廻腸穿孔性 疾患의 臨床的 考察

羅泳燦(Young-Chan Rah),睦敦相(Don-Sang Mok) 대한외과학회 1977 Annals of Surgical Treatment and Research Vol.19 No.4

腸結核에 의한 終末廻腸穿孔의 臨床的 考察

羅泳燦(Young Chan Rah) 대한외과학회 1978 Annals of Surgical Treatment and Research Vol.20 No.4

胃癌細胞의 植物凝集素結合에 關한 免疫酵素組織學的 硏究

羅泳燦,廉範愚,金世民 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1

To evaluate the binding reactions of various phytohemagglutinins (PHAs) on the gastric carcinoma cells, the author had undertaken an experiment using purified PHAs, such as Concanavalin A(Con-A), wheat germ agglutinin(WGA), soybean agglutinin(SBA), Dolichos biflorus agglutinin(DBA), Ulex europaeus agglutinin I (UEA I), peanut agglutinin(PNA), Ricinus communis agglutinin I (RCA I), and Pinellia ternata agglutinin(PTA). The tissue samples used were gastric tissue from subtotal gastrectomy or fiberoptic biopsy, diagnosed by pathologists as well differentiated adenocarcinoma and poorly differentiated adenocarcinoma signet-ring cell type adenocarcinoma, and dysplasia. The results obtained are as follows; 1. On the well-differentiated adenocarcinoma, all the PHAs except UEA I showed cytoplasmic granular reactions of the tumor cells and linear reactions along the intraluminal borders of the tumor glands. 2. On the poorly differentiated adenocarcinoma, RCA I and UEA I showed cytoplasmic reactions, and other PHAs showed variable reactions from positive to focal positive. 3. In signet-ring cell carcinoma, PNA, SBA, RCA I and UEA I showed weak reactions in the cytoplasm. 4. On the dysplastic cells, Con-A, WGA, PNA, RCA I and PTA showed cytoplasmic reactions and SBA, UEA I and DBA showed no reaction. With the above results, the binding of PHAs on the tumor cells of the stomach reveled variable patterns, depending on the degree of differentiation and the kinds of PHAs used.

Development of the Digestive Tract in Tiger Puffer (Takifugu rubripes)

김봉원,나오수,박창범,고환봉,강법세,최영찬,이영돈 한국발생생물학회 2003 발생과 생식 Vol.7 No.1

이 연구는 자주복의 개체발생 단계에 따른 소화관 외부형태의 변화와 소화관의 발달 양상을 조직학적으로 조사하였다. 부화 직전 수정란의 소화관은 난황낭과 배체의 척추 사이에 위치하였고, 형태는 거의 직선상 이었다. 입은 부화 후 2일째, 항문은 부화 후 4일째 열렸다. 부화 후 4일째 소화관은 식도, 전장, 중장 그리고 직장으로 구분이 되었고, 전장과 중장이 괄약근에 의해 구분되었다. 부화 후 5일째 자어는 전장과 중장 사이가 배측으로 굴곡되었고, 식도상피 Anatomical and histological observations were used to examine the morphological differentiation and development of the digestive tract during ontogenesis in tiger puffer, Taklyugu rubripes. Before hatching, the digestive tract was located between yolk sac and notochord of embryo. Newly hatched larvae had a straight tubular digestive tract. The larval mouth opened at 2 days after hatching (DAH). At 4 DAH, sphincters separated the digestive tract into the esophagus, anterior intestine, mid-intestine and rectum, and the anus was opened. At 5 DAH, the larval intestine bent between the anterior and intermediate parts and mucous cells developed in the esophageal epithelium. At this time yolk sac was absorbed completely. At 6 DAM, the digestive tract was differentiated into the U type with expansion of the anterior intestine toward the head of the larvae. At 10 DAH, the expanding anterior intestine became wider and a elliptical. At 15 DAH, the anterior intestine was divided into two portions by invagination of dorsal epithelium and the mid-intestine was convoluted dorsally. At 21 DAM, the frontal portion of the anterior intestine formed a differentiated expansion sac. At 24 DAH, the second convolution was observed in the anterior part of the posterior intestine. At 30 DAH the expansion sac was extended longitudinally and the digestive tract took on the form seen in adult fish.

Induction of In Vitro Vitellogenin Synthesis by Bisphenol, Nonylphenol and Octylphenol in Chinese Minnow (Phoxinus oxycephalus) Hepatocytes

박창범,김병호,나오수,최영찬,이영돈,백혜자,김형배,Akihiro Takemura 한국통합생물학회 2003 Animal cells and systems Vol.7 No.3

Bisphenol A (BPA), nonylphenol (NP), and 4-tert-octylphenol (OP) are known endocrine disrupting chemicals (EDCs) with estrogenic activity in fish. This study compared the effects of BPA, NP and OP on in vitro vitellogenin (VTG) synthesis in primary cultures of hepatocytes of the Chinese minnow Phoxinus oxycephalus. The VTG secreted into the culture medium was measured using enzyme-linked immunosorbent assay (ELISA), which we developed in this study using an antibody prepared from homogenates of Chinese minnow egg. VTG synthesis was induced by estradiol-17 (E2) and phenols (BPA, NP and OP) treatment. E2 at concentrations of 106 M or higher increased VTG levels significantly (P < 0.05). Exposure to 105 M BPA or 104 M NP and OP induced in vitro VTG synthesis (P < 0.01). However, 103 M BPA, NP or OP did not induce VTG synthesis. These results suggest that BPA has the highest estrogenic potential in Chinese minnow hepatocytes. Tamoxifen, an anti-estrogen, drastically blocked the production of VTG by phenols (BPA, NP and OP) suggesting that phenols (BPA, NP and OP) may act via binding to estrogen receptor (ER) in Chinese minnow hepatocytes.

삼척공장 6,7호 Kiln Line의 설비개선

김용규,정성남,나영찬,강문기 한국시멘트협회 2003 시멘트 심포지움 Vol.30 No.-

Nonylphenol이 넙치, Paralichthys olivaceus의 초기 발생에 미치는 영향

문순주,김진완,나오수,김병호,이영돈,김형배,최영찬 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1

Effects on nonylphenol (NP) treatment on early development of fertilized eggs and survival of larvae in olive flounder, Paralichthys olivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solutions of NP at nominal concentrations of 50, 100, 150 and 200 ㎍/L. In the control Ⅰ and Ⅱ (methanol-carrier control) groups, the hatchability of fertilized eggs were 90.7±7.02 and 90.0±5.29% (P< 0.05), respectively. However, treatments of NP concentrations at 50, 100, 150 and 200 ㎍/L were reduced to 78.7±4.16, 46.0±9.17, 48.0±3.46 and 33.3±11.02% (P<0.05), respectively. The time to hatching of fertilized eggs was delayed in high-dose NP treatment groups rather than control groups. Fertilized eggs of NP immersed group could not hatch normally, and also vertebra of the larvae observed as abnormal shape. Cumulative mortality of hatched larvae after 26 hr NP treatment was 38.3, 78.3, 88.3 and 100% in NP 50, 100, 150 and 200 ㎍/L treatment groups, respectively. Whereas, control Ⅰ and Ⅱ were 11.7, 163%(P<0.05). Hatched larvae showed most death at the embryonic development stage with abnormalities. These results suggest that NP treatment in fertilized eggs and larvae was inhibited as normally development for fertilized egg and growing of larvae.

Nonylphenol이 넙치, Paralichthys olivaceus의 초기 발생에 미치는 영향

문순주(Soon Ju Moon 외 6명),김진완,나오수,김병호,이영돈,김형배,최영찬 환경독성보건학회 2002 환경독성보건학회지 Vol.17 No.1

Effects on nonylphenol (NP) treatment on early development of fertilized eggs and survival of larvae in olive flounder, Paralichthys olivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solutions of NP at nominal concentrations of 50, 100, 150 and 200μg/L. In the control I and Ⅱ(methanol-carrier control) groups, the hatchability of fertilized eggs were 90.7±7.02 and 90.0±5.29%(P<0.05), respectively. However, treatments of NP concentrations at 50, 100, 150 and 200μg/L were reduced to 78.7±4.16, 46.0±9.l7, 48.0±3.46 and 33.31±11.02%(P<0.05), respectively. The time to hatching of fertilized eggs was delayed in high-dose NP treatment groups rather than control groups. Fertilized eggs of NP immersed group could not hatch normally, and also vertebra of the larvae observed as abnormal shape.<br/> Cumulative mortality of hatched larvae after 26hr NP treatment was 38.3, 78.3, 88.3 and 100% in NP 50, 100, 150 and 200μg/L treatment groups, respectively. Whereas, control I and Ⅱ were 11.7, 16.7%(P<0.05).<br/> Hatched larvae showed most death at the embryonic development stage with abnormalities. These results suggest that NP treatment in fertilized eggs and larvae was inhibited as normally development for fertilized egg and growing of larvae.