Methicillin resistant staphylococcus aureus에 대한 지유산의 항균활성

황해,강옥화,권동렬,Hwang, Hae,Kang, Ok-Hwa,Kwon, Dong-Yeul 한국생약학회 2022 생약학회지 Vol.53 No.2

Methicillin resistance Staphylococcus aureus (MRSA) is a gram-positive bacterium, the most commonly isolated bacterial human pathogen. JiYu-san is one of the natural products used to treat diseases in the folk recipe. In this study, we investigated the antimicrobial activity of EtOH 70% extracts of JiYu-san (JYS) against MRSA. The antibacterial activity of JYS against MRSA strain was evaluated using minimum inhibitory concentration (MIC), checkerboard dilution test, and time-kill assay. The effect of JYS on the immune mechanism of MRSA was confirmed through cell membrane permeability tests and energy metabolism tests, and the antibacterial activity mechanism was performed using qRT-PCR and western blot. As a result, in the antibacterial test of JYS, the MIC was measured to be 1.9~1000 ㎍/mL, and synergistic or showed a partial synergistic effect. In addition, JYS showed antibacterial activity in a combination test with DCCD or TX-100. In a study on the mechanism of action of antibacterial activity, it was found that JYS suppressed MRSA resistance genes and proteins. These results suggest that JYS has antibacterial activity and provides great potential as a natural antibiotic by modulating the immune mechanism against MRSA.

양돈 슬러리에 첨가된 발효탄수화물의 종류별 악취물질 농도 비교

황옥화,양승학,전중환,곽정훈,최동윤,양승봉,김두환,조성백,Hwang, Ok Hwa,Yang, Seung Hak,Jeon, Jung Hwan,Kwag, Jeong Hoon,Choi, Dong Yun,Yang, Seung Bong,Kim, Doo Hwan,Cho, Sung Back 한국축산환경학회 2013 한국축산시설환경학회지 Vol.19 No.1

Slurry treatments included peanut shell, palm golden fiber, almond hull, which was added 2% of the amount of slurry, and non-treatment control (n=4 each group). Levels of odorous compounds were measured from the liquid slurry incubated in $20^{\circ}C$ for 2 wk in chamber whose structure is similar to slurry pit. Concentration of phenols and indoles was higher (p<0.05) in control (48.4, 4.0 ppm) compared to almond hull (31.5, 1.4 ppm) or palm golden fiber (29.1, 1.6 ppm) group. Short chain fatty acid (SCFA) level was lowest (p<0.05) in control (2,121 ppm) but highest in peanut shell group (3,640 ppm). Branched chain fatty acid (BCFA) concentration was highest (p<0.05) in peanut shell (296 ppm), but lowest in almond hull (90 ppm). Taken together, concentration of odorous compounds was decreased by addition of almond hull in pig slurry by which crude fiber and non-digestible fiber (NDF) may act as a carbon source.

초지 및 농경지에 살포되는 돼지 분뇨의 냄새에 미생물 첨가 및 분뇨 배양온도가 미치는 효과

황옥화,박성권,한덕우,이상룡,곽정훈,조성백,Hwang, Ok Hwa,Park, Sung Kwon,Han, Deug Woo,Lee, Sang Ryoung,Kwag, Jeong Hoon,Cho, Sung Back 한국초지조사료학회 2016 한국초지조사료학회지 Vol.36 No.2

초지 및 농경지에 비료로 살포되는 돼지 분뇨에서 불쾌한 냄새가 발생되면 가축 분뇨의 이용 및 냄새 민원의 발생에 영향을 줄 수 있다. 본 연구는 돼지 분뇨의 냄새를 감소하기 위하여 냄새 저감용으로 많이 이용되는 미생물 3종을 분뇨에 살포한 후 봄 가을($20^{\circ}C$) 및 여름($35^{\circ}C$)에 해당되는 온도에서 2주간 배양한 다음 냄새물질의 농도를 평가하였다. 분뇨를 $20^{\circ}C$에서 배양하였을 때 인돌류와 휘발성 지방산의 농도가 광합성균 처리구에서 낮았다(p<0.05). 분뇨를 $35^{\circ}C$에서 배양하였을 때에는 냄새물질의 농도가 미생물 처리구 간에 유의적인 차이가 없었다(p>0.05). 돼지 분뇨의 배양온도가 $20^{\circ}C$에서 $35^{\circ}C$로 상승되었을 때 냄새물질의 농도가 크게 증가되었다(p<0.05). 이상의 연구결과를 종합하면, 봄 가을($20^{\circ}C$)에는 돼지 분뇨의 냄새를 저감하기 위한 냄새저감 미생물로 광합성균을 이용할 수 있고, 여름 ($35^{\circ}C$)에는 분뇨에 첨가한 미생물의 활성을 높일 수 있는 다른 물질의 개발이 필요할 것으로 판단된다. 결론적으로, 돼지 분뇨에서 발생되는 냄새를 줄이는 것은 초지 및 농경지에 살포되는 가축 분뇨의 영양학적 가치를 높이고 이용성을 향상시키기 위해서 매우 중요하다. Odor in pig manure affects the distribution of the manure over grass and crop fields as fertilizer. The objective of this study was to investigate the effect of different types of microbes (Saccharomyces cerevisiae, Bacillus subtilis and Rodobacter capsulata) and incubation temperatures ($20^{\circ}C$ and $35^{\circ}C$) on the levels of odorous compounds in pig manure. Pig manure was incubated with 0.03% microbes (v/v) at temperatures of $20^{\circ}C$ or $35^{\circ}C$. At incubation temperature of $20^{\circ}C$, the addition of Rodobacter capsulata significantly (p<0.05) decreased the levels of indoles and volatile fatty acid (VFA). At incubation temperature of $35^{\circ}C$, the addition of any microbes of the three used in this study did not significantly (p>0.05) affect the levels of odorous compounds. When incubation temperature was increased from $20^{\circ}C$ to $35^{\circ}C$, levels of odorous compounds were significantly (p<0.05) increased. Taken together, these results suggest that Rodobacter capsulata could be utilized to reduce odor from pig manure in the spring and fall when the average temperature is around $20^{\circ}C$. However, alternative odor-reducing technology is needed to be developed to apply onto pig manure during the hot summer season ($35^{\circ}C$).

양돈 슬러리에 첨가된 악취저감물질 수준별 휘발성 지방산과 휘발성 유기화합물 농도 비교

황옥화,양승학,전중환,김중곤,최동윤,조성백,Hwang, Ok-Hwa,Yang, Seung-Hak,Jeon, Jung-Hwan,Kim, Jung-Kon,Choi, Dong-Yun,Cho, Sung-Back 한국축산환경학회 2013 한국축산시설환경학회지 Vol.19 No.2

This study was to investigate the effect of addition levels of odor reducing contents on reducing the concentration of odorous compounds. Slurry treatments included three levels mixture of horseradish powder (HP), mushroom waste (MW) and probiotics powder (PP), and non-treatment control (n=4 each group). Levels of odorous compounds were measured from the liquid slurry incubated in room temperature ($20{\sim}25^{\circ}C$) for 2 wk in chamber whose structure is similar to slurry pit. Concentration of phenols and indoles was lower (p<0.05) in level 1, which was mixed HP 0.01%, MW 0.4% and PP 0.004% (98.69, 1.87 ppm) compared to control. Short chain fatty acid (SCFA) and branched chain fatty acid (BCFA) was lowest (p<0.05) level 1 (6,557, 1675 ppm). Taken together, lower level are effective in reducing odorous compounds in pig slurry.

초지환원용 비육돈의 분과 뇨에 있어서 휘발성유기물과 휘발성지방산 농도 비교

조성백,황옥화,양승학,곽정훈,최동윤,양성봉,김두환,박성권,Cho, Sung Back,Hwang, Ok Hwa,Yang, Seung Hak,Kwag, Jeong Hoon,Choi, Dong Yun,Yang, Sung Bong,Kim, Doo Hwan,Park, Sung Kwon 한국초지조사료학회 2014 한국초지조사료학회지 Vol.34 No.2

초지 또는 농경지에 살포되는 퇴비와 액비에 의해 발생될 수 있는 악취민원에 대처하기 위하여, 돼지의 분과 뇨에 존재하는 악취물질 농도를 비교 분석하였다. 1. 비육돈의 분과 뇨에 함유된 휘발성유기물의 농도 비교 페놀, p-크레졸 및 페놀류 농도는 분 보다 뇨에서 높았으며 (p<0.05), 분 보다 뇨에 축적된 비율이 페놀은 138배, p-크레졸은 545배 높았다. 인돌 농도는 분 보다 뇨에서 높았으며, 스카톨은 뇨 보다 분에서 높았다 (p<0.05). 휘발성유기물은 대부분 뇨로 배설되었으며, 이 중 농도 및 악취강도가 가장 높은 물질인 p-크레졸이 뇨에 많이 함유되어 있다. 돼지 분뇨를 고액분리 하였을 때 퇴비로 이용되는 고체보다 액비로 이용되는 액상물질에서 악취가 훨씬 강할 것으로 예측된다. 2. 비육돈의 분과 뇨에 함유된 휘발성지방산의 농도 비교 아세트산과 단쇄지방산 농도는 분 보다 뇨에서 높았으며 (p<0.05), 부티르산과 프로피온산은 차이가 없었다 (p>0.05). 이성체지방산 중 I-부티르산의 농도는 뇨 보다 분에서 높았지만 (p<0.05), I-발레르산과 이성체지방산은 차이가 없었다 (p>0.05). 휘발성지방산 중에서 아세트산과 프로피온산은 분에서 각각 65와 20%, 뇨에서는 각각 93과 4%로 휘발성지방산의 대부분을 차지하였지만 아세트산과 프로피온산을 포함하는 단쇄지방산은 악취강도가 낮다. 반면에 악취강도가 상대적으로 높은 이성체지방산은 분과 뇨 간에 차이가 없기 때문에 휘발성지방산이 악취강도에 미치는 영향은 크지 않을 것으로 판단된다. 이상의 연구결과를 종합하면, 돼지 분뇨의 악취물질 중 악취강도가 높은 p-크레졸은 뇨에 많이 함유되어 있기 때문에 퇴비와 액비 생산과정 중 액상처리 시 악취강도가 매우 높을 것으로 추정된다. 다만, 악취강도는 휘발성지방산의 농도 변화에 따라 차이가 있을 것으로 생각된다. This study was performed to compare the level of odorous compounds in feces and urine of finishing pigs. Feces and urine from 16 finishing pigs were separately collected for 28-d. Concentrations of volatile organic compound (VOC; phenols and indoles) and volatile fatty acid (VFA; SCFA and BCFA) were measured in feces and urine. Amount of phenols and p-cresol was higher (P<0.05) in urine than in feces. Urinal levels of phenols and p-cresol were 257.8 ppm and 250.9 ppm, and those of fecal phenols and p-cresol were 0.50 ppm and 0.05 ppm, respectively. There was no difference in concentration of indoles from feces (1.0 ppm) and urine (1.8 ppm). Short chain fatty acid (SCFA) level in urine was higher (P<0.05) than in feces showing 4,547 ppm and 863 ppm, respectively. Proportion of acetic acid to total SCFA was higher in urine (94%) than in feces (66%). However, level of branched fatty acid (BCFA) was greater in feces (118 ppm) compare to that of urine (87 ppm). Odorous compounds analyzed in the current study, phenols and SCFA, were contained more in urine than in feces. Greater amount of VFA is typically found in feces than in urine since it is generated in the large intestine. However, urine contained more VFA than feces in the current study. Therefore, it will be necessary to exploit odor reducing techniques especially for pig urine as grassland fertilizer.

슬러리 환경개선물질이 초지환원용 양돈 슬러리의 악취물질 농도에 미치는 효과

조성백,황옥화,이준엽,김중곤,최동윤,박성권,Cho, Sung Back,Hwang, Ok Hwa,Lee, Jun Yeop,Kim, Jong Kon,Choi, Dong Yoon,Park, Sung Kwon 한국초지조사료학회 2013 한국초지조사료학회지 Vol.33 No.4

본 연구는 양돈 슬러리의 악취물질 감소에 효과가 있는 것으로 확인된 물질의 효능을 비교분석하기 위하여 수행되었다. 페놀류 농도는 고추냉이와 아몬드피 구에서 낮았고, 인돌류 농도는 아몬드피 구에서 가장 낮았다(p<0.05). 따라서 슬러리에 아몬드피를 첨가하면 VOC 농도가 감소되었다. SCFA 농도는 팽이버섯폐배지와 아몬드피 구에서 높았고, BCFA 농도는 대조구에 비해 다른 모든 처리구에서 낮았다(p<0.05). 또한 슬러리의 pH는 팽이버섯폐배지와 아몬드피 구에서 낮았다(p<0.05). 따라서 팽이버섯폐배지 또는 아몬드피를 슬러리에 첨가하면 SCFA 농도는 증가되고 BCFA 농도는 감소되는 것으로 나타났다. This study investigated the effect of slurry additives on reducing the concentration of odorous compounds. Slurry treatments included control, horseradish, spent mushroom, and almond hull (n = 4 each group). Odorous compounds including volatile organic compounds (VOCs phenols and indoles) and volatile fatty acids (VFAs short chain FA, SCFA, and branched chain FA, BCFA) were analyzed. Pigs of 85~120 kg body weight were fed diets formulated to meet the Korean Feeding Standard (2012) and their excretion was sampled from slurry pits. VOCs decreased (p<0.05) in horseradish, spent mushroom, and almond hull, compared to the control. SCFA was lower (p<0.05) in the horseradish group and BCFA was lower (p<0.05) in all three treatment groups compared to the control. Taken together, the tested additives are effective in reducing odorous compounds in pig slurry, indicating that the use of spent mushroom and almond hull can not only recycle the agri-byproduct but also reduce the impact of odors on surrounding communities.

발효탄수화물 첨가 비육돈사료가 초지환원용 슬러리의 악취물질 농도에 미치는 영향

조성백,황옥화,박규현,최동윤,양승봉,김두환,박성권,Cho, Sung Back,Hwang, Ok Hwa,Park, Kyu Hyeun,Choi, Dong Yun,Yang, Seung Bong,Kim, Do Hwan,Park, Sung Kwon 한국초지조사료학회 2013 한국초지조사료학회지 Vol.33 No.4

양돈 사료에 발효탄수화물을 첨가하여 돼지의 슬러리에서 악취물질의 농도를 평가하였다. VFA 분석결과, 분뇨의 SCFA 농도는 대조구, 땅콩껍질, 아몬드피 및 골든화이버구에서 각각 1,893, 1,591, 1,433 및 1,319 ppm으로 대조구에서 가장 높았고(p<0.05), 아몬드피와 골든파이버 구에서 가장 낮았다(p<0.05). VFA의 구성을 살펴보면 SCFA 중에서 아세트산의 비율이 가장 높으며, 다음으로 프로피온산, 부티르산, BCFA 순으로 낮았다. BCFA의 농도는 대조구, 땅콩껍질, 아몬드피 및 골든화이버 구에서 각각 98, 92, 78 및 74 ppm으로 대조구에서 가장 높았고(p<0.05), 골든화이버 구에서 가장 낮았다(p<0.05). VOC 분석결과, 분뇨의 페놀류 농도는 대조구, 땅콩껍질, 아몬드피 및 골든화이버 구에서 각각 97.3, 47.0, 54.3 및 33.3 ppm으로 골든화이버 구에서 가장 낮았으며, 대조구에서 가장 높았다(p<0.05). 그리고 p-크레졸 농도가 페놀류 농도의 93~96%를 차지하였다. 인돌류 농도는 대조구, 땅콩껍질, 아몬드피 및 골든화이버 구에서 각각 1.8, 1.3, 1.2 및 1.0 ppm으로 발효탄수화물 처리구간에 차이가 없었으며(p>0.05), 대조구에서 가장 높았다(p<0.05). ${NH_4}^+$-N의 농도는 대조구, 땅콩껍질, 아몬드피 및 골든화이버 구에서 각각 1,395, 995, 995 및 836 ppm으로 대조구에서 가장 높았으며, 골든화이버 구에서 가장 낮았다(p<0.05). 가축의 장내 또는 슬러리에서 발효과정 동안 미생물이 성장을 위해 필요로 하는 에너지가 제한요소로 작용되면, 미생물은 단백질을 에너지원으로 이용하기 때문에 많은 아미노산이 분해되어 악취물질이 증가 될 수 있다. 따라서 다른 처리구에 비하여 골든화이버 구에서 VOC의 농도가 가장 낮은 것은 골든화이버의 높은 NDF 함량으로(Getachew et al., 2004) 단백질 발효보다 탄수화물 발효가 활발하게 일어나 악취물질의 농도가 낮았다고 판단된다. This study investigates the effect of fermentable carbohydrate on the concentration of odorous compounds in pig slurry. Four types of pig diet were studied: control, peanut hull (crude fiber 29.87, NDF 44.02%), golden fiber (crude fiber 48.77, NDF 65.88%), and almond hull (crude fiber 44.30, NDF 64.44%). Pigs (average BW 37.0 kg) were fed diets that met the Korean Feeding Standard (2012) and their excreta samples were collected from the slurry pits. Levels of volatile organic compounds (phenols and indoles) and volatile fatty acids were analyzed by gas chromatography. Phenol level was the lowest (p<0.05) in golden fiber (33.26 ppm) group and the highest in control (97.29 ppm). The concentration of indoles in the peanut hull (1.27 ppm), almond hull (1.20 ppm), and golden fiber (1.02 ppm) groups was lower (p < 0.05) than that of control (1.79 ppm). Levels of short chain fatty acid (SCFA) were lower (p < 0.05) in golden fiber (1,319 ppm) and almond hull (1,433 ppm) groups than in control (1,893 ppm). Concentration of branched chain fatty acid (BCFA) in the golden fiber group (74 ppm) was lower (p < 0.05) than that of control (98 ppm). Taken together, the concentration levels of phenols, indoles, and VFAs decreased on addition of peanut hull, golden fiber or almond hull to the diet, suggesting that fermentable carbohydrate may contribute to reducing odorous compounds in pig slurry.

액비화 과정 중 인 이용 우수미생물 분리 및 특성

임정수,조성백,황옥화,양승학,Lim, Joung-Soo,Cho, Sung-Back,Hwang, Ok-Hwa,Yang, Seung-Hak 한국축산환경학회 2014 한국축산시설환경학회지 Vol.20 No.2

This study was conducted to investigate the bacterial capability to accumulate phosphorus during liquid composting process of pig slurry. Storage liquid compost and pig slurry were analyzed by using MALDI-TOF technique, which showed the colonies of Acinetobacter towneri and Bacillus licheniformis. In addition, bacterial colonies were isolated under high phosphoric acid conditions using X-phosphate MOPS medium with the addition of 2 mM $K_2HPO_4$. Microbial growth was observed in high and low phosphoric conditions due to the growth of bacterial diversities in the liquid fertilizer and slurry. The colonies isolated in the high phosphoric acid medium were uncultured bacterium clone and Acinetobacter sp. were identified by analysis of 16S rRNA gene sequences. Uncultured bacterium showed higher growth rate and excellent phosphorus ability then Acinetobacter sp.. In addition to Paenibacillus sp. AEY-1 isolated from pig slurry performed excellent phosphorus utilizing capability.

Vitek 2 Compact System을 이용한 Bacillus licheniformis의 동정 및 NH₃ 저감효과

임정수,한덕우,이상룡,황옥화,곽정훈,조성백,Lim, Joung-Soo,Han, Deug-Woo,Lee, Sang-Ryong,Hwang, Ok-Hwa,Kwag, Jung-Hoon,Cho, Sung-Back 한국축산환경학회 2015 한국축산시설환경학회지 Vol.21 No.3

An attempt to produce more pigs in limited spaces inevitably generalized concentrated feeding operation (CFO). As concentrated pig production practice expanded, concerns on environmental issues grow concurrently. Since odor is the concerned most among those, we attempted to develop means to tackle odor emission from livestock operations. Previously, we excavated few microorganisms from pig manure and, one of them, Bacillus licheniformis was particularly useful to handle odor problem. In this study, we conducted our investigation to further characterize Bacillus licheniformis. Strain identification was conducted using Vitek 2 compact, and the optimal temperature and pH conditions to growth B. licheniformis were searched for by analyzing turbidity on O.D 600 nm. Results of this study can be summarized as these, (1) it was re-verified that the bacterial strain that purified from pig manure was, in fact, Bacillus licheniformis, (2) the bacterial growth was highest when the temperature was kept at $30^{\circ}C$, also (3) growth rate was dependent on media pH as it was high at neutral (6, 7 and 8) but dropped when it was diverged from neutral (4, 5, 9 and 10), and (4) regarding ammonia removal efficiency, B. licheniformis recorded 64% effectiveness after 48 h incubation and reached its highest (80%) at 72 h.

폭염시 착유우 고온스트레스 경감을 위한 빗물관수시스템 이용 효과

최동윤,조성백,박규현,양승학,황옥화,곽정훈,정광화,송준익,유용희,안희권,Choi, Dong-Yoon,Cho, Sung-Back,Park, Kyu-Hyun,Yang, Seong-Hak,Hwang, Ok-Hwa,Kwag, Jung-Hoon,Jeong, Kwang-Hwa,Song, Jun-Ik,Yoo, Yong-Hee,Ahn, Hee-Kwon 한국축산환경학회 2012 축산시설환경학회지 Vol.18 No.suppl.

본 연구는 우리나라 여름철 고온기, 특히 폭염 시에 젖소의 생산성을 유지시켜 주기 위한 방법으로 빗물저장시스템을 이용하여 착유우사 지붕에 저장빗물 관수를 실시했을 때와 실시하지 않았을 때의 우사내외 온도, 착유우 산유량, 호흡수 및 직장온도 등의 변화를 측정하여 비교하였다. 1. 사료섭취량은 옥수수사일리지의 경우, 우사지붕에 관수를 하지 않았을 때는 29.7 kg을 섭취한 반면에 관수를 했을 때는 34.9 kg을 섭취했으며, 농후사료는 각각 9.2, 10.2 kg을 섭취하였다. 2. 우사지붕에 관수를 했을 때와 관수를 하지 않았을 때의 온도를 상호비교를 해 보면, 우사지붕의 온도는 관수를 했을 때는 관수를 하지 않았을 때보다 $3.6^{\circ}C$가 낮았고, 우사 내부의 온도도 $4.4^{\circ}C$가 낮았다. 3. 시험기간 중 두당 일평균 산유량을 보면 우사지붕에 관수를 하지 않았을 때는 24.5 kg으로 조사되었고, 관수를 했을 때는 27.4 kg으로 조사되어 관수를 실시했을 경우, 관수를 하지 않았을 때보다 11.8%의 산유량이 증가하는 효과를 보였다. 4. 착유우 호흡수는 우사지붕에 관수를 하지 않았을 때는 114.0회/분으로 조사되었고, 관수를 했을 때는 96.7회/분으로 조사되었으며, 관수를 실시했을 경우, 관수를 하지 않았을 때보다 호흡수가 감소하는 결과를 보였다. 5. 착유우의 직장온도는 우사지붕에 관수를 하지 않았을 때는 $40.6^{\circ}C$로 조사되었으나, 관수를 했을 때는 $39.8^{\circ}C$로 조사되어 관수를 하지 않았을 때보다 직장온도가 $0.8^{\circ}C$ 감소하는 결과를 보였다. 이상의 결과를 종합하면, 여름철 폭염기에 착유우의 고온스트레스를 경감시켜 착유우의 생산성을 지속시키기 위해서는 빗물저장시스템을 이용하여 우사 지붕을 관수하는 것이 효과적인 것으로 나타났다. Since the temperature during summer is higher than the temperature which is suitable for milking cows, high temperature stress have severely affected dairy farmers due to decreased milk production. This study was carried out to investigate the effect of rainwater sprinkling system for reducing heat stress in milking cows during intense heat time. Treatment conditions were divided into 2 types ; No rainwater sprinkling (T1) and Rainwater sprinkling (T2). Temperature in the milking cow shed, feed intake, milk yield, respiration rate and rectal temperature were measured to investigate the effect of hot environment on the physiological, productive responses in milkig cows. The results obtained are summarized as follows : The daily milk yields were 24.5 and 27.4 kg per head in T1 and T2 during experimental period. The respiration rate was significantly higher in T1 than those of T2. The rectal temperature in T1 ($40.6^{\circ}C$) was higher than those of T2 ($39.8^{\circ}C$). It was suggested that rainwater sprinkling system installed in the dairy cow shed was effective means to reduce heat stress of milking cow.