05 포스터 발표 : 자연생태 환경 분야(PN) ; 식물공장 시스템내 광환경 제어가 적축면 상추 생장에 미치는 영향

허정욱 ( Jeong Wook Heo ),김동억 ( Dong Euk Kim ),이광재 ( Kwang Jae Lee ),강동현 ( Dong Hyeon Kang ),이시영 ( Si Young Lee ),신현만 ( Hyun Man Shin ),허윤선 ( Yoon Sun Huh ),이기열 ( Ki Yeol Lee ),김태중 ( Tae Jung Kim ),김숙종 ( 한국환경농학회 2014 한국환경농학회 학술대회집 Vol.2014 No.-

Plant factory, which considered as a novel plant production system preparing for the climate changes such as a global warming or raining, is an attractive year-round production technique of leafy plants regardless of place or season. The environmental factors of air temperature, relative humidity, nutrient, CO2 concentration, light intensity or quality inside the factory system influencing on growth have been reported. In this study, effects of light-environmental control of quality or intensity were investigated on growth of red curly lettuce ( Brassica rapa) seedlings with different four growth ages (0, 10, 20, and 30 days-old after transplanting) inside the factory system. ‘Tukksum’ red-curly lettuce which developed two true leaves was used as a plant material, and the seedlings were cultured under nutrient solution of 1.5 ds m-1 EC and 5.8 pH for 10 days after light exposure of 50, 100 or 230 μmol m-2 s-1. Light-emitting diodes of Red, blue plus red, and blue plus white were used, and fluorescent light was considered as a control. In 0 day-old seedlings after transplanting, dry weight and number of leaves under blue plus red light of 50 μmol m-2 s-1 light intensity for 10 days increased 28 and 19% comparing with the control, respectively. Increasing of fresh and dry weights in 30 days-old seedlings was significantly stimulated by blue plus red and red light. However, leaf pigmentation was inhibited by the red light regardless of the different growth ages and promoted by the mixture lights. Under 230 μmol m-2 s-1 light intensity, number of leaves, fresh and dry weights were greater by mixture-light exposure on 20 days-old seedlings. On 30 days-old seedlings, increment of dry and fresh weights was also affected by the mixture light. Leaf pigmentation was significantly inhibited by the red light regardless of the different light intensities in all the growth ages. Higher growth and pigmentation could be achieved at 20 and 30 days-old seedlings cultured under the mixture lights of blue, red, or white. It is concluded that light intensity should be controlled according to growth ages for growth and pigmentation, but rather stable control during culture period, and monochromic red is not suitable for leaf pigmentation of red curly lettuce in plant factory system.

온실재배 케일의 생장에 미치는 보광 효과

허정욱 ( Jeong Wook Heo ),김현환 ( Hyeon Hwan Kim ),이광재 ( Kwang Jae Lee ),윤정범 ( Jung Boem Yoon ),이정관 ( Joung Kwan Lee ),허윤선 ( Yoon Sun Huh ),이기열 ( Ki Yeol Lee ) 한국환경농학회 2015 한국환경농학회지 Vol.34 No.1

BACKGROUND: For commercial production of greenhouse crops under shorter day length condition, supplementary radiation has been usually achieved by the artificial light source with higher electric consumption such as high-pressure sodium, metal halide, or incandescent lamps. Light-Emitting Diodes (LEDs) with several characteristics, however, have been considered as a novel light source for plant production. Effects of supplementary lighting provided by the artificial light sources on growth of Kale seedlings during shorter day length were discussed in this experiment. METHODS AND RESULTS: Kale seedlings were grown under greenhouse under the three wave lamps (3 W), sodium lamps (Na), and red LEDs (peak at 630 nm) during six months, and leaf growth was observed at intervals of about 30 days after light exposure for 6 hours per day at sunrise and sunset. Photosynthetic photon flux (PPF) of supplementary red LEDs on the plant canopy was maintained at 0.1 (RL), 0.6 (RM), and 1.2 (RH) μmol/m2/s PPF. PPF in 3 W and Na treatments was measured at 12 μ mol/m2/s. Natural light (NL) was considered as a control. Leaf fresh weight of the seedlings was more than 100% increased under the 3 W, Na and RH treatment compared to natural light considering as a conventional condition. Sugar synthesis in Kale leaves was significantly promoted by the RM or RH treatment. Leaf yield per 3.3 m2 exposed by red LEDs of 1.2 μmol/m2/s PPF was 9% and 16% greater than in 3W or Na with a higher PPF, respectively. CONCLUSION: Growth of the leafy Kale seedlings were significantly affected by the supplementary radiation provided by three wave lamp, sodium lamp, and red LEDs with different light intensities during the shorter day length under greenhouse conditions. From this study, it was suggested that the leaf growth and secondary metabolism of Kale seedlings can be controlled by supplementary radiation using red LEDs of 1.2 μmol/m2/s PPF as well as three wave or sodium lamps in the experiment.

식물공장 시스템내 광환경 제어가 청경채 생장에 미치는 영향

허정욱 ( Jeong Wook Heo ),김동억 ( Dong Eok Kim ),이공인 ( Gong In Lee ),윤정범 ( Jung Beom Yoon ),최규홍 ( Kyung Hong Choi ) 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-

Closed-type plant factory system using artificial light sources instead of sunlight is an ultimated method for plant production without any pesticides comparing to conventional greenhouse structure. Control of the light environment such as a light quality or intensity in the plant factory system using artificial lights is an essential technique for improving the growth and development of plant species. It has been reported that the light environment significantly influences on growth in many kinds of leafy plants cultured under the plant factory system. In this experiment, effects of light quality and intensity under hydroponic culture system were mentioned on growth of green and red Pak Choi (Braccica rapa var. chinensis) seedlings. The Pak Choi seedlings which developed 2∼3 unfolded leaves were cultured in Yamazaki nutrient solution controlled at 1.4 ds m-1 EC and 5.6 pH, respectively on the plant factory of horizontal type. The seedlings were exposed by monochromic or mixture light-emitting diodes (LEDs) of blue+white (BW treatment), red (R treatment), or blue+red+white (BRW treatment) with 50, 100, or 250 μmol m-2 s-1 light intensity for 5 weeks. Increment of fresh and dry weights in the green and red Pak Choi was significantly affected by blue+red+white or red light qualities. However pigmentation was inhibited by the monochromic red light in both of the green and red Pak Choi seedlings. In BRW treatment, fresh weights per seedling of green and red Pak Choi grown under 250 μmol m-2 s-1 light intensity were 5.5 and 8.4 times promoted, respectively compared with fluorescent light considered as a control. Pigmentation in the Pak Choi seedlings increased under BRW treatment with higher light intensity than in lower treatment. From the results, it is suggested that the mixture radiation with higher light intensity gave the maximum growth of Pak choi seedlings with higher quality comparing with monochromic light. Optimum light intensity for leafy plant growth inside the plant factory system using artificial light sources should be decided for commercial production with a lower electric cost.

식물공장 인공광원이 케일의 생육 및 글루코시놀레이트 함량에 미치는 영향

이광재(Guang-Jae Lee),허정욱(Jeong-Wook Heo),정충렬(Chung-Ryul Jung),김현환(Hyun-Hwan Kim),조정수(Jung-Su Jo),이준구(Jun-Gu Lee),이경자(Gyeong-Ja Lee),남상영(Sang-Young Nam),홍의연(Eui-Yon Hong) (사)한국생물환경조절학회 2016 생물환경조절학회지 Vol.25 No.2

본 연구는 식물공장 인공광원이 수경재배 케일의 생육, 수량 및 글루코시놀레이트(GLS) 함량에 미치는 영향을 구명코자 수행하였다. 인공광원으로 LED B:W(1:1, BW), R:B:W(2:1:3, RBW), BW+형광등(1:1+FL, BW+FL) 등 3 처리를 하였다. 수확 엽수와 엽중은 BW+FL이 BW와 RBW보다 우수하였다. 엽장은 BW+FL에서, 엽폭은 RBW가 우수하여 다른 처리와 통계적인 유의성을 나타냈다. 엽록소 함량과 ‘L’ 값은 처리간에 유의성이 없었으며, ‘a’ 값과 ‘b’ 값은 BW+FL에서 가장 낮았다. GLS 함량은 모든 처리에서 glucobrassicin, glucoiberin, gluconasturtiin, sinigrin, progoitrin, glucoraphamin, epiprogoitrin 순으로 많았으며, 총 GLS 함량은 RBW에서 가장 높았다. 잎의 수분 함량, 조단백질, 조지방 함량, 회분 함량은 처리간에 유의성을 나타내지 않았다. 결론적으로 광은 생육과 2차 대사산물의 합성에 차이가 나타내며, 기능성 향상을 위해 후속 연구가 필요하다고 판단된다. This study was carried out to investigate the effects of artificial light sources on growth, yield, and glucosinolate content of hydoroponically grown Peucedanum japonicum in plant factory. Treatments were given with LED Blue:White(1:1, B:W), LED Red:Blue:White(2:1:3, RBW), and LED Blue:White(1:1)+Florescent lamp(BW+FL). Number of harvested leaves and leaf weight of BW+FL were higher than BW and RBW. BW+FL in leaf length and RBW in leaf width were significant difference with other treatments. Chlorophyll content and ‘L’ value were not significant difference among the treatments. The ‘a’ and ‘b’ value is the lowest in BW+FL. Glucosinolate content was high in order of glucobrassicin, glucoiberin, sinigrin, gluconasturtiin, progoitrin, glucoraphamin, and epiprogoitrin in all treatments, and total glucosinolate content was the highest in RBW treatment. Moisture, crude protein, crude fat, and ash content of leaves were not different among the treatments. In conclusion, this study showed that light caused growth and secondary metabolites synthesis, and we recommend to further study between light and secondary metabolites for increasing functionality.

식물공장 재배시스템에 따른 방풍나물의 생육, 비타민 C와 아미노산 함량 및 수량에 미치는 영향

이광재(Guang-Jae Lee),허정욱(Jeong-Wook Heo),정충렬(Chung-Ryul Jung),김현환(Hyun-Hwan Kim),윤정범(Jung-Beom Yoon),김동억(Dong-Eok Kim),남상영(Sang Young Nam) (사)한국생물환경조절학회 2015 생물환경조절학회지 Vol.24 No.4

본 연구는 방풍나물의 인공광 식물공장에서 재배시스템에 따른 생육, 비타민 C, 아미노산함량, 수량에 미치는 영향을 구명코자 실시하였다. 박막수경(NFT), 배지경(Perlite), 분무경(Aeroponics) 등 3처리를 하였다. 초장은 NFT 처리에서 10.2cm 가장 길었으며, Perlite 처리에서 8.9cm로 가장 짧았다. 주당 엽 생체중은 Perlite, NFT, Aeroponics순으로 무거웠다. 총 페놀 함량은 Aeroponics가 117.84mg·100g<SUP>-1</SUP> GE로 가장 높았고, NFT 98.57mg·100g<SUP>-1</SUP>GE, Perlite 74.62mg·100g<SUP>-1</SUP> GE 순으로 재배 방법에 따라 차이를 나타냈다. 총 플라보노이드는 Aeroponics에서만 0.12mg·100g<SUP>-1</SUP>가 검출되었으나, NFT와 Perlite 처리구에서는 검출되지 않았다. 비타민 C 함량은 Aeroponics가 108.23mg·100g<SUP>-1</SUP>으로 가장 높았으며, Perlite가 88.05mg·100g<SUP>-1</SUP>, NFT가 80.83mg·100g<SUP>-1</SUP>으로 처리에 따라 통계적인 유의성을 나타냈다. 총 식이섬유소 함량은 Aeroponics가 Perlite와 NFT보다 높았다. Cystein 함량은 Aeroponics에서 46.76mg ·100g<SUP>-1</SUP>로 가장 높았으며, methionine 함량은 Perlite가 75.64mg·100g<SUP>-1</SUP>로 가장 낮았다. 무기질 함량은 모든 처리에서 K, Ca, P, Mg 순으로 높았다. This study was carried out to investigate the effects of cultural systems on growth, vitamin C, amino acid content, and yield of Peucedanum japonicum grown in artificial light plant factory. Treatments were given with Nutrient Film Technique (NFT), Medium (Perlite), and Aeroponics. Plant height was the highest in NFT system as 10.2cm, and was the shortest in Perlite as 8.9cm. Fresh leaf weight was the high in order of Perlite, NFT, and Aeroponics. Total phenolic compounds was different from cultural systems in order of Aeroponics as 117.84mg·100g<SUP>-1</SUP> GE, NFT as 98.57mg·100g<SUP>-1</SUP> GE and Perlite as 74.62mg·100g<SUP>-1</SUP> GE. Total flavonoid content of Aeroponics is 0.12mg·100g<SUP>-1</SUP> but that of NFT and Perlite treatments is not detected. Vitamin C content in Aeroponics as 108.23mg·100g<SUP>-1</SUP> was significant different from Perlite as 88.05mg·100g<SUP>-1</SUP>as and NFT 80.83mg·100g<SUP>-1</SUP>. Total dietary fiber content was higher Aeroponics than Perlite and NFT. Cystein content was the highest in Aeroponics as 46.76mg·100g<SUP>-1</SUP> and methione content was the lowest in Perlite as 75.64mg·100g<SUP>-1</SUP>. Mineral content of leaves was high in order of K, Ca, P and Mg in all treatments.

태양열원 소규모 해수담수 발생장치 개발

송태빈(Tae Bin Song),허정욱(Jung Wook Heo),전용준(Yong Joon Jun),박경순(Kyung-Soon Park) 대한설비공학회 2016 대한설비공학회 학술발표대회논문집 Vol.2016 No.6

식물공장 인공광원이 방풍나물의 생육 및 수량에 미치는 영향

이광재(Guang-Jae Lee),허정욱(Jeong-Wook Heo),김현환(Hyun-Hwan Kim),정충렬(Chung-Ryul Jung),김동억(Dong-Eok Kim),남상영(Sang-Young Nam) (사)한국생물환경조절학회 2016 생물환경조절학회지 Vol.25 No.1

본 연구는 식물공장 인공광원이 방풍나물의 생육 및 수량에 미치는 영향을 구명코자 수행하였다. 식물공장의 인공광원으로 형광등(FL), LED R:B(2:1, RB), R:B:W(2:1:3, RBW), R:B:G:W(2:1:0.5:3, RBGW) 등 4처리를 사용하였다. 엽중은 RBW와 RB가 FL과 RBGW 보다 우수하였다. 엽장과 엽후는 처리간에 유의성을 나타내지 않았다. 잎의 명도는 초장과 같은 경향을 나타냈다. 총 페놀함량은 RB가 105.77mg·100g<SUP>-1</SUP> GE로 가장 높았고, RBW 92.52mg·100g<SUP>-1</SUP> GE, FL 89.08mg·100g<SUP>-1</SUP> GE, RBGW 82.00mg·100g<SUP>-1</SUP> GE 순으로 높았다. 총 플라보노이드 함량은 모든 처리에서 검출되지 않았다. 비타민 C함량은 RB에서 가장 높았고, FL에서 가장 낮았다. 총 식이섬유소 함량은 FL에서 가장 높았고, RBGW에서 낮았다. Cystein과 methionine의 함량은 처리간에 통계적인 유의성을 나타내지 않았다. 결론적으로 수량, 총 페놀함량, 비타민 C 함량은 RBW와 RB에서 높았다. 수량, 총 페놀 함량, 비타민 C 함량 및 작업자의 눈 피로도 등을 고려 할 때 인공광원으로써 RBW가 가장 적합한 것으로 판단된다. 향후 기능성 성분 증진을 위해 듀티비 등 후속 연구가 필요하다고 판단된다. This study was carried out to investigate the effects of artificial light sources on growth and yield of hydroponically grown Peucedanum japonicum in plant factory. Treatments were composed with; florescent lamp(FL) as control, and LED lights; R:B(2:1, RB), R:B:W(2:1:3, RBW), and R:B:G:W(2:1:0.5:3, RBGW). Plant height of RBGW and FL treatments were superior to RB and RBW. Leaf weight of RBW and RB were superior to FL and RBGW. There were no significant difference of leaf length and thickness among the treatments. Lightness of leaves was same tendency with plant height. Total phenolic compound content was the high in order of RB as 105.77mg·100g<SUP>-1</SUP> GE, RBW as 92.52mg·100g-1 GE, FL as 89.08mg·100g<SUP>-1</SUP> GE, and RBGW 82.00mg·100g<SUP>-1</SUP> GE. Total flavonoids were not detected in all treatments. Vitamin C content was the highest in RB and the lowest in FL. Total dietary fiber were the highest in FL and the lowest in RBGW. There was no significant difference cystein and methionine contents among the treatments. Concludely, yield, total phenolic compounds, and vitamin C content was high in RBW and RB. We reached conclusion that RBW is best artificial light source considering yield, functionality and eye fatigability when work. We recommend to further study LED pulse and duty rates for increasing functionality.

환경제어 조건에서 방충망 색과 크기가 담배가루이 및 꽃노랑총채벌레의 물리적 방제에 미치는 영향

정충렬 ( Chung Ryul Jung ),윤정범 ( Jung Beom Yoon ),김광호 ( Kwang Ho Kim ),이광재 ( Guang Jae Lee ),허정욱 ( Jeong Wook Heo ),김현환 ( Hyun Hwan Kim ) 한국환경농학회 2016 한국환경농학회지 Vol.35 No.1

BACKGROUND: The tobacco whitefly(Bemisia tabaci Gennadius) and western flower thrips(Frankliniella occidentalis Pergande) seriously damaged to several greenhouse crops and transmitted plant viruses such as the Tomato Yellow Leaf Curl Virus(TYLCV) and Tomato Spotted Wilt Virus(TSWV). Objective of this study was to elucidate exclusion effects of insect screen nets by various hole sizes and colors for control of the two insect pests in controlled environments such as a closed plant production system. METHODS AND RESULTS: The exclusion effects to various hole sizes of three other colors with 30 individuals of two insect pests was evaluated. B. tabaci was not showed not difference to different colors and sizes. F. occidentalis showed that 0.2 mm black screen was the most effective exclusion than other colors of 0.6 and 0.8 mm. CONCLUSION: The two insects were different reponses to various hole sizes of white and other color screen nets. It was proved that the 0.4 mm white screen net used in this experimental condition was suitable for exclusion of B. tabaci and 0.2 mm black for F. occidentalis.

광 강도 제어에 따른 LED의 광질에 관한 연구

박상희(Sang-Hee Park),안준철(Jun-Chul An),허정욱(Jung-Wook Heo),최한고(Han-Ko Choi),최성대(Sung-Dae Choi) 한국기계가공학회 2012 한국기계가공학회지 Vol.11 No.6

Light characteristics of the monochromatic red(R), blue(B), green(G) and white(W) and the mixed LED (B-R LED) were investigated by light control a Spectrometer-MMS1 and an illuminometer. The power consumption of each LED was 1W and R LED has five wavelength bands(600nm, 640nm, 660nm, 680nm, 750nm). The light intensity of each LED was changed in a range 10~100%. As a results, the wavelength and the spectrum distribution of R LED increase with increasing light intensity but the wavelength of B, G, W LED decreases. It was found that illumination of each mononochromatic and B-R LED increases linearly with increasing light intensity. It was confirmed that the illumination intensity of R-B light has greater values than those obtained by monochromatic light at the same conditions.

양송이버섯배지의 소형터널에서 후발효 시험

유병기 ( Byeong-kee Yu ),이현동 ( Hyun-dong Lee ),허정욱 ( Jeong-wook Heo ),이승철 ( Seung-chul Lee ),이찬중 ( Chan-jung Lee ) 한국농업기계학회 2020 한국농업기계학회 학술발표논문집 Vol.25 No.2

양송이는 우리나라의 대표적인 식용버섯으로 국내 버섯 총생산량의 8.4%인 11.3천톤이 생산되었다(MAFRA,2018). 배지는 양송이 생산에 필요한 각종 영양분과 수분을 제공하고 있으며, 버섯의 생산량에 결정적인 영향을 미친다. 현재 국내에서는 2·3차 배지 제조를 버섯 재배사에서 수행하고 있어 서유럽의 선진국대비 버섯 재배사 회전율이 절반으로 낮고 재배상의 단위면적당 생산성도 40% 수준이다. 고품질의 양송이 생산을 위하여 국내실정에 맞는 터널발효시스템 개발이 필요하다. 본 연구에서는 우리나라 실정에 맞는 소형 터널시스템 개발을 위하여 시험장치를 제작하고 후발효(Phase 2)시험을 수행하였다. 1차 배지 주재료는 밀짚, 볏짚, 폐면이 4:4:2의 건물중량비로 섞고 계분을 섞어서 굴삭기로 야외발표한 배지(Phase 1)를 사용하였으며, 시험용 소형터널은 2,500×2,500×5,500 mm(W×H×L) 규격의 내부용적과 100mm두께의 우레탄 센드위치 판넬로 단열하였다. 호기상태 유지와 냉각을 조절하기 위하여 선행연구에 따라 톤당 최대 200CMH 까지 확보하기 위한 최대정압 380mmAq, 최대 풍량 3,780CMH 원심형터보송풍팬을 인버터로 프로그램에 따라 조절하도록 제작하였다. 터널에 의한 후발효(Phase 2) 공정은 1.온도평탄화작업 2.온도를 60℃까지 올리는 가온작업 3.저온살균작업(56~60℃, 8시간), 4.온도를 49℃ 이하로 낮추는 하온작업, 5.온도를 46~49℃로 유지하면서 암모니아 가스가 5ppm이하로 줄어들게 하는 컨디셔닝 공정 6.암모니아가 줄어들면 종균 접종을 위하여 온도를 25℃로 낮추어 배출하는 단계로 구성된다. 위의 6단계가 외기를 불어 넣는 팬에 의해서만 조절되고 가온은 발효열에 의한다. 스마트폰과 인터넷 PC로 온도를 모니터링하거나 팬등을 제어할 수 있도록 구성하였다. 시험결과 5단계인 컨디셔닝 단계에서 최대 풍속으로 공기를 공급하여도 온도 유지가 안 되는 현상이 나타났는데 이는 배지 주재료로 밀짚만 사용하는 유럽과는 달리 볏짚과 폐면을 섞어 쓴 것 때문으로 판단되어 밀짚만을 주재료로 추가 시험이 필요한 것으로 판단되었다.