종계의 환절기별 사양관리 요령-계사 출입시 소독 철저

홍영호,Hong, Yeong-Ho 대한양계협회 2007 월간 양계 Vol.39 No.9

산처리한 γ-알루미나의 표면 산량과 표면 전하밀도

홍영호,이창우,함영민 ( Young Ho Hong,Chang Woo Lee,Yeong Min Hahm ) 한국공업화학회 1998 공업화학 Vol.9 No.3

깨끗한 닭고기를 위한 병아리 생산과정

홍영호,Hong, Yeong-Ho 한국계육협회 2008 월간 닭고기 Vol.151 No.-

산업박람회 참가자의 중요속성 인식에 관한 연구

홍영호 ( Yeong Ho Hong ),박희수 ( Hyun Soo Park ) 한국이벤트컨벤션학회 2006 이벤트 컨벤션 연구 Vol.2 No.3

The purpose of this study was to measure visitor`s perception of the importance attribute of trade exhibition. This survey suggests that the attribute limits of exhibition have to be scaled up not only the holding place but the image of holding area to make a trade exhibition become a mega event and devote all their energies to detain more tourists. This research had been conducted to know the major properties which can raise differentiation and competitive power of "the 4th Goyang World Flower Exhibition" in 2006. SPSS 12.0 for Windows was used for the samples and performed descriptive analysis and frequency analysis for data analysis and cross analysis, t-test and ANNOVA were conducted to analyze demographic variable. To verify differences of two groups it used post verification using Turkey multiple comparison method. This survey also suggests that this exhibition has to give tourist the best satisfaction and devote the growth of the regional economy. The results suggested that importance attributes including natural resource, natural environment, infra, social environment were the most importance factors. There are statistically significant differences between socio-demographic variable and 8 importance attributes of trade exhibition. Marketing and management implications for effectively targeting the segments art discussed.

Long - Term Culture and Improved Transfection Effciency of Gonadal Primordial Germ Cells (gPGCs) for the Production of Transgenic Chicken

홍영호(Yeong Ho Hong),한재용(Jae Yong Han) 한국가금학회 1998 한국가금학회 정기총회 및 학술발표회 Vol.15 No.-

6일령 배자로부터 분리한 원시생식세포 (PGC)를 IGF-1, LIF, IL-11, SCF, 그리고 bFGF등을 첨가하여 계대하지 않은 상태에서 약 3개월간 배양 하였다. 배양 약 7일경부터 PGC가 콜로니를 형성하기 시작하였는데 약 10여개의 세포들이 서로 군집형태를 이루었다. 원시생식세포의 형태학적 또는 조직학적특징들이 약 3개월 배양후에도 7일배양한 세포의 특징과 다를바 없었다. 장기배양한 원시생식세포는 mouse의 ES cell 항원인 SSEA-1, 그리고 닭의 PGC항원인 2C9에 양성적으로 반응하였으며, 배양중 약 50%의 세포들이 분열 능력이 있음을 관찰하였다. 장기 배양중 형태학적, 조직학적 특징이 murine embryonic stem cell과 유사함을 알 수 있었다. 전기영동법에 의한 유전자전이법을 liposome방법과 비교하였을 때, 전기영동법의 효율 (80%)이 liposome (17%)에 비해 월등히 높았다. 유전자 전 이후 Ficoll을 이용하여 분리한 gPGC 약 200여개를 2.5일령 (stage 17)의 배자혈관에 주입하여 추가로 3.5일(stage 29), 7.5일(stage 36) 그리고 부화시킨 후 외래 유전자 (pCMVβ) 도입 유무를 PCR 기법을 이용하여 확인 한 결과 6일령, 10일령, 그리고 부화한 병아리에서 각각 100%, 67%, 41%의 효율로 외래 유전자가 도입되었을 알 수 있었다. 특히 부화한 병아리의 경우 외래 유전자가 생식기에서만 발견됨을 알 수 있었다.<br/> 따라서 이러한 결과는 주입한 원시생식세포가 수용체의 원시생식기에 도달한 후 생식기 형성에 기여하였으며, 형질전환 닭 생산을 위한 유용한 벡터로서 사용될 수 있는 가능성을 제시하였다. Primordial germ cells (PGCs) were isolated from the gonad of 6 days old embryos and cultured in the presence of IGF-1, LIF, IL-11, SCF, and bFGF for 3 months without passage. The gPGCs started to form colonies composing about 10 gPGCs after 7 days culture. The morphological and histochemical characteristics of gPGC cultured for 3 months remained same compared to those of gPGCs cultured for 7 days. The long-term cultured gPGCs expressed the murine ES cell specific epitopes, SSEA-1, and 2C9, which is chicken PGC-specific antibody. About 50% of the PGC population showed the capacities of proliferation during culture period. The morphological and histochemical characteristics of PGCs were maintained for long term culture period and the epitope profiles were similar to those of murine embryonic stem cells. When electroporation of gPGCs was compared to the use of liposomes, electroporation resulted in an 80% transfection efficiency compared to about 17% observed with liposomes. Approximately 200 transfected gPGCs were injected into 2.5 day old (stage 17) recipient embryos and the eggs were incubated for an additional 3.5 days, 7.5 days or to hatching. The percentages having exogenous gene were 100%, 67% and 41% in the 6 day old (stage 29), 10 day old (stage 36) recipient embryos and hatched chicks gonads, respectively. PCR analysis of DNA from the hatched chicks showed that exogenous lacZ DNA was detected only in the gonad and not in the liver and heart.<br/> These results indicate that gPGCs can contribute to gonadal formation after transfer to recipient embryos and be used as a useful vector for the genetic manipulation to produce the transgenic chicken.

Alternatives to antibiotics and immune modulating network to disease control

Yeong Ho Hong(홍영호) 한국가금학회 2017 한국가금학회 정기총회 및 학술발표회 Vol.2017 No.11

주요 병원균 자극에 의한 닭의 Interleukin-34 발현 분석 비교

홍영호(Yeong Ho Hong) 한국가금학회 2021 韓國家禽學會誌 Vol.48 No.3

최근에 IL-34가 MCSFR에 대한 두 번째 기능적 리간드로 확인되었으며, M-CSFR에 대한 결합을 통해 IL-34는 M-CSF와 유사한 기능을 공유한다. 지금까지 닭의 IL-34에 대한 실험적 연구가 아닌 예측된 서열로만 보고되었으며, 닭의 IL-34 기능에 대한 정보는 아직 부족하다. 닭 IL-34의 잠재적 생물학적 기능을 구명하기 위하여, 다양한 품종의 닭, 세포주에 대한 괴사성 장염, 살모넬라 및 E. coli 유래의 LPS 등을 처리하여 관찰하였다. 또한 닭의 IL-34와 M-CSF 재조합 단백질 생산 및 이를 이용한 전염증성 사이토카인 유도를 위하여 클로닝 및 재조합 단백질을 발현 및 정제하였다. 특히 관절이상 육계(unknown cause)에 대한 IL-34, M-CSF, 그리고 M-CSFR 유전자의 발현이 대조구보다 유의하게 증가하였으나, 병원균 자극 조직의 경우 많은 조직에서 감소함을 보였다. M-CSFR의 발현은 in vitro에서 IL-34와 M-CSF 재조합 단백질에 의해 증가함을 보였으며, IFN-γ은 재조합 단백질 M-CSF을 제외한 IL-34에 의해 증가하였다. 하지만, IL-12 발현은 유의적인 차이가 없었으며, IL-1β의 경우는 감소하였다. 이 결과는 IL-34와 M-CSF가 고전적인 대식세포와 대체 대식세포 모두에서 역할을 한다고 할 수 있다. 결론적으로, in vitro와 in vivo 실험을 통하여 병원균 처리 시 닭의 IL-34 발현을 관찰하였으며, IL-34 재조합 단백질이 대식세포에서와 전염증성과 항염증성 반응에 중요한 역할을 함을 증명하였다. 추후에 IL-34의 사이토카인, 케모카인 그리고 염증반응 경로 등에 대한 추가 연구가 필요하다고 판단된다. Recently, interleukin 34 (IL-34) was identified as the second functional ligand for macrophage colony-stimulating factor receptor (M-CSFR). IL-34 functions similarly to M-CSF through its binding to the M-CSFR. There is still insufficient information on IL-34 in chickens, which has until now been reported only through predicted sequences and not through experimental research. Thus, to confirm its expression and to determine its potent biological activity, several chicken lines and cell lines were used. Cloning of recombinant chicken IL-34 and M-CSF genes was performed to investigate their modulatory effects on proinflammatory cytokine expression in vitro. The expression levels of IL-34, M-CSF, and M-CSFR genes were upregulated in broiler chickens with leg dysfunction (cause unknown). However, IL-34 was downregulated in most pathogen-stimulated tissues. M-CSFR expression was enhanced by recombinant IL-34 and M-CSF proteins in vitro. IFN-γ expression was enhanced by recombinant IL-34, but not by M-CSF. However, IL-12 expression was not regulated in any of the treated cells, and IL-1β was decreased in all tissues. These results indicate that IL-34 and M-CSF have roles in both the classical and alternative macrophage activation pathways. Collectively, our findings demonstrate the expression of IL-34 in chickens for pathogenic trials, both in vitro and in vivo. Our results suggest that the IL-34 protein plays a role in both pro- and anti-inflammatory functions in macrophages. Therefore, further research is needed to determine the cytokines or chemokines that can be induced by IL-34 and to further elucidate the functions of IL-34 in the inflammatory pathway.

항공사 프로모션 혜택이 여행사의 관계몰입과 장기협력관계에 미치는 영향

홍영호 ( Yeong Ho Hong ),이진호 ( Jin Ho Lee ) 관광경영학회 2013 관광경영연구 Vol.56 No.-

This study aims to examine the effects of promotion benefits of airlines on relational commitment, long-term cooperative relationship between airlines and travel agencies. And the results of research also aim to provide practical implications that can be used as a basic data to enhance the competitiveness of airline`s sales promotion. This study is based on previous literature reviews of promotion benefit, relational commitment and long-term cooperative relationship. As the results of the factor analysis, promotion benefit of airlines is composed of two dimensions of monetary benefit and non-monetary, relational commitment is consisted of affective commitment and calculative commitment. While long-term cooperative relationship is consisted of a single dimension. As the results of rectifying hypothesis, monetary and non-monetary benefit have a significant relationship with affective and calculative commitment respectively. And affective and calculative commitment also have a significant relationship with long-term cooperative relationship. The implications of this study based on the results of the empirical analysis are as follows. Firstly, airlines should strengthen promotion plans to provide monetary for travel agencies. Secondly, it is necessary to improve mutual trust and closer relationship between airlines and travel agencies. Thirdly, airlines should consider travel agencies as a business partner with close ties than short-term relationship.

Production of Germline Chimeric Chickens by Transfer of Cultured Primordial Germ Cells

홍영호(Yeong Ho Hong),정동기(Dong Kee Jeong),박태섭(Tae Sub Park),김양하(Yangha Kim Moon),한재용(Jae Yong Han) 한국가금학회 1997 한국가금학회 정기총회 및 학술발표회 Vol.14 No.-

질산이온의 제거에 미치는 알루미나 표면활성의 영향

홍영호(Young Ho Hong),함영민(Yeong Min Hahm) 大韓環境工學會 1995 대한환경공학회지 Vol.17 No.5