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한혜림,정영진,신상철 한국산림과학회 2008 Forest Science And Technology Vol.4 No.2
Pine wood nematode (PWN), Bursaphelenchus xylophilus, is a causal organism to induce pine wilt disease (PWD) in many varieties of pine trees. PWD was first introduced to Korea in 1988 but, the damage has been dramatically increased since 2000. Recently PWD is newly reported in Korean pine tree (Pinus koraiensis) and is considered one of the most important forest diseases in Korea. Fifteen isolates of B. xylophilus, 2 isolates of B. mucronatus, and 6 unidentified Bursaphelenchus spp. were collected from different geographical locations and hosts in Korea. All the isolates were cultured in fungal medium and nematodes were harvested for further experiment. First, all the isolates were characterized by both ITS and D2D3 rDNA sequence analysis. Template DNA was prepared by DNA extraction from single female nematode. ITS and D2D3 region were amplified by PCR and followed by cloning and sequence. As a result, all the sequences of ITS and D2D3 from B. xyophilus isolates were identical and there is no intraspecific variation. However, 2 genotypes of B. mucronatus were found, which one from P. thunbergii was East Asian type and the other one from P. koraiensis was European type. ITS-RFLP phenotype was also proposed to discriminate different species and genotypes and 5 enzymes, Hinf I, Alu I, Msp I, Hae III, and Rsa I. were selectively treated.
The Tribological Properties of Alkanediol Derivatives Containing Dialkyldithiophosphoric acid
한혜림,심대선,조정은,강충호,김영운,정노희,강호철 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
Zinc dialkyldithiophosphate(ZDDP)는 엔진 오일의 첨가제로써 산화 방지, 마모 억제 등의 성능과 저렴한 가격으로 인하여 오랫동안 사용되어져 왔다. 이러한 ZDDP의 성능 중 마모 억제를 목적으로 하며, zinc와 같은 금속을 포함하지 않고 dialkyldithiophosphoric acid(DDP)를 포함하는 알칸 디올 유도체를 효율적으로 합성하고, 이에 따른 마찰학적 특성에 대하여 연구하였다. Butanediol을 사용하여 에스테르화하여 bis[methacryloyloxy] butane을 합성한 후, DDP의 알킬 사슬에 변화를 주어 bis[3-(dialkyloxyphosphorothionyl)thio-2-methyl propanyloxy] butane을 합성하고 이에 따른 내 마모 성능을 살펴보았다. 알킬 사슬의 탄소 수가 4, 8, 10일때, 각각의 WSD는 0.595, 0.459, 0.494 mm로 측정되었다. DDP의 알킬 사슬의 탄소수가 4개일 경우 WSD값이 가장 크게 측정되었고, 탄소수가 8개일 경우 가장 적은 값으로 측정되었다. 탄소수가 8개 이상일 경우, WSD값이 서서히 증가하는 것으로 관찰되었다.
국화재배지의 식물기생선충 분포조사 및 뿌리썩이선층의 ITS와 D3-28S rDNA 특성조사
한혜림,이재국,최동로,한만종,박병용,Han, Hye-Rim,Lee, Jae-Kook,Choi, Dong-Ro,Han, Man-Jong,Park, Byeong-Yong 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.3
2005년 5월부터 6월까지 국내 주요 국화재배 포장에서 총 50개 토양시료를 채집하여 식물기생선충상을 조사하였다. 조사 결과, 뿌리썩이선충(Pratylenchus)의 포장발생률이 86%로 대부분의 포장에서 광범위하게 분포하였고, 밀도는 토양 200 cc와 뿌리 1g에서 평균 1,095마리가 검출되었다. 분자생물학적 동정을 위하여, '무안', '구미', '태안', '정읍', '마산' 등 5지역을 선정하고, 그 지역의 뿌리썩이선충을 대상으로 ITS와 D3-28S rDNA의 유전자 분석을 하였다. PCR 결과 증폭된 ITS는 '무안'의 경우 1 kb 크기로 나타났고, 그 외의 다른 지역의 뿌리썩이선충에서는 0.8 kb로서 약 200 bp가량의 차이가 있었다. D3-28S rDNA의 PCR 결과에서는 모든 지역에서 약 320 bp로 일정한 크기의 gene이 증폭되었으며, 증폭된 DNA는 sequencer를 이용하여 염기서열정보를 얻었다. 얻어진 각 isolate의 D3 염기서열 정보는 DNASTAR 분석 소프트웨어의 MegAlign 프로그램을 이용하여 분석 및 phylogenetic tree를 형성하였다. 분석 결과 '구미'와 '태안' isolate의 D3 염기서열은 100% 일치하였고, '정읍' isolate는 이들 isolate와 99.7%의 유사성을 나타내었다. 또한 이들 세 isolate는 Pratylenchus vulnus와 근연종임이 확인되었는데, '구미', '태안'은 96.7%, '정읍'은 96.3%의 유사성을 각 나타내었다. 한편, '마산' isolate는 P. penetrans와 100% 일치됨을 나타내었고, '무안' isolate는 P. brachyurus와 99.7%의 유사성을 나타냄으로써 높은 상관성을 나타내었다. 이와 같은 결과는 phylogenetic tree에서도 동일한 결과를 나타내고 있다. A survey was conducted to find out the major plant parasitic nematode in Chrysanthemum morifolium fields in Korea from May to June in 2005. A genus of Pratylenchus was determined as the most important plant parasitic nematode based on analysis of total 50 samples from 8 cities of chrysanthemum field. Pratylenchus showed 86% occurrence rate and average numbered 1,095 per 200cc soils and 1g root. Five Pratylenchus isolates, 'Muan', 'Masan', 'Tean', 'Gumi', 'Jeongup', were selected for the molecular identification of the species of Pratylenchus, and ITS and D3-28S ribosomal DNA were amplified by PCR. For the ITS, only 'Muan' isolate was differentiated by total 1 kb PCR amplification, which was 200 bp larger than all the other isolates. There was no size variation in amplified D3-28S rDNA and all isolate represented approximately 320 bp of PCR product. Sequence data of D3-28S rDNA were analysed by MegAlign program in DNASTAR software and phylogenetic tree was constructed. Sequence homology was 100% between 'Gumi' isolate and 'Tean' isolate and 'Jeongup' isolate was also close to these isolates by 99.7% sequence homology. 'Gumi', 'Tean' group and 'Jeongup' isolate were determined to be closely related to Pratylenchus vulnus by 96.7% and 96.3% similarity in respectively. D3 sequence of 'Masan' isolate was 100% identical to P. penetrans, and 'Muan' isolate showed 99.7% similarity to P. brachyurus. This result was congruent with the branch divergence pattern shown in phylogenetic tree.
성인애착과 친밀감에 대한 두려움 간의 관계: 자아분화와 자아존중감의 매개효과
한혜림,이지민 대한가정학회 2022 Human Ecology Research(HER) Vol.60 No.3
This study analyzed the relationship between adult attachment, self-differentiation, and self-esteem in solving the fear of intimacy in interpersonal relationships experienced by adults. This study targeted people in the 20s to 50s age group, and data from 1,263 participants were analyzed using SPSS 23.0 and AMOS 23.0. The results are as follows. First, it was found that secure attachment had a negative relationship with the fear of intimacy, while the relationship between self-differentiation and self-esteem was positive. On the other hand, anxiety attachment and avoidance attachment were shown to have a positive relationship with the fear of intimacy, but a negative relationship was identified with self-differentiation and self-esteem. Second, it became evident that self-differentiation and self-esteem mediated the relationship between adult attachment and the fear of intimacy. Additionally, the findings indicated that self-differentiation and self-esteem had a dual mediating effect. The results of this study can be used as basic data for counseling and educational programs to solve the fear of intimacy in interpersonal relationships experienced by adults.