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마우스 대식세포를 이용한 어성초(魚腥草) 물추출물의 항염효능연구
황인승 ( In Seung Hwang ),김영진 ( Young Jin Kim ),박윤수 ( Yun Soo Park ),김현주 ( Hyun Ju Kim ),김도훈 ( Do Hoon Kim ),박완수 ( Wan Su Park ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.4
The purpose of this study was to investigate effects of Houttuyniae Herba water extract (HC) on calcium release and production of various inflammatory mediators such as nitric oxide (NO), interferon-inducible protein (IP)-10, platelet derived growth factor (PDGF)-BB, keratinocyte-derived chemokine (KC), vascular endothelial growth factor (VEGF), interleukin (IL)-4, and IL-5 in lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages. Methods : NO production was measured by Griess reagent assay. Intracellular calcium level was measured with Fluo-4 assay. Levels of cytokines were measured by High-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology. Results : HC significantly decreased NO production for 24 hrs incubation at the concentrations of 10, 25, 50, 100, and 200 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). HC significantly decreased production of IP-10, KC, VEGF, and PDGF-BB for 24 hrs incubation at the concentrations of 50, 100, and 200 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). HC also significantly decreased intracellular calcium release for 24 hrs incubation at the concentrations of 25, 50, 100, and 200 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). But HC did not show any significant effect on production of IL-4 and IL-5 in LPS-induced RAW 264.7.Conclusions : The results suggested that HC has anti-inflammatory property related with its inhibition on the production of NO, IP-10, KC, VEGF, and PDGF-BB in LPS-induced macrophages via calcium pathway
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金鍾先,高興煥,黃寅勝,金基雄 梨花女子大學校 韓國生活科學硏究院 1988 韓國生活科學硏究院 論叢 Vol.41 No.-
On the basis of a theoretical tenet that voluntary sports commitment is a result of participation motivation both inherent in and extrinsic to the participant, a survey research was conducted with 664 university students sampled from six major universities located in the metropolitan Seoul area. It was theorized that the intrinic motivation for sports commitment is intimately associated with the relative degree of sport socialization which is postulated to be highly dependent upon personal attributes (e.g., sex, sport aptitude, interest, attitude, etc.) as well as upon influences from parents, peers, and teachers during the maturing ages, i.e., elementary and secondary school ages. A self-report sports commitment questionnaire along with a sport socialization scale assessed 1) the present levels of sports commitment of respondents and 2) the degrees of sport socialization as well as socializing factors including personal attributes and influences from significant others. Data analysis(using ANOVA, multiple regression analysis, etc.) revealed that: 1) a very low percentage of respondents practices sports and exercises; 2) even the sport-committed group is engaged mostly in a limited kind of sports learned at the schools, instead of such life-time oriented individual and dual sports as bowling, badminton and jogging, with the frequency and duration being low and short; 3) the main reasons for participating in sports are such extrinsic factors as improvement of health and weight control, rather than pursuit of fun, enjoyment, achievement and win; 4) in contrast, the main reasons for not participating in sports are lack of interest, concern, and skill as well as lack of time and facilities; 5) the sports commitment is significantly influenced by the degree of sport socialization which, in turn, is determined, to a great degree, by such socializing factors as personal interest, attitude, sex, birth place, and influences from significant others. In order to promote mass sports, thus, these results, taken together, suggested a need for more emphasis on school physical education directed toward enhancing the awareness, interest, and attitude toward sports along with the provision of space and facilities for mass sports from the governmental standpoint.
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