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Lim, Jong-Hui,An, Chang-Hwan,Kim, Yo-Hwan,Jung, Byung-Kwon,Kim, Sang-Dal The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.5
Plant tissues produce ethylene under the environmental stresses such as drought, salinity, and heavy metals. Ethylene concentration can be reduced by 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase produced by plant growth-promoting rhizobacterium (PGPR), which cleaves the ethylene precursor ACC. The present study focused on alleviation of environmental stress by selected PGPR, which could suppress fungal plant disease. These PGPRs were capable of utilizing ACC as sole source of nitrogen and also produced auxin. Seed germination of red pepper was reduced with increasing salt concentration, and approximately 98.2% of seeds germinated in the absence of salt, whereas only 36.2% seeds germinated in the presence of 175 mM NaCl. Seed germination was also decreased by 62.1 and 19.9% in the presence of 120mM NaCl and 120mM NaCl+ACC deaminase-producing PGPR Pseudomonas fluorescens 2112, respectively, compared to uninoculated control. The effect of salinity stress with different salt concentration on pepper plants and their alleviation with PGPR was evaluated. Non-inoculated pepper plants died after 5 week when grown in the presence of high salt (120 mM NaCl), whereas 80% of pepper plants inoculated with P. fluorescens 2112 survived under the high salt stress. Salt stress also decreased the fresh and dry weights of pepper grown, compared to the negative control, whereas pepper plants inoculated with P. fluorescens 2112 retained the biomass similar to control plants. These results indicate that ACC deaminase and auxin producing P. fluorescens 2112 is a multi-functional PGPR that can promote the growth and development of pepper plants by alleviating the high-salt stress.
Lim, Jong-Hui,Ahn, Chang-Hwan,Jeong, Hee-Young,Kim, Yo-Hwan,Kim, Sang-Dal The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
The plant growth promoting rhizobacteria (PGPR) strains Bacillus subtilis AH18 and Bacillus licheniformis K11 were selected as biocontrol agents for the suppression of phytophthora blight caused by Phytophthora capsici. A genetic monitoring method was developed utilizing multiplex and real-time polymerase chain reaction (PCR) in a pepper farming soil. 2,3-Dihydro-2,3-dihydroxy benzoate dehydrogenase of a key siderophore synthesis enzyme (sid), auxin efflux carrier gene (aec), and cellulase gene (cel) of B. subtilis AH18 were used as genetic methods to monitor the presence and concentration of the inoculated microbial agents. Monitoring of B. licheniformis K11 was carried out by amplification of a cellulase gene (celK), siderophore synthase gene (dhbF), and iturin synthase A gene (ituA). B. subtilis AH18 and B. lichenifomis K11 could be detected upto 20 days by multiplex PCR in pepper farming soil. B. subtilis AH18 sid and three Bacillus lichenifomis K11 genes could be detected upto week 5 by real-time PCR in the natural unsterilized soil. P. capsici pathogen became nearly undetectable after 20 days biocontrol treatment in the field soil. These results could lead to the development of select PGRPs as useful microbial agents for the organic farming of peppers.
( Jong Hui Lim ),( Chang Hwan Ahn ),( Hee Young Jeong ),( Yo Hwan Kim ),( Sang Dal Kim ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
The plant growth promoting rhizobacteria (PGPR) strains Bacillus subtilis AH18 and Bacillus licheniformis K11 were selected as biocontrol agents for the suppression of phytophthora blight caused by Phytophthora capsici. A genetic monitoring method was developed utilizing multiplex and real-time polymerase chain reaction (PCR) in a pepper farming soil. 2,3-Dihydro-2,3-dihydroxy benzoate dehydrogenase of a key siderophore synthesis enzyme (sid), auxin efflux carrier gene (aec), and cellulase gene (cel) of B. subtilis AH18 were used as genetic methods to monitor the presence and concentration of the inoculated microbial agents. Monitoring of B. licheniformis K11 was carried out by amplification of a cellulase gene (celK), siderophore synthase gene (dhbF), and iturin synthase A gene (ituA). B. subtilis AH18 and B. lichenifomis K11 could be detected upto 20 days by multiplex PCR in pepper farming soil. B. subtilis AH18 sid and three Bacillus lichenifomis K11 genes could be detected upto week 5 by real-time PCR in the natural unsterilized soil. P. capsici pathogen became nearly undetectable after 20 days biocontrol treatment in the field soil. These results could lead to the development of select PGRPs as useful microbial agents for the organic farming of peppers.
다중 검출 전산화단층촬영을 이용한 국내 일부 지역 성인의 골밀도 현황 분석: 식습관, 생활습관, 신체적, 사회적 특성과의 상관관계를 중심으로
이태희(Tae Hui Lee),김태형(Tae-Hyung Kim),소운영(Woon Young So),임희겸(Hei Gyeom Lim),임청환(Cheong-Hwan Lim),박명환(Myeong Hwan Park),천명기(Myung-Ki Cheoun) 대한방사선과학회(구 대한방사선기술학회) 2016 방사선기술과학 Vol.39 No.4
다중 검출 전산화단층촬영(Multi detecter computed tomography; MDCT)에서 계산된 골밀도 값과 대상 검사자 의 생활양식, 신체적 특성, 사회적 특성과의 상관관계를 분석하고자 하였다. 2015년 7월 15일부터 2016년 6월 6일까지 00의료원에 내원한 검사자 141명(남: 63명, 여: 78명)을 대상으로 MDCT를 시행하여 HU값을 골밀도의 T-score 값으로 환산하였다. 골밀도를 측정한 대상 부위는 요추 두 번째, 세 번째 그리고 네 번째 요추이었으며, 남녀별 골밀도 차이와 검사자의 생활양식, 신체적 특성, 사회적 특성과의 상관 관계를 분석하였다. 통계적 유의성 검증은 독립표본T검정과 일원배치 분산분석법을 사용하였다. 남녀의 골밀도는 통계적으로 유의한 차이가 있음을 확인하였고(p<0.05), 연령별 골밀도 값은 연령이 증가함에 따라 평균 골밀도 값 이 감소하였지만 통계적으로는 남성의 경우는 20대부터 50대까지 유의한 차이가 없었고(p>0.05), 20대와 60대 이 상의 연령에서만 유의한 차이를 보였다(p<0.001). 여성의 경우는 20대부터 40대까지 유의한 차이가 없었으며 (p>0.05), 50대 이후로 골밀도가 급격히 감소하며 유의한 차이를 보였다(p<0.001). 여성인 경우만이 월경과 폐경, 출산, 음주 유무와 잡곡류, 계란, 기름진 음식 섭취 유무에 대하여 골밀도 변화의 유의한 차이를 보였고(p<0.05), 남성의 경우는 유의한 차이가 없었다. MDCT를 이용한 골밀도 측정법으로 검사자의 생활양식, 신체적 특성, 사회적 특성과의 상관관계를 추정할 수 있었고, 골다공증 관리를 위한 기초자료로 활용이 가능할 것으로 사료된다. This study analyzed the correlation between BMD (bone mineral density) value calculated in the MDCT (multidetector computed tomography) and lifestyle, physical features and social characteristics. From July 15 2015 to June 6 2016, we converted from HU (hounsfield unit) value measured by using MDCT to T-score for BMD of 141 patients (male: 63, female: 78) in W medical center. We measured the 2nd, 3rd and 4th lumbar spine and analyzed the correlation between gender differences in BMD and lifestyle, physical features and social characteristics. Statistical significance was validated using independent sample T test with one way Anova. Gender BMD was confirmed that a statistically significant difference (p<0.05). BMD values decreased with increasing age but for the statistically men, there was no significant difference from 20s to 50s, it only showed a significant difference in 20s and 60s (p<0.001). For the statistically women, there was no significant difference from 20s to 40s. but since 50s BMD was decreased rapidly, which showed a significant difference (p<0.001). women showed significant differences for the menstruation and menopause, childbirth, alcohol, cereals and greasy food in bone mineral density (p<0.05) but there were no significant differences in men. The bone mineral density values calculated by the MDCT and lifestyle, physical features and social characteristics correlation analysis method is considered to be used as a basis for estimating the state in BMD and osteoporosis management.
Lim Eun Hee,Kim Da-Sol,Won Yu-Hui,Park Sung-Hee,Seo Jeong-Hwan,Ko Myoung-Hwan,Kim Gi-Wook 대한뇌신경재활학회 2023 뇌신경재활 Vol.16 No.1
Mild cognitive impairment (MCI) increases with aging society. Serious games may be effective in improving cognitive function in patients with MCI; however, research on their effects remains insufficient. This study aimed to confirm the efficacy and safety of cognitive rehabilitation training using a serious game (Brain Talk™) for the elderly with MCI. Twenty- four elderly individuals with MCI were randomized into study and control groups. The study group received 12 training sessions (30 min/session, 3 times/week), whereas the control group did not receive training. Blinded evaluations were conducted before and after the training and four weeks after the training. The primar y outcome measures were the Korean Mini-Mental State Examination (K-MMSE) and K-MoCA (Korean Montreal Cognitive Assessment). Secondar y outcome measures were the Semantic Verbal Fluency Task (SVFT), Trail-Making Test-B, and 2-back test. In the study group, the K-MMSE, K-MoCA, and SVFT scores after finishing the training and 4 weeks after training showed a significant increase; however, there was no significant change in the control group. No significant differences were obser ved between the two groups. Cognitive function significantly improved in the study group after training. Home-based serious games are considered helpful in improving cognitive function
Lim, Jong-Ok,Oh, Myeong-Hui,Lee, Jong-Wook,Lee, Seung-Hwan Korean Society of Applied Entomology 2007 Journal of Asia-Pacific Entomology Vol.10 No.4
An ectoparasitoid natural enemy, Cephalonomia gallicola (Ashmead 1887) (Hymenoptera: Bethylidae) was found in the mass rearing colonies of cigarette beetle, Lasioderma serricorne (Fabricius 1792) (Coleoptera: Anobiidae), under laboratory condition. As the first record in Korea, the description of the apterous female is provided for the macerated microslide specimens, with the detail illustration for the taxonomic characteristics and the biometric data.
( Yun Hwan Kim ),( Hyun-jung Kim ),( Young-mee Lim ),( Jing Hui Jin ),( Chan Young Kim ),( Woong Ju ),( Seung Cheol Kim ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
목적: Although the Papanicolaou smear (Pap smear) and HPV DNA testing have greatly reduced the mortality and morbidity for patients with invasive cervical cancer, low sensitivity and specificity of Pap smear and HPV DNA typing, respectively, still make a need to develop other biomarkers for cervical cancer screening. DNA methylation has been spotlighted as a potential biomarker for the early diagnosis of cancers and also as a means of assessing the prognosis for patients with cancers. Here, this study was aimed at investigating the single-minded homolog 1 (SIM1) promoter methylation and its clinical relevance in cervical cancer. 방법: Using real-time quantitative methylation-specific polymerase chain reaction (PCR) (qMSP), we analyzed the frequency and relative level of promoter methylation of SIM1 gene in 45 cervical cancer tissues and 10 normal controls. The percentage of methylated references (PMR) was used for analysis of SIM1 methylation using reference gene, ACTB. 결과: A much higher level of aberrant SIM1 methylation was measured in most cervical cancers (42/45, 93.3%), compared with normal control (0/10, 0%), and PMR values for SIM1 have ranges of 0-100 (median PMR 80.9) and 0-3.3 (median PMR 0) in tumor tissues and controls, respectively (cutoff >7.03, P < 0.0001). Aberrant SIM1 methylation showed no statistically significant difference between stage I and II, but a trend of stage-dependent increase in SIM1 hypermethylation was show. To see whether aberrant SIM1 methylation in tumor was from germline methylation of SIM1 gene, we investigated SIM1 methylation in patient-derived peripheral blood leucocytes (PBL). Among nineteen PBL, only two have very low SIM1 methylation and PMR values of SIM1 in PBL have ranges 0-6.26 (median PMR 0, >7.03 cutoff), indicating that SIM1 methylation in tumor tissues might be somatic methylation during carcinogenesis. 결론: Taken together, our data suggest that aberrant SIM1 methylation may be a promising biomarker for early diagnosis of cervical cancers.
열중량분석을 활용한 21MPa급 콘크리트의 화해피해깊이 진단방법 분석
임군수 ( Lim Gun Su ),한수환 ( Han Soo Hwan ),한준희 ( Han Jun Hui ),윤치환 ( Yoon Chee Whan ),한민철 ( Han Min Cheol ),한천구 ( Han Cheon Goo ) 한국건축시공학회 2021 한국건축시공학회 학술발표대회 논문집 Vol.21 No.1
In this study, we develop diagnostic technology for damage depth of fire-damaged concrete and propose methods for damage caused by fire by drying damaged concrete after immersion. As a result of the test, the damaged depth was clearly found when evaluating the depth of impurities caused by fire in a drying method after water had permeated, and it could be verified using thermogravimetric analysis.