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Real-time PCR을 이용한 환경 중 물 시료의 레지오넬라 분석법 연구
이정희,이정희,박명기,김윤성,윤희정,이창희,정아용,윤미혜 한국환경보건학회 2019 한국환경보건학회지 Vol.45 No.5
Objectives: The standard method for the enumeration of environmental Legionella is culturing, which has several disadvantages, including long incubation and poor sensitivity. The purpose of this study is to demonstrate the usefulness of real-time PCR and to improve the standard method. Methods: In 200 environmental water samples, a real-time PCR and culture were conducted to detect and quantify Legionella. Using with the results of the survey, we compared the real-time PCR with the culture. Results: Each real-time PCR assay had 100% specificity and excellent sensitivity (5 GU/reaction). In the culture, 36 samples were positive and 164 samples were negative. Based on the results of the culture, real-time PCR showed a high negative predictive value of 99%, 35 samples were true positive, 105 samples were true negative, 59 samples were false positive and one sample was a false negative. Quantitative analysis of the two methods indicated a weak linear correlation (r 2 =0.29, r 2 =0.61, respectively). Conclusions: Although it is difficult to directly apply quantitative analysis results of real-time PCR in the enumeration of environmental Legionella, it can be used as a complementary means of culturing to rapidly screen negative samples and to improve the accuracy of diagnosis.
李柾熙 경북대학교 평화문제연구소 1983 평화연구 Vol.8 No.-
Ⅰ. 序 論 Ⅱ. 統獨政策 槪要 1) 兩獨의 統獨政策 發展過程 2) 獨逸問題 解決方案과 그 過程 Ⅲ. 西獨의 統獨政策 變遷 1) 아데나워時代의 政策 2) 에어하르트 時代 政策 3) 키징거 時代의 政策 4) 브란드 時代의 政策 Ⅳ. 結 論