드럼스틱 잎 추출물의 항산화 및 세포보호 효과

현송화 ( Song Hua Xuan ),김아랑 ( A Rang Kim ),정윤주 ( Yoon Ju Jeong ),이난희 ( Nan Hee Lee ),박수남 ( Soo Nam Park ) 대한화장품학회 2016 대한화장품학회지 Vol.42 No.3

본 연구에서는 드럼스틱 잎 추출물과 분획들의 항산화 활성 및 HaCaT 세포와 적혈구 세포에서의 세포보호효과를 측정하였다. 모든 실험은 드럼스틱 잎의 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 이용하였다. 1,1-Diphenyl-2-picrylhydrazyl radical을 이용한 자유 라디칼 소거 활성(FSC₅₀)은 50% 에탄올 추출물(77.10 μ g/mL) < 에틸아세테이트 분획(20.63 μ g/mL) < 아글리콘 분획(17.00 μ g/mL) 순으로 증가하였다. 루미놀을 이용한 Fe³⁺-EDTA/H₂O₂계에서의 활성산소 소거 활성(총항산화능, OSC₅₀)은 아글리콘 분획의 OSC₅₀ 값이 0.63 μ g/mL로 추출물 중 가장 큰 항산화능이 나타났으며, 이는 L-ascorbic acid (1.50 μ g/mL)의 항산화 활성보다 컸다. ¹O₂로 유도된 적혈구 세포 손상에 있어서 50% 에탄올 추출물 및 아글리콘 분획의 세포 보호 효과(τ₅₀)는 10 μ g/mL에서 각각 46.9 및 122.1 min을 나타냈다. 이는 지용성 항산화제로 알려진 (+)-α-tocopherol (37.7 min)보다도 훨씬 큰 세포 보호 활성을 보여주었다. 400 mJ/㎠의 UVB를 HaCaT 세포에 조사하여 세포손상을 유도한 후 에틸아세테이트 분획 및 아글리콘 분획은 0.20 ∼ 1.56 μ g/mL 농도에서 농도 의존적으로 세포보호효과를 나타내었다. 이상의 결과들은 자외선에 노출된 피부에서 드럼스틱잎 추출물과 분획들이 ROS 소거를 통하여 세포를 보호함으로서 화장품에서 천연 항산화제로서 사용 가능함을 시사하였다. In this study, we investigated the antioxidative and cellular protective effects on HaCaT cells and erythrocytes of Moringa oleifera (M. oleifera) leaves extract and its fractions. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction of M. oleifera leaves. The free radical scavenging activity (FSC₅₀) of the extract and fractions of M. oleifera leaves were in the following order: 50% ethanol extract (77.10 μg/mL) < ethyl acetate fraction (20.63 μg/mL) < aglycone fraction (17.00 μg/mL) by using the 1, 1-diphenyl-2-picrylhydrazyl. In Fe³⁺-EDTA/H₂O₂ system using the luminol, reactive oxygen species (ROS) scavenging activities (total antioxidant capacity, OSC₅₀) of aglycone fraction (OSC₅₀ = 0.63 μg/mL) was the strongest among all extracts, which was much higher than L-ascorbic acid (1.50 μg/mL). In the ¹O₂-induced cellular damage of erythrocytes, the cellular protective effects of 50% ethanol extract (τ₅₀ = 46.9 min) and aglycone fraction (τ₅₀ = 122.1 min) were higher than (+)-α-tocopherol (τ₅₀ = 37.7 min), known as a lipophilic antioxidant at 10 μg/mL. After cell damage induced by 400 mJ/cm2 UVB irradiation, the cellular protective effects of ethyl acetate and aglycone fraction of M. oleifera leaves extract were showed on the concentration from 0.20 to 1.56 μg/mL. These results suggest that M. oleifera leaves extract and its fractions can function as a natural antioxidant agent in cosmetics on skin exposed to UV radiation by protecting cellular membrane against ROS.

운동생리학 : 장시간의 운동시 항산화제섭취가 골격근의 항산화효소 활성과 심장근 및 골격근 손상의 조직형태학적 변화에 미치는 영향

현송자(SongJaHyun),정성태(SungTaiChung),유승민(SeungMinYoo) 한국체육학회 2002 한국체육학회지 Vol.41 No.4

The purpose of this present study was to investigate the effect of on antioxidants supplementaton on antioxidant enzyme activuty of skeletal and histomorpholgical change of cardiac or skeletal muscle damage during prolonged exercise in rats.Forty male Sprage-Dawley rats (4weeks old) were used as subjects. The subjects were divided into 4groups as control(CON), non-antioxidants(NAE) exercise, antioxidant control(AC) or antioxidants exercise(AE) group. Non-antioxidant group(CON & NAE) fed general food(Samyang, Inc. Korea) for 16weeks. Antioxidant group(AC & AE) fed the general food and the mixed antioxidants which were consist of vitamin E(2001U/kg/day), vitamin C(50mg/kg/day), vitamin B6(250㎍/kg/day), β-carotene(300mg/kg/day and selenomethionine(0.1mg/kg/day). After the diet experiment for 16weeks, exercise groups(NAE & AE) completed swimming exercise for 30 minute. Immediately, after swimming exercise, all the rats were sacrifced for the isolation of gastrocnemius.Then, SOD of soleus and GPx or GR enzymes of gastrocnemius muscle were assayed. Prolonged exercise no changed the SOD activity, but increaed the GPx or GR activity of skeletal muscle. Further, antioxidant supplementation more significantly increased the GPx or GR activity of gastrocnemius muscle than those of non antioxidants group. Histomorphological studies, the heart muscle damage no presented, but only the non-antioxidant exercise group swelled infiltration of inflammatory cell. In conclusion, The prolonged exercise gave rise to increase oxidative stress which led to oxidative damage, The present results clearly suggest that the antioxidant supplmentation were accelerated the glutathione enzyme activiies of skeletal muscle and help to prevent the development of work muscle damage. Further, experimentation with exercise protocols might be necessary to clarify this point.

중국 해상집법기구의 구조와 역할

玄松鶴,김운곤 한국해양경찰학회 2011 한국해양경찰학회 학술대회 Vol.2011 No.1

蛋白質 必要量이 運動에 미치는 影響

玄松子 東亞大學校 附設 스포오츠 科學硏究所 1984 스포츠科學硏究論文集 Vol.2 No.-

Four weeks old male rats of Sparague-Dawley were divided into three dietary groups (15%, 20% and 25% of dietary protein levels), and each dietary groups was further divided into sedentary and exercising groups. While the sedentary group of rats were housed in individual small cages all day long, the exercising rats were subjected to all day running and swimming at 30minutes per day. The influence of dietary protein level on the growth rate was studied. After three weeks of experimental period, the following results were obtained: 1. The body weight of sedentary group was significantly lower at 15% level of dietary casein than at both 20% and 25% levels. However, there was no significant difference between three levels. 20% level of dietary casein observed to produce most favorable in influence on maximum growth rate among three kinds of diets. 2. The body weight of exercising group was significantly higher at 25% level of dietary casein than at both 15% and 20% levels. However, there was no significant difference between three levels. Growth rate was higher in sedentary than in exercising rats, but there was a tendency that the casein level of exercising rats was higher than of sedentary rats.

트라이애슬러의 비타민Q 섭취가 호흡순환기능 및 항산화 효소활성에 미치는 영향

현송자,김귀백,박상일,석혜경 東亞大學校附設스포츠科學硏究所 2002 스포츠科學硏究論文集 Vol.20 No.-

The purpose of this present study was to investigate the effect of VitaminQ supplementation on antioxidant enzyme activities and cardiorespiratory function following prolonged exercise in trained triathlers. Male 14 healthy college students of trained triathlers participated in exercise sessions. The subjects were divided into 2 groups, as Non-supplement(NS) or VitaminQ supplement(VQ) groups. VitaminQ subjects were participated in two exercise sessions: an initial prolonged exercise(swim 750m, running 10㎞) and 70%V O2max treadmill running exercise. The Non-supplement group fed normal diet. VitaminQ group adminstated 90㎎/day for 4weeks. Venous blood samples were drown from the forearm antecubital vein pre-supplement, pre-exercise and immediately post-exercise. The results of this study can be summarized as follow: First, the SOD activity of RBC was not changed, while the GPx, GR or GR/GPx activies of Hb were significantly increased following prolonged exercise with CoQ10 supplementation. Second, 70%VO2max treadmill running exercise increased significantly the V O2max and VE, but the heart rate significantly decreased. In conclusion, the present results clearly show that the VitaminQ supplementation gave rise to increase the GPx or GR activies and to improve the cardiorespiratory function.

長吉圖開發開放先導區建設与國際合作法律問題硏究

玄松鶴 전북대학교 동북아법연구소 2012 동북아법연구 Vol.5 No.3

장길도개발개방선도구는 중국정부가 2009년에 비준실시한 유일한 중국 변경개발개방구역이고 대두만강구역국재협력과 경제발전의 중요한 구성부분이며 러시아빈해변강지구, 조선 라선지구, 몽골동부변강성시, 한국 동부연해도시 및 일본 동부연해도시의 경제발전과 개방에 밀접한 관계가 있다, 그러나 근 2년간의 장길도개발개방선도구건설 실시과정에서 여러 국가들지간의 법률방면의 협력요소의 제약하에 장길도 개발개방선도구건설의 그 진척이 더디어지고 있다. 본고는 우선 장길도개발개방선도구건설의 내용과 의의를 소개하는 기초위에서 장길도개발개방건도구건설 중 상관나라지간의 협력을 제약하는 법률요소를 분석하고 그에 다응되는 합리적이고 구체적인 법률협력시스템을 구축하자는 데 그 취지를 두고 있다.

개혁 개방이후 중국의 농업분야 국제협력

현송남 한국국제농업개발학회 1996 韓國國際農業開發學會誌 Vol.8 No.3

1978년부터 실시된 改革·開放政策은 10여년동안 지속된 文化大革命에 의해 파괴된 국가경제를 오늘의 활기에 넘치는 市場經濟로 성장시켰다. 경제개혁 실시과정에 있어서 농촌지역과 농업개혁이 生産責任制를 중심으로 실시되면서 국가경제의 발전기초를 잡게 되었으며 세계 농경지면적의 7%밖에 안되는 농경지로 세계총인구의 20% 이상되는 국민의 식량문제를 自國의 힘으로 해결하였다. 중국의 改革 開放이 오늘의 성공을 이룩하게 된 것은 自國政府와 국민 모두가 꾸준히 노력한 결과이지만 이 또한 유엔(UN)을 중심으로 한 諸國際機構와 세계 각국과의 국제협력의 결실이기도 하다. 중국농업의 생산성 향상과 農業科學技術振興事業도 諸國際機構와 세계 각국의 협력을 받으며 농업의 대혁신을 이루었다.

大数据云计算背景下网络诈骗犯罪防控对策

현송학,Pengyu Wu 인문사회 21 2017 인문사회 21 Vol.8 No.6

The development of modern network technology not only brings the rapid development of the national economy and the convenience of civic life, but also provides the criminals with the new type of crime means. Nowadays, as a new type of crime, Internet fraud shows the characteristics of complex and diverse means of crime, strong concealment and great social harmfulness. In this regard, the judiciary should build a comprehensive integration of social forces in various fields of big data cloud platform, the national networking linkage mechanism, the talent supply mechanism, which can prevent and combat crime efficiently. 现代网络技术的发达, 不仅带来国民经济的飞速发展及公民生活的便利, 也为 犯罪分子提供了新型犯罪手段. 当今, 网络诈骗犯罪, 作为新型犯罪的一种, 呈现出作案手段复 杂多样 跨国界, 跨地域, 被害人人数多, 诈骗数额特别巨大, 社会危害性极大的特点. 对此, 中 国司法机关应当全面构建融合社会各领域力量的大数据云计算平台, 推行全国联网联动机制, 建 立人才供应机制, 有效防控与打击网络诈骗犯罪.

유산소운동이 혈장 유리칼슘 농도 조절에 미치는 영향

현송자 ( Song Ja Hyun ),석혜경 ( Hae Kyoung Seok ) 한국스포츠정책과학원(구 한국스포츠개발원) 2006 체육과학연구 Vol.17 No.2

본 연구는 30분간 60%HRmax 유산소 운동이 혈장 유리칼슘 농도 조절에 부갑상선 호르몬 혹은 pH인자 중 어떤 인자가 영향을 주는가를 알아보는데 목적이 있다. 연구 대상은 건강한 남자대학생 14명을 운동군, 대조군 각각 7명씩 무작위로 나누었고 운동군은 60%HRmax 유산소 달리기 운동을 30분간 주3회 8주간을 실시하였다. 두 군 모두 60%HRmax 일회성 유산소 달리기 운동 30분을 실시하여 운동전, 운동직후, 회복기 5분, 10분, 30분에 전완에서 10ml 혈액을 채혈하였으며 이온화 칼슘과 pH는 전해질 분석기로, 총칼슘은 자동생화학분석기로, 알부민은 생화학분석기로 분석하였다. 부갑상선호르몬은 감마 카운터로, 혈장 젖산과 암모니아의 측정은 생화학분석기로, 케톤체 측정은 Mass Selective detector로 분석하여 비교 검토한 결과는 다음과 같다. 혈장 알부민농도는 운동전에 비해 운동 직후 운동군, 대조군 모두 유의하게 증가(p<.001)하였고, 혈장 총칼슘은 두 군 모두 유의하게 증가(p<.001)하였으나, 혈장 이온화칼슘농도는 두 군 모두 유의하게 감소(p<.001)하였다. 대사산물인 혈장 젖산(p<.001)과 암모니아(p<.001)가 유의하게 증가되어 pH농도가 유의하게 저하(p<.001)되었다. 한편 부갑상선 호르몬은 두 군 모두 유의하게 증가(p<.001)하였다. 회복기에서 유리칼슘과 케톤체 농도는 5분에, 젖산은 30분에 회복되었으나 다른 변인들은 30분이 되어도 회복되지 못하였다. 이상의 결과에서 일회성 유산소 운동은 트레이닝을 한 사람이나 안 한 사람이나 혈장 유리 이온화 칼슘 농도의 조절은 부갑상선 호르몬과 pH인자 모두가 영향을 주는 것으로 시사되었다. The aim of the present study was to examine how the concentration of blood ionized calcium(Ca++) would change due to pH or parathyroid hormone as a single bout of aerobic exercise. The divided into two groups ; the one is exercise group(n=7), the other is control group(n=7). The exercise group was taken for 30min 60%HRmax aerobic exercise training three times in a week for eight weeks. All subjects were taken 30minute of 60%HRmax on treadmill running, Blood sampling was done pre, post and recovery (5,10 or 30min) from subjects. The plasma albumin concentrations in the both groups were significantly(p<0.001) increased immediately post-exercise compared to pre-exercise. The plasma total Ca concentrations were significantly(p<0.001) increased, while the ionized Ca++ concentrations in the all subjects were significantly(p<0.001) decreased immediately post-exercise compared to the pre-exercise. The lactate(p<0.001) and NH3 (p<0.001) concentrations were significantly in creased, because the pH was significantly(p<0.001) dropped. The plasma PTH concentration was significantly(p<0.001) increased in the exercise group than in the control group. It suggest that the pH and PTH concentration altogether gave to effect the regulation of ionized calcium concentration in a single bout of the aerobic exercise. Further study is needed to clarify the physiological implications of a intensity due to exercise and also it mechanism.

로열코스 Triathlon 경기시 혈장 호르몬과 효소의 반응에 관한 연구

현송자,김원천 대한스포츠의학회 1997 대한스포츠의학회지 Vol.15 No.1

In order to elucidate the interrelationship between pre and post royal course triathlon (1km-swim, 30km-cycle, 9km-run) the investigation was undertaken to determine the enzymatic and hormonal response of plasma. 8 male of physical education students were participated in this study. Our results showed the following significant changes; In the post-triathlon a significant decreased body weight and %body fat were observed, as compared to the pre-triathlon. In the hormones, post-triathlon showed statistically significant increase in aldosterone(p<.01) and adrenaline(p<.05). Although noradrenline and dopamine concentration after race were slightly decreased, there were no significantly difference. Noradrenaline and dopamine concentration showed a moderate but slightly decrease. In the enzymes, the post triathlon race showed all statistically significant increase(p<.01). These result suggest that triathlon changes enzyme and hormone responses, that important factor of aldosterone are probaby involved in the regulation of urinary electrolyte excretions during triathlon. Further studies of triathlete are necessary to identify those phsiological parameter that are of successful triathlon performance.