Home-made 요구르트와 시판 중인 요구르트에서 분리한 젖산균의 기능적 특성 조사

최문섭,윤현명,오계헌,Choi, Moon-Sup,Yun, Hyun-Myoung,Oh, Kye-Heon 한국미생물학회 2014 미생물학회지 Vol.50 No.1

본 연구는 home-made 요구르트에서 분리한 Lactobacillus casei SK-7과 시중에서 판매되고 있는 요구르트에서 분리한 Lactobacillus bulgaricus YK-11의 기능성을 비교 분석하기 위해 실시하였다. 먼저 분리세균인 SK-7과 YK-11의 생리화학적 특성조사를 진행하였다. 이들 균주는 16S rRNA 염기서열을 이용한 계통유전학적 분석방법으로 동정하여, 각각 L. casei SK-7와 L. bulgaricus YK-11로 명명하였다. 16S rRNA 염기서열을 바탕으로 SK-7과 YK-11의 유전적 계통수를 작성하였다. 72시간 동안 SK-7과 YK-11 배양에서 lactic acid와 acetic acid의 생산, 그리고 pH 변화를 조사하였다. 배양기간 동안 L. casei SK-7과 L. bulgaricus YK-11의 몇가지 기능성을 조사하였다. L. casei SK-7과 L. bulgaricus YK-11 배양상등액은 주어진 nitrite를 각각 93.9%와 88.2% 소거하였다. SK-7 and YK-11 배양상등액의 항산화능은 각각 62.6%와 54.9%였으며, ${\beta}$-galactosidase 활성은 14.9 units/mg과 13.1 units/mg로 측정되었다. 항미생물능력은 SK-7과 YK-11 배양의 20배 배양농축액으로 조사되었으며, SK-7은 이 조사에 사용된 모든 미생물에서 명백한 것으로 관찰되었으나, YK-11에서는 관찰되지 않았다. 부가적인 기능성 연구가 이루어져야 되겠지만, 본 연구에서는 home-made 요구르트에서 분리된 젖산균의 기능성은 시판요구르트에서의 기능성과 비교하여 우수한 것으로 조사되었다. The objective of this work is to investigate and compare several functional properties of lactic acid bacteria (LAB), Lactobacillus casei SK-7 isolated from home-made yogurt and Lactobacillus bulgaricus YK-11 from commercial yogurt. Initially, physiological and biochemical properties of SK-7 and YK-11 were characterized. Phylogenetic analysis using 16S rRNA sequencing were performed to identify the strains, and the strain could be assigned to Lactobacillus casei and Lactobacillus bulgaricus, designated as L. casei SK-7 and L. bulgaricus YK-11. Phylogenetic tree of SK-7 and YK-11 was plotted based on 16S rRNA sequence comparisons. Production of lactic acid and organic acid, and pH changes in the cultures of SK-7 and YK-11 were monitored during 72 h. During the incubation period, several functional properties of L. casei SK-7 and L. bulgaricus YK-11 were examined. L. casei SK-7 and L. bulgaricus YK-11 cultures eliminated 93.9% and 88.2% of nitrite, respectively. Antioxidant activity of cultural supernatants of SK-7 and YK-11 were 62.6%, 54.9%, and activity of ${\beta}$-galactosidase were 14.9 units/mg and 13.1 units/mg, respectively. The antimicrobial activities were examined with 20-fold concentrated culture supernatants from the cultures of SK-7 and YK-11. The activities of SK-7 supernatants were clearly observed against all microorganisms in this work, whereas no activities were observed in YK-11 supernatants. Although it might be conducted additional functional research, functional properties of LAB isolated from home-made yogurt have been shown to be better than those of commercial yogurt in this work.

청주 역사적 도심지구의 수로체계 변천과정

최문섭(Choi Mun-Seop),김태영(Kim Tai-Young) 대한건축학회 2011 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.31 No.2(계획계)

The waterway system in traditional urban area on Cheong-ju city district is divided into two periods such as 1911-1966 and 1967-2011 present. It have been inclined to improve urban landscape and infrastructure-establishment in the urbanization process since 1967, whereas it was focused on solutions of basic problems such as preparation for danger against natural disasters and livelihood arrangement before 1967. The destroyed waterway system will be one of factors that suggest an image of the future in traditional urban area on Cheong-ju city that is a sustainable city as a factor that should be maintained in the process of urban change in the future.

문화재 지정 초등학교 교사의 지속적 사용에 관한 조사연구

최문섭(Choi Mun-Seop),김태영(Kim Tai-Young) 대한건축학회 2009 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.29 No.1(계획계)

This study is to grasp the actual condition of three elementary school buildings to be being used up to now, of ten cases of elementary school building designated as the modern architectural properties. We drawn up checklist associated with elementary school building on the basis of the Architectural planning theory and relevant laws, and investigated the present condition that the elementary school building designated as modern architectural properties is being used in the present living and service constantly by the process of transition, and knew why it is possible to use constantly, also later on to use constantly we have inquired what parts should be considered. In result, the parts to be changed, as making cheerful surroundings by achieving the efficiency of educational activity and the improvement of educational environment, were seen that the aspects of facility are the main part. Through the illumination that is influential with environment of studying, the air-conditioning- process-change for improving the life level and electrical communication equipment, it is possible to use constantly the elementary school building designated as moden architectural properties, and first of all if we make the process of maintenance simply and make system to be able to respond with the problem, the constant use of elementary school building designated as moden architectural properties is judged to be possible.

발효된 물김치인 동치미에서 분리한 혼합 젖산균의 생리활성 증진에 대한 연구

최문섭(Moon-Seop Choi),김동민(Dong-Min Kim),오계헌(Kye-Heon Oh) 한국생물공학회 2015 KSBB Journal Vol.30 No.5

The aim of this study was to investigate the efficacy of enhanced physiological activities in cultures isolated from Korean fermented watery Kimchi, Dongchimi, of single lactic acid bacteria (LAB), and when these three are mixed LAB as probiotics. Using the BIOLOG system and 16S rRNA sequencing, the isolates were characterized, and identified and assigned to Leuconostoc mesenteroides DK-3, Leuconostoc dextranicum DK-6, and Lactobacillus curvatus DK-13, respectively. Growth rate and pH changes, production of organic acids as metabolites, and physiological activities of the single and mixed LAB cultures, were monitored and compared. In mixed LAB cultures after 72 h of incubation, the maximum concentrations of lactic acid and acetic acid were approximately 340.5 mM and 191.9 mM, respectively, and pH changed from 7.00 to 3.62. Mixed LAB cultures were able to eliminate 96.3% of nitrite. Activities of antioxidant and β-galactosidase were 60.3% and 16.8 units/mg, respectively. Significant antibacterial activity of the concentrated supernatants was demonstrated against several food-poisoning bacteria. Physiological activities obtained from the mixed LAB cultures have been shown to be considerably higher than those of single LAB cultures. In conclusion, these studies demonstrate that compared to the single cultures, all physiological activities in mixed LAB cultures are significantly enhanced.

인수기업의 지배구조와 피인수기업의 주주가치

최문섭 ( Moon Sub Choi ) 한국재무관리학회 2011 財務管理硏究 Vol.28 No.2

기업지배구조이론에 의하면 열악한 지배구조를 갖는 기업의 경영진일수록 비합리적인 기업투자결정을 내린다는 것이 알려져 있으며, 경영권 방어조항(antitakeover provision, ATP)이 기업정관에 많은 기업이 이에 해당된다는 연구결과가 문헌에 존재한다. 본 연구는 ATP조항이 많은 미국 기업들일 수록 미국과 해외의 상장기업에 대한 인수 발표 시 피인수기업의 누적초과수익률(cumulative abnormal return)이 높다는 결과를 얻었으며, 해외 피인수기업의 수익률이 미국기업의 그것을 평균적으로 상회함을 보여준다. 마지막으로, 이러한 결과는 인수기업 내부정보 유출의 가능성에 대해서도 강건함(robustness)을 보여준다. The theory of corporate governance suggests that managers of poorly governed firms are more likely to make poor investment decisions, and the evidence on high antitakeover provision (ATP) firms is consistent. I study the effect of domestic and foreign takeovers by U.S. firms and find that high-ATP bidders tend to pay relatively high premiums for their targets. Also, foreign targets` event-study returns are significantly higher than those of U.S. domestic ones. Finally, these findings are robust to controlling for acquirers` exposure to insider trading.

산업폐수로부터 분리한 1,4-Dioxane을 분해할 수 있는 새로운 세균인 Shinella granuli CK-4의 특성

최문섭(Moon-Seop Choi),최기승(Ki-Seung Choi),오계헌(Kye-Heon Oh) 한국생물공학회 2021 KSBB Journal Vol.36 No.1

We have characterized a novel bacterium, strain CK-4 isolated from industrial wastewater, which is capable of utilizing 1,4-dioxane as sole carbon source and energy. Complete depletion of 1 g/L 1,4-dioxane by CK-4 cultures growing on AMS media was achieved within 168 h. Both the Biolog system and phylogenetic analysis using 16S rRNA sequencing were utilized for identification, and the strain was designated as Shinella granuli CK-4, and registered in GenBank as [MN841914]. The mortality of CK-4 was not greatly affected by 1,4-dioxane concentrations below 10 g/L but was increased with increasing 1,4-dioxane concentrations. SDS-PAGE revealed that the amount of lipopolysaccharide in CK-4 increased or decreased according to the 1,4-dioxane concentration. The expression of 60-kDa GroEL stress shock protein (SSP) was increased in proportion to concentrations below 10 g/L 1,4-dioxane but was not expressed at concentrations above 15 g/L dioxane. On the other hand, 70-kDa DnaK SSP was not expressed at any 1,4-dioxane concentrations (0~20 g/mL) tested. SEM demonstrated that the surface of CK-4 cells exposed to 10 g/L dioxane was not significantly different from that of normal cells, whereas cells exposed to 20 g/L 1,4-dioxane showed the presence of perforations and irregular rod forms with wrinkled cell surfaces. These results provide clues to understanding the resistance of S. granuli CK-4 to 1,4-dioxane and the promotion of 1,4-dioxane degradation, which can be applied to the treatment of industrial wastewater with higher concentrations of 1,4-dioxane.

벤치규모 생물반응기에서 Shinella granuli CK-4에 의한 1,4-Dioxane의 생분해에 영향을 미치는 물리화학적 요인

최문섭(Moon-Seop Choi),최기승(Ki-Seung Choi),오계헌(Kye-Heon Oh) 한국생물공학회 2021 KSBB Journal Vol.36 No.2

Our previous research has demonstrated that Shinella granuli CK-4 is capable of utilizing 1,4-dioxane as its sole source of carbon and energy when isolated from industrial wastewater [1]. In this study, we have extended this work to investigate the relationships between 1,4-dioxane degradation and the strain CK-4 and several relevant physicochemical environmental parameters in 3 L bench-scale bioreactors. One gram of 1,4-dioxane per liter was completely degraded within 96 hours of incubation, and the optimum temperature for 1,4-dioxane degradation was 30oC. 1,4-Dioxane degradation by CK-4 gradually increased as a function of airflow rate and agitation speed, and the optimum airflow rate and agitation speed were 2.5 L/min and 500 rotation per minutes, respectively, in this study. The addition of 50 mg/L tetrahydrofuran as a supplemental carbon source was found to stimulate the maximal bacterial growth and 1,4-dioxane degradation. The optimum results obtained from these experiments were combined and applied for 1,4-dioxane degradation. As a result, the complete degradation of 1,4-dioxane was achieved with the CK-4 culture within 72 hours of incubation. We explored the feasibility of using the cultures of S. granuli CK-4 for the degradation of 1,4-dioxane with the aim of microbial application in industrial wastewater treatment in bench-scale bioreactors.

알지네이트 스펀지 세포지지체에서 인체 지방조직 추출 줄기세포의 연골분화

최문섭 ( Moon Seop Choi ),전영준 ( Young Joon Jun ),조현미 ( Hyun Mi Cho ),오득영 ( Deuk Young Oh ),안상태 ( Sang Tae Ahn ),한동근 ( Dong Keun Han ),이종원 ( Jong Won Rhie ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4

Recently it has been proven that population of stem cells obtained from stromal vascular fraction of fat tissue have the multilineage potential which is a capability of differentiation into bone, cartilage, fat, and muscle. In addition, a surgeon can easily harvest human adipose tissue using by minimal invasive technique such as a liposuction. In this article, I hypothesized that human adipose tissue contains the stem cells and has an capability of chondrogenic differentiation in alginate sponge scaffolds. Alginate sponge scaffolds were made by lyophilizing of alginate throughout alginate gel state and human adipose tissue was obtained from a liposuction. In experimental group, human adipose-derived stem cells in alginate sponge scaffolds, were differentiated with chondrogenic medium consisted of transforming growth factor-β1 and dexamethasone. Control group was defined as a culture with complete medium. After differentiation at 1, 2, and 3 weeks, gene expression was examined by reverse transcriptase polymerase chain reaction(RT-PCR) for collagen type II and link. In addition, content of glycosaminoglycan and Alcian blue stain were carried out for confirming of chondrogenesis. RT-PCR results show gene encodings of collagen type II and link in experimental group. The content of glycosaminoglycan in experimental group was greater than that in control group. In Alcian blue stain, sulfated proteoglycan was stained with blue color in experimental group. Consequently it was proven that adipose-derived stem cells have a capability of chondrogenic differentiation in alginate sponge scaffolds. In a view point of cell therapy, tissue engineering of adipose-derived stem cells is able to apply for reconstruction of tissue defects, congenital, and esthetics.

기업투명성과 신약 개발효과

최문섭(Choi, Moon Sub),이종국(Lee Jongkuk),김준희(Kim, Junhee) 한국상품학회 2012 商品學硏究 Vol.30 No.4

신제품 개발은 기업의 지속적 경쟁우위 및 기업 생존을 위한 핵심요소로 강조되어 왔다. 또한, 기존 연구들은 기업 경영의 투명성이 기업 자원의 효율적 활용을 위한 바탕이 됨을 제시하고 있으나, 기업투명성이 신약개발의 효과에 미치는 영향에 대한 실증적 연구는 없는 실정이다. 이에, 본 연구는 제약업체의 기업투명성이 신약허가 발표시 해당 종목의 조사기간 수익률에 어떠한 영향을 미치는가에 대해서 규명한다. 투자자들이 기업 내부정도에 노출된 정도를 통제했을 때, 신약의 혁신유형은 각기 상이한 경제적 부가가치를 창출하는 것으로 보인다. 또한, 주가수익률 동기성으로 추정된 기업투명성이 높은 제약업체일 수록 산약 허가발표시의 누적초과수익률 또한 개선된다. New product innovations are a key source of sustainable competitive advantage and firm survival. Although corporate transparency has been emphasized for efficient use of firm resources, we know little about the link between corporate transparency and firm value effect of new product innovations. In response, we assess whether corporate transparency of NYSE-listed pharmaceutical companies affects event-study returns upon announcements of FDA approvals. Controlled for investors’ relative exposure to inside information potentially pertaining to approval procedure, innovation types are priced in the announcement returns. Lastly, announcement returns are lower with a pharmaceutical firm lower in corporate transparency, proxied for by stock return synchronicity.

다중요인모형을 활용한 대만증시 상장종목에 관한 실증분석

최문섭 ( Moon Sub Choi ),( Seth H. Huang ),전예리 ( Yeri Jeon ) 아시아.유럽미래학회 2016 유라시아연구 Vol.13 No.4

문헌에는 위험요인으로써 자산수익률 예측을 모형화하는 많은 연구가 있다. 현대 포트폴리오 이론이 도입된 후 자산가격평가모형은 단변량에서 다변량 구조로 변모해왔다. 이론적 모형의 발전과 더불어 이의 실증적 검정 및 여러 국가별 자본시장에의 응용 또한 다양하게 이루어져 왔다. 하지만 아시아의 신흥시장에 관간 연구의 경우 지역에 특화된 모형화와 실증 모형의 식별이 더욱 절실하다. 현재까지는 업종별 분류 포트폴리오 수익률 위주로 분석이 이루어졌으며 최근 들어 다요인 모형의 활용이 늘어나긴 했지만 여전히 문헌상 기록된 연구의 수효가 미미하다. 이에 본 연구에서는 대만증시에 초점을 맞추어 여러 모형의 유용성을 점검해 본다. 대만증시에 관한 기존의 연구에서는 시장할증요인(market premium factor)이 주식 수익률에 큰 영향을 미치는 반면 가치주할증요인(“high-minus-low.,” HML) 또는 소형주할증요인(“small-minus-big.,” SMB)의 효과는 미미한 것으로 나타나 있다.(Ko, 2014; Cheng, 2006) 관성요인(“up-minus-down.,” UMD)에 관해서는 좀 더 면밀한 관찰이 필요하다. 아울러 Fama and French(1997)의 미국 증시에 관한 연구에서 업종별 분류 포트폴리오 수익률로써 자산가격평가모형을 수립하는 것은 여타 분류 포트폴리오수익률에 기반한 것과 비교한 실증분석 결과를 보고하였다. 유럽 증시에 대해서도 비슷한 분석 결과가 있다.(Moerman, 2005) 아시아 신흥시장에서의 업종별 분류 포트폴리오 수익률에 대한 분석은 여전히 수적으로 제한적이며 추가적인 연구가 요구된다. 본 연구에서는 대만증시 상장주식으로 구성된 포트폴리오에 대한 다중모형을 활용한 실증분석을 수행한다. 표본은 대만증권거래소(Taiwan Stock Exchange, 이하 TWSE)에서 2001년 7월부터 2015년 12월사이에 거래된 모든 상장종목이다. Moerman(2005)의 분류방식에 의해 대만증시의 업종별 포트폴리오를 구성한 후에 각 포트폴리오별 설명요인을 식별하기 위해 시장모형, Fama and French(1993)의 3요인모형 및 Carhart(1997)의 4요인모형으로써 회귀분석을 수행하였다. 다양한 표본기간에 상이한 요인모형으로써 대만증시 상장종목을 실증분석한 결과에 의하면 업종별 양상이 두드러짐을 알 수 있었다. 그럼에도 불구하고 Fama and French(1997)의 결과와 대동소이하게도 여러 표본기간에 대해서 각 요인모형의 상대적 우월성은 나타나지 않았다. 전반적으로 시장모형의 요인이 업종별 수익률을 설명하는데 있어 통계유의적인 영향을 미친다는 결론을 얻었으며 다른 요인들의 영향력은 제한된 것으로 나타났다. 이는Cheng(2006)의 결과에 부합한다. Large number of studies have been performed to model asset returns in terms of risk factors. After the emergence of modern portfolio theory, asset pricing models have been evolved from single factor to multi-factors. Along with development of the models, validation of models with various portfolios and regions continued in wide range. However, researches in emerging Asia-Basin markets still need considerable amount of regional modeling and verification. Also, limited number of studies were conducted on industry sorted portfolio returns and more recent multi-factor models in particular. Therefore, this study focuses on validation of several market models in Taiwan stock market. This paper applies empirical tests with several well-known multi-factor models on portfolios sorted by industries in Taiwan stock market. Sample data includes all listed stocks collected from Taiwanese Stock Exchange (TWSE) from July 2001 to December 2015. Following Moerman (2005), industry portfolio is constructed based upon TWSE`s industry group classification codes per FTSE codes. To compare effect of each model in different portfolios, capital asset pricing model (CAPM), Fama and French`s (1993) 3 factor model, and Carhart`s (1997) 4 factor model are used in the study. In previous studies of the Taiwan stock market, it is known that market premium (MP) factor shows significant influence on return and relatively weak support to the effects of “high-minus-low” value premium (HML) and “small-minus-big” size premium (SMB) factors (Ko, 2014; Cheng, 2006). However, effect of the momentum factor (“up-minus-down.,” UMD) needs more validation. Also, as Fama and French (1997) discussed in U.S. market, returns of portfolios constructed in respect to industry sector is not very accurate when compared to other portfolio returns. Comparable results were observed in the European markets (Moerman, 2005). Industry sorted returns in emerging Asia-Basin markets were mostly done in limited extent and need further research. Empirical results from various time period samples have shown some differences between industries exist when different factor model is tested in Taiwan market. When whole period sample is used, influence of MP factor prevails over other factors. SMB, HML and UMD factor show significance in certain industries. In 5-year basis period sample, 2001-2005 and 2006-2010 periods show results similar to whole period, but period from 2011 to 2015 deviates from formal results with significant UMD factor in number of industries. However, similar to result of Fama and French (1997), there is no evidence of clear winner on different models in all time periods in terms of model fitness. Overall, consistent with findings of Cheng (2006), MP factor has significant influence in explaining industry return. Effects of other factors are limited with weak influence.