The Impeder of H2O2–Induced Cytotoxicity and DNA Damage by Salicornia herbacea

Bea Jin Ha,Sang Hun Lee,Eun Kyung Park,Chun Suk Nam,Geum Suk Kang 한국생명과학회 2006 한국생명과학회 심포지움 Vol.46 No.-

항균성 미강 단백질 필름 개발을 위한 Bacteriocin 생성균주의 선별 및 특성

김은정,김경미,한혜경,김영호,권기성,배동호 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

경제성과 항균력을 갖춘 미강단백질 필름을 개발하기 위한 연구의 일환으로 미강배지에서 bacteriocin을 생성할 수 있는 균주를 선별하고 그 특성을 조사하였다. 그 결과, Pseudomonas aeruginosa 9027에 대한 항균효과를 지닌 Pseudomonas aeruginosa 21025를 선별하였으며, 이 균이 생성한 bacteriocin은 쌀에 존재하는 미생물 및 토양미생물에 대하여 넓은 항균활성을 갖는 것으로 나타났다. 지금까지이용되고 있는 미강단백질 추출 조건에 대한 적합성을 알아보기 위해 bacteriocin의 특성을 조사하였다. Pseudomonas putida 21025에서 생성되는 bacteriocin은 50℃이사에서는 한 시간 후 활성이 저하되었으며, 헥산과 에탄올을 제외한 메탄올, 톨루엔, 클로로포름, 아세톤, 메틸 클로로포름등의 유기용매에서 활성이 현저하게 저하되었다. 그러나 이 bacteriocin은 pH 6.0-9.0에서 2시간 동안 안정하였고, 50℃이하에서 안정하였으며, 에탄올에 대하여 3시간동안 안정한 특성을 가지고 잇는 것으로 나타났다. 또한 부분정제한 bacteriocin의 분자량은 약 21.6kDa로 비교적 작은 분자량을 나타내었다. This study was conducted to select the bacteriocin-producing microorganism cultivated in the rice bran culture and to characterize the produced bacteriocin for the further purpose of economical and anti-bacterial rice bran protein film. Pseudomonas putida 21025 was cultivated from rice bran and identified as a producer of a bacteriocin which showed bacteriocin activity against Pseudomonas aeruginosa 9027. Bacteriocin produced by Pseudomonas putida 21025 showed a broad spectrum of activity against spoilage and soil bacteria. The activity of the bacteriocin produced by Pseudomonas putida 21025 decreased after 1 hr of staying at the temperature of 50℃ and with the presence of some organic solvents, except hexane and ethanol. However, the bacteriocin activity was stable throughout the pH ranges of 6-9 for 2 hrs, at the temperature lower than 50℃, and with the presence of ethanol for 3 hrs. The bacteriocin was partially purified by 50% ammonium sulfate precipitationn followed by subsequent dialysis. Direct detection of the partially purified bacteriocin on SDS-PAGE suggested that it had an apparent molecular mass of about 21.6 kDa.

인간중심돌봄에 대한 요양병원 간호사의 인식

장희경(Hee-kyung Chang),길초롱(Cho-rong Gil),김혜진(Hye-jin Kim),배한주(Han-ju Bea),양은옥(Eun-ok Yang),윤미림(Mi-lim Yoon),하자현(Ja-hyeon Ha) 한국산학기술학회 2018 한국산학기술학회논문지 Vol.19 No.11

본 연구의 목적은 인간중심돌봄에 대한 요양병원 간호사의 인식을 파악하는 것이다. 연구대상은 전라도와 경상도 내 위치한 요양병원 4곳에서 근무하고 있는 간호사 21명이며, 각 병원에 1개씩 포커스 그룹을 구성하여 총 4개의 포커스그룹에서 면담을 수행하였다. 자료 수집 기간은 2018년 7월 19일부터 7월 30일까지였고 질적 주제분석을 이용해 자료를 분석하였다. 연구결과 인간중심돌봄에 대한 요양병원 간호사의 인식은 6개의 주제와 11개의 하위주제로 도출되었으며, 6개의 주제는 “개별 요구 존중하기”, “끝까지 동행하기”, “남은 꿈 지지하기”, “가족과 동반자되기”, “집처럼 생활하도록 돕기”, “문화 변화시키기”로 나타났다. 간호사가 인식하는 인간중심돌봄은 노인의 선호도에 따라 개별화된 간호를 수행하고 다양한 활동프로그램을 통해 노인이 삶의 가치와 의미를 발견할 수 있도록 도와주는 것이었다. 또한 노인뿐만 아니라 이들의 가족 구성원과 협력적인 관계를 유지하고 의사결정을 공유하며 노인의 권리와 자율성이 존중받을 수 있는 물리적 환경 및 조직 문화를 형성하는 것을 인간중심돌봄이라고 인식하고 있었다. 본 연구결과를 토대로 향후 인간중심돌봄 문화 형성에 필요한 다각적 요구를 확인하고 인간중심돌봄을 기반으로 한 간호중재 프로그램을 개발하여 그 효과를 검증할 필요가 있다. This study was conducted to understand and describe Korean nurses’ perception of person-centered care for elderly patients in long-term care hospitals. Qualitative data were collected through four focus group interviews consisting of 21 nurses working in four long-term care hospitals in Jeolla and Gyeongsang provinces. Participants completed interviews from July 19 to 30, 2018. All interviews were recorded, transcribed, and analyzed by employing the thematic analysis method. Six main themes for the attributes of person-centered care for elderly patients in the long-term care hospitals were conceptualized: respecting individual needs, walking to the end, supporting hidden dreams, becoming a family partner, helping patients live like they are at home, and changing culture. Person-centered care perceived by nurses was conducted to provide individualized nursing according to elderly’s preferences and help them discover the value and meaning of life through various activity programs. Nurses also recognized person-centered care to maintain cooperative relationships with their family members and share their decision-making process, as well as to form a physical environment and organizational culture that respects the rights and autonomy of the elderly. Based on the results of this study, it is necessary to identify the diverse needs of the elderly and develop nursing intervention programs based on person-centered care.

Poster Session : PS 0736 ; Rheumatology ; Body Mass Index and Estrogen Predict Radiographic Progression in the Spine in Ankylosing Spondylitis

( Hye Min Jeong ),( Eun Kyung Bea ),( In Young Kim ),( Seul Kee Lee ),( Ji Won Hwang ),( Hyung Jin Kim ),( Jae Joon Lee ),( Eun Mi Koh ),( Hoon Suk Cha ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: This study aimed to evaluate the role of estrogen and BMI as predictors of radiographic spinal progression in patients with ankylosing spondylitis (AS). Methods: Forty-seven patients with AS were included in this study. Radiographs both at baseline and at the end of the study were scored using modifi ed the Stoke AS Spine score (mSASSS). A rate of 1 = unit/year was defi ned as progression. Serum estrogen levels were detected at specifi c times. The time-average BMI was calculated. Other clinical and laboratory characteristics were retrospectively collected. Results: The mean X-ray follow-up duration was 83. 7 ± 55. 2 months, and 25 (53. 2%) patients had progressed. A univariable analysis showed that an estrogen serum level of = 128. 78 pg/ml (OR 10. 77, 95% CI 2. 78 to 41. 75, p = 0. 001), BMI = 25 kg/m2 (OR 10. 83, 95% CI 2. 08 to 56. 51, p = 0. 005), baseline mSASSS score = 10 (OR 17. 78, 95% CI 3. 36 to 94. 18, p = 0. 001), sacroillitis grade (OR 2. 22, 95% CI 1. 38 to 3. 57, p = 0. 001) and disease duration (OR 1. 01, 95% CI 1. 00-1. 12, p = 0. 03) were associated with radiographic progression. A multivariable analysis showed that both estrogen = 128. 78 pg/ ml and BMI = 25 kg/m2 were signifi cant predictive factors for spinal radiographic progression together with baseline mSASSS = 10. The serum estrogen and BMI showed a weak positive correlation (r = 0. 295, p = 0. 044). Conclusions: Both an elevated serum estrogen level and a high BMI were associated with radiographic spinal progression in AS patients.

고관절 전치환술 환자의 퇴원교육 이행정도와 일상생활 활동정도와의 관계연구

이유미,임옥자,김신희,김미숙,배효숙,정은주,최혜린,송미라,박수경,서은혜,구현경 이화여자대학교 간호과학연구소 1992 Health & Nursing Vol.4 No.-

Total hip replacement is one orthopedic procedure currently being used for hip arthroplasty. In total hip replacement, the femoral head and acetabulum are both replaced by prostheses. Dislocation of a prosthesis because of inappropriate body positioning is one possible postoperative complication which occurs more frequently in the Korean life style setting than in the west. Hence the importance of pre-discharge education of patients following total hip replacement about appropriate positioning to present dislocation. This study examined the correlation between compliance with discharge education and hip-related activities of daily living of clients who had had total hip replacements. The purpose was to contribute to the development of pre-discharge educational protocols and eventually to help these clients conform to appropriate hip-related activities of daily living. The subjects were a convenience sample of 19 clients who had had total hip replacement surgery from 1988 to 1990 at E. University hospital in Seoul. The date were collected by an interview schedule from March 15 to April 17, 1992. The measurement tools were a pre-discharge deucation compliance score developed from the educational programs used in the hospital and the Harres' Hip Score which evaluated hip-related activities of daily living. The data were analysed by an SPSS computer program for percentage and by t -tests, ANOVA and Pearson Correlation Coefficients. There was a positive correlation between compliance with pre-discharge education and the Hip score(p=0.021). Age was related to compliance with pre -discharge education. The state preparation for discharge was related to both compliance with education and the Hip score. The results suggest that further research of the relationships between compliance with pre -discharge education programs and hip-related activities of daily living for clients having total hip replacements is warrenteci.

차광재배에 의한 찻잎 성분 및 말차 품질 변화

황정규(Jung gyu Hwang),심두보(Doobo Shim),임현정(Hyeon Jeong Im),박상기(Sang Ki Park),강은주(Eun-Ju Kang),조경환(Kyung hwan Cho),배성경(Seong Kyeong Bea),이지현(Ji-hyun Lee),김종철(Jong Cheol Kim) 한국차학회 2018 한국차학회지 Vol.24 No.2

Morphologic and physicochemical changes of shading cultivated tea (Camellia sinensis) tree leaves were investigated during 26 days with 95% shading treatment. Shading cultivation showed higher leaf area (10%) and moisture content (12%) than non-shading cultivation. The shading cultivation increased the total free amino acid (3.4-fold), theanine (3.7-fold), arginine (10.8-fold) and caffeine levels (2.0-fold) but decreased the total catechin contents, EGCG (epigallocatechin gallate) and EGC (epigallocatechin) levels of tea leaves. The SPAD value and total chlorophyll contents of the shading cultivation were increased by 1.1- and 2.1-fold, respectively. The results of a color analysis showed that lightness (L*) of non-shaded was higher than that of shading cultivated matcha. However, the greenness (a*) and yellowness (b*) of shading cultivated matcha were higher than those of non-shading cultivation. The surface color of shading cultivated matcha had higher chroma- and G-values than non-shading cultivated matcha, which represents real green color. Thus, the shading cultivation is suitable to prepare a high-quality matcha product.