아이들 때문에 울고 아이들 때문에 웃는다

하은숙 예수살이공동체 2002 산위의 마을 Vol.5 No.-

오가피로부터 분리된 조다당 분획물의 면역자극활성 및 Cisplatin과의 병용에 의한 항암 상승작용의 유도

하은숙,황수현,유광원,신광순,조형민,김창한,박우문,윤택준 대한약학회 2003 약학회지 Vol.47 No.3

In order to study the clinical usefulness of crude polysaccharides fractionated from Eleutherococcus senticosus, EN-3, in eliminating tumors, we have investigated the effect of combination therapy on the murine tumor metastasis and growth models. In experimental metastasis of colon26-M3.1 cells, prophylactic intravenous (i.v.) administration of EN-3 (0.5, 5, and 50 $\mu\textrm{g}$/mouse) inhibited tumor metastasis compared with tumor control group in 33.6, 66.8, and 81.8% respectively. The administration of EN-3 (50 $\mu\textrm{g}$/mouse) also exhibited a 66.1% therapeutic effect on lung tumor metastasis. Although EN-3 induced no toxic effect on both tumor cell and normal splenocyte in the concentration below 100 $\mu\textrm{g}$/mι in in vitro, it induced significant proliferating activity on normal splenocyte in the concentration-dependent manner. In an analysis of NK-cell activity, i.v. administration of EN-3 (4∼100 $\mu\textrm{g}$/mouse) significantly augmented NK cytotoxicity to YAC-1 tumor cells. The combination treatments of cisplatin (10 $\mu\textrm{g}$) and EN-3 (5 $\mu\textrm{g}$) induced synergistic effect on the inhibition of tumor metastasis in experimental tumor metastasis model produced by colon26-M3.1 cells. In addition, the combination treatments also exhibited prolongation of lifespan in S∼180 tumor bearing mouse for over the 60 days. Even though cisplatin (2.5 $\mu\textrm{g}$/mι) exhibited cytotoxicity to tumor cells and inhibited tumor growth over 95% in in vitro, combination treatment with EN-3 (20 $\mu\textrm{g}$/mι) was induced splenocyte proliferation and produced cytokines, such as TNF-$\alpha$, IL-1 and IL-12, from the macrophages. These results suggested that EN-3 stimulate immune system non-specifically and apply to the biological response modifiers (BRM) in chemo-immunotherapy for tumor prevention.

소와 및 열구의 인공 초기 법랑질 우식병소에 대한 불소유리 전색재의 영향

하은숙,김신 大韓小兒齒科學會 1993 大韓小兒齒科學會誌 Vol.20 No.1

With the view of comparing the effects of pit and fissure sealant of conventional and F-releasing type upon early fissure caries, an experimental study was performed. The sealants of both types were applied upon caries-induced enamel surface respectively, and their remineralizing effects were evaluated. On the other hand, early enamel caries was induced artificially at sealed occlusal surfaces with sealants of both types. Specimens were observed under polarized light microscope, and lesion depths of each group were measured. The obtained results were as follows: 1.There showed classical features of negative refringence at surface zone and positive refringence at subsurface zone in all specimens under polarixed light. 2.Comparing the lesion depths under the sealed areas with sealants of both types after caries induction,lesion depths of F-releasing sealant group(69.6± 12.5㎛) were shallower than those of conventional sealant group(100.7±20.6㎛) (P<0.01). Surface zone was more remarkable in F-releasing sealant group. 3.Comparing the lesion depths around the sealed areas after cariesinduction over sealed areas with sealants of both types, lesion depths of F-releasing sealant group(75.8±21.9㎛)were shallower than those of conventional sealant group(139.2±18.4㎛) (P<0.01).

가시오가피로부터 분리한 단백 다당물질의 경쟁적 ELISA법에 의한 분석

하은숙(Eun Suk Ha),황수현(Soo Hyun Hwang),신광순(Kwang-Soon Shin),유광원(Kwang-Won Yu),이경호(Keyung Ho Lee),최주선(Joo Sun Choi),박우문(Woo-Mun Park),윤택준(Taek Joon Yoon) 한국식품과학회 2003 한국식품과학회지 Vol.35 No.6

Methylene Chloride Fraction of Spatholobi Caulis Induces Apoptosis via Caspase Dependent Pathway in U937 Cells

HA, Eun-Suk,LEE, Eun-Ok,YOON, Taek-Joon,KIM, Jin-Hyung,PARK, Jong-Oh,LIM, Nak-Cheol,JUNG, Sung-Ki,YOON, Byung-Soo,KIM, Sung-Hoon WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Spatholobi Caulis has been used in Oriental medicine to treat cancer and blood stasis. In this study, the methylene chloride fraction of Spatholobi Caulis (MCSC) was examined to determine if it possesses anti-cancer activity via its apoptosis-inducing activity. MCSC exhibited a strong cytotoxic effect against human monocyte leukemia U937 cells (IC_(50)=15.1 μg/ml). A TUNEL assay showed that the MCSC caused a characteristic ladder pattern of discontinuous DNA fragments and apoptotic bodies. Flow cytometric analysis confirmed that MCSC significantly increases the number of apoptotic cells stained by annexin V^(+)/PI^(-) cells. Western blotting revealed that MCSC activated caspase-3 expression and cleaved poly (ADP-ribose) polymerase (PARP) in a concentration-dependent manner. An enzyme-linked immunosorbent assay (ELISA) demonstrated that MCSC significantly activated the caspase-3 activity compared with the untreated control by. Taken together, these results suggest that MCSC can induce apoptosis in U937cells via the caspase dependent pathway.

淸州工業團地의 發展過程과 構造的 特性에 관한 硏究 : A Study on the Development Proceses and the Structural Characteristics of Chongju Industrial Complex

南基昶,河銀淑 청주대학교 새마을연구소 1991 새마을硏究論文集 Vol.8 No.-

Chongju is the industrialized city, which is located near the industrialized country much more. Until 1950s the industry of Chongju didn't escape from the degenerated situation. But since 1960s its industrial structure has been developed rapidly and gradually because of economical growth. In recent days, according to the fundamental project of rearranging Metropolitan areas, Chongju has acted the faculty of Metropolitan area and industry, and it has been suited for the place which can scatter populations. And then the improving project of local industries in 1972 has changed Chongju country. Also Chongju is the place which can be developed in maximum in that Chongju has much water for industry, the price of land is cheap and it has been exempted from the various taxes. From the point of View like above elements, I scrutinized the developement processes and structural characteristices of Chongju Industrial Comples on the ground of the followings : 1. Development Processes (1) Since the Industrial Complex had been established in 1972, the industries and factories in Chongju Industrial Comples are 110. Among them 87 industries are showing the working ratio of 79 percents. And the female workers are much more than the male workers. This means that the industries and factories which are making clothes, leather products, and electronic products, and so on are needing the female workers. (2) Viewing the development processes of Chongju Industrial Complex as the first, the second, and third period in order, the industries and factories which making metal products, clothing products, leather products, beverages, foods, and tabbaco are much more than other industries. And the number of workers is much more than other industries. In the scale of industries, most of them are minor enterprises. In the second and third period, most of all are minor enterprises except for the industries which making Fabricated metal products and machinery, chemicals, pertroleum, coal, rubber, and plastic products. Especially the industries and factories of chemicals, petroleum, coal, rubber, and plastic are more established than the second and third period. And most of them are bigger industries and factories than minor enterprises. This shows the fact that the big enterprises of chemicals, petroleum, coal, rubber, and plastic moved into the Chongju Industrial Comples from other counties. 2. Structural Characteristics (1) The structure of industrial classifications a. The enterprises of chemicals and petroleum, coal, rubber, and plastic take the large part of industrial classifications. And next the one of fabricated metal products, machinery and equipment takes the large part in industrial classifications. This show the fact that two types take an important state. b. The number of workers in the industries of fabricated metal products and machinery, equipment take an overwhelming ratio. And next is the industries of chemicals and petroleum, coal, rubber, and plastic. And third is the industries of the clothes and leather products. The number of workers are Niger than the average ratio than the one of the whole country. From this point of view, we can recoginize that the manufacturing industry of Chongju is very different from other countries. c. From the point of productivity, the industries of chemicals and petroleum, coal, rubber, and plastic are high. And next is the part of fabricated metal and equipment products. Comparing this situation to the whole country, the industries of chemicals and petroleum, coal, rubber, and plastic have the higher productivity than others though the number of industries is fewer than others. But the productivity of ther industries is lower than other counties except for the two types. d. The factories of interior possession are much more than the exterior possession. Especially the industries of chemicals and petroleum, coal, rubber, and plastic products are much more than the interior possession. And than main offices of exterior possession's factories are much more in Seoul than other countries. This shows the fact that the factories of exterior possession have close connections with Seoul to accomplish the various activities on the ground good conditions of location and cheap working forces in Chongju. For example the acts of selling, understanding of market condition, and collecting of informations and so on are included in the various activities. (2) The structure of scaling a. In the number of the factories, the factories of workers, 100-299 are large, and the factories of workers, below 500 are much more. And seeing the fact that the factories of workers, over 1,000 are lower, we can recognize that most of the factories are minor enterprises. The industries which having workers, over 1,000 are the factories of fabricated metal products, machinery, equipment, and the others are the factories of chemicals and petroleum, coal, rubber, and plastic. This shows the structure of laying disproportionate emphasis on the Chongju Industrial Complex. b. In the number of workers, the biggest scale is the case of workers, 1,500~2,499. And the smallest case is two cases ; workers, 0~49 and workers 300~499. The factories which having workers, over 1,000 are much fewer than the number of workers. This shows the faot that most of workers are engaging in big enterprieses. Also most of workers are engaging in the big scale factories which are of chemicals, petroleum, ccoal, rubber, and plastic, and then fabricated metal products, machinery, and equipment. We can recognize that there is a gap between the factories of workers, 1,000 and 500. The most of the factories in Chongju Industrial Complex are minor enterprises who engaging in minor enterprises is higer than other countries. Then are the factories which making clothes, leather products, fabricated metal products, machinery, and equipment. This means the fact that Chongju is the small area and has a small amount of capital as local city. 3. In scrutinizing Chongju Industrial Complex, I cannot grasp dearly the benefit of each factory, import and export, and the information on importing and exporting countries with including the hometown, education, and sex of workers because of lacking data. I think that the aid of the cith administration, the managing agency of Chongju Industry, and Ministry of commerce & Industry is necessary for this part. 4. Because there is not a concrete criterion to study Chongju city, the colse relationship between the managing agency of Chongju Industrial Complex and the administration of Chongju City is necessary. It is the reason why Geography has to treat the influence of industry toward a community and vice versa. Also I cannot very well grasp the characteristic structure of Chongju Industrial Comples because there has not been the informations of the social and economical characteristions of employees who has been alenated from societies and enterprises. And then I cannot grasp completly the employees way of thinking and the improvement of management because of lacking data. So I hope that the administration of Chongju city and the managing agency of Chongju Industrial Complex shall have a special interest on this sphere. There fore these facts will be the objects which can be studied later.

Vygotsky의 ZPD이론에 기초한 유아 교수-학습방법에의 적용

이현섭,하은숙 진주산업대학교 2001 論文集 Vol.40 No.-

The purpose of this study are to investigate Vygotsky's Zone of Proximal Development (ZPD) theory, and to discuss his theory in terms of the practice of early childhood education. Based on Vygotsky's theory of ZPD, the following suggestions can be drawn : 1. In order to provide the foundation of learning of young children, teachers should evaluate them using dynamic method of assessing their ZPD. 2. Since teacher are one of the most important factors influencing children's development, they should try to improve their professional quality. 3. The ratio of teacher vs children should be lower and children should be divided into small groups for more effective scaffolding. 4. Mixed age group is more effective than homogeneous age group in order to enhance young children's active peer interaction. 5. Teacher should select and provide children with various activities in which children can experience cooperation with peers and social interaction with other using language. 6. Teacher should establish an appropriate educational objectives for each child on the basis of the results of the dynamic assessment

오가피 조다당의 단백질 항원 (BSA and OVA)에 대한 면역증강효과

황수현,하은숙,유광원,신광순,이상훈,이재권,이경호,윤택준,박우문 대한약학회 2003 약학회지 Vol.47 No.3

Eleutherococcus senticosus is a typical oriental folk medicinal herb. It has been used clinically as a anti-rheumatic disease, anti-stress, ischemic heart disease and gastric ulcer. In the present study, we examined the adjuvant activity of the crude polysaccharides fraction from Eleutherococcus senticosus, EN-3, on the induction of humoral and cellular immune responses against bovine serum albumin (BSA) or ovalbumin (OVA). The thioglycollate-induced macrophages and silica-induced dendritic-like cells cultured with BSA and EN-3 synergistically increased the production of TNF-$\alpha$ and IL-12. When mice were subcutaneously immunized with BSA + EN-3, the antibody titer against BSA was showed significantly higher than those immunized with BSA alone. In addition, when mice were immunized with OVA + FIA + EN-3, the antibody titer was showed similar patterns with the FCA. The assay for determining subisotype of antibody revealed that EN-3 augmented OVA-specific antibody titer of IgG1 and IgG2b. The culture supernatant obtained from splenocytes of mice treated with OVA + FIA + EN-3 also showed a higher level of both OVA-specific Th1-type (IL-2, IFN-${\gamma}$ and GM-CSF) and Th2-type cytokine (IL-4, IL-6 and IL-10). In vitro analysis of T cell proliferation to OVA on 8 weeks, the splenocytes of mice treated with OVA + EN-3 showed a significantly higher proliferating activity than those treated with OVA alone. These results suggest that EN-3 may possess adjuvant activities to potentially to enhance humoral as well as cellular immune response.

ENAMEL MICROABRASION

김신,염정현,하은숙,김동규,최외임 大韓小兒齒科學會 1992 大韓小兒齒科學會誌 Vol.19 No.2

심하게 파손된 제1대구치의 발거에 관하여

염정현,최외임,김동규,하은숙,김신 大韓小兒齒科學會 1992 大韓小兒齒科學會誌 Vol.19 No.2