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최근준,왕창근 忠南大學校 環境問題硏究所 1999 環境硏究 Vol.17 No.-
Phosphorus in natural water and wastewater exists in supersaturated state due to low solubility, and it is difficult to form crystals and precipitates because precipitation rate in steps of nucleation, crystal growth, agglomeration and ripening is slow. Precipitation rate can be increased by seeding the crystals which has components and structure similar to the precipitates. This study is based on the precipitation mechanism and was performed to see phosphorus removal efficiency of various clamshells serving as surface precipitation media. The results obtained from this study are following ; From XRD analysis for various clamshells, major component of clamshell used as phosphorus removal material is found to be CaCO₃/aragonite. The batch tests with the clamshells showed excellent phosphorus removal efficiency up on 99% depending on the experimental condition. Phosphorus removal efficiency was dependent on temperature, detention time, pH and shell particle size, and appeared relatively high removal efficiency at high temperature, long detention time, low pH and small shell particle size. Phosphorus removal technology using clamshells has many advantages of reusing solid wastes, not producing sludge as well as not adding chemicals.
무선통신모듈과 스마트폰 application을 이용한 RGB-LED와 sensor 컨트롤
황준연,최근준,원진영,황인철 강원대학교 정보통신연구소 2015 정보통신논문지 Vol.19 No.-
In modern society, a variety of techniques using a plurality of LED control technology, sensor and a technique utilizing a smart-phone application in the wi-fi environment has evolved. If you use the above technique, it will be able to control lighting decoration more convenient by transmitting and receiving color information of the information and LED obtained from sensor wirelessly consists of LED. In this design, wide wi-fi wireless communication module and sensor by combining control system utilizing information remote LED color and brightness in the obtained from sensor environment to has been used LED control functions in real life to try to implement a system that can be controlled.
Jin-Ik Kim(김진익),Yong-Won Choi(최용원),Geun-June Choi(최근준),Ji-An Kang(강지안),In-Young Lee(이인영),Nandintsetseg Narantuya(나란투야 난딘셋세그),Myong-Seok Oh(오명석),Sik-Jae Cho(조식제),Ja-Young Moon(문자영) 한국생명과학회 2021 생명과학회지 Vol.31 No.1
본 연구는 Peucedanum insolens Kitagawa 뿌리의 여러 유기용매 분획물에 대한 항산화 활성과 LPS 처리된 RAW264.7 세포에서 항염증 효과를 조사하기 위해 수행되었다. 이 연구를 위해 P. insolens Kitagawa 뿌리를 먼저 실온에서 1 주일 동안 건조하여 잘게 썰고 70% 에탄올로 추출한 다음 hexane, chloroform, ethyl acetate 및 물로 연속적으로 분획하였다. DPPH (1,1-diphenyl-2-picrylhydrazyl) 라디칼 소거능 분석과 total polyphenol 및 total flavonoid 함량을 측정하여 유기용매 분획물의 항산화 활성을 평가했다. RAW264.7 세포에서 iNOS, COX-2, IL-1β 및 IL-6과 같은 염증 매개 유전자의 발현 억제 수준을 측정하여 분획의 항 염증 효능을 평가했다. 본 연구 결과는 P. insolens Kitagawa 뿌리의 ethyl acetate 분획물이 상대적으로 높은 함량의 total flavonoid (34.08±1.68 μg QE/mg)와 total polyphenol (154.1±3.2 μg의 GE/mg)을 함유하고 있음을 분명히 보여주었다. DPPH 분석 결과 P. insolens Kitagawa는 ethyl acetate 분획에서 강력한 자유 라디칼 소거 활성을 가지고 있음을 보여주었다. Ethyl acetate 분획 및 hexane 분획은 모두 RAW264.7 세포에서 24시간 동안 LPS (1 μg/ml) 처리에 의해 유도된 산화질소 생성에 대해 강력한 억제력을 나타냈다. 본 연구결과는 또한 P. insolens Kitagawa의 hexane 분획 및 ethyl acetate 분획 모두 RAW264.7 세포에서 24시간 동안 LPS 처리에 의해 과발현된 iNOS, IL-1β 및 IL-6의 mRNA 수준을 강력하게 억제하는 것으로 나타났다. 이러한 결과는 P. insolens Kitagawa가 항 염증 활성에 강한 효능을 가진 화합물을 포함할 수 있음을 시사한다. 염증 조절에 대한 P. insolens Kitagawa 분획물의 더 자세한 효과를 이해하기 위하여 이 분획물이 cytokine 신호 전달 경로 및 염증 신호 경로에 대한 조절작용과 같은 추가적인 작용기전 연구가 필요하다. This study was performed to investigate the antioxidant activities of subfractions of Peucedanum insolens Kitagawa root in various organic solvents and their anti-inflammatory effects on LPS-treated RAW264.7 cells. First, P. insolens Kitagawa roots were dried at room temperature for one week, chopped, and extracted with 70% ethanol. The resulting extracts were successively sub-fractionated with hexane, chloroform, ethyl acetate, and water. The antioxidant potential of the fractions was evaluated using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay and by measuring total polyphenol and flavonoid contents. The anti-inflammatory potency of the fractions was evaluated by measuring the inhibition levels of the expressions of inflammatory-mediated genes and proteins (e.g., iNOS, COX-2, IL-1β, and IL-6) in RAW264.7 cells. The results clearly showed that the ethyl acetate fraction of the P. insolens Kitagawa root contained relatively high total flavonoid (34.08±1.68 μg of quercetin equivalents per mg) and total polyphenol (154.1±3.2 μg of gallic acid equivalents per mg) contents. The DPPH assay results showed that the P. insolens Kitagawa root possessed strong free radical scavenging activity in the ethyl acetate fraction. Both the ethyl acetate and hexane fractions showed strong inhibitory potencies to nitric oxide production induced by lipopolysaccharide (1 μg/ml) treatment for 24 hr in RAW264.7 cells. The results also showed that both the hexane and ethyl acetate fractions of the P. insolens Kitagawa root strongly inhibited mRNA levels of iNOS, IL-1β, and IL-6, which were overexpressed by LPS treatment for 24 hr in the RAW264.7 cells. These results suggest that P. insolens Kitagawa root may contain compounds that possess strong potency for anti-inflammatory activity. Further studies are needed to discover more detailed modes of action of P. insolens Kitagawa root fractions against inflammation modulation, such as the regulation of cytokine signaling and inflammatory signaling pathways.