carbachol에 의하여 항진된 superoxide 라디칼 생성은 N-ethylamleiamide에 의하여 완전히 억제되었고, atropine에 의하여 길항되었다. 그러므로, 외부 자극에 대한 다형핵 백혈구 반응의 표현은 sulfhydryl기의 양의 변화와 연관이 있을 것으로 시사되었다. Lysosomal enzyme 유리는 세포막과 세포내의 sulfhydryl기에 의하여, 이에 반하여 superoxide생성은 세포내 sulfhydryl기에 의해서 영향받을 것으로 추정되었다. In opsonized zymosan activated PMN leukocytes, N-ethylamleiamide and Hg⁺⁺, penetrable sulfhydryl group inhibitors, inhibited superoxide generation, NADPH oxidase activity and lysosomal enzyme (lactic dehydrogenase and β-glucuronidase) secretion. P-Chloromercuribenzoic acid and p-chloromercuribenzenesulfonic acid, surface sulfhydryl group inhibitors did not affect superoxide generation but effectively inhibited both NADPH oxidase activity and lysosomal enzyme secretion. During phagocytosis, contents of surface and soluble sulfhydryl groups were gradually decreased with increasing incubation times. N-ethylmaleiamide and Hg⁺⁺ caused a loss of both surface and soluble sulfhydryl groups. P-Chloromercuribenzoic acid and p-chloromercuribenzenesulfonic acid significantly decreased the surface sulfhydryl content but did not after soluble sulfhydryl groups. Cysteine and mercaptopropionylglycine inhibited superoxide generation and lysosomal enzyme secretion. Glutathione had no effect on superoxide generation but remarkably inhibited lactic dehydrogenase release. Suppression of superoxide generation by N-ethylmaleiamide was reversed by cysteine and mercaptopropionyl-glycine but not by glutathione. Inactivation of NADPH oxidase by N-ethylmaleiamide was prevented by glutathione, cysteine or mercaptopropionylglycine. Stimulated superoxide generaion by carbachol was completely abolished by N-ethylrnaleiamide and antagonized by atropine. Thus, the expression of PMN leukocyte response to external stimuli may be associated with the change of sulfhydryl groups content. It is suggested that lysosomal enzyme secretion is influenced by both surface and soluble sulfhydryl groups, whereas superoxide generation by intracellular soluble sulfhydryl groups.

선천성 갑상선종성 기능저하증

신재훈 한국의학사 1989 診斷과治療 Vol.9 No.8

열량제한 백서에서 1 형 인슐린양 성장인자의 효과 : 1 형 인슐린양 성장인자 투여시 혈중농도를 정상으로 회복하나 장기 저항에 관한 연구 1 injection to promote growth in energy restricted rats despite normalization of serum IGF - 1 concentration

신재훈 대한내분비학회 1994 Endocrinology and metabolism Vol.9 No.3

In addition to regulation mediated by CH, nutritional status is an important regulator of IGF-Ⅰ, circulating levels of these peptides modulated by poor nutritional status and poorly controlled insulin dependent diabetes mellitus(IDDM). Impaired growth is a well recognized complication of poorly controlled diabetes. This study was designed to examine the anabolic effect of recombinant human IGF-I in energy-restriction model. Experimental design; Sprague-Dawley rats(n=20) weighing 90-100g were used. Rats were fed a control diet two times a day(AM 8-11, PM 5-8) for four days after arrival and then assigned to one of three groups: control, energy-restricted, energy-restricted IGF-I treatment group. Energy restricted group was given with a decrese of 25% in the energy without changes in the protein by feeding 88% by weight to energy-restricted diet. During the 10days of energy restriction, the growth rate was reduced by 35%(2.70+-0.18g/day in energy restricted group vs. 4.13+-0.75g/day in the control group). At sacrifice, the tail lengh and weight of organs were not significantly decreased except the spleen and thymus(-17%: P$lt;0.05). Serum IGF-I was reduced by 19% at the end of 10days of energy restriction. The glycemia, measured each day by glucometer from blood collected at the tail, was not reduced by energy restriction(105.4+-7.7 in control group vs. 101.3+-4.1㎎/㎗). The abundance of serum IGF-BPs was unchanged by this restriction. Despite the 1.5 fold increase of IGF-I concentration in energy restricted IGF-I injection group at sacrifice(1994+-172ng/㎖ vs. 1221+-110 ng/㎖ energy restricted group), IGF-I treatment(300 ㎍/day in twice sc injection for 6day) did not significantly accelerate the growth rate(body weight)(2.87+-0.20 vs. 2.70+-0.18g/day in energy restricted group). The glycemia was slightly reduced by IGF-I treatment(91.7+-5.0 ㎎/㎗ vs. 101.3+-4.5 ㎎/㎗ in energy restricted group), but it was not significant. However, the spleen and thymus weight, decreased by energy restriction, was completely normalized by IGF-I treatment. In summary, lack of a significant anabolic response to injection of IGF-I during energy restriction in this study may be associated with the compensatory growth response(alterations in dietary protein utilization) which followed initial period of energy restriction(J Kor Soc Endocrinol 9: 213-218, 1994).

고압 액상 크로마토그래피를 이용한 혈중 성장홀몬-결합단백 측정에 대한 초기보고

신재훈 대한내분비학회 1993 Endocrinology and metabolism Vol.8 No.4

A technique high pressure liquid chromatography (HPLC) gel filtration was used to evaluate GH-binding proteins in human serum. Seurm (75 l) was well seperated from free Biotin-hGH(PeakIII). Elution profile was monitored in potical density multisaner system. In chromatography, the molecular weight of Peak I and II appeared about 200 K and 60 K dalton respectively. In control serum, the specific binding of biotin-HGH to Peak II-BP was 59.7+/-0.8% of the optical density. Specific binding of Biotin hGH to Peak Ii-BP was low during the infant period and progressively increase as age. Using gel filltration system, binding of biotin-hGH by normal adult serum was dependent on time (maximum equlibrium was reached in 24h at 4C), temperature( 4C>22C>37C)and seurm concentration. These data indicated that human sera contain a specific binding protein for hGH. The biological significance of this binding protein remains to be investigated. We sugest the measurement of GH-BP II activity by HPLC provides a clear seperation. Our nonradioactive optical detection method by multiscaner was also highly resolution (J Kor Soc Endocrinol 8: 432438, 1993)

임상영양지표 ( Nutritional Index )로서의 1형 인슐린양 성장인자 ( IGF-I )의 역할

신재훈 대한내분비학회 1994 Endocrinology and metabolism Vol.9 No.2

저신장의 평가와 성장호르몬 치료대상

신재훈 대한가정의학회 1993 Korean Journal of Family Medicine Vol.14 No.3

선천성 부신 과형성

신재훈 한국의학사 1989 診斷과治療 Vol.9 No.5

성장지연의 진단 및 치료

신재훈 한국의학사 1988 診斷과治療 Vol.8 No.10

In vitro Folding of Recombinant Hepatitis B Virus X-Protein Produced in Escherichia coli: Formation of Folding Intermediates

Lee,Young Ik,Shin,Jeh-Hoon,Jeong,Soon-Seog,Kim,Sun-Ok,Sohn,Mi Jin The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The folding of recombinant hepatitis B virus X-protein (rHBx) solubilized from Escherichia coli inclusion bodies was investigated. By sequential dialysis of urea, rHBx was folded into its native structure, which was demonstrated by the efficacy of its transcriptional activation of the adenovirus major late promoter (MLP), fluorescence spectroscopy, and circular dichroism (CD) analysis. The decrease in CD values at 220 nm and a corresponding blue shift of the intrinsic fluorescence emission confirmed the ability of rHBx to refold in lower concentrations of urea, yielding the active protein. Equilibrium and kinetic studies of the refolding of rHBx were carried out by tryptophan fluorescence measurements. From the biphasic nature of the fluorescence curves, the existence of stable intermediate states in the renaturation process was inferred. Reverse phase-high performance liquid chromatography (RP-HPLC) analysis further demonstrated the existence of these intermediates and their apparent compactness.