Algal bioassay 에 의한 조류생장 제한영양염류 결정

오희목(Hee Mock Oh),이석준(Seog June Lee),김성빈(Seong Bin Kim),박미경(Mi Kyung Park),윤병대(Byung Dae Yoon),김도한(Do Han Kim) 한국하천호수학회 1998 생태와 환경 Vol.31 No.2

Determination of Limiting Nutrient for Algal Growth by Algal Bioassay. Oh, Hee-Mock, Seog June Lee, Seong-Bin Kim, Mi- Kyung Park, Byung-Dae Yoon and Do-Han Kim (Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea and Water Resources Research Institute, Korea Water Resources Corporation, Taejon 305-390, Korea) Water quaiity and algal growth potential (AGP) using Microcystis aeruginosa were investigated from April to September 1997 in Daechung Reservoir. Secchi disk transparency was 1.1 m in August. Chlorophyll-a concentrations were higher than 10㎍/l at all samples. Total phosphorus concentration was in a range of 0.020∼0.027mg/l which is at the approximate value of 0.03 mg/l, the general critical level of eutrophication. These results indicate that Daechung Reservoir is in an eutrophic state except some temporary times and restricted sites. Phosphorus uptake rate of M. aeruginosa was higher in the water from the epilimnion than in the hypolimnion and also higher under light condition than under dark condition. Therefore, phosphorus uptake rate appears to depend on cellular phosphorous content and light condition, a meaning of endergonic process. Phosphorus was the most frequently limiting nutrient: phosphorus was limiting algal gowth in April and May, nitrogen, phosphorus and trace elements were the limiting factors for algae in August, and trace elements in September. If a fast growing alga, M, aeruginosa, is used as a test organism for assaying AGP, in vivo fluorescence instead of dry weight can be measured for algal biomass and incubation period can be reduced from traditional 14 days to 7 days.

해산어 양식수중의 암모니아성 질소의 오존분해에 관한 실험실적 연구

허목,오희부 제주대학교 해양연구소 1998 해양자원연구소연구보고 Vol.22 No.-

The dissolution and reaction of ozone in sea water with bromide has been tested. In order to obtain the optimal amount of injected ozone which is needed for the oxidation of ammonia in sea water, dissolved ozone concentration and temperature have changed into three conditions, which are 1, 1.5. 2.5 mg/L and 15, 20, 23℃ respectively. In this study, Ammonium nitrogen has been injected with different concentration of 0.5. 1, 1.5 and 2mg/L at each Run and at Run 3 which has high dissolved ozone concentration. 5 and 10mg/L of NH_(4)-N has been added. pH has decreased rapidly in ten minutes in accordance with denitrification of ammonium nitrogen. With increasing ammonium nitrogen concentration, it has decreased significantly within five minutes and then slowly. In the rate of 1-2.5mg/L of injected ozone concentration, the removal efficiency of ammonium nitrogen has increased clearly with increasing ozone concentration. In the course of denitrification, the variation of nitrate nitrogen and nitrite nitrogen has scarcely been measured. This is considered that ammonium nitrogen has been denitrified by a complicated mechanism rather than by a simple removal through its oxidation. and has been emitted into the air as nitrogen gas in a short time.

호밀 (Secale cereale L.)유식물의 질산환원효소의 활량과 단백질함량에 관한 Cycloheximide의 영향

권오용,이영하,오희목,박흥순,이진철 충남대학교 자연과학연구소 1988 忠南科學硏究誌 Vol.15 No.2

This work was accomplished with 6 day-old rye (Secale cereale L.) seedlings to determine the effect of the antibiotics by cycloheximide on in vivo nitrate reductase activity, protein and RNA content. The nitrate reductase activity by treating 0.1μM and 0.1 mM cycloheximide was lower than that of the control group. The optimum pH for the nitrate reductase of the control group was 7.6 in the range of pH 5.6-8.0, but, in the case of 0.1mM cycloheximide treatment group, the optimum pH was decreased to 6.8 within the weak the acid region. The protein content of shoot and root in the 0.1μM and 0.1 mM cycloheximide treatment groups were respectively found at the 96%, 82% level of that in the control group. In the higher cycloheximide treatment (0.1 mM CH), not only nitrate reductase activity and protein content but also RNA content was greatly decreased. In this fact, it was suggested that the 0.1 mM cycloheximide had affected on the RNA polymerase. On the basis of there results, it was concluded that the decreased nitrate reductase activity by treating cycloheximide occurred primarily according to the decreased nitrate uptake, and the decreased protein and RNA content depended upon the results of the direct and indirect effect of cycloheximide on the protein synthesis.

금강유역 수질오염 실태조사

최신석,오희목,강명규,조통래,이규승,유종국,이영하 忠南大學校 環境問題硏究所 1988 環境硏究 Vol.6 No.1

조사 구역 상류 지점의 평균 수질은 pH가 7.1, DO가 8.3mg/ℓ, BOD가 1.7mg/ℓ, COD가 1.7mg/ℓ, SS가 15.4mg/ℓ, 분변성 대장균이 1.1x10³ MPN/100ml고 상수원 수 1급 내지 2급의 양호한 상태이고 하류지점의 수질은 pH가 6.8, DO가 7.7mg/ℓ, BOD가 2.2mg/ℓ, COD가 2.4mg/ℓ, 분변성 대장균이 1.7x10³MPN/100ml로 상수원 수 2급 내지 3급의 상태를 나타내 하류로 내려가면서 오염도가 증가하는 것으로 나타났다. 청주시의 영향을 크게 받는 미호천 하류의 평균수질은 pH가 7.2, DO가 6.3mg/ℓ, BOD가 16.2mg/ℓ, COD가 15.1mg/ℓ, 분변성 대장균이 1.6x10??MPN/100ml로 심각한 오염 상태를 보여 청주시와 대전시가 금강 본류의 주 수질 오염원으로 나타났다. 영양 염류 (NH₃-N, NO₂-N, NO₃-N, PO₄-P)와 무기성 유해 성분 (Cl-, CN-, F-)의 함량은 지천의 일부 지점을 제외하고는 극히 낮은 수준으로 검출되었으며, 유기인, 페놀류, PCB 성분은 조사기간 중 전혀 검출되지 않았다. 또한 Fe, Zn, Cu, Mn, Cr, Pb, Cd, Hg등의 중금속은 검출되지 않거나 하천 수질 기준 또는 식용수 수질 기준의 허용치를 초과하지 않는 양호한 수준으로 나타났다.

페놀분해 효모 Candida tropicalis PW-51의 분리 및 분해특성

김성빈,김치경,김희식,이창호,신기선,권시석,윤병대,오희목 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

본 연구는 페놀과 포름알데히드를 포함하는 페놀계수지 산업폐수의 생물학적 처리에 이용할 목적으로 포름알데히드의 존재하에서 페놀분해능이 우수한 효모를 sludge로부터 분리하여, 형태적 및 생리 생화학적 특징을 조사하여 Candida tropicalis PW-51로 동정하였다. 회분식 배양에서 C. tropicalis PW-51의 페놀 분해한계는 2,000 mg/ℓ이며, 58시간내에 완전히 분해하였다. C. tropicalis PW-51은 초기접종량이 9×10 exp (6) cells/㎖, 배양온도는 30℃, pH는 7.0에서 페놀 분해효율이 높았으며, 500~2,000 mg/ℓ의 페놀농도에서 조사된 페놀에 대한 평균 분해율은 페놀 1,000 mg/ℓ에서 45.5 mg/ℓ/h 가장 높았다. C. tropicalis PW-51의 페놀분해시 catechol 1,2-dioxygenase의 활성이 크게증가하므로 ortho-path-way에 의해 페놀을 분해하는 것으로 판단되었다. 따라서, C. tropicalis PW-51는 페놀계 수지 산업폐수의 생물학적 처리에 효과적으로 이용될 수 있는 균주로 사료된다. For the biological treatment of phenolic resin wastewater containing phenol and formaldehyde, a phenol-degrading yeast was isolated from the papermill sludge, and then identified as Candida tropicalis PW-51 according to morphological, physiological and biochemical properties. The strain was able to degrade high phenol concentrations up to 2,000 mg/ℓ within 58 hours in batch cultures. Phenol-degrading efficiency by the strain was maximum at the culture conditions of a final concentration of 9×10 exp (6) cells/㎖ 30℃ and pH 7.0. The mean degradation rate of phenol was highest at 45.5 mg/ℓ/h in 1,000 mg/ℓ phenol from 500 mg/ℓ to 2,000 mg/ℓ phenol. Because the enzyme activity of catechol 1,2-dioxygenase increased in the course of degradation of phenol, it seems that this strain degrades phenol via the ortho-cleavage of benzene ring. The isolate C. tropicalis PW-51 could be effectively used for the biological treatment of phenolic resin wastewater.

Phenolic Wastewater Treatment by a Mixed Culture GE2 Immobilized on Activated Carbon

( Hee Mock Oh,Young Hwan Ku,Keuk Hyon Ahn,Gi Seok Kwon,Yung Hee Kho,Tae Ick Mheen,Byung Dae Yoon ) 한국미생물생명공학회 1996 Journal of microbiology and biotechnology Vol.6 No.2

Molecular, Physiological and Harvesting Study on the CO₂ Fixing Botryococcus braunii

Hee-Mock OH 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.4

Development of an indigenous microalga producing high lipids and a wide variety of carotenoids

Hee-Mock OH 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.10

Direct Colorimetric Assay of Microcystin Using Protein Phosphatase

Oh, Hee-Mock,Lee, Seog-June,Kim, Jee-Hwan,Park, Chan-Sun,Yoon, Byung-Dae The Korean Society for Biotechnology and Bioengine 2000 Biotechnology and Bioprocess Engineering Vol.5 No.6

A new direct colorimetric assay of microcystin in water and algal samples is proposed consisting of two procedures as follows: 1) the elimination of phosphorus in the sample and concentration of microcystin using a C(sub)18 cartridge, 2) the detection of the released phosphorus by the ascorbic acid method and determination of protein phosphatase (PP) inhibition by microcystin. The optimum amounts of phosphorylase ${\alpha}$ and PP-1 in 50 ${\mu}$L concentrated sample were 50$\mu\textrm{g}$/50${\mu}$L buffer and 1.0unit/50${\mu}$L buffer, respectively, for the best assay. The pH for the maximum activity of PP-1 was 8. The minimum detectable concentration for this method was about 0.02$\mu\textrm{g}$/L, which is sufficient to meet the proposed guideline level of 1$\mu\textrm{g}$ microcystin/L in drinking water. Consequently, it would seem that the proposed direct colorimetric assay using PP is a rapid, easy, and convenient method for the detection of microcystin in water and algal samples.

Effect of Oxalic Acid on the α-Amylase Activity of Germinating Soybean Seeds

Kwon, Oh-Yong,Youn, Hee-Zu,Oh, Hee-Mock 충남대학교 자연과학연구소 1982 忠南科學硏究誌 Vol.9 No.2

大豆子葉에 含有되고 있는 phytoglycogen 즉 amylose 와 amylopectin의 加水分解 反應에서 α- amylase의 inhibitor로 알려진 oxalic acid와 無機鹽類인 KCl, NaCl와의 antagonism 및 synergiam의 生理機作에 대한 一端을 究明하기 위하여, 各濃度別의 oxalic acid와 KCl,NaCl의 溶液에 處理된 大豆의 各 發芽段階별 α-amylase activity를 測定하여 다음과 같은 結果를 얻었다. 1 各 濃度別 KCl, NaCl 및 oxalic acid 溶液에서 大豆子葉의 α-amylase activity는 播種後 48時間에서 最大로 나타났다. 2. 10mM의 溶液으로 處理했을 때에 抑制效果가 KCl보다 NaCl 溶液에서 크게 나타났다. 이것은 곧 NaCl, KCl과 같은 無機鹽類가 α-amylase의 activity로 쓰여질 수 없음을 示唆하는 것이다. 3. 100㎍/㎖ 溶液의 oxalic acid에서 大體로 50 ㎍/㎖의 oxalic acid 溶液에서 보다 α-amylase activity에 대한 抑制效果가 크게 나타났다. 4. 50mM KCl 5, 100㎍/㎖ oxalic acid 5ml의 混合溶液에서 α-amylase activity는 36時間 以後에 100㎍/㎖ oxalic acid에서 보다 抑制效果를 크게 나타내었다. 따라서 KCl에 의하여 oxalic acid 의 抑制效果가 減少된 것으로 보아, antigonism이 나타난 것으로 推定된다. 5. 10mM NaCl 5 ㎖, 50㎍/㎖ oxalic acid 5㎖의 混合溶液에서 α-amylase의 activity는 播種後 40時間까지는 50㎍/㎖ oxalic acid에서 보다 顯著히 減少된 現象을 나타냈다. 結論的으로 發芽中인 大豆子葉에서 α-amylase activity는 oxalic acid, KCl, NaCl등의 溶液에 의하여 抑制作用을 받는 것으로, 이는 KCl, NaCl과 같은 無機鹽類가 α-amylase의 activitor가 되지 않으며, 또한 oxalic acid는 Krebe cycle의 中間代謝物質은 oxaloacetate가 negative feedback inhibition을 일으키는데 직접 關與하는 것으로 추정할 수 있다.