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Influence of Coating Ligands onf Enzyme-linked Immunosorbent Assay of Toluene
김소영,이남택,최명자,Kim, So Yeong,Lee, Nam Taek,Choe, Myeong Ja Korean Chemical Society 2001 Bulletin of the Korean Chemical Society Vol.22 No.9
The specificity of conjugation site for coating ligands was investigated using toluene-bovine serum albumin (BSA) conjugates in which BSA was conjugated at the position of o-, m-, and ${\rho}-toluic$ acid. Toluene-BSA conjugated at ${\rho}-position$ showed a binding activity of about 89-95%, whereas, those conjugated at o- and m-position of toluene exhibited a binding activity of 5 and 11%, respectively. On the basis of the above result, coating ligands with various proteins (OVA, BSA, KLH) were compared by conjugating with $\rho-toluic$ acid, and toluene-KLH was considered as the best coating ligand for this ELISA. Indirect competitive ELISA method was developed using anti-toluene antibody and $\rho-position$ conjugated toluene-KLH. The dose-response curve constructed after kinetic and optimization studies showed a 1${\times}$10-4 - 1${\times}$102 mM detectable response range with 0.1 ${\mu}M$ detectability. In specificity test of the antibody, the binding capabilities of aromatic compounds substituted with nitro-, alkyl-, chloro-, and hydroxyl group were larger rather than those of aromatic compounds (benzene, toluene and xylene) themselves. Also, tests with soil and water samples that had been spiked with toluene resulted in 102.7-113.7% recovery.
혈소판 당단백 2b/3a 수용체 차단제(Abciximab : ReoPro(R)) 부착 관상동맥 스텐트의 장기 임상 결과
김원 ( Kim Won ),정명호 ( Jeong Myeong Ho ),홍영준 ( Hong Yeong Jun ),이승현 ( Lee Seung Hyeon ),박우석 ( Park U Seog ),김주한 ( Kim Ju Han ),김인수 ( Kim In Su ),최명자 ( Choe Myeong Ja ),안영근 ( An Yeong Geun ),조정관 ( Jo Jeo 대한내과학회 2003 대한내과학회지 Vol.65 No.6
목적: Abciximab (ReoPro(R))은 강력한 혈소판 응집 억제제일 뿐만 아니라 혈관 내피세포나 대식 세포의 CD11b/18 수용체에 작용하여 염증반응을 억제하며, 혈관 평활근 세포의 증식을 억제하는 효과가 있다. 관상동맥 중재술을 시행 받는 관상동맥질환자에 있어서 ReoPro(R)부착 스텐트의 장기 임상결과와 안정성을 평가하고자 하였다. 방법: 무작위 전향적인 연구로서 2001년 8월부터 2003년 6월까지 전남대학교병원 심장센터에서 관상동맥 Background: Previously we reported the inhibition of coronary restenosis with Abciximab(ReoPro(R))-coated stent in a porcine model. ReoPro(R) inhibits platelet aggregation, the proliferation of vascular smooth muscle cells and inflammatory reaction. Metho