톨루엔 폭로근로자의 혈장중 마뇨산과 공기중 톨루엔과의 상관관계

황천현,이원진,장성훈,김형아,Hwang, Cheon-Hyun,Lee, Won-Jin,Chang, Soung-Hoon,Kim, Hyoung-Ah 대한예방의학회 2000 Journal of Preventive Medicine and Public Health Vol.33 No.1

Objectives : This study was undertaken to evaluate correlation between the levels of hippuric acid in blood plasma (HAP) and those of toluene concentration in the workplace air. Methods : Study subjects were composed of two groups; 21 workers who were occupationally exposed to toluene and 25 rural-area residents who were not exposed to any known occupational toluene source, as an exposed group and a reference group, respectively. Mean age and work duration of the exposed was 42 years and five years, respectively. Mean age of the reference was 42 years. To determine toluene concentrations in the workplace air, air sampling has been conducted for more than six hours using a personal sampler, and analyzed by a gas chromatography-flame ionization detector. Concentrations of hippuric acid in biological samples were determined by a high performance liquid chromatography-ultraviolet detector. Results : Geometric mean(geometric standard deviation) of HAP and hippuric acid in urine(HAU) for the exposed was 1.39(2.21) mg/L and 2.77(1.46) g/L, respectively, which were significantly different from those of the reference [HAP, 9.45(2.94); HAU, 0.37(0.45)]. Teluene concentration in the workplace air was 86.92(range: $45.18\sim151.23$)ppm. The level of HAP or HAU was significantly correlated (r=0.70 and r=0.63, respectively) with that of toluene in the workplace air. The estimated regression equation was logHAP(mg/L)=-3.60+1.93 log(toluene, ppm) or logHAU(g/L)=-0.85+0.67 log(toluene, ppm). The magnitude of correlation was further enhanced when analyzing relationship between toluene concentrations lower than 100 ppm and its corresponding HAP levels. Conclusion : Overall, plasma hippuric acid levels were well correlated with toluene concentrations in the workplace air, and a statistically significant correlation was observed for the samples with toluene concentration lower than 100 ppm.

Ethyoene Oxide 폭로 근로자의 말초 임파구 자매염색분체 교환에 관한 연구

황천현,박종태,장성훈,김대성,이원진,김청식,김영환 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

Operators of hospital sterilizers who use ethylene oxide(EtO) were studied to determine the exposure of EtO level and the frequency of sister chromatid exchanges(SCEs) from June 12 to July 20, 1997. To evaluate SCEs in the peripheral blood cells, we selected 22 workers at the central supply room of 4 university hospitals and 22 unexposed workers at the same hospitals according to match sex, age, and smoking habit and also did questionnaires. The mean air concentrations(8-hr TWA) of EtO at 4 university hospitals were less than 1 ppm. The SCE frequencies in exposed workers to EtO and controls were normally distributed. The SCE frequencies in exposed workers to EtO and controls were 6.42±0.63, 5.86±0.69, respectively and their differences were statistically significant(p=0.0093). But there were no statistically significant differences in smoking, alcohol intake, coffee drinking. Especially smokers who exposed to EtO were increased SCE statistically significant than the exposed group who did not smoke.

연 폭로 근로자들의 지질과산화 및 항산화상태에 관한 연구

이원진,황천현,김청식,장성훈,김양호,김해준,Lee, Won-Jin,Hwang, Cheon-Hyun,Kim, Cheong-Sik,Chang, Soung-Hoon,Kim, Yang-Ho,Kim, Hae-Joon 대한예방의학회 1998 Journal of Preventive Medicine and Public Health Vol.31 No.3

This study was started to find out if plasma malondialdehyde(MDA), $\alpha-tocopherol$ and erythrocyte superoxide dismutase(SOD) activity could be markers of biological activity resulting from exposed to lead in workers. Blood samples were randomly obtained from lead-exposed workers(n=29), $CO_2$, welders(n=60) and office workers(n=60). We used whole blood to analyse blood lead with atomic absorption spectrophotometer. Superoxide dismutase activity in erythrocyte was measured with spetrophotometer. MDA and $\alpha-tocopherol$ in plasma were measured with high performance liquid chromatography. Lead-exposed workers was significantly high in blood lead concentration$(29.37{\mu}g/d\ell)$ compared with welders$(6.42{\mu}g/d\ell)$ and office workers$(5.01{\mu}g/d\ell)$. The level of plasma MDA was significantly higher in the lead-exposed workers($1.87{\mu}mol/g$ cholesterol) than the welders($1.41{\mu}mol/g$ cholesterol) and office workers($1.41{\mu}mol/g$ cholesterol). Erythrocyte SOD activity in lead-exposed workers(56.80 U/g Hb) was significantly increased than those of welders(37.63 U/g Hb) and office workers(20.47 U/g Hb). The plasma $\alpha-tocopherol$ level of lead-exposed workers($4.93{\mu}g/g$ cholesterol) was statistically different from welders($4.25{\mu}g/g$ cholesterol) and office workers$4.28{\mu}g/g$ cholesterol). Neither age nor smoking was related to SOD or MDA level. Blood lead was significantly correlated with erythrocyte SOD activity(r=0.405), plasma MDA(r=0.296) and $\alpha-tocopherol$ (r=0.207). Plasma MDA was also significantly correlated with SOD (r=0.217). In multiple regression analysis, the change of MDA and SOD activity level related to the blood lead concentration. These results suggested that the increase of plasma MDA and erythrocyte SOD activity in lead-exposed workers had a close relationship with the oxidative stress induced by lead.

연폭로 근로자들에서 적혈구 Pyrimidine 5'-Nucleotidase 활성도 및 요중 N-Acetyl-β-D-Glucosaminidase 활성도와의 상관관계 연구

서홍규,이병국,황천현,장성훈,이성수,이원진,최재욱 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.2

In this study, we measured the activity of the erythrocyte pyrimidin 5'-nuclectidase(P5N) and urinary N-acetyl-β-D-glucosaminidase(NAG) from 154 workers exposed to lead and 43 workers not exposed. We analyzed the correlation of the P5N activity and NAG activity with other biololgical exposure indices of lead such as blood lead(PbB) and zinc protoporphyrin(ZPP). The measurement was performed by using high performance liquid chromatography(HPLC), spectrophotometer and atomic absorption spectrophotometer. The results are as follows : 1. The mean value of P5N activity for workers exposed to lead was 9.50±3.13 μmol uridine/hr/g Hb and 11.60±2.2 μmol uridine/hr/g Hb for workers not exposed. The P5N activity showed a normal distribution, but the other indices of lead showed logarithmic normal distributions. 2. The P5N activity and ZPP were decreased as PbB was increased. But the NAG activity had no correlation with changes of PbB. 3. The correlation coefficients of the P5N activity with other biological exposure indices of lead such as PbB, ZPP, NAG activity were -0.72, -0.55, and 0.05, respectively. We speculated that the P5N activity can be used as a reliable biological exposure index of lead but NAG activity can be used as a biological management index of lead.

용접공에서 혈중 중금속 농도와 혈장중 Malondialdehyde, α-tocopherol 및 적혈구내 Superoxide Dismutase 활성도에 관한 연구

이원진,장성훈,황천현,천병철,김해준 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.2

We investigated the association between the oxidative stress and the concentration of heavy metals in blood among welders. The study subjects were male manual CO₂welders(N=127) exposed to several heavy metals. The age distribution was from 20 to 63(GM=33.7) and the geometric mean(GM) of work duration was 4.2 years. We analyze the level of blood lead, cadmium, manganese and chromium which was related with the oxidative stress in the animal studies. Blood selenuim, plasma malondialdehyde(MDA), plasma α-tocopherol and erythrocyte superoxide dismutase(SOD) were analyzed as biological markers of the oxidative stress. The results of univariate analysis showed that the plasma MDA was significantly correlated with α-tocopherol(r=0.327) and blood selenium(r=0.313) concentration. But we could not find out any relationship between the erythrocyte SOD activities and those metal concentrations. Neither smoking nor alcohol consumption was related to the level of heavy metals and the oxidative stress biomarkers. As the results of multiple regression analysis with stepwise selection method, 1) the change of MDA level were dependent with the level of α-tocopherol and blood selenium concentration(R²=19.7), 2) the plasma α-tocopherol level was dependant with MDA and blood chromium concentration(R²=15.6), 3) in case of erythrocyte SOD activity, only the duration of work was significantly selected.

용접공에서 혈중 중금속 농도와 혈장중 Malondialdehyde, a-tocopherol 및 적혈구내 Superoxide Dismutase 활성도에 관한 연구

이원진(Won-Jin Lee),황천현(Cheon-Hyun Hwang),장성훈(Soung-Hoon Chang),천병철(Byung-Chul Chun),김해준(Hae-Joon Kim) 대한직업환경의학회 1998 대한직업환경의학회지 Vol.10 No.2

모 중장비 제조 사업장의 유해작업요인 평가에 관한 연구

장성훈,이원진,서창호,김종규,이영신,황천현,서동윤 건국대학교 의과학연구소 1996 건국의과학학술지 Vol.6 No.-

We evaluated the types and the amount of hazards in a heavy equipment factory and a plant factory to improve workers' health and to determine the priority in occupational health programs. We first inspected the working processes to find hazardous agents, measured them in each process environment, and compare the results with their TLVs(Threshold Level Value). This study was carried out from December 26, 1995 to January 30, 1996. The results are as follows: 1. In two factories, we identified the following hazardous agents: noise, dust, heavy metal fume, organic solvents, illumination, gases and ultraviolet light. 2. In the heavy equipment factory, some agents were over their TLVs in the following departments. 1) Preparation Department: dust, Pb, Mn, Fe₂O₃, NO₂ 2) Manufacture Department: noise, dust, Pb, Mn, Fe₂O₃, NO₂ 3) Assembly Department: noise 4) Technique Department: NO₂ 3. In the plant factory, some agents were over their TLVs in the following departments: 1) 1 Bay: illumination, Pb, Mn, Fe₂O₃ 2) 2 Bay: dust, illumination, Cu 3) 3 Bay: illumination 4) 4 Bay: noise, dust, Pb, Mn, Fe₂O₃, NO₂ 5) 5 Bay: noise, dust, Pb, Mn, Fe₂O₃, NO₂ 6) 6 Bay: Pb, Mn, NO₂ We concluded that the hazardous agents such as heavy metal fume, noise and noxious gas were required to be controlled at first.