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Aniline 분해세균 Delftia sp. JK-2에서 분리된 Catechol 2,3-dioxygenase의 N-말단 아미노산 서열 분석
황선영,강형일,오계헌,Hwang Seon-Young,Kahng Hyung-Yeel,Oh Kye-Heon 한국미생물학회 2005 미생물학회지 Vol.41 No.1
본 연구에서는 이 전 연구에서 단일 탄소원과 질소원 및 에너지원으로 aniline을 이용하는 Delftia sp. JK-2에서 분리, 정제된 바 있는 C2,3O의 N-말단 아미노산과 DNA 서열을 분석하였다. Aniline에서 배양한 Delftia sp. JK-2에서 분리된 약 35kDa의 C2,3O의 N-말단 아미노산 서열을 분석 한 결과 $^1MGVMRIGHASLKVMDMDAAVRHYENV^{26}$로 Pseudomonas sp. AW-2와 Comamonas sp. JS765의 C2,3O와 일치하는 것으로 나타났다. 위에서 확인된 아미노산 서열을 바탕으로 제작된 primer와 JK-2의 total genomic DNA를 기질로 사용하여 PCR을 수행한 결과 약 950 bp의 유전자 증폭산물을 획득하였다. 이 증폭산물 중 정확히 확인된 890 bp의 염기서열을 분석한 결과 Delftia JK-2의 C2,3O유전자 염기서열은 Pseudomonas su. AW-2의 C2,3O와 일치하였으며 Comamonas sp. Js765의 C2,3O와 $97\%$의 높은 상동성을 나타내었다. The aim of this work was to investigate the N-terminal amino acid sequence of catechol 2,3-dioxygenase isolated from Delftia sp. JK-2, which could utilize aniline as sole carbon, nitrogen and energy source. Molecular weight of the enzyme was determined to approximately 35 kDa by SDS-PAGE. N-terminal amino acid sequence of C2,3O from strain JK-2 was $^1MGVMRIGHASLKVMDMDAAVRHYENV^{26}$, and exhibited high sequence similarity with that of C2,3O from Pseudomonas sp., Comamonas sp. JS765, Comamonas test-osteroni, or Burkholderia sp. RP007. Approximately 950-bp C2,3O was obtained through PCR using the primers derived from N-terminal amino acid sequence. Analysis of the DNA sequence revealed that the deduced 296 amino acid sequences were determined, and it showed $100\%$ identity with C2,3O from Pseudomonas sp. AW-2 and $97\%$ similarity with Comamonas sp. JS765.
EU의 무역구제조치: 커미톨로지(특별의사결정절차)에 대한 연구
황선영(Seon-Young Hwang) 한국관세학회 2020 관세학회지 Vol.21 No.4
As the final decision authority was transferred from the Council of the European Union to the European Commission in the decision process for the trade defence measures, an important decision was made to signal the beginning of another aspect of the new trade policy. Can do It has been predicted that rational legal judgments will be made based on economic facts according to these procedures. I saw that there is a need, and the purpose of this paper is to analyze the decision-making system based on this need. To this end, I selected a methodology that analyzes the specific decision process by looking at the cases that caused the greatest impact among the decisions made through the comitology. As a result, a fair result of the transfer of authority could not be expected, and a report by the European Enterprises Monitoring Group in 2019 found that this conclusion was valid. Accordingly, in the case of Korean companies subject to trade relief measures, when they go to a lawsuit, they can strongly assert procedural problems in the decision to take defence measures, and the courts are expected to hear such arguments as there are previous case law. Therefore, it is necessary to clarify in advance that there is no problem in actual content if it is subject to trade relief measures, and if you want to avoid being subject to the target, it seems that it will be helpful to use a local Brussels consultant.
황선영(Seon Young Hwang),노현탁(Hyun Tak Roh) 한국경영학회 2013 經營學硏究 Vol.42 No.6
This study attempts to clarify how racial diversity influences organizational turnover rates. By investigating the possibility of asymmetric responses of whites (majority) versus racial minorities, the authors suggest that status differentials between majority and minority groups may lead each group to pursue different identity-enhancing strategies, which in turn can result in different responses toward racial diversity in an organization. Moreover, this study examines non-linear effects of racial diversity especially on minority members considering their power characteristics within an organization. Two hypotheses were suggested: while the relationship between racial diversity and minority groups` turnover rate takes an inverted U-shaped form (Hypothesis 1), the association between racial diversity and majority turnover rate will be positive linear (Hypothesis 2). To test the hypotheses, a large archival data set, the Integrated Post-secondary Education Data System (IPEDS), which is collected and managed by the U.S. National Center for Educational Statistics (NCES), was utilized. The authors used and analyzed 8-year longitudinal data (2001-2004, 2007-2010) from 430 U.S. universities offering full-time undergraduate degrees (4-year basis). An independent variable (a cohort`s racial diversity) was measured during 2001-2004 and dependent measures (majority and minority groups` voluntary turnover rates) were collected during 2007-2010 with 6-year time lags. Several important controls that can influence students` turnover rates (e.g., SAT score, tuition, community characteristics) were also considered and included in the models. To analyze the data set, a panel data analysis (random effects model) was adopted. Empirical findings supported the predictions. As diversity increases, turnover rates of minority students also increase; however, as expected, after a certain level of diversity in cohorts (Blau`s index of .43 from the analysis) the pattern becomes negative (an inverted U-shaped form; support for Hypothesis 1). On the contrary, a majority`s turnover continues to increase as racial diversity increases (support for Hypothesis 2). Theoretical and empirical implications of these findings were discussed. Practical implications for Korean organizations as well as limitations of the study were also provided and discussed.