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      • KCI등재
      • The Effects of ASMase Mediated Endothelial Cell Apoptosis in Multiple Hypofractionated Irradiations in CT26 Tumor Bearing Mice

        Zhu, Hong,Deng, Kai,Zhao, Ya-Qin,Wang, Xin,Shen, Ya-Li,Liu, Tai-Guo,Cui, Dan-Dan,Xu, Feng Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

        Background: To investigate the effects of ASMase mediated endothelial cell apoptosis in multiple hypofractionated irradiations in CT26 tumor bearing mice. Materials and Methods: Thirty-five CT26 tumor bearing mice were subjected to single ionizing radiation (IR) of 0, 3, 6, 9, 12, 15, 18 Gy. Eight hours after IR, the mice were sacrificed and tumor tissues were used for CD31 immunohistochemistry staining, TUNEL and CD31 double staining, ASMase activity assay. Then 6 and 12 Gy were chosen for multiple hypofractionated IR experiments according to the above results. Each time after IR, 5 mice were sacrificed and assayed as above. Results: The ASMase activities were increased significantly after a single IR of 12 Gy or higher which was accompanied with remarkable increased endothelial cell apoptosis and decreased MVD. For 6 Gy which was not high enough to trigger ASMase activation, after 2 or more times of IR, the ASMase activities were significantly increased accompanied with remarkable increased endothelial cell apoptosis and decreased MVD. While for 12 Gy, after 2 or more times of IR, the ASMase activities and endothelial cell apoptosis rates were maintained without remarkable increase; however, the MVD was significantly decreased. What's more, the cancer cell apoptosis rates were significantly increased after multiple IR for both 6 Gy and 12 Gy. Conclusions: ASMase mediated endothelial cell apoptosis may play an important role in the process of multiple hypofractionated IR for CT26 colorectal carcinoma.

      • SCIESCOPUSKCI등재

        Artificial Neural Network Based Mission Planning Mechanism for Spacecraft

        Li, Zhaoyu,Xu, Rui,Cui, Pingyuan,Zhu, Shengying The Korean Society for Aeronautical Space Sciences 2018 International Journal of Aeronautical and Space Sc Vol.19 No.1

        The ability to plan and react fast in dynamic space environments is central to intelligent behavior of spacecraft. For space and robotic applications, many planners have been used. But it is difficult to encode the domain knowledge and directly use existing techniques such as heuristic to improve the performance of the application systems. Therefore, regarding planning as an advanced control problem, this paper first proposes an autonomous mission planning and action selection mechanism through a multiple layer perceptron neural network approach to select actions in planning process and improve efficiency. To prove the availability and effectiveness, we use autonomous mission planning problems of the spacecraft, which is a sophisticated system with complex subsystems and constraints as an example. Simulation results have shown that artificial neural networks (ANNs) are usable for planning problems. Compared with the existing planning method in EUROPA, the mechanism using ANNs is more efficient and can guarantee stable performance. Therefore, the mechanism proposed in this paper is more suitable for planning problems of spacecraft that require real time and stability.

      • KCI등재

        Overexpression of the AtSTK Gene Increases Salt, PEG and ABA Tolerance in Arabidopsis

        Lei Bing,Cui-Cui Feng,Jing-Lan Li,Xiao-Xu Li,Baocun Zhao,Yin-Zhu Shen,Zhan-Jing Huang,Rong-Chao Ge 한국식물학회 2013 Journal of Plant Biology Vol.56 No.6

        AtSTK (At5g02800), which is a serine-threonineprotein kinase gene of Arabidopsis thaliana, was cloned, andits function was studied. The study found that the overexpressionof AtSTK could significantly improve the ability of A. thaliana to tolerate salt, PEG, and ABA stresses. RT-PCRanalysis revealed that the expression of the AtSTK genecould be obviously induced by salt, PEG, and ABA. Theexamination of the physiological characteristics showed thatthe overexpression of AtSTK in Arabidopsis significantlyreduced the plasma membrane permeability, significantlyincreased the proline content, and decreased the MDA content. These changes may reflect the physiological mechanismsthrough which AtSTK overexpression improves stress resistancein Arabidopsis. In addition, the overexpression of the AtSTKgene significantly antagonised the inhibitory effect of highconcentrations of exogenous ABA on Arabidopsis seedgermination. The subcellular localisation results showed thatAtSTK is located in both the cytosol and the nucleus. Theexamination of its tissue-specific expression showed thatAtSTK is expressed in various Arabidopsis tissues and isparticularly strongly expressed in the vessels. The signallingpathway analysis indicated that AtSTK might transfer thesalt stress signal in Arabidopsis through the MAPK pathway.

      • KCI등재

        Correction to: Artificial Neural Network Based Mission Planning Mechanism for Spacecraft

        Zhaoyu Li,Rui Xu,Pingyuan Cui,Shengying Zhu 한국항공우주학회 2018 International Journal of Aeronautical and Space Sc Vol.19 No.3

        The ability to plan and react fast in dynamic space environments is central to intelligent behavior of spacecraft. For space and robotic applications, many planners have been used. But it is difficult to encode the domain knowledge and directly use existing techniques such as heuristic to improve the performance of the application systems. Therefore, regarding planning as an advanced control problem, this paper first proposes an autonomous mission planning and action selection mechanism through a multiple layer perceptron neural network approach to select actions in planning process and improve efficiency. To prove the availability and effectiveness, we use autonomous mission planning problems of the spacecraft, which is a sophisticated system with complex subsystems and constraints as an example. Simulation results have shown that artificial neural networks (ANNs) are usable for planning problems. Compared with the existing planning method in EUROPA, the mechanism using ANNs is more efficient and can guarantee stable performance. Therefore, the mechanism proposed in this paper is more suitable for planning problems of spacecraft that require real time and stability.

      • KCI등재

        Artificial Neural Networks-Based Mission Planning Mechanism for Spacecraft

        Zhaoyu Li,Rui Xu,Pingyuan Cui,Shengying Zhu 한국항공우주학회 2018 International Journal of Aeronautical and Space Sc Vol.19 No.1

        The ability to plan and react fast in dynamic space environments is central to intelligent behavior of spacecraft. For space and robotic applications, many planners have been used. But it is difficult to encode the domain knowledge and directly use existing techniques such as heuristic to improve the performance of the application systems. Therefore, regarding planning as an advanced control problem, this paper first proposes an autonomous mission planning and action selection mechanism through a multiple layer perceptron neural network approach to select actions in planning process and improve efficiency. To prove the availability and effectiveness, we use autonomous mission planning problems of the spacecraft, which is a sophisticated system with complex subsystems and constraints as an example. Simulation results have shown that artificial neural networks (ANNs) are usable for planning problems. Compared with the existing planning method in EUROPA, the mechanism using ANNs is more efficient and can guarantee stable performance. Therefore, the mechanism proposed in this paper is more suitable for planning problems of spacecraft that require real time and stability.

      • KCI등재

        Performance and Spatial Succession of a Full-Scale Anaerobic Plant Treating High-Concentration Cassava Bioethanol Wastewater

        ( Gao Rui Fang ),( Xu Feng Yuan ),( Jia Jia Li ),( Xiao Fen Wang ),( Xu Cheng ),( Wan Bin Zhu ),( Zong Jun Cui ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.8

        A novel two-phase anaerobic treatment technology was developed to treat high-concentration organic cassava bioethanol wastewater. The start-up process and contribution of organics (COD, total nitrogen, and NH4+-N) removal in spatial succession of the whole process and spatial microbial diversity changing when sampling were analyzed. The results of the start-up phase showed that the organic loading rate could reach up to 10 kg COD/m3d, with the COD removal rate remaining over 90% after 25 days. The sample results indicated that the contribution of COD removal in the pre-anaerobic and anaerobic phases was 40% and 60%, respectively, with the highest efficiency of 98.5%; TN and NH4+-N had decreased to 0.05 g/l and 0.90 g/l, respectively, and the mineralization rate of total nitrogen was 94.8%, 76.56% of which was attributed to the anaerobic part. The microbial diversity changed remarkably among different sample points depending on the physiological characteristics of identified strains. Moraxellaceae, Planococcaceae, and Prevotellaceae were dominant in the pre-anaerobic phase and Bacteroidetes, Campylobacterales, Acinetobacter, Lactobacillus, Clostridium, and Bacillus for the anaerobic phase. Methanosarcinaceae and Methanosaeta were the two main phylotypes in the anaerobic reactor.

      • KCI등재

        Emulsifying properties of different soy hull pectin polysaccharides and application in mayonnaise

        Jun Li,Huaitian Cui,Xinyue Xu,Jiayi Li,Miaomiao Lu,Xijie Guan,Danshi Zhu,He Liu 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.6

        Soy hull pectic polysaccharide (SHPP) is a kindof biological macromolecule prepared from soy hull, whichhas certain thickening and gel properties. In present study,SHPP was extracted with citric acid and sodium citratefrom soybean hulls under the assistance of microwave,respectively. SHPPs were then compared with commercialpectin polysaccharide to test their emulsification ability. The emulsion prepared from SHPP extracted with sodiumcitrate has the best emulsifying effect, small particle sizeand uniform distribution. The rheological properties andparticle size distribution of mayonnaise did not changesignificantly after the addition of different SHPPs andcommercial pectin polysaccharides. However, microscopicobservations revealed that the droplets were more uniformlydistributed in the mayonnaise after the addition ofSHPP extracted with sodium citrate under the assistance ofmicrowave and commercial pectin APC141. SHPPextracted with sodium citrate may play an important role inmaintaining emulsion stability in the future.

      • SCIESCOPUSKCI등재

        The Phylotype of Thermus from the Rehai Geothermal Area, Tengchong, China

        Guo, Chunlei,Wang, Tao,Zhu, Wei,Zhang, Donghua,Cui, Xiaolong,Xu, Lihua,Peng, Qian The Microbiological Society of Korea 2003 The journal of microbiology Vol.41 No.2

        Through enrichment on two nutrient agars, 57 Thermus isolates were recovered from 15 hot spring samples taken from the Rehai geothermal area, Tengchong, China. Unique growth characteristics were observed when the strains were transferred from YIM14 medium to Thermus medium. Phylogenetic analysis showed that the 16S rDNA sequences of the isolates and clones from the Rehai geothermal area farmed a monophyletic group on the phylogenetic tree. A secondary structure comparison showed that their 16S rRNAs have unique secondary structure characteristics.

      • KCI등재

        Gastroprotective effect of cirsilineol against hydrochloric acid/ethanol-induced gastric ulcer in rats

        Guojin Gong,Rigetu Zhao,Yuhui Zhu,Jinfeng Yu,Bin Wei,Yan Xu,Zhaoxun Cui,Guoying Liang 대한생리학회-대한약리학회 2021 The Korean Journal of Physiology & Pharmacology Vol.25 No.5

        This study was designed to evaluate the gastroprotective activity of cirsilineol in hydrochloric acid (HCl)/ethanol-induced gastric ulcer model. Cirsilineol was administered at the doses of 20 and 40 mg/kg in HCl/ethanol-induced rats. The gastroprotective ability was verified by determining the ulcer score, total acidity, hemoglobin, inflammatory cytokines, lipid peroxides, and enzymatic antioxidants superoxide dismutase (SOD) and catalase (CAT) in gastric tissue and serum biochemical analysis. The results showed a favorable increase in the hemoglobin level, antioxidant enzymes (SOD and CAT), restored electrochemical balance (carbon dioxide & anion gap) while a noticeable decrease in ulcer index, total acidity, lipid peroxides, inflammatory cytokines (interleukin-1 beta [IL-1β], IL-6, and tumor necrosis factor alpha) in rats treated with the cirsilineol. The serum biochemical analysis on liver markers (alkaline phosphatases, alanine aminotransferase, and aspartate aminotransferase), kidney markers (urea, creatinine, albumin, globulin, total protein), and lipid profile (triglyceride, high-density lipoprotein, total cholesterol) were attenuated by cirsilineol treatment in rats. Histopathology showed enhanced gastric protection and preserved the integrity of gastric mucosa upon cirsilineol administration. These results ultimately suggest that cirsilineol has gastroprotective effects that prevent the development of gastric ulcer.

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