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Anabaena cylindrica 분해세균의 분리 및 동정
최영길,홍엽,신규철,김민성,한명수,Choi, Yong-Keel,Hong, Yup,Shin, Kyu-Chul,Kim, Min-Seong,Han, Myung-Soo 한국미생물학회 2002 미생물학회지 Vol.38 No.2
To isolate the bacteria lysing cyanobacteria, the sediment samples were collected from Dochang and Pal'tang Reservoir and Seokchon Lake. Each sample was smeared on the Anabaena cylindrica lawn and incubated in light chamber for 11 days. Bacteria having cyanobacteria-Iysing activity were isolated from the samples of Seokchon reservoir. Confirmation of cyanobacteria-Iysing activity was carried out to measure chlorophyll a and bacterial cell counting in mixed culture of Anabaena cylindrica and bacteria. Lysis was detected when extracellular meterials was added to the Anabaena cylindrica culture. The isolate was identified by analysis based on 16S rDNA sequence and morphological and physiological properties. The bacterial strain was taxonomically studied by the phylogenetic analysis based on 165 rDNA sequence. This strain was identified as a member of the genus Bacillus and designated as Bacillus sp. CHS1. 남조류 분해세균을 분리하기 위하여 도창, 팔당 저수지와 석촌 호수로부터 저층시료를 채취하였다. Anabaena cylindrica lawn에 각각의 시료를 도말한 후 11 일간 배양하였고 석촌 시료를 접중한 Anabaena cylindrica lawn에서 Anabaena cylindrica에 분해능을 가지는 세균을 분리하였다. 분해능 화인은 남조류와 분해세균을 혼합한 후 chlorophyll a간의 측정과 분리세균의 cell counting방법으로 확인하였고, 세포외 물질이 Anabaena cylindrica를 분해하는 것을 확인하였다. 분리균주는 16S rDNA 염기서열 분석과 형태학적, 생리학적 특징을 기초로 하여 동정하였다. 분리균주의 165 rDNA염기서열을 기초로한 분자계통학적 분석을 통하여 Bacillus속에 포함하는 계통학적 그룹에 속한다는 것을 확인하였고, Bacillus sp. CHSl으로 명하였다.
한국인의 비만과 인슐린비의존성당뇨병에서 베타-2 아드레날린 수용체 유전자 다형성
김병준(Byoung Joon Kim),안규정(Kyu Jeong Ahn),김영설,최영길,이문규,김성훈(Sung Hoon Kim),김동준(Dong Jun Kim),함종렬(Jong Ryeal Hahm),정재훈(Jae Hoon Chung),민용기(Yong Ki Min),이명식(Myung Shik Lee),김광원(Kwang Won Kim) 대한내과학회 1999 대한내과학회지 Vol.56 No.4
N/A Objective: Catecholamine play a central role in the regulation of energy expenditure, in part by stimulating lipid mobilization through lipolysis in fat cells. The beta-2 adrenergic receptor(BAR-2) is a major lipolytic receptor in human fat cells. A recent study has shown that common polymorphisms occuring at codon 16 and 27 of the BAR-2 gene are significantly associated with obesity and lypolytic BAR-2 function in adipose tissue. We investigated whether the previously described human BAR-2 gene polymorphisms are associated with obesiy and NIDDM in Koreans. Methods : All subjests were divided into two groups, non-obese and obese group, according to their body mass index. And their clinical characteristics were evaluated. The BAR-2 gene polymorphisms were analyzed by PCR-RFLP in 89 nondiabetics and 106 patients with NIDDM. Results : When the allele frequency of BAR-2 gene polymorphisms was compared with that of western people, there was a significant difference. In our study, there was no significant difference in the allele frequency of BAR-2 gene polymorphisms at codons 16 and 27 between nonobese and obese group both nondiabetics and NIDDM subjects. The frequency of Glu27 homozygotes was very rare(1.1%). Body mass index(BMI), waist-hip ratio(WHR), and serum glucose and insulin secretion of the nondiabetics with polymorphism of codon 16 or codon 27 did not differ from those of the subjects without the polymorphisms. In NIDDM group, the Gly16 homozygotes had a lower BMI than Arg16 homozygotes without any difference in WHR and the other laboratory parameters. Neither clinical or laboratory parameters of the diabetics with the polymorphism at codon 27 differ from those of subjectes without the polymorphism. Conclusion : These findings suggest that the genetic variability in the human BAR-2 gene is not a major determinant for the development of obesity and NIDDM in Koreans.