Raw 264.7 macrophages에 대한 Pyrrosia lingua 추출물의 항염증 효과

홍신협(Shin Hyub Hong),이은호(Eun-Ho Lee),박혜진(Hye-Jin Park),김정인(Jung In Kim),정희영(Hee-Young Jung),강인규(In-Kyu Kang),김병오(Byung-Oh Kim),박희동(Heui-Dong Park ),조영제(Young-Je Cho) 한국식품저장유통학회 2021 한국식품저장유통학회지 Vol.28 No.6

옥광(Castanea crenata) 밤송이 추출물의 생리활성 효과

이은호(Eun Ho Lee),홍신협(Shin Hyub Hong),조영제(Young Je Cho) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.5

옥광(Okkwang; Castanea crenata) 밤송이 고형분의 phenolic 화합물의 함량은 물, 40% 에탄올 추출물에서 각각 11.24, 10.28 μg/50 μg solid로 측정되었다. DPPH 라디칼 소거능은 물, 에탄올 추출물 50 μg/mL solid 농도에서 각각 85, 87%의 전자공여능을 나타내었고, ABTS 라디칼 소거능은 물, 에탄올 추출물에서 각각 100, 86%의 전자공여능을 나타내었다. PF에서는 물, 에탄올 추출물 200 μg/mL solid 농도에서 각각 1.22, 1.45 PF를 나타내었고, TBARS는 물 추출물에서 83%, 에탄올 추출물에서는 73%의 효능을 나타내었다. XOase 저해 효과는 물, 에탄올 추출물 200 μg/mL solid 농도에서 각각 54, 43%를 나타내었다. α-Glucosidase 저해 효과는 50 μg/mL solid 농도에서 물 추출물은 95%, 에탄올 추출물에서는 96%의 저해 효과를 나타내었다. 미백을 나타내는 tyrosinase 저해 효과는 200 μg/mL solid 농도에서 에탄올 추출물은 27%의 효능을 나타내었다. 주름개선을 보여주는 collagenase 저해 효과는 200 μg/mL solid 농도에서 물, 에탄올 추출물 각각 93, 94%의 우수한 주름개선 효과를 나타내었으며, elastase 저해 효과는 200 μg/mL solid 농도에서 에탄올 추출물은 56%의 효능을 나타내었다. 항염증 효과를 나타내는 HAase 저해 효과를 측정한 결과 200 μg/mL solid 농도에서 물, 에탄올 추출물에서 각각 96, 52%의 항염증 효과를 나타내었다. 이러한 결과로 보아 OCS 추출물은 항산화 활성, 통풍 억제, 항당뇨, 미백, 주름개선, 항염증 효과가 우수하므로 기능성 소재로서 사용이 가능할 것으로 기대된다. The contents of phenolic compounds in water and 40% ethanol extracts from Okkwang (Castanea crenata) chestnut bur solid (OCS) were 11.24 μg/50 μg solid and 10.28 μg/50 μg solid, respectively. The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging and 2,2’-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid) radical decolorization activities of water and ethanol extracts were 85% and 100% as well as 87% and 86% at a solid content of 50 μg/mL, respectively. The anti-oxidant protection factors (PFs) of water and ethanol extracts at a solid content of 200 μg/mL were 1.22 PF and 1.45 PF, respectively. Thiobarbituric acid reactive substance were 83% in water extract and 73% in ethanol extract at a solid content of 200 μg/mL. The inhibitory activities against xanthine oxidase in water and ethanol extracts were 54% and 43% at a solid content of 200 μg/mL, respectively. The inhibitory activities against α-glucosidase were 95% in water extract and 96% in ethanol extract at a solid content of 50 μg/mL. Tyrosinase inhibitory activity was 27% in ethanol extract at a solid content of 200 μg/mL. The collagenase and elastase inhibitory activities as anti-wrinkle effect were 93% and 11% in water extract as well as 94% and 56% in ethanol extract at a solid content of 200 μg/mL. Hyaluronidase inhibitory activity as anti-inflammatory effect of water and ethanol extracts were 96% and 52% at a solid content of 200 μg/mL, respectively. The results show that extracts from OCS can be used as a functional resource with antioxidant, anti-gout, carbohydrate degradation inhibitory, whitening, anti-wrinkle, and anti-inflammatory activities.

[철회논문] 말오줌나무(Sambucus sieboldiana var. pendula) 잎 유래 phenol성 물질의 생리활성 효소 저해 및 항균효과

이은호 ( Eun-ho Lee ),홍신협 ( Shin Hyub Hong ),박혜진 ( Hye-jin Park ),김병오 ( Byung-oh Kim ),정희영 ( Hee-young Jung ),강인규 ( In-kyu Kang ),조영제 ( Young-je Cho ) 한국응용생명화학회 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.1

The phenolics of Sambucus sieboldiana var. pendula leaves for functional resources were examined on inhibitory activity against biological enzyme and anti-microbial activity. The amount of phenolic compounds were 11.60±0.18 and 12.43±0.07 mg/g by water and 50% ethanol extraction, respectively. The antioxidative activity of phenolic in extracts was higher than solids. The inhibition activities on angiotensin converting enzyme were 92.08 and 78.33% at 200 μg/mL phenolic concentration in water and ethanol extracts, respectively. The xanthine oxidase inhibitory activity were 100% at 200 μg/mL phenolic in water and ethanol extracts, respectively. These result was higher than 70.37% of allopurinol as positive control at 200 μg/mL. The inhibitory activity against hyaluronidase were each 25.35 and 43.38% in water and ethanol extracts. The water extract from S. sieboldiana var. pendula leaves showed antibacterial activity on the Propione-bacterium acnes, Staphylococcus aureus and Streptococcus mutans in water extract and S. mutans in ethanol extract. This result suggests that phenolic from S. sieboldiana var. pendula leaves are suitable as functional foods with anti-hypertension, anti-gout, anti-inflammation and anti-microorganism activities.

한약재 장기 보존을 위해 감마선 조사한 독활(Aralia continentalis) 추출물의 항산화 효과 비교

박혜진(Hye-Jin Park),홍은진(Eun-Jin Hong),홍신협(Shin-Hyub Hong),조영제(Young-Je Cho) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.1

본 연구에서는 한약재의 장기보존을 위한 위생처리법으로 잘 알려진 감마선 조사 처리 시 독활 추출물의 항산화 활성에 미치는 영향을 검토하였다. 천연 약용식물인 독활의 감마선 조사 처리에 따른 조사 판별 marker 확인을 위해 광자극 발광법, 열발광분석법, 전자스핀공명법을 이용하여 확인시험법의 판별 특성을 확인하였다. 그 결과 광자극발광법에서 비조사구 0 kGy에서 891.00 PCs의 중간시료 수치를 나타내어 광자극발광법 단독으로는 조사 여부 판별 확인이 어려운 것을 확인할 수 있었다. 하지만 열발광분석법, 전자스핀 공명법에서는 조사구와 비조사구 간의 차이를 명확히 확인할 수 있었으며, 이를 통해 한약재인 독활은 3가지 확인 시험법을 모두 적용하여 분석하는 것이 적합한 것으로 판단되었다. 에탄올 농도별 추출에서는 50% 에탄올에서 비조사구 0 kGy와 조사구 10 kGy에서 각각 5.98 mg/g, 6.86 mg/g의 함량을 나타내었다. 항산화 활성 확인을 위해 추출물의 phenolic 농도를 50~200 μg/mL로 조절하여 DPPH radical 소거 효과를 측정한 결과 열수와 50% 에탄올 추출물의 비조사구 0 kGy와 조사구 10 kGy 50~200 μg/mL phenolic 농도 전 구간에서 84.04~100.00%의 매우 높은 소거효과를 나타내었으나, 감마선 조사 처리에 따른 유의적인 차이는 없었다. ABTS radical 소거 활성 측정 결과 열수와 50% 에탄올 추출물의 비조사구 0 kGy와 조사구 10 kGy 200 μg/mL phenolic 농도에서 95% 이상의 높은 소거 효과를 나타내었다. Antioxidant protection factor(PF) 측정 결과 100 μg/mL phenolic 농도 처리 시 열수 추출물 비조사구 0 kGy와 조사구 10 kGy에서 각각 2.51 PF, 2.01 PF, 50% 에탄올 추출물 비조사구 0 kGy와 조사구 10 kGy는 각각 1.38 PF, 1.48 PF를 나타내었다. TBARs 저해 효과는 50~200 μg/mL phenolic 농도 전 구간에서 열수 추출물 비조사구 0 kGy와 조사구 10 kGy에서 65.23~83.10%를 나타냈으며, 50% 에탄올 추출물 비조사구 0 kGy와 조사구 10 kGy에서 70.67~95.81%로 농도 의존적으로 증가하는 양상을 나타냈다. 본 연구 결과 감마선 조사 처리에 따른 total phenolic compounds 함량은 열수 추출물의 경우 조사 처리 후 함량이 감소하였고, 50% 에탄올 추출물의 경우 조사 처리 후 함량이 증가하는 것을 확인할 수 있었다. 독활의 장기저장을 위해 처리한 감마선 조사 전과 후 독활 추출물의 항산화 활성 변화에는 큰 영향을 주지 않는 것으로 보이며 이는 감마선 조사법이 독활을 포함한 한약재의 장기저장을 위한 처리법으로 유용하게 이용될 수 있을 것으로 판단된다. In this study, extracts of gamma-irradiated Aralia continentalis for health and beauty food resources were examined on antioxidant activities and increasing extraction yield. In photostimulated luminescence analysis, non-irradiated (0 kGy) sample showed an intermediate result of 891.00 photon counts (PCs), whereas irradiated (5 and 10 kGy) samples showed positive results of 79,956.67 and 282,498.67 PCs, respectively. The thermoluminescence ratio of non-irradiated samples was 0.018, whereas the values of irradiated samples (5 and 10 kGy) were 0.395 and 0.464, respectively. Electron spin resonance (ESR) analysis as a physical technique for irradiation identification of Aralia continentalis showed a pair of peaks on a space of 6.0 mT in the symmetric ESR spectrums. For phenolics of gamma-irradiated Aralia continentalis, water extracts had the highest amounts of 7.61 mg/g at non-irradiated condition (0 kGy) while 50% ethanol extracts had the highest amounts of 6.86 mg/g at 10 kGy irradiation. 2,2-Diphenyl-1-picrylhydrazyl scavenging activity of water and 50% ethanol extracts from non-irradiated and irradiated Aralia continentalis were very high (94.99∼100.00%). 2,2’-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation inhibitory activity was over 95.00% at 200 μg/mL phenolics. Antioxidant protection factor (PF) showed a very high level of activity (1.38∼2.51 PF). Thiobarbituric acid reactive substance inhibitory activity was high (70% and 95%, respectively). Thus, treatment of gamma irradiation can be used as a method of storage for long-term preservation of Oriental medicine.

Silane계 유무기 하이브리드 적용 합금도금강판 내식성 향상 코팅 기술 개발

박종원 ( Jong Won Park ),이경황 ( Kyung Hwang Lee ),박병규 ( Byung Kyu Park ),홍신협 ( Shin Hyub Hong ) 한국부식방식학회(구 한국부식학회) 2013 Corrosion Science and Technology Vol.12 No.6

Silane surface treatments have been developed as an alternative for toxic and carcinogenic chromate-based treatments for years. It is consistently observed that ultra-thin films offer excellent corrosion protection as well as paint adhesion to metals. The silane performance is comparable to, or in some cases better than, that of chromate layers. Based on the tetra-ethylorthosilicate(TEOS) and methlyl trieethoxysilane(MTES), inorganic sol was synthesized and formed hybrid networks with SiO2 nano particle and polypropylene glycol(PPG) on Zn alloyed steel surface. According to SST results, addition of 10nm and 50nm SiO2 nanoparticle in synthesized solution improved anti-corrosion property by its shear stress relaxation effect during curing process. Also, SST results were shown that anti-corrosive property was affected by the amounts of organic compounds.

건조방법에 따른 잣나무(Pinus koraiensis) 잎 추출물의 주름개선 및 미백 효과

조재범 ( Jae-bum Jo ),박혜진 ( Hye-jin Park ),이은호 ( Eun-ho Lee ),이재은 ( Jae-eun Lee ),임수빈 ( Su-bin Lim ),홍신협 ( Shin-hyub Hong ),조영제 ( Young-je Cho ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.1

본 연구는 잣나무(Pinus koraiensis) 잎을 열풍건조, 음지건조, 동결건조하여 물과 80 % ethanol을 추출용매로 사용하여 추출물이 미용식품의 소재로서의 기능성을 증명하고자 실시하였다. Total phenolic compounds는 WE에서는 17.93 mg/g로 음지건조가 가장 높았고, EE에서도 20.63 mg/g로 음지건조 추출물이 가장 높은 용출량을 나타내었다. DPPH, ABTS radical 전자공여능 측정 결과, WE는 열풍건조에서 90.45, 99 %, EE에서는 음지건조가 96.2, 99%의 높은 활성을 나타내었다. PF 측정 결과, 음지건조 잣나무 잎 추출물에서 WE는 9.63 PF, EE에서는 10.48 PF의 결과를 나타내었고, TBARs 측정 결과, 동결건조 잣나무 잎의 WE에서 82.07 %와 음지건조 잣나무 잎의 EE에서는 89.39%의 가장 높은 높은 활성을 나타내었다. 주름개선 효과를 측정 결과, elastase 저해 활성은 동결건조 잣나무 잎 EE에서 71.46 %, collagenase 저해 활성은 음지건조 잣나무 잎의 EE에서 97.48 %로 가장 높은 저해 활성을 나타내었다. Melanin 생합성을 억제할 수 있는 tyrosinase 저해 활성은 음지건조 잣나무 잎의 EE 저해 활성이 63.03 %로 가장 높았다. 이러한 결과로 폐자원으로 여겨지는 잣나무 잎을 음지건조 하였을 때 항산화능뿐만 아니라, 주름 및 미백 활성이 특히 높은 것으로 보아 향후 잣나무 잎이 기능성 미용 소재 산업에 활용가치를 기대할 수 있었다. This study provide activity for beauty food of water and 80 % ethanol extracts from Pinus koraiensis leaves. Total phenolic content of extracts from Pinus koraiensis leaves were each 12.22 mg/g (Drying under hot air) and 17.93 mg/g (Drying under shade), 14.36 mg/g (Lyophilization) in water extracts (WE) and 11.9 mg/g and 20.63 mg/g, 17.96 mg/g in 80 % ethanol extracts (EE). The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity of extracts from Pinus koraiensis leaves was 96.20 % in EE from drying under shade at extracts concentration. The 2,2`- azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical decolorization activity of extracts from drying under shade was 99.85 % in WE and 99.80 % in EE at extracts concentration. The antioxidant protection factor (PF) extracts from drying under shade type was 9.63 PF in WE and 10.48 PF in EE at extracts concentration. The thiobarbituric acid reactive substance from Pinus koraiensis leaf was 89.39 % in EE from drying under shade at extracts concentration. The elastase inhibition activity of EE for anti-wrinkle effect showed an excellent wrinkle improvement effect, showing 71.46 % in EE from lyophilization. Collagenase inhibition activity of EE from drying under shade was 97.48 % in extracts. Tyrosinase inhibition activity which was related to antimelanogensis was observed. The tyrosinase inhibitory effect of extracts from lyophilization was confirmed to be 60.4 % in EE more than another drying methods at extracts concentration. Through out all results, it can be expected Pinus koraiensis leaves extracts to use as a functional material for anti-oxidant and functional beauty food.

합금도금강판 내식성 향상을 위한 UV 경화형 유무기 하이브리드 코팅

박종원 ( Jong Won Park ),이경황 ( Kyung Hwang Lee ),나현주 ( Hyun Ju Na ),박병규 ( Byung Kyu Park ),남영섭 ( Yeung Sub Nam ),홍신협 ( Shin Hyub Hong ) 한국부식방식학회(구 한국부식학회) 2013 Corrosion Science and Technology Vol.12 No.1

According to its merits about high curing speed and low emission of volatile organic compounds. UV curable inorganic-organic coating technology has been developed as an alternative for toxic and careinogenic chromate-based treatments for years. It is consistently observed that ultra-thin films ofli2r excellent corrosion protection as well as paint adhesion to metals. Based on the tetra-ethylorthosilicate(TEOS) and methacrylox-ypropyl trimethoxysilane(MPTMS), inorganic sol was synthesized and formed hybrid networks with UV curable acrylic monomer. 6-hexanediol diacrylate(HDDA). trimethylolpropane triacrylate(TMPTA), pentaerylthritol triacrylate(PETA). Several methods were used to test their properties such as salt spray test. potentiodynamic measurement, tape peel test. etc. It was shown that anti-corrosive property and stability of storage were affected by the molecular ratios of inorganic and organic compounds. It was not only the stability of storage. but had a excellent anti-corrosive, paint adhesive, and anti-solvent properties in a final molar ratios of 0.6/0.04/0.86/0.005 (TEOS/MPTMS/Acetone/HNO3) and 0.08/0.106/0.081/0.02