소아 원형탈모증 치험 1례

황보민,정민정,서형식,HwangBo, Min,Jeong, Min-Jeong,Seo, Hyung-Sik 대한한방소아과학회 2012 대한한방소아과학회지 Vol.26 No.2

Objectives The purpose of this study is to know the effect of Baekhogainsam-Tang on alopecia areata. Methods We decided the treatment principle through abdominal examinations and symptoms. We used herbal medicine (Baekhogainsam-Tang) to increase the roots of hair and hair. Results After the treatment, patients' hair condition had been improved (Hair loss patch diameter decreased from 2.5 cm to 0.5 cm). In addition, patient's physical symptoms were improved. Conclusions This case shows that herbal medicine, which focused on patient's physical symptom, is seemed to be effective in alopecia areata.

알레르기 비염환자의 이침(耳鍼) 치료 임상보고(臨床報告)

황보민,정민정,임정화,설재욱,양기영,서형식,HwangBo, Min,Jeong, Min-Jeong,Lim, Jung-Hwa,Sul, Jae-Uk,Yang, Gi-Young,Seo, Hyung-Sik 대한한방안이비인후피부과학회 2011 한방안이비인후피부과학회지 Vol.24 No.1

Objective : The efficacy of auricular acupuncture for the treatment of allergic rhinitis has been reported. Methods : Twelve rhinitis patients patients were enrolled in this study. Every patient received auricular acupuncture 2 times a week during 4 weeks. Auricular acupuncture points used were M101(肺), M13(副腎), M16(內鼻), M22(內分泌), M33(額), M55(神門). Patients nasal symptoms and QOL were assessed before and after 4 weeks treatment by total nasal symptom score(TNSS), total non nasal symptom score(TNNSS) and Rhinoconjuctivitis quality of life questionnaires(RQLQ). Results : 1. Among items of TNSS, nasal congestion-sneezing were significantly improved. and Total TNSS Scores were significantly improved.(p<0.05) 2. TNNSS were no significant difference. 3. Among items of RQLQ, nasal&eye problem&nasal symptom were significantly improved.

알레르기 비염환자에 대한 이침 치료 증례보고

황보민,정민정,임정화,양기영,서형식,HwangBo, Min,Jeong, Min-Jeong,Lim, Jung-Hwa,Yang, Gi-Young,Seo, Hyung-Sik 대한한방안이비인후피부과학회 2010 한방안이비인후피부과학회지 Vol.23 No.3

Objective : The purpose of this study was investigated the effect of auricular Acupuncture treatment in Allergic rhinitis patients. Methods : This study was carried out on the 16 allergic rhinitis patients who had been treated at Cheongdogun public health center for The Good-Breath program. The Good-Breath program included auricular acupuncture was performed 2 times a week during 4 weeks. Auricular acupuncture points used were M101(肺), M13(副腎), M16(內鼻), M22(內分泌), M33(額), M55(神門). Patients nasal symptoms and QOL were assessed before and after 4 weeks treatment by the Severity Scoring Systems of Rhinitis(SSSR) and Quality of Life Questionnaire for Korean Rhinitisian(QLQKR). Results : 1. Total Severity Scoring Systems of Rhinitis(SSSR) Scores were significantly improved. but Each items was no significant difference. 2. Among items of QLQKR, Itching worry about western medicine worry about change of environment Role limitation-physical were significantly improved. and Total Quality of Life Questionnaire for Korean Rhinitisian(QLQKR) Scores were significantly improved.

석곡(石斛), 석류(石榴)의 항산화, 항염증, 주름, 미백에 미치는 영향

황보민,노석선,서형식,HwangBo, Min,Roh, Seok-Sun,Seo, Hyeong-Sik 대한한방안이비인후피부과학회 2010 한방안이비인후피부과학회지 Vol.23 No.3

Objective : The aim of this study is to determine the effects of Dendrobii herba extract and Punica granatum extract on skin disease and skin beauty. Methods : To investigate in vitro anti-oxidant activity assay, ethanol extracts of medicinal plants tested by DPPH radical, xanthine oxidase activity. In the next experiment, to investigate anti-inflammatory activity assay, examined by relations in NO synthesis, IL-$1{\beta}$, IL-6, TNF-${\alpha}$, NF-${\kappa}B$, COX-2, MAP kinase. To study Skin wrinkle formation effect, we were examined by tyrosinase activities, melanin synthesis in MNT-1 cell. Results : 1. In an anti-oxidant test, Dendrobii and Punica granatum extract showed high radical scavenging activity. 2. In an anti-inflammatory test, Dendrobii herba and Punica granatum extract weakly inhibited the lipopolysaccharide(LPS)-induced nitric oxide(NO) release from RAW 246.7 macrophage cells. Dendrobii herba and Punica granatum extract also inhibited LPS-induced IL-$1{\beta}$ and COX-2 expressions. The inhibitory effect of Dendrobii herba and Punica granatum extract on macrophage activation were via the inhibition of NF-${\kappa}B$, evidenced by transient transfection assay. however, Dendrobii herba and Punica granatum extract did not have any effects about activation of Jun-N-terminal kinase(JNK) and inhibition of p38 MAP kinase in RAW 264.7 cells. 3. In the skin wrinkle formation assay, Dendrobii herba and Punica granatum extract weakly inhibited collagenase and elastase, however it was not statistically significant. 4. In the skin whitening assay, Dendrobii herba and Punica granatum extract weakly inhibited tyrosinase activity, however, it was not statistically significant. They did not have any effect on melanin synthesis, indicating that they could not be applicable for skin whitening. Conclusion : Dendrobii herba extract and Punica granatum extract may play a significant role in skin disease and skin beauty.

토사자(?絲子)의 추출용매에 따른 항염, 항산화 및 항균 효과에 대한 비교 연구

황보민,서형식,HwangBo, Min,Seo, Hyung-Sik 대한약침학회 2011 Journal of pharmacopuncture Vol.14 No.1

Objective : This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Cuscutae Semen(CS) extracted with two kinds of solvents, ethanol and distilled water. Methods : Two kinds of CS extractions were prepared 20, 50, 100 ${\mu}l/mg$. The cytotoxicity was measured by MTT assay in Raw 264.7 cell. The anti-inflammation effect was measured by inhibitory efficacy of NO Production in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes. Results : 1. Two kinds(100 ${\mu}l/mg$) of CS extraction groups had 50% cytotoxicity in Raw 264.7 cell. 2. All of CS extraction groups were not showed significantly inhibitory effect on NO production. 3. All of CS extracted with ethanol only showed dose-dependently significantly scavenging effect of DPPH radicals. 4. Two kinds of CS extractions did not have a inhibitory effect on Propionibactrium acnes. Conclusion : Two kinds(100 ${\mu}l/mg$) of CS extraction groups have 50% cytotoxicity. Two kinds of CS extractions have not the inhibitory effect on NO production and Propionibactrium acnes. CS groups extracted with ethanol only have a significantly scavenging ability of DPPH radicals. This study suggests that CS extracted with ethanol was effective in anti-oxidation.

고본(藁本)의 추출용매에 따른 항염, 항산화 및 항균 효과에 대한 비교 연구

황보민,서형식,HwangBo, Min,Seo, Hyung-Sik 대한약침학회 2011 Journal of pharmacopuncture Vol.14 No.1

Objective : This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Ligustici Rhizoma (LR) extracted with two kinds of solvents, ethanol and distilled water. Methods : It is prepared two kinds of LR extracts 20, 50, 100 ${\mu}l/mg$ by first. MTT assay way to measure cytotoxicity is formed in Raw 264.7 cell. The anti-inflammation effect is measured by ability to inhibit production of NO in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes. Results : 1. LR (20 ${\mu}l/mg$) extracted with ethanol was showed 80% cytotoxicity, LR (50 ${\mu}l/mg$) extracted with ethanol and LR (20, 50 ${\mu}l/mg$) extracted with water were showed 70% cytotoxicity, LR (100 ${\mu}l/mg$) extracted with ethanol and LR (100 ${\mu}l/mg$) extracted with water were showed 60% cytotoxicity in Raw 264.7 cell. 2. LR (100 ${\mu}l/mg$) extracted with ethanol was showed more significantly inhibitory effect on NO production than the water extraction. 3. Two kinds of LR extraction groups did not show significantly scavenging effect of DPPH radicals. 4. Two kinds of LR extractions did not have a inhibitory effect on Propionibactrium acnes. Conclusion : Two kinds of LR extracts have not cytotoxicity, statistically significant ability to scavenge DPPH radicals and effect to inhibit Propionibactrium acnes. LR extracted with ethanol only have a little effect to inhibit NO production. This study proposes that LR extracted with ethanol is more effective in anti-inflammation.

Polymerization Shrinkage and Mass Change of Dental Restorative Materials

Min Hwangbo(황보민),Ri-Mo Ku(구이모),Tae-Sung Jeong(정태성),Shin Kim(김신),Hyung-Il Kim(김형일),Yong-Hoon Kwon(권용훈) 대한치과재료학회 2012 대한치과재료학회지 Vol.39 No.2

알레르기성 비염 동물모델에서 한약제재 경구투여에 대한 국내 연구보고 고찰

조형래,황보민,Jo, Hyung-Rae,Hwangbo, Min 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.1

Objective : This study is to review the effect of herbal medicines on treatment of Allergic Rhinitis in animal models reported in Korean domestic journals.Methods : The Databases(NDSL, Koreantk, OASIS, KISS) are searched with terms as Allergic Rhinitis. among them, only animal study reports were reviewed. Animal model, intervention, and observation methods of measuring indicators were extracted.Results : 27 articles were reviewed. All studies used OVA to induce Allergic Rhinitis. 7 studies used simple herbal medicines, 20 studies used complex herbal medicines, To evaluate the effect of the Herbal medicines, 23 studies used immunological examination, 18 studies used histological examination, 16 studies used hematologic examination, 3 studies used macroscopy The evidences of 5 studies is insufficient to show that Herbal medicines are effective for treatment of Allergic Rhinitis.

안구건조증환자 11례의 한·양방 병용치료에 대한 임상보고

이동진,황보민,서형식,김수진,이종수,Lee, Dong-Jin,HwangBo, Min,Seo, Hyung-Sik,Kim, Soo-Jin,Lee, Jong-Soo 대한한방안이비인후피부과학회 2012 한방안이비인후피부과학회지 Vol.25 No.2

Objective : The endpoint of this trial is to verify therapeutical availability of conventional western eyedrops combined with acupuncture treatment by means of BUT(tear film break-up time), Schirmer's test, which have been considered to be typical methods for diagnosing dry eye syndrome. Methods : The subjects of this trial included the outpatients who had been diagnosed with dry eye syndrome in Pusan national university hospital and underwent acupuncture treatment from March 2011 to April 2011. They received acupuncture treatment once a week, three times while constantly using Restasis or artificial tears. We collated and analyzed the outcome data of Schirmer's test and BUT conducted at the beginning of this trial and three times more after each acupuncture treatments. Acupoints, such as, Chanjuk(BL2), Eoryo, Sajukgong(TE23), Taeyang, Sabaek(ST2), limited around both eyes were selected based on their clinical effects referring to medical books. Results : 1. From BUT test results, Restasis and acupuncture combined group showed statistically significant difference, however, artificial tears and acupuncture combined group showed difference only between before and after the treatment, which is not statistically significant. 2. From Schirmer's test results, Restasis and acupuncture combined group showed statistically significant difference, however, artificial tears and acupuncture combined group showed difference only between before and after the treatment, which is not statistically significant. 3. The average duration of treatment was statistically 16.3 days for Restasis and acupuncture combined group. Conclusions : Combination of acupuncture and Restasis for dry eye syndrome can shorten the duration of treatment. Therefore, clinical benefit is expected to be worth.

돌발성난청으로 인한 耳鳴환자 4례에 大陷胸湯을 투여한 증례보고

조형래,황보민,Jo, Hyung-Rae,Hwangbo, Min 대한한방안이비인후피부과학회 2015 한방안이비인후피부과학회지 Vol.28 No.3

Objective : The purpose of this study was to report a significant improvement of Tinnitus with Sudden sensorineural hearing loss(SSHL) after treatment with Daehamhyung-tang.Methods : We experienced four cases of tinnitus with SSHL treated by Daehamhyung-tang. To evaluate the results of this treatment, we used the visual analogue scale(VAS)Results : After using Daehamhyung-tang, VAS and clinical symptoms of 3 patients were improved remarkably, but 1 case was no interval change.Conclusion : This study suggests that using Daehamhyung-tang is effective on tinnitus with SSHL.