체외수정시술주기에서 배아와 난구세포의 공배양 효과에 관한 연구

허의종,이원기,Hur, Eui-Jong,Lee, Won-Ki 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3

Despite the rapid development of assisted reproductive technologies (ART) in recent years, implantation rates after replacement of embryos into the uterine cavity remains low. Several techniques such as culture conditions based on formulations of human tubal fluid and various ART techniques as GIFT, ZIFT, TET have been adopted in recent years to improve embryo viability in vitro and implantation rates. Also, coculture of human IVF-derived embryos have been used in an effort to increase the number of viable embryos following IVF and to improve synchrony between the developing embryo and the uterine environment. The aim of this study was to evaluate whether the use of co culture with autologous cumulus cells has a significant beneficial effect on the development of embryos in vitro and its relation to the pregnancy rates in 120 patients with previous failed IVF-ET from September, 1995 to January 1998. We obtained the results from which significant improvement in the quality of viable embryos were observed using a coculture system with autologous cumulus cells, but pregnancy rates in this group of patients did not differ from the rate in the standard IVF group during the same period. Our study shows that a simplified short-term coculture system with autologous cumulus cells may help rescue moderate quality embryos to cleave regularly.

A Clinical Experience in use of Monalazone Disodium(Speton(R)) for Spermicidal Activity

허의종 ( Eui Jong Hur ),박형무 ( Hyoung Moo Park ),김우균 ( Woo Kyoon Kim ),배도환 ( Do Hwan Bae ) 대한산부인과학회 1987 대한산부인과학회 학술대회 Vol.60 No.-

Methotrexate의 국소적ㆍ전신적 요법으로 치유한 자궁경관임신

허의종(Eui Jong Hur),박진완(Jin Wan Park),이원기(Won Ki Lee),하진완(Jin Wan Hah) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.12

Cervical pregnancy is a rare form of ectopic pregnancy and it can cause life-threatening bleeding. Until recently abdominal hysterectomy has been considered the standard therapy for fear of profuse hemorrhage. Methotrexate, however, can be useful agent to conserve further fertility and it is used in various routes of administration. We report a case of cervical pregnancy that was successfully treated by intraamniotic methotrexate injection under the guidance of transvaginal ultrasonography and subsequent four systemic doses thereafter.

비파열성 자궁외임신에서 Methotrexate 의 전신투여요법과 국소투여요법의 비교

허의종(Eui Jong Hur),박진완(Jin Wan Park),최용욱(Yong Wook Choi) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.1

N/A Objective : This study was performed to evaluate the efficacy of treating of unruptured ectopic pregnancy by systemic and transvaginal local administration of methotrexate. Methods : We reviewed the medical records of 32 patients who were treated with methotrexate from June 1995 to October 1999. Among the patients, 14 were treated by ultrasound-guided transvaginal local injection and 18 were treated by systemic intramuscular administration. Success rate of the treatment was examined for each group, and the cases of methotrexate failure were characterized and compared with the success group. Results : The success rate was similar between the transvaginal (85.7%) and the systemic (77.8%) groups. There were no differences in initial serum hCG levels, size of gestational sac, fetal cardiac activity between the success group and failure group. Conclusion : The results of this study suggest that there is no difference in efficacy between systemic and transvaginal local methotrexate administration, and there are no useful parameters in identifying the risk for the failure of methotrexate treatment.

A Clinical Evaluation of Tioconazole (Gyno-Trosyd(R)) as a Single Dose therapy for vaginal Candidiasis

허의종 ( Eui Jong Hur ),박형무 ( Hyoung Moo Park ),김우균 ( Woo Kyoon Kim ),배도환 ( Do Hwan Bae ) 대한산부인과학회 1987 대한산부인과학회 학술대회 Vol.60 No.-

임신중 발생한 포진상 농가진

김종수 ( Jong Soo Kim ),허의종 ( Eui Jong Hur ),박진완 ( Jin Wan Park ),신용우 ( Yong Woo Shin ),이원기 ( Won Ki Lee ) 대한주산의학회 1999 Perinatology Vol.10 No.1

근치적 자궁절제술을 받은 자궁경부암 환자에서 고위험 예후인자 존재유무, 임상병기, 그리고 병리학적 유형에 따른 E-cadherin 발현양상에 관한 연구

이민희(Min Heui Yi),허의종(Eui Jong Hur),박진완(Jin Wan Park),이민철(Min Chul Lee),이원기(Won Ki Lee) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.6

목적 : 자궁경부암에서 E-cadherin의 발현정도와 고위험 예후인자 (림프절 전이 또는 자궁방 전이), 임상병기 그리고 병리학적 유형과의 상관관계가 있는지를 알아보기 위해 본 연구를 시행하였다. 연구방법 : 근치적 자궁절제술만 시행받은 20명과 고위험 예후인자가 있어 근치적 자궁 절제술 후 항암화학요법이나 방사선치료를 시행받은 16명을 대상으로 포르말린 고정 파라핀 포매 조직을 이용하여 E-cadherin 면역조직화학검사를 시행하였다. 각 항목별 빈도의 차이는 카이제곱검정법 (chi-square test) 으로 분석하였으며, 검정결과는 p-value 가 0.05 미만인 경우를 통계학적으로 유의하다고 판정하였다. 결과 : 고위험 예후인자 (림프절 전이 또는 자궁방 전이) 유무에 따른 E-cadherin 발현정도는 통계학적으로 유의하지 않았으며 , 임상병기 그리고 병리학적 유형에 따른 E-cadherin 발현정도도 통계학적으로 유의하지 않았다. 결론 : 침윤성 자궁경부암에서 E-cadherin 발현정도는 고위험 예후인자, 임상병기, 그리고 병리학적 유형에 관계가 없었다. Objective : To analyze the relationship between the degree of expression of E-cadherin and presence of high risk prognostic factors (lymph node metastasis or parametrium involvement), clinical stages and pathologic types in invasive cervical cancer.Methods : An immunohistochemical technique has been applied to formalin-fixed, paraffin- embedded samples from 20 radical hysterectomy without risk factors and 16 radical hysterectomy with risk factors. The degree of expressions of E-cadherin immunostaining was compared with the International Federation of Gynecology and Obstetrics (FIGO) stage, presence of high risk prognostic factors, and pathologic types.Results : The difference of the degree of expression of E-cadherin was not statistically significant between high-risk group (lymph node metastasis or parametrium invasion) and non-risk group. The difference of the degree of expression of E-cadherin was not statistically significant according to clinical stages and variable pathologic types either.Conclusion : These results suggest that the degree of the expression of E-cadherin has no relationship with known high risk prognostic factors, clinical stages and pathologic types in invasive cervical cancer.

항갑상선 항체 양성율과 산후 갑상선염의 연관성에 관한 연구

신광철 ( Kwang Cheol Shin ),허의종 ( Eui Jong Hur ),고경심 ( Kyung Sim Koh ),박진완 ( Jin Wan Park ),이원기 ( Won Ki Lee ) 대한주산의학회 1998 大韓周産醫學會雜誌 Vol.9 No.3

폐경여성에서의 승마추출물의 치료효과

박진완(Jin Wan Park),허의종(Eui Jong Hur),이원기(Won Ki Lee) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.1

N/A Objective : The aim of the study is to assess the effects of treatment with an extract from Cimicifuga racemosa for 1 year in postmenopausal women with symptoms. Methods : In a randomised, open-label, group-comparative study, the change on the Kupperman menopausal index, serum FSH and E2 level, bone densitometry (BMD), and mammographic density by an extract from Cimicifuga racemosa (GYNO-Qxⓡ) (N=40) were compared with an conjugated equine estrogen combined with medroxyprogesterone acetate (CEE/MPA) (N=36) during 12 months in menopausal women. Results : There were no statistical differences in the change of Kupperman index, serum FSH and E2 level, and mammographic density between two groups after 12 months, except the effect of BMD that the CEE/MPA group (+6.6%) was significantly increased as compared to the extract from Cimicifuga racemosa (+1.1%) baseline. Conclusion : This study suggests that an extract of Cimicifuga racemosa is a safe, effective alternative to estrogen replacement therapy for patients in whom hormone replacement therapy is either refused or contraindicated.

인간난관상피세포 및 Vero 세포가 생쥐배아의 발생에 미치는 영향에 대한 연구

김수곤(Su Kon Kim),박진완(Jin Wan Park),허의종(Eui Jong Hur) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.6

목적 : 본 연구는 사람의 난관세포와 Vero 세포를 각각 생쥐의 배아와 공배양함으로써 사람의 난관세포와 Vero 세포의 공배양이 배아발생에 미치는 영향에 대해 비교해 보고자 한다. 연구 방법 : 자궁절제술을 받은 가임기 여성의 난관에서 채취된 난관상피세포와 Vero 세포를 배양하여 준비한 후, 생쥐에서 얻은 배아를 FBS (Fetal Bovine Serum)을 첨가한 Ham`s F-10 배양액에서 공배양하여 배아의 발달 단계별로 관찰하고 Ham`s F-10에 10% FBS가 첨가된 배양액에서 세포없이 배아 단독으로 배양한 군을 대조군으로 하여 비교 분석하였다. 결과 : 1. 난관상피세포 및 Vero 세포와 공배양한 군에서 배아 발생율은 각각 81%와 79.5%로 나타났으며, 대조군의 65.5%에 비하여 유의하게 높게 나타났다 (p<0.05). 난관상피세포 및 Vero 세포와 공배양한 군에서 배아의 부화율 (hatching rate)도 각각 63.5%와 60.5%로 대조군의 34.5%에 비해 유의하게 높게 나타났다 (p<0.05). 2. 계대 배양된 난관상피세포와 생쥐배아를 공배양하였을 때 계대 배양된 상피세포와의 공배양시 발생율과 부화율은 각각의 계대간 상피세포와의 공배양이 배아의 발생에는 유의하게 작용하지 않았다. 또한 계대 배양된 Vero 세포와의 공배양시 발생율과 부화율은 각각 83.3%와 63.3%로 계대 배양된 상피세포와의 계대 배양된 Vero 세포와의 비교에서도 통계학적으로 유의하지 않았다. 3. 동결-해빙한 공배양 세포와 공배양시 배아의 발생율을 살펴보면 난관상피세포와 공배양할 경우 계대 배양시 발생율과 부화율은 동결-해빙후 계대 배양한 공배양군에서의 발생율 및 부화율과 유의한 차이는 나타나지 않았다. 또한 동결-해빙후 배양된 Vero 세포와의 공배양에서는 발생율과 부화율은 각각 81.3%와 67.3%로 동결-해빙후 계대된 상피세포와의 비교에서도 통계학적으로 유의하지 않았다. 4. 계대 배양된 난관상피세포와 동결-해빙된 난관상피세포에서 공배양시 각각의 발생율과 부화율은 유의한 차이는 나타나지 않았다. 또한 계대 배양한 Vero 세포와 동결-해빙된 Vero 세포에서 공배양시 각각에서 발생율과 부화율은 유의한 차이가 나타나지 않았다. 결론 : 체외수정 시술에서 사람의 난관세포 및 Vero 세포와의 공배양은 생쥐배아의 발달 및 부화에 증진 효과가 있음을 확인할 수 있다. 또한 난관상피세포 및 Vero 세포와의 공배양이 생쥐배아의 발생율과 부화율에는 유의한 차이가 없음을 관찰할 수 있으며 공배양세포를 동결-해빙하여도 그 기능에는 큰 차이가 없음을 관찰 할 수 있다. 따라서 체외수정 프로그램시 난관상피세포나 Vero 세포를 사용하여 공배양하면 임신성공율 향상에 좋은 결과를 가져올 것으로 생각된다. Objective : The purpose of the study was to determine the effects of co-culture with oviductal epithelial cells and Vero cells on mouse embryo. Method : For the control group, mouse embryos were cultured alone in Ham`s F-10 with 10% FBS. Subcultured oviductal epithelial cell and Vero cell were cocultured in Ham`s F-10 with 10% FBS with the mouse embryo and used as the treatment group. Development of mouse embryos were observed. Result : The development rate and hatching rate of embryos that cocultured with oviductal epithelial cell and Vero cell was significantly higher (p<0.05) than control group. When subcultured oviductal epithelial cells were co-cultured with mouse embryo, there was no significant difference in development rate and hatching rate among subculture step. When oviductal epithelial cells that have been frozen-thawed were co-cultured with mouse embryo, there was no significant difference in development rate and hatching rate among subculture step. No statistical significance was seen in the development rate and hatching rate between subcultured oviductal epithelial cells and frozen-thawed oviductal epithelial cells when cocultured with mouse embryo, Vero cells and frozen-thawed when cocultured with mouse embryo, and Vero cells and oviductal epithelial cells when cocultured with mouse embryo. Conclusion : Oviductal epithelial cells and Vero cell may have a stimulatory role in early mouse embryonal development compared to control in vitro. As well, there is no significant difference in development rate and hatching rate among subculture step, when early mouse embryo was cocultured with cells that subcultured and frozen-thawed.