국산 프로폴리스의 Helicobacter pyrori 억제효과

한상미,홍인표,우순옥,김세건,장혜리,장재선,Han, Sang Mi,Hong, In Phyo,Woo, Soon Ok,Kim, Se Gun,Jang, Hye Ri,Jang, Jae Seon 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.1

Propolis possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of this study was to evaluated chemical composition and in vitro anti-Helicobacter pylori activity of eight propolis ethanolic extracts form different region in Korea. The chemical composition of eight Korean propolis was investicated by absorption spectrophotomety and UHPLC. Total penolic and total flavonoids content ranged between $21{\pm}1.5$ and $31{\pm}1.8$ gallic acid g/100 g and $7.2{\pm}0.9$ and $24.4{\pm}1.9$ quercetin g/100 g, respectively. The p-coumaric acid as standard components content ranged between $0.29{\pm}0.00$ and $2.34{\pm}0.02mg/g$ using UHPLC. Cinnamic acid content between $0.43{\pm}0.00$ and $1.33{\pm}0.01mg/g$. 80% ethanolic extract of propolis against H. pylori was evaluated by using the disc diffusion method. Ethanol was used as a control. Mean diameters of H. pylori growth inhibition using $10{\mu}g/mL$, $50{\mu}g/mL$ or $100{\mu}g/mL$ ethanol propolis per disc were 0, ranging from 0.9 to 2.1 mm and from 1.9 to 3.5 mm, respectively. In conclusion, variation in the chemical composition of Korean propolis were mainly due to the difference in the preferred regional plants visited by honeybees. Also Korean propolis possesses considerable antibacterial activity against H. pylori. There was not confirmed a correlation between the chemical composion contens and anti-H. pylori effect. The potential of propolis in the prevention or treatment of H. pylori infection is worth further extensive evaluation.

정제봉독을 함유한 봉독화장품의 피부주름 개선 효과

한상미 ( Sang Mi Han ),홍인표 ( In Phyo Hong ),우순옥 ( Soon Ok Woo ),천성남 ( Sung Nam Chun ),한충섭 ( Chung Sub Han ) 한국미용학회 2015 한국미용학회지 Vol.21 No.2

In the last few decades, there has been a substantial increase in the population of aged people. Aging skin is a common concern for many people theses days. The purpose of this study was to evaluate the efficacy of serum containing purified bee venom in reducing wrinkles. Bee venom serum was applied to the face after toner of 22 subjects twice a day for 12 weeks The changes of the skin wrinkles were analyzed with the visual grade of wrinkle and the image of the skin replica. Compared to before and after treatment, the visual grade of wrinkle was improved 11.83% after 12 weeks. The wrinkle squares and depths were significantly reduced after 12 weeks(p<0.05). Also, the wrinkle counts were significantly reduced after 12 weeks(p<0.5). Adverse reactions of subjects were not observed during the course of the study. These observations indicate that the treatment of purified bee venom seems to be effective in improving wrinkles and can be usefully tho the anti-aging cosmetic industry.

정제봉독의 반복 첩포 피부자극시험

한상미(Sang Mi Han),홍인표(In Phyo Hong),우순옥(Soon Ok Woo),김세건(Se Gun Kim),장혜리(Hye Ri Jang),박관규(Kyun Kyu Park) 대한약학회 2017 약학회지 Vol.61 No.1

This study was performed to examine the repeated skin irritation reaction of purified bee venom (Apis mellifera L.) collected using bee venom collector. To confirm whether the purified bee venom causes primary skin irritation when used for the skin medicinal products, skin irritation testing was conducted using rabbit models. The purified bee venom (0.5 mg/kg/day) was uniformly applied on each gauze patch cut into a size of approximately 2.5 cm×2.5 cm. Then, each patch was pasted to the skin, respectively, and was fixed with an adhesive plaster. The left was controls and the right was purified bee venom for 7 days everyday. Observations were made at 1, 24, 48 and 72 hours after the patch had been removed, and then the irritation reaction was scored according to Formation of erythema and eschar and edema. No irritation reaction was observed in any of the test skins during the observation period. Therefore, the P.I.I, was 0. As a result, no irritation reaction was observed in any of the test skins during the observation period. Therefore, in the primary skin irritation test using rabbits, the purified bee venom was judged to belong to a category “non-irritant”. This study demonstrated that purified bee venom has promising potential external treatment for topical uses that do not induce significant levels of skin reaction.

천연꿀과 사양꿀의 성분 분석

김세건 ( Se Gun Kim ),홍인표 ( In Phyo Hong ),우순옥 ( Soon Ok Woo ),장혜리 ( Hye Ri Jang ),장재선 ( Jae Seon Jang ),한상미 ( Sang Mi Han ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.1

In this study, we investigated and compared some chemical properties of Korean natural honeys and sugar-fed honeys for their quality characteristics. The natural honey samples were monofloral from chestnut and acacia flowers, and the sugar-fed honey samples were collected from honeybees feeding on sugar cane and sugar beet. The chemical properties of the honey samples, such as moisture, total protein, total lipids, ash, carbohydrate, minerals, vitamins, and free amino acids were determined. The moisture content was 18.5±0.9% in natural acacia honey, 17.2±0.9% in natural chestnut honey, 19.6±0.9% in sugar cane-fed honey, and 24.8±% in sugar beet-fed honey. The total protein and ash contents were the highest in natural chestnut honey. Maltose and sucrose were not detected in natural honeys but were detected at 2~7% in sugar-fed honeys. The vitamin, mineral, and free amino acids contents of natural honeys were higher than sugar-fed honeys. The natural chestnut honey is the highest in honeys. These results confirmed that the quality of natural honey was better than that of sugar-fed honey. Also, the vitamin, mineral, and free amino acids contents are potential characteristics for distinguishing between natural and sugar-fed honeys.

설탕 사양꿀의 특이 성분 분석

김세건(Se-Gun Kim),홍인표(In-Phyo Hong),우순옥(Soon-Ok Woo),장혜리(Hye-Ri Jang),한상미(Sang-Mi Han) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.4

천연꿀과 사양꿀의 성분 특성을 알아보기 위하여 천연 아카시아꿀, 밤꿀 그리고 잡화꿀을 대상으로 하였고, 사양꿀은 사탕수수 사양꿀 그리고 사탕무 사양꿀을 시료로 분석하였다. 사양꿀은 유기용매로 추출하여 단일물질을 분리하고, 이화학 분석을 통해 트랜스-2-데센다이산으로 동정하였다. 또한 UPLC를 이용하여 천연꿀과 사양꿀에서의 트랜스-2-데센다이산 함량을 분석한 결과, 사탕수수 사양꿀과 사탕무 사양꿀은 121±5.9, 127±4.5 mg/100 g 확인된 반면 천연 아카시아꿀에서는 13±0.9 mg/100 g, 밤꿀은 17±0.6 mg/100 g, 그리고 잡화꿀에서는 13±1.3 mg/100 g로 사양꿀에 비하여 상대적으로 매우 적게 포함되어 있음을 확인하였다. 이러한 결과로 보아 트랜스-2-데센다이산 성분은 사양꿀에서는 주요 성분이지만 천연꿀에서는 미량으로 존재하는 것으로 확인되었으며. 천연꿀과 사양꿀을 구분 할 수 있는 성분으로 가능성이 있는 것으로 판단되었다. We investigated and compared certain chemical properties of Korean natural honey and sugar-fed honey for assessing quality characteristics. The specification component was extracted using an organic solvent, and a single substance was isolated and identified as (E)-2-decenedioic acid. The content of (E)-2-decenedioic acid was 121±5.9 mg/100 g in sugar cane-fed honey and 127±4.5 mg/100 g in sugar beet-fed honey. Natural acacia, chestnut, and multi-floral honey contain 13±0.9, 17±0.6, and 13±1.3 mg/100 g of honey, respectively. Therefore, (E)-2-decenedioic acid was a major component of sugar-fed honey, however, it occurred in trace amounts in natural honey. We conclude that natural and sugarfed honey can be distinguished by determining the (E)-2-decenedioic acid content.

주사제 사용을 위한 봉독의 균질성 및 안정성 평가

한상미 ( Sang Mi Han ),김세건 ( Se Gun Kim ),홍인표 ( In Phyo Hong ),우순옥 ( Soon Ok Woo ),장혜리 ( Hye Ri Jang ),이경우 ( Kyung Woo Lee ) 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.2

Honeybee venom (BV) from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. The purpose of this study was conducted to confirm homogeneity and stability of BV dissolved with distilled water or saline solution. Melittin was analyzed with ultra-high performance liquid chromatography (UPLC) for BV to secure the validation of analysis. BV at concentration of 1 mg/mL was dissolved with distilled water or saline solution at room temperature. Homogeneity of BV dissolved with distilled water or saline solution at upper, milddle, and lower layers all satisfied the accuracy and precision criteria. Stability of BV dissolved with distilled water or saline solution for 7 days all satisfied the criterion both light and dark storage condition. BV has satisfied with homogeneity and stability in distilled water or saline solution at room temperature under light or dark condition. The results of this study suggest that BV has a possibility as the substitute of natural antimicrobial agents for the animal drugs and feed additives.

육계로부터 분리한 병원성 세균에 대한 봉독의 항균효과

한상미 ( Sang Mi Han ),김세건 ( Se Gun Kim ),홍인표 ( In Phyo Hong ),우순옥 ( Soon Ok Woo ),장혜리 ( Hye Ri Jang ),이경우 ( Kyung Woo Lee ) 한국가축위생학회 2016 韓國家畜衛生學會誌 Vol.39 No.3

Clostridium perfringens, Salmonella thyphimurium and S. Montevideo isolated from the intestines of dead broiler chickens in Korea were tested for antibacterial effects to purifed bee venom. Purified bee venom from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. To investigate antibacterial effect of purified bee venom was eval-uated by agar well diffusion method, minimum inhibitory concentraion (MIC), minimum bactericidal concentration (MBC), and postantibiotic effect (PAE). Purified bee venom exhibited significant in-hibition of bacterial growth of C. perfringens, S. thyphimurium and S. Montevideo with MIC value of 0.85, 0.68 and 0.69 μg/mL, respectively. The MBC value of purified bee venom against C. perfringens, S. thyphimurium and S. Montevideo were 3.33, 2.66 and 2.86 μg/mL. Furthermore, the results of PAE values against C. perfringens, S. thyphimurium and S. Montevideo showed the bacterial effect with 3.5, 4.0 and 3.5 hr. Stability of pufifed bee venom at acidity from pH 1 to pH 8 for 24 hr was the anti-bacterial activity for C. perfringens, S. thyphimurium and S. Montevideo and melittin contents. Also pu-rified bee venom processed through the heating for 15 min, there was no signification loss of the anti-bacterial activity and melittin at below 100 ℃. These results obtained in this study suggest that purified bee venom might be utilized as a feed additive in poultry diets.

산도에 따른 봉독의 성분 및 생리활성에 대한 안정성

조미란 ( Mi Ran Cho ),한상미 ( Sang Mi Han ),김정민 ( Jung Min Kim ),여주홍 ( Joo Hong Yeo ),홍인표 ( In Phyo Hong ),우순옥 ( Soon Ok Woo ),이광길 ( Kwang Gill Lee ) 한국잠사학회 2014 한국잠사곤충학회지 Vol.52 No.1

서양종 꿀벌로부터 봉독채집장치를 이용하여 채집한 정제봉독의 pH 안정성에 대하여 평가하고자 멜리틴의 함량, 단백질의 변화, 항균력, 세포증식률을 측정하였다. 정제봉독을 pH2에서 pH9까지 24시간 동안 처리한 후 멜리틴함량과 단백질의 변화, S. aureus, P. acnes, S. mutans와 E. coli에 대한 항균력, 피부세포인 HDF에 대한 세포증식률에 미치는 영향을 분석하였다. pH2에서부터 pH9까지 각각 처리한 정제봉독의 멜리틴 및 단백질 성분의 변화는 확인되지 않았다. 봉독의 주요 약리효과인 항균력에 있어서도 시험균주마다 다소 차이가 있었으나 S. aureus, P. acnes, S. mutans와 E. coli 모두 산도에 따른 유의할 만한 변화는 확인되지 않았다. 또한 피부세포 증식률에 있어서도 산도에 따른 변화는 확인되지 않았다. 이러한 결과로 미루어 봉독의 pH처리는 멜리틴 함량, 단백질의 변화, 항균력 및 세포증식률에 영향을 주지 않는 것으로 확인되어 봉독은 산도의 변화에 있어 안정성이 매우 높은 것으로 사료된다. This study was for the investigation of the stability of purified bee venom (PBV) during the treatment in the pH range from pH2 to pH9 for 24 hours, respectively. Changes of components and physiological functionalities in PBV were by evaluated silver staining, and melittin contents were measured by liquid chromatography. The antimicrobial activity against bacteria by minimum inhibitory concentration (MIC) and effect of the cell regeneration were measured by 3- [4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay using human dermal fibroblast (HDF) cell. The main proteins such as melittin and phospholipase A2 showed no characteristic changes. The antimicrobial activity and effect of cell regeneration showed no difference from pH2 to pH9. From this study, we suggest that components and physiological functionalities of PBV against treated pH were kept stability at from pH2 to pH9.