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심병식,최정아,Ho-Hyun Song,박성무,천인수,Ji-Eun Jang,Sun Je Woo,Chung Hwan Cho,송민석,김혜미,Kyung Joo Song,이재면,김성욱,송대섭,최영기,김재욱,Huan Nguyen,김동욱,Young Yil Bahk,윤철희,송만기 한국미생물학회 2013 The journal of microbiology Vol.51 No.1
Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a wellestablished bacterial expression system and administered it into mice via sublingual (s.l.) route. We found that s.l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i.m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s.l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.
조영제,안봉전,최정 ( Young Je Cho,Bong Jeun An,Cheong Choi ) 생화학분자생물학회 1993 BMB Reports Vol.26 No.3
For the purpose of utilizing tannins in the functional foods and crude drudgs, chemical structure of tannins isolated from Korean green tea were determined. Acetone extract from Korean green tea showed inhibition effect against the angiotensin converting enzyme and xanthine oxidase. The acetone extract, on the separation by the use of Sephadex LH-20, MCI-gel CHP-20, resulted in six kinds of purified tannin and all of them are condensed tannins. Purified tannins were identified to be (+)-catechin, (-)-epicatechin-3-O-gallate, (-)-epigallocatechin-3-O-gallate, (+)-gallocatechin, (-)-epigallocatechin, procyanidin B-3-3-0-gal-late by PMR spectrum, IR spectrum and Mass spectrum. The galloyl tannins showed higher inhibition activity against angiotensin converting enzyme and xanthine oxidase than the no-ngalloyl tannins.
Bacillus sp . CW - 1121 이 생성하는 Alkaline Protease 의 특성 및 작용양상
이우제,조영제,손규목,최정 ( Woo Je Lee,Young Je Cho,Gyu Mok Son,Cheong Choi ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.6
An alkaline protease producting bacteria was isolated from soil and identified as Bacillus sp. CW-1121. It was found that the production of alkaline protease reached to maximum when casein, (NH₄)₂HPO₄, MgSO₄, fructose as organic and inorganic nitrogen, inorganic salt and carbon source added in culture medium. The optimum temperature and pH for the purified enzyme activity were 40℃ and pH 10.0 respectively. The enzyme was relatively stable in the pH range of pH 9.0∼11.0 and at the temperature below 50℃. The activity of the purified enzyme was inhibited by Hg^(2+) and Fe^(2+), whereas Mn^(2+) and K^+ gave rather activating effects on the enzyme activity. PCMB, 2,4-DNP, ε-aminocaproic acid did not show inhibitory effect in the proteolytic activity of alkaline protease but ethylenediaminetetraacetic acid inhibited the enzyme activity. The reaction of this enzyme followed typical Michaels Menten kinetics with the K_m value of 1.25×10^(-4) M/1 with the V_(max) of 7.14 ㎍/min for casein.