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부산지방 성인의 Helicobacter pylori 보균 실태
최경현(Kyung Hyun Choi),이상호(Sang Ho Lee),박영훈(Young Hun Park) 대한소화기학회 1996 대한소화기학회지 Vol.28 No.6
N/A Background/Aims: In recent years much work has been done on the link between Helicobacter pylori and gastric diseases. In order to study the prevalence of Heliccbacter pylori infection in normal Koreans,we determined the anti-Helicobacter pylori imnunoglobulin G levels in 664 ernployees working in Kosin medical center, Pusan Korea. Methods: Helicobacter pylori Ig G antibodies w=re asssayed by enzyme linked immunosorbent assay(ELISA), and ABO blood group was examined. Results: Overall seropositivity was 79.1%. The prevalance of infection increased with aging (p<0.05). There was no relationship between ABO blood group and Helicobacter pylori seropositivity. Conclusions: There was high prevalence of Helicobacter pylori infection among Korean adults. (Korean J Gastroenterol 1996;28:758 - 763)
위 질환 환자들에서 분리된 Helicobacter pylori 의 공포화 독소 ( Vacuolating Toxin ) 생성
김충기(Chung Kee Kim),정승수(Sung Soo Jeong),이겸철(Gyum Cheol Lee),서대홍(Dae Hong Suh),송갑영(Gap Young Song),최경현(Kyung Hun Choi),장명웅(Myung Woong Chang) 대한내과학회 1998 대한내과학회지 Vol.54 No.5
N/A Objectives: This study was carried out to survey the prevalence of Helicobacter pylori infection and the incidence of vacuolating toxin producing H pylori. A further aim of this study was to evaluate the quantitative assay for cell vacuolation on the basis of the rapid uptake of neutral red dye by vaculoes of the cells. Methods: We studied the gastric biopsy specimens of patients with 154 cases of gastritis, 74 cases of gastric ulcer, and 167 cases of gastric cancer and in 44 cases of healthy persons, One of the biopsy specimen was placed into a CLOtest plate for rapid urease test and the other one of the biopsy spcimen was inoculated on Brain Heart Infusion blood agar for culture. The culture supernatant of isolated H. pylari was serially diluted with BHI broth. After 24 hour incubation of cultured RK-13 cells treated with the culture supernatgnt of H. pylori, cytoplasmic vacuolation of the cells were observed microscopically. Results: The positivity of urease test and the rate of isolation of K pylori from urease positive gastric biopsy materials were 34.1% and 93.3% in healthy person, 55.8% and 70.9% in gastritis, 60.8% and 71.1% in gastric ulcer, and 56.3% and 96.8% in gastric cancer. The isolation rate of H. pylari from patients between 20 and 39 years old was 16.8%, far patients between 40 and 59 years old it was 51.9%, and for patients above 60 years old it was 31.2%. The isolation rate of the vacuolating toxin producing H pylori from gastric biopsy specimens was 66.7% in a healthy person, 76.6% in gastritis, 79.4% in gastric ulcer, and 80% in gastric cancer. Conclusions: The isolation rate of H. pylori from the patients with gastric diseases is higher than the rate of H. pylori from healthy persons, but the isolation rate of the vacuolating toxin producing H. pylori is not different between the patients with gastric diseases and healthy persons. The titers of vacuolating toxin produced by some H, pylori isolated from the patients with gastric diseases are higher than those from healthy persons.